Restriction map for
pFN21AB8955
(Circular) (Six-base) MAPSORT of: pFN21AB8955.seq Check: 6913 from: 1 to: 5701
pFN21AB8955 5701 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3511 278
Size: 53 83 186 936 1975 2468
Fragments arranged by size:
2468 1975 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2307 3044 1937
Size: 370 737 4594
Fragments arranged by size:
4594 737 370
Acc65I G'GTAC_C
Cuts at: 3022 3022
Size: 5701
AclI AA'CG_TT
Cuts at: 1563 3828 4201 1563
Size: 2265 373 3063
Fragments arranged by size:
3063 2265 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2269 2627 3764 5148 5416 1632
Size: 637 358 1137 1384 268 1917
Fragments arranged by size:
1917 1384 1137 637 358 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5504
AflIII A'CryG_T
Cuts at: 1203 4600 5577 1203
Size: 3397 977 1327
Fragments arranged by size:
3397 1327 977
AgeI A'CCGG_T
Cuts at: 4532 4532
Size: 5701
AhdI GACnn_n'nnGTC
Cuts at: 4430 4430
Size: 5701
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5701
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5701
AlwNI CAG_nnn'CTG
Cuts at: 3444 5016 3444
Size: 1572 4129
ApaLI G'TGCA_C
Cuts at: 3758 4914 3758
Size: 1156 4545
ApoI r'AATT_y
Cuts at: 1493 3010 3236 3388 5497 1493
Size: 1517 226 152 2109 1697
Fragments arranged by size:
2109 1697 1517 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4255 160
Size: 872 3223 1606
Fragments arranged by size:
3223 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5701
AvaI C'yCGr_G
Cuts at: 1870 1943 2364 2668 3026 1870
Size: 73 421 304 358 4545
Fragments arranged by size:
4545 421 358 304 73
BamHI G'GATC_C
Cuts at: 3031 3398 3031
Size: 367 5334
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3022 4477 618
Size: 325 217 627 1235 1455 1842
Fragments arranged by size:
1842 1455 1235 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2178 2279 3020 1391
Size: 787 101 741 4072
Fragments arranged by size:
4072 787 741 101
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4730
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2098 2132 3892 3926 1903
Size: 34 50 34 77 34 1760 34 3678
Fragments arranged by size:
3678 1760 77 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3593 4803 3593
Size: 1210 4491
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5701
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2165 4312 136
Size: 107 122 71 631 605 493 2147 1525
Fragments arranged by size:
2147 1525 631 605 493 122 107 71
BglII A'GATC_T
Cuts at: 2616 5696 2616
Size: 3080 2621
BlpI GC'TnA_GC
Cuts at: 3118 3118
Size: 5701
Bme1580I G_kGCm'C
Cuts at: 1790 2105 3762 4918 1790
Size: 315 1657 1156 2573
Fragments arranged by size:
2573 1657 1156 315
BmgBI CAC'GTC
Cuts at: 1318 2164 1318
Size: 846 4855
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3432 4390 442
Size: 416 53 83 399 24 2015 958 1753
Fragments arranged by size:
2015 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5701
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2033 2063 2666 2905 4361 1239
Size: 794 30 603 239 1456 2579
Fragments arranged by size:
2579 1456 794 603 239 30
Bpu10I CC'TnA_GC
Cuts at: 2336 2790 2336
Size: 454 5247
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3826 4691 4989 3826
Size: 865 298 4538
Fragments arranged by size:
4538 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4364 882
Size: 617 2865 2219
Fragments arranged by size:
2865 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2035 3492 493
Size: 711 831 1457 2702
Fragments arranged by size:
2702 1457 831 711
BsaBI GATnn'nnATC
Cuts at: 5695 5695
Size: 5701
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2123 3508 3890 4525
Size: 53 83 186 936 590 1385 382 635
Cuts at: 4525 275
Size: 1451
Fragments arranged by size:
1451 1385 936 635 590 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2880 4134 4532 4806 4953 5364 2880
Size: 1254 398 274 147 411 3217
Fragments arranged by size:
3217 1254 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5476
Fragments arranged by size:
5476 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5701
BseYI C'CCAG_C
Cuts at: 2969 4904 5400 2969
Size: 1935 496 3270
Fragments arranged by size:
3270 1935 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2203 2449 1163
Size: 573 467 246 4415
Fragments arranged by size:
4415 573 467 246
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2160 3151 3912 4940 1540
Size: 275 186 159 991 761 1028 2301
Fragments arranged by size:
2301 1028 991 761 275 186 159
BsiHKAI G_wGCw'C
Cuts at: 2268 2279 3762 3847 4918 2268
Size: 11 1483 85 1071 3051
Fragments arranged by size:
3051 1483 1071 85 11
BsmI GAATG_Cn'
Cuts at: 3212 3305 3212
Size: 93 5608
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2105 2178 2268 2279 3020 3762 3847
Size: 399 315 73 90 11 741 742 85
Cuts at: 3847 4918 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 741 399 315 90 85
73 11
BspEI T'CCGG_A
Cuts at: 5364 5364
Size: 5701
BspHI T'CATG_A
Cuts at: 3590 5320 3590
Size: 1730 3971
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3056 844
Size: 2212 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2335 3588 1394
Size: 429 512 1253 3507
Fragments arranged by size:
3507 1253 512 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2379 2823 4196 4370 65
Size: 1183 1131 444 1373 174 1396
Fragments arranged by size:
1396 1373 1183 1131 444 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4345 4532 1700
Size: 110 2535 187 2869
Fragments arranged by size:
2869 2535 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5701
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5701
BssSI C'ACGA_G
Cuts at: 3761 4773 3761
Size: 1012 4689
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5701
BstBI TT'CG_AA
Cuts at: 4505 4505
Size: 5701
BstEII G'GTnAC_C
Cuts at: 1184 2745 1184
Size: 1561 4140
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2209 2710 2722 2011
Size: 198 501 12 4990
Fragments arranged by size:
4990 501 198 12
BstYI r'GATC_y
Cuts at: 1972 2616 2691 3031 3398 3783 3800 5252 5338
Size: 644 75 340 367 385 17 1452 86
Cuts at: 5338 5350 5696 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 644 385 367 346 340 86
75 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2094 2169 2298 513
Size: 552 1029 75 129 3916
Fragments arranged by size:
3916 1029 552 129 75
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2038 487
Size: 683 56 812 4150
Fragments arranged by size:
4150 812 683 56
BtsI GCAGTG_nn'
Cuts at: 2585 3217 4011 4031 2585
Size: 632 794 20 4255
Fragments arranged by size:
4255 794 632 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5701
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5666
DraI TTT'AAA
Cuts at: 3005 3005
Size: 5701
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4708 809
Size: 507 3392 1802
Fragments arranged by size:
3392 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2157 2166 2295
Size: 54 1425 50 275 18 327 9 129
Cuts at: 2295 2319 2712 2826 3148 4037 5666 8
Size: 24 393 114 322 889 1629 43
Fragments arranged by size:
1629 1425 889 393 327 322 275 129
114 54 50 43 24 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 2157 3148 1537
Size: 275 345 991 4090
Fragments arranged by size:
4090 991 345 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2183 2246 3631 1353
Size: 830 63 1385 3423
Fragments arranged by size:
3423 1385 830 63
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4288 4662 4808 387
Size: 1064 138 2699 374 146 1280
Fragments arranged by size:
2699 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2277 2277
Size: 5701
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2033 2063 2269 2627 2666 2905 3764
Size: 393 401 30 206 358 39 239 859
Cuts at: 3764 4361 5148 5416 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 859 787 597 401 393 358 268
239 206 39 30
EcoO109I rG'GnC_Cy
Cuts at: 2361 2395 2361
Size: 34 5667
EcoRI G'AATT_C
Cuts at: 3010 3388 3010
Size: 378 5323
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2536 2536
Size: 5701
FspI TGC'GCA
Cuts at: 4207 4207
Size: 5701
HaeII r_GCGC'y
Cuts at: 1678 2042 4848 1678
Size: 364 2806 2531
Fragments arranged by size:
2806 2531 364
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4342 4374 4416 4448 1373
Size: 32 135 32 2770 32 42 32 2626
Fragments arranged by size:
2770 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2308 2764 3045 3291 669
Size: 988 281 370 456 281 246 3079
Fragments arranged by size:
3079 988 456 370 281 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5701
HpaI GTT'AAC
Cuts at: 2764 3291 2764
Size: 527 5174
KpnI G_GTAC'C
Cuts at: 3026 3026
Size: 5701
MfeI C'AATT_G
Cuts at: 3300 3300
Size: 5701
MluI A'CGCG_T
Cuts at: 5577 5577
Size: 5701
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4815 4999 1305
Size: 3510 184 2007
Fragments arranged by size:
3510 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2321 2714 2828 10
Size: 54 1425 343 489 393 114 2883
Fragments arranged by size:
2883 1425 489 393 343 114 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2720 3659 4018 4177 518
Size: 648 1554 939 359 159 2042
Fragments arranged by size:
2042 1554 939 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2096 2686 3117 3794 4942 5187 1606
Size: 490 590 431 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 590 490 431 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5149
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5701
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5701
NotI GC'GGCC_GC
Cuts at: 3148 3148
Size: 5701
NspI r_CATG'y
Cuts at: 1132 1229 2759 2846 3059 4604 1132
Size: 97 1530 87 213 1545 2229
Fragments arranged by size:
2229 1545 1530 213 97 87
PasI CC'CwG_GG
Cuts at: 1348 2475 1348
Size: 1127 4574
PciI A'CATG_T
Cuts at: 4600 4600
Size: 5701
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5701
PmeI GTTT'AAAC
Cuts at: 3005 3005
Size: 5701
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3770 3802 5283 5315 3770
Size: 32 1481 32 4156
Fragments arranged by size:
4156 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2361 2395 2361
Size: 34 5667
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5701
PsiI TTA'TAA
Cuts at: 2594 3271 2594
Size: 677 5024
PspXI vC'TCGA_Gb
Cuts at: 2668 2668
Size: 5701
PstI C_TGCA'G
Cuts at: 830 2121 2636 3053 830
Size: 1291 515 417 3478
Fragments arranged by size:
3478 1291 515 417
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5701
SacI G_AGCT'C
Cuts at: 2279 2279
Size: 5701
SacII CC_GC'GG
Cuts at: 2097 2097
Size: 5701
SalI G'TCGA_C
Cuts at: 1936 2306 3043 1936
Size: 370 737 4594
Fragments arranged by size:
4594 737 370
SanDI GG'GwC_CC
Cuts at: 2361 2361
Size: 5701
SapI GCTCTTCn'nnn_
Cuts at: 2183 2183
Size: 5701
SbfI CC_TGCA'GG
Cuts at: 2636 3053 2636
Size: 417 5284
ScaI AGT'ACT
Cuts at: 3949 5661 3949
Size: 1712 3989
SfcI C'TryA_G
Cuts at: 826 1045 2117 2632 3049 4184 4865 5056 826
Size: 219 1072 515 417 1135 681 191 1471
Fragments arranged by size:
1471 1135 1072 681 515 417 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2165 2165
Size: 5701
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5701
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5701
SmaI CCC'GGG
Cuts at: 1872 3028 1872
Size: 1156 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2668 3805 4706 4968 820
Size: 197 926 725 1137 901 262 1553
Fragments arranged by size:
1553 1137 926 901 725 262 197
SnaBI TAC'GTA
Cuts at: 493 3492 493
Size: 2999 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5701
SphI G_CATG'C
Cuts at: 1132 2759 3059 1132
Size: 1627 300 3774
Fragments arranged by size:
3774 1627 300
SspI AAT'ATT
Cuts at: 5 52 3625 5
Size: 47 3573 2081
Fragments arranged by size:
3573 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5701
StyI C'CwwG_G
Cuts at: 513 1065 3140 513
Size: 552 2075 3074
Fragments arranged by size:
3074 2075 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2559 3728 3745 3898 4083 4528 325
Size: 1255 979 1169 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1169 979 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3947 5659 96
Size: 274 80 33 51 1443 1970 1712 138
Fragments arranged by size:
1970 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5661 5693 5661
Size: 32 5669
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5701
XbaI T'CTAG_A
Cuts at: 3037 3037
Size: 5701
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2137 2430 2545 2137
Size: 293 115 5293
Fragments arranged by size:
5293 293 115
XhoI C'TCGA_G
Cuts at: 1943 2668 1943
Size: 725 4976
XmaI C'CCGG_G
Cuts at: 1870 3026 1870
Size: 1156 4545
XmnI GAAnn'nnTTC
Cuts at: 3830 3830
Size: 5701
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3509 276
Size: 53 83 186 936 1975 2468
Fragments arranged by size:
2468 1975 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI
PstI PvuI SacI SacII SalI SanDI SapI SbfI
ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspOMI PsrI PvuII
RsrII SexAI SfoI SrfI SwaI