Restriction map for
pFN21AB8954
(Circular) (Six-base) MAPSORT of: pFN21AB8954.seq Check: 2952 from: 1 to: 5695
pFN21AB8954 5695 bp
With 182 enzymes: SgfI *
December 8, 2014 13:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3505 278
Size: 53 83 186 936 1969 2468
Fragments arranged by size:
2468 1969 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3038 1937
Size: 1101 4594
Acc65I G'GTAC_C
Cuts at: 3016 3016
Size: 5695
AclI AA'CG_TT
Cuts at: 1563 3822 4195 1563
Size: 2259 373 3063
Fragments arranged by size:
3063 2259 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2840 3758 5142 5410 1632
Size: 1208 918 1384 268 1917
Fragments arranged by size:
1917 1384 1208 918 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5498
AflIII A'CryG_T
Cuts at: 1203 2574 2794 4594 5571 1203
Size: 1371 220 1800 977 1327
Fragments arranged by size:
1800 1371 1327 977 220
AgeI A'CCGG_T
Cuts at: 4526 4526
Size: 5695
AhdI GACnn_n'nnGTC
Cuts at: 2497 4424 2497
Size: 1927 3768
AleI CACnn'nnGTG
Cuts at: 1166 2810 1166
Size: 1644 4051
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5695
AlwNI CAG_nnn'CTG
Cuts at: 2193 2208 3438 5010 2193
Size: 15 1230 1572 2878
Fragments arranged by size:
2878 1572 1230 15
ApaLI G'TGCA_C
Cuts at: 3752 4908 3752
Size: 1156 4539
ApoI r'AATT_y
Cuts at: 1493 3004 3230 3382 5491 1493
Size: 1511 226 152 2109 1697
Fragments arranged by size:
2109 1697 1511 226 152
AscI GG'CGCG_CC
Cuts at: 2309 2309
Size: 5695
AseI AT'TA_AT
Cuts at: 160 1032 4249 160
Size: 872 3217 1606
Fragments arranged by size:
3217 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5695
AvaI C'yCGr_G
Cuts at: 1870 1943 2167 2449 2588 2759 3020 1870
Size: 73 224 282 139 171 261 4545
Fragments arranged by size:
4545 282 261 224 171 139 73
BamHI G'GATC_C
Cuts at: 3025 3392 3025
Size: 367 5328
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2261 2273 2477 2584 2601
Size: 325 217 627 474 12 204 107 17
Cuts at: 2601 3016 4471 618
Size: 415 1455 1842
Fragments arranged by size:
1842 1455 627 474 415 325 217 204
107 17 12
BanII G_rGCy'C
Cuts at: 1391 2156 2659 3014 1391
Size: 765 503 355 4072
Fragments arranged by size:
4072 765 503 355
BbeI G_GCGC'C
Cuts at: 2265 2277 2481 2265
Size: 12 204 5479
Fragments arranged by size:
5479 204 12
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4724
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1999 2033 3886 3920 1903
Size: 34 62 34 1853 34 3678
Fragments arranged by size:
3678 1853 62 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3587 4797 3587
Size: 1210 4485
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5695
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2303 4306 136
Size: 107 122 71 631 605 631 2003 1525
Fragments arranged by size:
2003 1525 631 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5690 5690
Size: 5695
BlpI GC'TnA_GC
Cuts at: 3112 3112
Size: 5695
Bme1580I G_kGCm'C
Cuts at: 1790 2323 2589 2604 2802 3756 4912 1790
Size: 533 266 15 198 954 1156 2573
Fragments arranged by size:
2573 1156 954 533 266 198 15
BmgBI CAC'GTC
Cuts at: 1318 2575 2743 1318
Size: 1257 168 4270
Fragments arranged by size:
4270 1257 168
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2662 3426 4384
Size: 416 53 83 399 24 1245 764 958
Cuts at: 4384 442
Size: 1753
Fragments arranged by size:
1753 1245 958 764 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2850 1056
Size: 1794 3901
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4355 1239
Size: 3116 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3820 4685 4983 3820
Size: 865 298 4532
Fragments arranged by size:
4532 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4358 882
Size: 617 2859 2219
Fragments arranged by size:
2859 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3486 493
Size: 711 2282 2702
Fragments arranged by size:
2702 2282 711
BsaBI GATnn'nnATC
Cuts at: 5689 5689
Size: 5695
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2262 2274 2478 2622
Size: 53 83 186 936 729 12 204 144
Cuts at: 2622 3502 3884 4519 275
Size: 880 382 635 1451
Fragments arranged by size:
1451 936 880 729 635 382 204 186
144 83 53 12
BsaWI w'CCGG_w
Cuts at: 4128 4526 4800 4947 5358 4128
Size: 398 274 147 411 4465
Fragments arranged by size:
4465 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5470
Fragments arranged by size:
5470 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2118 2121 1186
Size: 932 3 4760
Fragments arranged by size:
4760 932 3
BseYI C'CCAG_C
Cuts at: 2236 2531 2670 4898 5394 2236
Size: 295 139 2228 496 2537
Fragments arranged by size:
2537 2228 496 295 139
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2834 1163
Size: 573 1098 4024
Fragments arranged by size:
4024 1098 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3145 3906 4934 1540
Size: 275 186 1144 761 1028 2301
Fragments arranged by size:
2301 1144 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3756 3841 4912 3756
Size: 85 1071 4539
Fragments arranged by size:
4539 1071 85
BsmI GAATG_Cn'
Cuts at: 3206 3299 3206
Size: 93 5602
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2156 2323 2589 2604 2659 2802 3014
Size: 399 366 167 266 15 55 143 212
Cuts at: 3014 3756 3841 4912 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 366 266 212 167
143 85 55 15
BspEI T'CCGG_A
Cuts at: 5358 5358
Size: 5695
BspHI T'CATG_A
Cuts at: 3584 5314 3584
Size: 1730 3965
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2226 2430 3050 844
Size: 1382 204 620 3489
Fragments arranged by size:
3489 1382 620 204
BsrBI CCG'CTC
Cuts at: 1394 1823 2082 2129 3582 1394
Size: 429 259 47 1453 3507
Fragments arranged by size:
3507 1453 429 259 47
BsrDI GCAATG_nn'
Cuts at: 65 1248 4190 4364 65
Size: 1183 2942 174 1396
Fragments arranged by size:
2942 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2248 2775 2874 2934 4339 4526 1700
Size: 110 438 527 99 60 1405 187 2869
Fragments arranged by size:
2869 1405 527 438 187 110 99 60
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5695
BssHII G'CGCG_C
Cuts at: 1925 2309 1925
Size: 384 5311
BssSI C'ACGA_G
Cuts at: 3755 4767 3755
Size: 1012 4683
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2193 2233 794
Size: 1399 40 4256
Fragments arranged by size:
4256 1399 40
BstBI TT'CG_AA
Cuts at: 4499 4499
Size: 5695
BstEII G'GTnAC_C
Cuts at: 1184 2914 1184
Size: 1730 3965
BstYI r'GATC_y
Cuts at: 1972 3025 3392 3777 3794 5246 5332 5344 5690
Size: 1053 367 385 17 1452 86 12 346
Cuts at: 5690 1972
Size: 1977
Fragments arranged by size:
1977 1452 1053 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2268 2268
Size: 5695
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5143
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2570 487
Size: 683 56 1344 3612
Fragments arranged by size:
3612 1344 683 56
BtsI GCAGTG_nn'
Cuts at: 2206 3211 4005 4025 2206
Size: 1005 794 20 3876
Fragments arranged by size:
3876 1005 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5695
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5660
DraI TTT'AAA
Cuts at: 2999 2999
Size: 5695
DraIII CAC_nnn'GTG
Cuts at: 2233 2416 2233
Size: 183 5512
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4702 809
Size: 507 3386 1802
Fragments arranged by size:
3386 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2036 2199 3142
Size: 54 1425 50 275 18 206 163 943
Cuts at: 3142 4031 5660 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 943 889 275 206 163 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3142 1537
Size: 275 1330 4090
Fragments arranged by size:
4090 1330 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3625 1353
Size: 2272 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2090 2115 2139 2701 2875 4282
Size: 1064 138 501 25 24 562 174 1407
Cuts at: 4282 4656 4802 387
Size: 374 146 1280
Fragments arranged by size:
1407 1280 1064 562 501 374 174 146
138 25 24
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2840 3758 4355 5142 5410 1239
Size: 393 1208 918 597 787 268 1524
Fragments arranged by size:
1524 1208 918 787 597 393 268
EcoRI G'AATT_C
Cuts at: 3004 3382 3004
Size: 378 5317
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2749 2749
Size: 5695
FspI TGC'GCA
Cuts at: 4201 4201
Size: 5695
HaeII r_GCGC'y
Cuts at: 1678 2265 2277 2355 2481 2875 2911 2978 4842
Size: 587 12 78 126 394 36 67 1864
Cuts at: 4842 1678
Size: 2531
Fragments arranged by size:
2531 1864 587 394 126 78 67 36
12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2483 2486 2515 2518 4336
Size: 32 135 32 911 3 29 3 1818
Cuts at: 4336 4368 4410 4442 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1818 911 135 42 32 32 32
32 29 3 3
HincII GTy'rAC
Cuts at: 669 1657 1938 3039 3285 669
Size: 988 281 1101 246 3079
Fragments arranged by size:
3079 1101 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5695
HpaI GTT'AAC
Cuts at: 3285 3285
Size: 5695
KasI G'GCGC_C
Cuts at: 2261 2273 2477 2261
Size: 12 204 5479
Fragments arranged by size:
5479 204 12
KpnI G_GTAC'C
Cuts at: 3020 3020
Size: 5695
MfeI C'AATT_G
Cuts at: 3294 3294
Size: 5695
MluI A'CGCG_T
Cuts at: 2794 5571 2794
Size: 2777 2918
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4809 4993 1305
Size: 3504 184 2007
Fragments arranged by size:
3504 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3873
Fragments arranged by size:
3873 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2810 3653 4012 4171 518
Size: 648 1644 843 359 159 2042
Fragments arranged by size:
2042 1644 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2145 2193 2598 2818 3111 3788 4936 5181
Size: 539 48 405 220 293 677 1148 245
Cuts at: 5181 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 539 405 293 245 220
48
NaeI GCC'GGC
Cuts at: 2250 2876 2250
Size: 626 5069
NarI GG'CG_CC
Cuts at: 2262 2274 2478 2262
Size: 12 204 5479
Fragments arranged by size:
5479 204 12
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5143
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5695
NgoMIV G'CCGG_C
Cuts at: 2248 2874 2248
Size: 626 5069
NheI G'CTAG_C
Cuts at: 1052 2846 1052
Size: 1794 3901
NotI GC'GGCC_GC
Cuts at: 3142 3142
Size: 5695
NspI r_CATG'y
Cuts at: 1132 1229 3053 4598 1132
Size: 97 1824 1545 2229
Fragments arranged by size:
2229 1824 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2257 2359 1348
Size: 909 102 4684
Fragments arranged by size:
4684 909 102
PciI A'CATG_T
Cuts at: 4594 4594
Size: 5695
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2670 1108
Size: 1562 4133
PmeI GTTT'AAAC
Cuts at: 2999 2999
Size: 5695
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3764 3796 5277 5309 3764
Size: 32 1481 32 4150
Fragments arranged by size:
4150 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5695
PsiI TTA'TAA
Cuts at: 3265 3265
Size: 5695
PstI C_TGCA'G
Cuts at: 830 2144 2427 2757 3047 830
Size: 1314 283 330 290 3478
Fragments arranged by size:
3478 1314 330 290 283
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5695
PvuII CAG'CTG
Cuts at: 2193 2818 2193
Size: 625 5070
SalI G'TCGA_C
Cuts at: 1936 3037 1936
Size: 1101 4594
SbfI CC_TGCA'GG
Cuts at: 3047 3047
Size: 5695
ScaI AGT'ACT
Cuts at: 3943 5655 3943
Size: 1712 3983
SfcI C'TryA_G
Cuts at: 826 1045 2140 2423 2753 3043 4178 4859 5050
Size: 219 1095 283 330 290 1135 681 191
Cuts at: 5050 826
Size: 1471
Fragments arranged by size:
1471 1135 1095 681 330 290 283 219
191
SfoI GGC'GCC
Cuts at: 2263 2275 2479 2263
Size: 12 204 5479
Fragments arranged by size:
5479 204 12
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5695
SgrAI Cr'CCGG_yG
Cuts at: 1700 2874 1700
Size: 1174 4521
SmaI CCC'GGG
Cuts at: 1872 2451 2590 3022 1872
Size: 579 139 432 4545
Fragments arranged by size:
4545 579 432 139
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3799 4700 4962 820
Size: 197 926 1856 901 262 1553
Fragments arranged by size:
1856 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3486 493
Size: 2993 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5695
SphI G_CATG'C
Cuts at: 1132 3053 1132
Size: 1921 3774
SspI AAT'ATT
Cuts at: 5 52 3619 5
Size: 47 3567 2081
Fragments arranged by size:
3567 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5695
StyI C'CwwG_G
Cuts at: 513 1065 2511 2694 3134 513
Size: 552 1446 183 440 3074
Fragments arranged by size:
3074 1446 552 440 183
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3722 3739 3892 4077 4522 325
Size: 1255 2142 17 153 185 445 1498
Fragments arranged by size:
2142 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2685 3941 5653
Size: 274 80 33 51 1443 708 1256 1712
Cuts at: 5653 96
Size: 138
Fragments arranged by size:
1712 1443 1256 708 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5655 5687 5655
Size: 32 5663
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5695
XbaI T'CTAG_A
Cuts at: 3031 3031
Size: 5695
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2356 2356
Size: 5695
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5695
XmaI C'CCGG_G
Cuts at: 1870 2449 2588 3020 1870
Size: 579 139 432 4545
Fragments arranged by size:
4545 579 432 139
XmnI GAAnn'nnTTC
Cuts at: 3824 3824
Size: 5695
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3503 276
Size: 53 83 186 936 1969 2468
Fragments arranged by size:
2468 1969 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI
EcoO109I EcoRV FseI FspAI NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SrfI SwaI