Restriction map for
pFN21AB8953
(Circular) (Six-base) MAPSORT of: pFN21AB8953.seq Check: 4974 from: 1 to: 5689
pFN21AB8953 5689 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2302 2302
Size: 5689
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3499 278
Size: 53 83 186 936 1963 2468
Fragments arranged by size:
2468 1963 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3032 1937
Size: 1095 4594
Acc65I G'GTAC_C
Cuts at: 3010 3010
Size: 5689
AclI AA'CG_TT
Cuts at: 1563 3816 4189 1563
Size: 2253 373 3063
Fragments arranged by size:
3063 2253 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2593 3752 5136 5404 1632
Size: 961 1159 1384 268 1917
Fragments arranged by size:
1917 1384 1159 961 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5492
AflIII A'CryG_T
Cuts at: 1203 2025 4588 5565 1203
Size: 822 2563 977 1327
Fragments arranged by size:
2563 1327 977 822
AgeI A'CCGG_T
Cuts at: 4520 4520
Size: 5689
AhdI GACnn_n'nnGTC
Cuts at: 2389 4418 2389
Size: 2029 3660
AleI CACnn'nnGTG
Cuts at: 1166 2529 1166
Size: 1363 4326
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5689
AlwNI CAG_nnn'CTG
Cuts at: 3432 5004 3432
Size: 1572 4117
ApaLI G'TGCA_C
Cuts at: 2257 3746 4902 2257
Size: 1489 1156 3044
Fragments arranged by size:
3044 1489 1156
ApoI r'AATT_y
Cuts at: 1493 2707 2998 3224 3376 5485 1493
Size: 1214 291 226 152 2109 1697
Fragments arranged by size:
2109 1697 1214 291 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4243 160
Size: 872 3211 1606
Fragments arranged by size:
3211 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5689
AvaI C'yCGr_G
Cuts at: 1870 1943 2356 2425 2769 3014 1870
Size: 73 413 69 344 245 4545
Fragments arranged by size:
4545 413 344 245 73 69
BamHI G'GATC_C
Cuts at: 3019 3386 3019
Size: 367 5322
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2074 2147 2285 2561 3010
Size: 325 217 627 287 73 138 276 449
Cuts at: 3010 4465 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 449 325 287 276 217
138 73
BanII G_rGCy'C
Cuts at: 1391 2012 2776 3008 1391
Size: 621 764 232 4072
Fragments arranged by size:
4072 764 621 232
BbeI G_GCGC'C
Cuts at: 2151 2151
Size: 5689
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2413 928
Size: 971 514 4204
Fragments arranged by size:
4204 971 514
BbvCI CC'TCA_GC
Cuts at: 2447 2447
Size: 5689
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2267 2301 3880 3914 1903
Size: 34 330 34 1579 34 3678
Fragments arranged by size:
3678 1579 330 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3581 4791 3581
Size: 1210 4479
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5689
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2414 4300 136
Size: 107 122 71 631 605 742 1886 1525
Fragments arranged by size:
1886 1525 742 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5684 5684
Size: 5689
BlpI GC'TnA_GC
Cuts at: 2066 3106 2066
Size: 1040 4649
Bme1580I G_kGCm'C
Cuts at: 1790 2261 3750 4906 1790
Size: 471 1489 1156 2573
Fragments arranged by size:
2573 1489 1156 471
BmgBI CAC'GTC
Cuts at: 1318 2062 2398 1318
Size: 744 336 4609
Fragments arranged by size:
4609 744 336
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2239 3420 4378
Size: 416 53 83 399 24 822 1181 958
Cuts at: 4378 442
Size: 1753
Fragments arranged by size:
1753 1181 958 822 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2685 1056
Size: 1629 4060
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2039 2285 2674 4349 1239
Size: 800 246 389 1675 2579
Fragments arranged by size:
2579 1675 800 389 246
Bpu10I CC'TnA_GC
Cuts at: 2447 2447
Size: 5689
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3814 4679 4977 3814
Size: 865 298 4526
Fragments arranged by size:
4526 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2397 4352 882
Size: 617 898 1955 2219
Fragments arranged by size:
2219 1955 898 617
BsaAI yAC'GTr
Cuts at: 493 1204 2026 3480 493
Size: 711 822 1454 2702
Fragments arranged by size:
2702 1454 822 711
BsaBI GATnn'nnATC
Cuts at: 5683 5683
Size: 5689
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2097 2148 3496 3878
Size: 53 83 186 936 564 51 1348 382
Cuts at: 3878 4513 275
Size: 635 1451
Fragments arranged by size:
1451 1348 936 635 564 382 186 83
53 51
BsaWI w'CCGG_w
Cuts at: 4122 4520 4794 4941 5352 4122
Size: 398 274 147 411 4459
Fragments arranged by size:
4459 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2012 2042 1178
Size: 30 165 30 609 30 4825
Fragments arranged by size:
4825 609 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2435 1186
Size: 1249 4440
BseYI C'CCAG_C
Cuts at: 2306 2365 4892 5388 2306
Size: 59 2527 496 2607
Fragments arranged by size:
2607 2527 496 59
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5116
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2205 2219 2238 2379 2418 3139
Size: 275 186 204 14 19 141 39 721
Cuts at: 3139 3900 4928 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1028 761 721 275 204 186 141
39 19 14
BsiHKAI G_wGCw'C
Cuts at: 2012 2067 2261 3750 3835 4906 2012
Size: 55 194 1489 85 1071 2795
Fragments arranged by size:
2795 1489 1071 194 85 55
BsiWI C'GTAC_G
Cuts at: 2346 2346
Size: 5689
BsmI GAATG_Cn'
Cuts at: 2647 3200 3293 2647
Size: 553 93 5043
Fragments arranged by size:
5043 553 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2584 2584
Size: 5689
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2067 2261 2776 3008 3750 3835
Size: 399 222 55 194 515 232 742 85
Cuts at: 3835 4906 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 515 399 232 222 194
85 55
BspEI T'CCGG_A
Cuts at: 5352 5352
Size: 5689
BspHI T'CATG_A
Cuts at: 3578 5308 3578
Size: 1730 3959
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2302 3044 844
Size: 1458 742 3489
Fragments arranged by size:
3489 1458 742
BsrBI CCG'CTC
Cuts at: 1394 1823 2091 3576 1394
Size: 429 268 1485 3507
Fragments arranged by size:
3507 1485 429 268
BsrDI GCAATG_nn'
Cuts at: 65 1248 4184 4358 65
Size: 1183 2936 174 1396
Fragments arranged by size:
2936 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2341 4333 4520 1700
Size: 110 531 1992 187 2869
Fragments arranged by size:
2869 1992 531 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5689
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5689
BssSI C'ACGA_G
Cuts at: 3749 4761 3749
Size: 1012 4677
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5689
BstBI TT'CG_AA
Cuts at: 4493 4493
Size: 5689
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5689
BstXI CCAn_nnnn'nTGG
Cuts at: 2899 2899
Size: 5689
BstYI r'GATC_y
Cuts at: 1972 3019 3386 3771 3788 5240 5326 5338 5684
Size: 1047 367 385 17 1452 86 12 346
Cuts at: 5684 1972
Size: 1977
Fragments arranged by size:
1977 1452 1047 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2106 2298 513
Size: 552 1041 192 3904
Fragments arranged by size:
3904 1041 552 192
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4950
Fragments arranged by size:
4950 683 56
BtsI GCAGTG_nn'
Cuts at: 2261 3205 3999 4019 2261
Size: 944 794 20 3931
Fragments arranged by size:
3931 944 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5689
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5654
DraI TTT'AAA
Cuts at: 2993 2993
Size: 5689
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2819 4696 809
Size: 507 1503 1877 1802
Fragments arranged by size:
1877 1802 1503 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2172 2202 2216
Size: 54 1425 50 275 18 342 30 14
Cuts at: 2216 2235 2376 2415 2452 2521 2883 3136 4025
Size: 19 141 39 37 69 362 253 889
Cuts at: 4025 5654 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 362 342 275 253 141
69 54 50 43 39 37 30 19
18 14
EagI C'GGCC_G
Cuts at: 1537 1812 2202 2216 2235 2376 2415 3136 1537
Size: 275 390 14 19 141 39 721 4090
Fragments arranged by size:
4090 721 390 275 141 39 19 14
EarI CTCTTCn'nnn_
Cuts at: 1353 2630 3619 1353
Size: 1277 989 3423
Fragments arranged by size:
3423 1277 989
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4276 4650 4796 387
Size: 1064 138 2687 374 146 1280
Fragments arranged by size:
2687 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2010 2010
Size: 5689
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2039 2285 2593 2674 3752 4349 5136
Size: 393 407 246 308 81 1078 597 787
Cuts at: 5136 5404 1239
Size: 268 1524
Fragments arranged by size:
1524 1078 787 597 407 393 308 268
246 81
EcoO109I rG'GnC_Cy
Cuts at: 2271 2271
Size: 5689
EcoRI G'AATT_C
Cuts at: 2998 3376 2998
Size: 378 5311
FspI TGC'GCA
Cuts at: 4195 4195
Size: 5689
HaeII r_GCGC'y
Cuts at: 1678 2151 4836 1678
Size: 473 2685 2531
Fragments arranged by size:
2685 2531 473
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2012 2044 2375 2407 4330
Size: 32 135 32 440 32 331 32 1923
Cuts at: 4330 4362 4404 4436 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1923 440 331 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3033 3279 669
Size: 988 281 1095 246 3079
Fragments arranged by size:
3079 1095 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5689
HpaI GTT'AAC
Cuts at: 3279 3279
Size: 5689
KasI G'GCGC_C
Cuts at: 2147 2147
Size: 5689
KpnI G_GTAC'C
Cuts at: 3014 3014
Size: 5689
MfeI C'AATT_G
Cuts at: 3288 3288
Size: 5689
MluI A'CGCG_T
Cuts at: 5565 5565
Size: 5689
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2118 2935 4803 4987 1305
Size: 813 817 1868 184 2007
Fragments arranged by size:
2007 1868 817 813 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2885 10
Size: 54 1425 343 1053 2814
Fragments arranged by size:
2814 1425 1053 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2529 2808 2897 3647 4006 4165 518
Size: 648 1363 279 89 750 359 159 2042
Fragments arranged by size:
2042 1363 750 648 359 279 159 89
MspA1I CmG'CkG
Cuts at: 1606 2043 2160 2300 2306 2451 3105 3782 4930
Size: 437 117 140 6 145 654 677 1148
Cuts at: 4930 5175 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 654 437 245 145 140
117 6
NaeI GCC'GGC
Cuts at: 2343 2343
Size: 5689
NarI GG'CG_CC
Cuts at: 2148 2148
Size: 5689
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5137
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5689
NgoMIV G'CCGG_C
Cuts at: 2341 2341
Size: 5689
NheI G'CTAG_C
Cuts at: 1052 2681 1052
Size: 1629 4060
NotI GC'GGCC_GC
Cuts at: 3136 3136
Size: 5689
NspI r_CATG'y
Cuts at: 1132 1229 3047 4592 1132
Size: 97 1818 1545 2229
Fragments arranged by size:
2229 1818 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5689
PciI A'CATG_T
Cuts at: 4588 4588
Size: 5689
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5689
PmeI GTTT'AAAC
Cuts at: 2993 2993
Size: 5689
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3758 3790 5271 5303 3758
Size: 32 1481 32 4144
Fragments arranged by size:
4144 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5689
PsiI TTA'TAA
Cuts at: 3259 3259
Size: 5689
PspXI vC'TCGA_Gb
Cuts at: 2769 2769
Size: 5689
PstI C_TGCA'G
Cuts at: 830 2118 3041 830
Size: 1288 923 3478
Fragments arranged by size:
3478 1288 923
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5689
PvuII CAG'CTG
Cuts at: 2160 2306 2160
Size: 146 5543
SacI G_AGCT'C
Cuts at: 2012 2012
Size: 5689
SacII CC_GC'GG
Cuts at: 2301 2301
Size: 5689
SalI G'TCGA_C
Cuts at: 1936 3031 1936
Size: 1095 4594
SbfI CC_TGCA'GG
Cuts at: 3041 3041
Size: 5689
ScaI AGT'ACT
Cuts at: 2762 3937 5649 2762
Size: 1175 1712 2802
Fragments arranged by size:
2802 1712 1175
SexAI A'CCwGG_T
Cuts at: 2281 2281
Size: 5689
SfcI C'TryA_G
Cuts at: 826 1045 2114 3037 4172 4853 5044 826
Size: 219 1069 923 1135 681 191 1471
Fragments arranged by size:
1471 1135 1069 923 681 219 191
SfoI GGC'GCC
Cuts at: 2149 2149
Size: 5689
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5689
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5689
SmaI CCC'GGG
Cuts at: 1872 2358 3016 1872
Size: 486 658 4545
Fragments arranged by size:
4545 658 486
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2769 3793 4694 4956 820
Size: 197 926 826 1024 901 262 1553
Fragments arranged by size:
1553 1024 926 901 826 262 197
SnaBI TAC'GTA
Cuts at: 493 3480 493
Size: 2987 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5689
SphI G_CATG'C
Cuts at: 1132 3047 1132
Size: 1915 3774
SspI AAT'ATT
Cuts at: 5 52 3613 5
Size: 47 3561 2081
Fragments arranged by size:
3561 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5689
StyI C'CwwG_G
Cuts at: 513 1065 3128 513
Size: 552 2063 3074
Fragments arranged by size:
3074 2063 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2092 3716 3733 3886 4071 4516 325
Size: 1255 512 1624 17 153 185 445 1498
Fragments arranged by size:
1624 1498 1255 512 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2760 3935 5647
Size: 274 80 33 51 1443 783 1175 1712
Cuts at: 5647 96
Size: 138
Fragments arranged by size:
1712 1443 1175 783 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2878 2910 5649 5681 2878
Size: 32 2739 32 2886
Fragments arranged by size:
2886 2739 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5689
XbaI T'CTAG_A
Cuts at: 3025 3025
Size: 5689
XhoI C'TCGA_G
Cuts at: 1943 2769 1943
Size: 826 4863
XmaI C'CCGG_G
Cuts at: 1870 2356 3014 1870
Size: 486 658 4545
Fragments arranged by size:
4545 658 486
XmnI GAAnn'nnTTC
Cuts at: 2877 3818 2877
Size: 941 4748
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3497 276
Size: 53 83 186 936 1963 2468
Fragments arranged by size:
2468 1963 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI
PvuI PvuII SacI SacII SalI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI
BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PsrI
RsrII SanDI SapI SfiI SrfI SwaI XcmI