Restriction map for
pFN21AB8949
(Circular) (Six-base) MAPSORT of: pFN21AB8949.seq Check: 6295 from: 1 to: 5677
pFN21AB8949 5677 bp
With 182 enzymes: SgfI *
August 16, 2010 14:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3487 278
Size: 53 83 186 936 1951 2468
Fragments arranged by size:
2468 1951 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2652 3020 1937
Size: 715 368 4594
Fragments arranged by size:
4594 715 368
Acc65I G'GTAC_C
Cuts at: 2998 2998
Size: 5677
AclI AA'CG_TT
Cuts at: 1563 3804 4177 1563
Size: 2241 373 3063
Fragments arranged by size:
3063 2241 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2582 3740 5124 5392 1632
Size: 950 1158 1384 268 1917
Fragments arranged by size:
1917 1384 1158 950 268
AfeI AGC'GCT
Cuts at: 2035 2035
Size: 5677
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5480
AflIII A'CryG_T
Cuts at: 1203 2966 4576 5553 1203
Size: 1763 1610 977 1327
Fragments arranged by size:
1763 1610 1327 977
AgeI A'CCGG_T
Cuts at: 4508 4508
Size: 5677
AhdI GACnn_n'nnGTC
Cuts at: 4406 4406
Size: 5677
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5677
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2757 1184
Size: 1573 4104
AlwNI CAG_nnn'CTG
Cuts at: 2648 2889 3420 4992 2648
Size: 241 531 1572 3333
Fragments arranged by size:
3333 1572 531 241
ApaI G_GGCC'C
Cuts at: 2132 2177 2132
Size: 45 5632
ApaLI G'TGCA_C
Cuts at: 3734 4890 3734
Size: 1156 4521
ApoI r'AATT_y
Cuts at: 1493 2986 3212 3364 5473 1493
Size: 1493 226 152 2109 1697
Fragments arranged by size:
2109 1697 1493 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4231 160
Size: 872 3199 1606
Fragments arranged by size:
3199 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5677
AvaI C'yCGr_G
Cuts at: 1870 1943 2170 3002 1870
Size: 73 227 832 4545
Fragments arranged by size:
4545 832 227 73
BamHI G'GATC_C
Cuts at: 3007 3374 3007
Size: 367 5310
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2146 2295 2351 2762 2998
Size: 325 217 627 359 149 56 411 236
Cuts at: 2998 4453 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 411 359 325 236 217
149 56
BanII G_rGCy'C
Cuts at: 1391 2132 2177 2996 1391
Size: 741 45 819 4072
Fragments arranged by size:
4072 819 741 45
BbeI G_GCGC'C
Cuts at: 2355 2355
Size: 5677
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2484 2613 928
Size: 971 585 129 3992
Fragments arranged by size:
3992 971 585 129
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2768 2802 3868 3902 1903
Size: 34 831 34 1066 34 3678
Fragments arranged by size:
3678 1066 831 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3569 4779 3569
Size: 1210 4467
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5677
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2159 2181 4288
Size: 107 122 71 631 605 487 22 2107
Cuts at: 4288 136
Size: 1525
Fragments arranged by size:
2107 1525 631 605 487 122 107 71
22
BglII A'GATC_T
Cuts at: 5672 5672
Size: 5677
BlpI GC'TnA_GC
Cuts at: 3094 3094
Size: 5677
Bme1580I G_kGCm'C
Cuts at: 1790 2132 2151 2177 2298 3738 4894 1790
Size: 342 19 26 121 1440 1156 2573
Fragments arranged by size:
2573 1440 1156 342 121 26 19
BmgBI CAC'GTC
Cuts at: 1318 2343 1318
Size: 1025 4652
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2665 3408 4366
Size: 416 53 83 399 24 1248 743 958
Cuts at: 4366 442
Size: 1753
Fragments arranged by size:
1753 1248 958 743 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5677
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2245 2245
Size: 5677
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2723 2819 4337 1239
Size: 1484 96 1518 2579
Fragments arranged by size:
2579 1518 1484 96
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3802 4667 4965 3802
Size: 865 298 4514
Fragments arranged by size:
4514 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4340 882
Size: 617 2841 2219
Fragments arranged by size:
2841 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3468 493
Size: 711 2264 2702
Fragments arranged by size:
2702 2264 711
BsaBI GATnn'nnATC
Cuts at: 5671 5671
Size: 5677
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2352 2360 3484 3866
Size: 53 83 186 936 819 8 1124 382
Cuts at: 3866 4501 275
Size: 635 1451
Fragments arranged by size:
1451 1124 936 819 635 382 186 83
53 8
BsaWI w'CCGG_w
Cuts at: 2245 4110 4508 4782 4929 5340 2245
Size: 1865 398 274 147 411 2582
Fragments arranged by size:
2582 1865 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5452
Fragments arranged by size:
5452 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5677
BseYI C'CCAG_C
Cuts at: 2030 2073 2363 4880 5376 2030
Size: 43 290 2517 496 2331
Fragments arranged by size:
2517 2331 496 290 43
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2365 2435 1163
Size: 573 629 70 4405
Fragments arranged by size:
4405 629 573 70
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2106 3127 3888 4916 1540
Size: 275 186 105 1021 761 1028 2301
Fragments arranged by size:
2301 1028 1021 761 275 186 105
BsiHKAI G_wGCw'C
Cuts at: 3738 3823 4894 3738
Size: 85 1071 4521
Fragments arranged by size:
4521 1071 85
BsmI GAATG_Cn'
Cuts at: 3188 3281 3188
Size: 93 5584
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2132 2151 2177 2298 2996 3738 3823
Size: 399 342 19 26 121 698 742 85
Cuts at: 3823 4894 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 698 399 342 121 85
26 19
BspEI T'CCGG_A
Cuts at: 5340 5340
Size: 5677
BspHI T'CATG_A
Cuts at: 2854 2877 3566 5296 2854
Size: 23 689 1730 3235
Fragments arranged by size:
3235 1730 689 23
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3032 844
Size: 2188 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3564 1394
Size: 429 1741 3507
Fragments arranged by size:
3507 1741 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4172 4346 65
Size: 1183 2924 174 1396
Fragments arranged by size:
2924 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2024 2195 2814 4321 4508 1700
Size: 110 214 171 619 1507 187 2869
Fragments arranged by size:
2869 1507 619 214 187 171 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5677
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5677
BssSI C'ACGA_G
Cuts at: 2219 3737 4749 2219
Size: 1518 1012 3147
Fragments arranged by size:
3147 1518 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5677
BstBI TT'CG_AA
Cuts at: 4481 4481
Size: 5677
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5677
BstYI r'GATC_y
Cuts at: 1972 3007 3374 3759 3776 5228 5314 5326 5672
Size: 1035 367 385 17 1452 86 12 346
Cuts at: 5672 1972
Size: 1977
Fragments arranged by size:
1977 1452 1035 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2142 2154 2430 513
Size: 552 1077 12 276 3760
Fragments arranged by size:
3760 1077 552 276 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4938
Fragments arranged by size:
4938 683 56
BtsI GCAGTG_nn'
Cuts at: 2288 3193 3987 4007 2288
Size: 905 794 20 3958
Fragments arranged by size:
3958 905 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5677
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5642
DraI TTT'AAA
Cuts at: 2981 2981
Size: 5677
DraIII CAC_nnn'GTG
Cuts at: 2302 2302
Size: 5677
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4684 809
Size: 507 3368 1802
Fragments arranged by size:
3368 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2103 2680 2871
Size: 54 1425 50 275 18 273 577 191
Cuts at: 2871 3124 4013 5642 8
Size: 253 889 1629 43
Fragments arranged by size:
1629 1425 889 577 275 273 253 191
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2103 3124 1537
Size: 275 291 1021 4090
Fragments arranged by size:
4090 1021 291 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2240 3607 1353
Size: 887 1367 3423
Fragments arranged by size:
3423 1367 887
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2833 4264 4638 4784 387
Size: 1064 138 1244 1431 374 146 1280
Fragments arranged by size:
1431 1280 1244 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2582 2723 2819 3740 4337 5124 5392
Size: 393 950 141 96 921 597 787 268
Cuts at: 5392 1239
Size: 1524
Fragments arranged by size:
1524 950 921 787 597 393 268 141
96
EcoO109I rG'GnC_Cy
Cuts at: 2129 2174 2541 2129
Size: 45 367 5265
Fragments arranged by size:
5265 367 45
EcoRI G'AATT_C
Cuts at: 2986 3364 2986
Size: 378 5299
FseI GG_CCGG'CC
Cuts at: 2199 2199
Size: 5677
FspI TGC'GCA
Cuts at: 4183 4183
Size: 5677
HaeII r_GCGC'y
Cuts at: 1678 2037 2355 4824 1678
Size: 359 318 2469 2531
Fragments arranged by size:
2531 2469 359 318
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2548 2580 2595 2627 4318
Size: 32 135 32 976 32 15 32 1691
Cuts at: 4318 4350 4392 4424 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1691 976 135 42 32 32 32
32 32 32 15
HincII GTy'rAC
Cuts at: 669 1657 1938 2653 3021 3267 669
Size: 988 281 715 368 246 3079
Fragments arranged by size:
3079 988 715 368 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5677
HpaI GTT'AAC
Cuts at: 3267 3267
Size: 5677
KasI G'GCGC_C
Cuts at: 2351 2351
Size: 5677
KpnI G_GTAC'C
Cuts at: 3002 3002
Size: 5677
MfeI C'AATT_G
Cuts at: 3276 3276
Size: 5677
MluI A'CGCG_T
Cuts at: 5553 5553
Size: 5677
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4791 4975 1305
Size: 3486 184 2007
Fragments arranged by size:
3486 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2682 10
Size: 54 1425 343 850 3005
Fragments arranged by size:
3005 1425 850 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2696 2859 3635 3994 4153 518
Size: 648 1530 163 776 359 159 2042
Fragments arranged by size:
2042 1530 776 648 359 163 159
MspA1I CmG'CkG
Cuts at: 1606 2144 2156 3093 3770 4918 5163 1606
Size: 538 12 937 677 1148 245 2120
Fragments arranged by size:
2120 1148 937 677 538 245 12
NaeI GCC'GGC
Cuts at: 2197 2197
Size: 5677
NarI GG'CG_CC
Cuts at: 2352 2352
Size: 5677
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5125
NdeI CA'TA_TG
Cuts at: 387 2483 387
Size: 2096 3581
NgoMIV G'CCGG_C
Cuts at: 2195 2195
Size: 5677
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5677
NotI GC'GGCC_GC
Cuts at: 3124 3124
Size: 5677
NspI r_CATG'y
Cuts at: 1132 1229 2970 3035 4580 1132
Size: 97 1741 65 1545 2229
Fragments arranged by size:
2229 1741 1545 97 65
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5677
PciI A'CATG_T
Cuts at: 2966 4576 2966
Size: 1610 4067
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2516 1108
Size: 1408 4269
PmeI GTTT'AAAC
Cuts at: 2981 2981
Size: 5677
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3746 3778 5259 5291 3746
Size: 32 1481 32 4132
Fragments arranged by size:
4132 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2541 2541
Size: 5677
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5677
PsiI TTA'TAA
Cuts at: 3247 3247
Size: 5677
PspOMI G'GGCC_C
Cuts at: 2128 2173 2128
Size: 45 5632
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2774 2806 2774
Size: 32 5645
PstI C_TGCA'G
Cuts at: 830 2051 2283 3029 830
Size: 1221 232 746 3478
Fragments arranged by size:
3478 1221 746 232
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5677
SacII CC_GC'GG
Cuts at: 2145 2157 2145
Size: 12 5665
SalI G'TCGA_C
Cuts at: 1936 2651 3019 1936
Size: 715 368 4594
Fragments arranged by size:
4594 715 368
SanDI GG'GwC_CC
Cuts at: 2541 2541
Size: 5677
SapI GCTCTTCn'nnn_
Cuts at: 2240 2240
Size: 5677
SbfI CC_TGCA'GG
Cuts at: 3029 3029
Size: 5677
ScaI AGT'ACT
Cuts at: 3925 5637 3925
Size: 1712 3965
SexAI A'CCwGG_T
Cuts at: 2298 2298
Size: 5677
SfcI C'TryA_G
Cuts at: 826 1045 2047 2279 2625 2791 3025 4160 4841
Size: 219 1002 232 346 166 234 1135 681
Cuts at: 4841 5032 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1002 681 346 234 232 219
191 166
SfoI GGC'GCC
Cuts at: 2353 2353
Size: 5677
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5677
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5677
SmaI CCC'GGG
Cuts at: 1872 3004 1872
Size: 1132 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3781 4682 4944 820
Size: 197 926 1838 901 262 1553
Fragments arranged by size:
1838 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3468 493
Size: 2975 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5677
SphI G_CATG'C
Cuts at: 1132 3035 1132
Size: 1903 3774
SspI AAT'ATT
Cuts at: 5 52 3601 5
Size: 47 3549 2081
Fragments arranged by size:
3549 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5677
StyI C'CwwG_G
Cuts at: 513 1065 2941 3116 513
Size: 552 1876 175 3074
Fragments arranged by size:
3074 1876 552 175
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2647 3704 3721 3874 4059 4504 325
Size: 1255 1067 1057 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1067 1057 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3923 5635 96
Size: 274 80 33 51 1443 1946 1712 138
Fragments arranged by size:
1946 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2069 2101 5637 5669 2069
Size: 32 3536 32 2077
Fragments arranged by size:
3536 2077 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2261 2612 1316
Size: 945 351 4381
Fragments arranged by size:
4381 945 351
XbaI T'CTAG_A
Cuts at: 3013 3013
Size: 5677
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5677
XmaI C'CCGG_G
Cuts at: 1870 3002 1870
Size: 1132 4545
XmnI GAAnn'nnTTC
Cuts at: 3806 3806
Size: 5677
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3485 276
Size: 53 83 186 936 1951 2468
Fragments arranged by size:
2468 1951 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FseI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI
PvuI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV
FalI FspAI NruI NsiI PacI PfoI PmlI PspXI
PvuII RsrII SacI SfiI SrfI SwaI XcmI