Restriction map for
pFN21AB8948
(Circular) (Six-base) MAPSORT of: pFN21AB8948.seq Check: 7762 from: 1 to: 5653
pFN21AB8948 5653 bp
With 182 enzymes: SgfI *
August 14, 2012 18:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2281 2281
Size: 5653
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2752 3463 278
Size: 53 83 186 936 1216 711 2468
Fragments arranged by size:
2468 1216 936 711 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2996 1937
Size: 1059 4594
Acc65I G'GTAC_C
Cuts at: 2974 2974
Size: 5653
AclI AA'CG_TT
Cuts at: 1563 3780 4153 1563
Size: 2217 373 3063
Fragments arranged by size:
3063 2217 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3716 5100 5368 1632
Size: 2084 1384 268 1917
Fragments arranged by size:
2084 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5456
AflIII A'CryG_T
Cuts at: 1203 2013 4552 5529 1203
Size: 810 2539 977 1327
Fragments arranged by size:
2539 1327 977 810
AgeI A'CCGG_T
Cuts at: 4484 4484
Size: 5653
AhdI GACnn_n'nnGTC
Cuts at: 4382 4382
Size: 5653
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5653
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2963 1184
Size: 1779 3874
AlwNI CAG_nnn'CTG
Cuts at: 2091 2855 3396 4968 2091
Size: 764 541 1572 2776
Fragments arranged by size:
2776 1572 764 541
ApaI G_GGCC'C
Cuts at: 2225 2225
Size: 5653
ApaLI G'TGCA_C
Cuts at: 3710 4866 3710
Size: 1156 4497
ApoI r'AATT_y
Cuts at: 1493 2962 3188 3340 5449 1493
Size: 1469 226 152 2109 1697
Fragments arranged by size:
2109 1697 1469 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4207 160
Size: 872 3175 1606
Fragments arranged by size:
3175 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5653
AvaI C'yCGr_G
Cuts at: 1870 1943 2360 2390 2734 2978 1870
Size: 73 417 30 344 244 4545
Fragments arranged by size:
4545 417 344 244 73 30
BamHI G'GATC_C
Cuts at: 2983 3350 2983
Size: 367 5286
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2263 2317 2398 2659 2762
Size: 325 217 627 476 54 81 261 103
Cuts at: 2762 2974 4429 618
Size: 212 1455 1842
Fragments arranged by size:
1842 1455 627 476 325 261 217 212
103 81 54
BanII G_rGCy'C
Cuts at: 1391 2225 2921 2972 1391
Size: 834 696 51 4072
Fragments arranged by size:
4072 834 696 51
BbeI G_GCGC'C
Cuts at: 2321 2402 2663 2321
Size: 81 261 5311
Fragments arranged by size:
5311 261 81
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4682
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2086 2120 3844 3878 1903
Size: 34 149 34 1724 34 3678
Fragments arranged by size:
3678 1724 149 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3545 4755 3545
Size: 1210 4443
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5653
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2153 4264 136
Size: 107 122 71 631 605 481 2111 1525
Fragments arranged by size:
2111 1525 631 605 481 122 107 71
BglII A'GATC_T
Cuts at: 5648 5648
Size: 5653
BlpI GC'TnA_GC
Cuts at: 2129 3070 2129
Size: 941 4712
Bme1580I G_kGCm'C
Cuts at: 1790 2225 2268 3714 4870 1790
Size: 435 43 1446 1156 2573
Fragments arranged by size:
2573 1446 1156 435 43
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5653
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2063 2447 3384
Size: 416 53 83 399 24 646 384 937
Cuts at: 3384 4342 442
Size: 958 1753
Fragments arranged by size:
1753 958 937 646 416 399 384 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5653
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2454 2568 4313 1239
Size: 1215 114 1745 2579
Fragments arranged by size:
2579 1745 1215 114
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2649 3778 4643 4941 2649
Size: 1129 865 298 3361
Fragments arranged by size:
3361 1129 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4316 882
Size: 617 2817 2219
Fragments arranged by size:
2817 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3444 493
Size: 711 2240 2702
Fragments arranged by size:
2702 2240 711
BsaBI GATnn'nnATC
Cuts at: 5647 5647
Size: 5653
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2230 2318 2399 2438
Size: 53 83 186 936 697 88 81 39
Cuts at: 2438 2660 2749 3460 3842 4477 275
Size: 222 89 711 382 635 1451
Fragments arranged by size:
1451 936 711 697 635 382 222 186
89 88 83 81 53 39
BsaWI w'CCGG_w
Cuts at: 2494 2506 2518 4086 4484 4758 4905 5316 2494
Size: 12 12 1568 398 274 147 411 2831
Fragments arranged by size:
2831 1568 411 398 274 147 12 12
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5428
Fragments arranged by size:
5428 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2138 2408 1186
Size: 952 270 4431
Fragments arranged by size:
4431 952 270
BseYI C'CCAG_C
Cuts at: 2184 2602 4856 5352 2184
Size: 418 2254 496 2485
Fragments arranged by size:
2485 2254 496 418
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2309 2714 1163
Size: 573 573 405 4102
Fragments arranged by size:
4102 573 573 405
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2337 2370 2499 2511 2523 2529
Size: 275 186 336 33 129 12 12 6
Cuts at: 2529 2760 2772 3103 3864 4892 1540
Size: 231 12 331 761 1028 2301
Fragments arranged by size:
2301 1028 761 336 331 275 231 186
129 33 12 12 12 6
BsiHKAI G_wGCw'C
Cuts at: 3714 3799 4870 3714
Size: 85 1071 4497
Fragments arranged by size:
4497 1071 85
BsmI GAATG_Cn'
Cuts at: 3164 3257 3164
Size: 93 5560
BsmBI CGTCTCn'nnnn_
Cuts at: 2276 2276
Size: 5653
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2225 2268 2921 2972 3714 3799 4870
Size: 399 435 43 653 51 742 85 1071
Cuts at: 4870 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 653 435 399 85 51
43
BspEI T'CCGG_A
Cuts at: 5316 5316
Size: 5653
BspHI T'CATG_A
Cuts at: 3542 5272 3542
Size: 1730 3923
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2281 3008 844
Size: 1437 727 3489
Fragments arranged by size:
3489 1437 727
BsrBI CCG'CTC
Cuts at: 1394 1823 3540 1394
Size: 429 1717 3507
Fragments arranged by size:
3507 1717 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4148 4322 65
Size: 1183 2900 174 1396
Fragments arranged by size:
2900 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2110 2365 2759 4297 4484 1700
Size: 110 300 255 394 1538 187 2869
Fragments arranged by size:
2869 1538 394 300 255 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5653
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5653
BssSI C'ACGA_G
Cuts at: 2120 3713 4725 2120
Size: 1593 1012 3048
Fragments arranged by size:
3048 1593 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2209 794
Size: 1415 4238
BstBI TT'CG_AA
Cuts at: 4457 4457
Size: 5653
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5653
BstYI r'GATC_y
Cuts at: 1972 2983 3350 3735 3752 5204 5290 5302 5648
Size: 1011 367 385 17 1452 86 12 346
Cuts at: 5648 1972
Size: 1977
Fragments arranged by size:
1977 1452 1011 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2056 2321 2754 2766 513
Size: 552 991 265 433 12 3400
Fragments arranged by size:
3400 991 552 433 265 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2886 487
Size: 683 56 1660 3254
Fragments arranged by size:
3254 1660 683 56
BtsI GCAGTG_nn'
Cuts at: 2243 3169 3963 3983 2243
Size: 926 794 20 3913
Fragments arranged by size:
3913 926 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5653
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5618
DraI TTT'AAA
Cuts at: 2957 2957
Size: 5653
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4660 809
Size: 507 3344 1802
Fragments arranged by size:
3344 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2154 2367 2704
Size: 54 1425 50 275 18 324 213 337
Cuts at: 2704 2757 2769 3100 3989 5618 8
Size: 53 12 331 889 1629 43
Fragments arranged by size:
1629 1425 889 337 331 324 275 213
54 53 50 43 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 2367 2757 2769 3100 1537
Size: 275 555 390 12 331 4090
Fragments arranged by size:
4090 555 390 331 275 12
EarI CTCTTCn'nnn_
Cuts at: 1353 2108 3583 1353
Size: 755 1475 3423
Fragments arranged by size:
3423 1475 755
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4240 4614 4760 387
Size: 1064 138 2651 374 146 1280
Fragments arranged by size:
2651 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2454 2568 3716 4313 5100 5368 1239
Size: 393 822 114 1148 597 787 268 1524
Fragments arranged by size:
1524 1148 822 787 597 393 268 114
EcoNI CCTnn'n_nnAGG
Cuts at: 2393 2393
Size: 5653
EcoO109I rG'GnC_Cy
Cuts at: 2061 2221 2222 2061
Size: 160 1 5492
Fragments arranged by size:
5492 160 1
EcoRI G'AATT_C
Cuts at: 2962 3340 2962
Size: 378 5275
FspI TGC'GCA
Cuts at: 2081 4159 2081
Size: 2078 3575
FspAI rTGC'GCAy
Cuts at: 2081 2081
Size: 5653
HaeII r_GCGC'y
Cuts at: 1678 2091 2302 2321 2402 2663 2676 2781 2855
Size: 413 211 19 81 261 13 105 74
Cuts at: 2855 2904 4800 1678
Size: 49 1896 2531
Fragments arranged by size:
2531 1896 413 261 211 105 81 74
49 19 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2029 2061 2243 2275 2849
Size: 32 135 32 457 32 182 32 574
Cuts at: 2849 2881 4294 4326 4368 4400 1373
Size: 32 1413 32 42 32 2626
Fragments arranged by size:
2626 1413 574 457 182 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2997 3243 669
Size: 988 281 1059 246 3079
Fragments arranged by size:
3079 1059 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5653
HpaI GTT'AAC
Cuts at: 3243 3243
Size: 5653
KasI G'GCGC_C
Cuts at: 2317 2398 2659 2317
Size: 81 261 5311
Fragments arranged by size:
5311 261 81
KpnI G_GTAC'C
Cuts at: 2978 2978
Size: 5653
MfeI C'AATT_G
Cuts at: 3252 3252
Size: 5653
MluI A'CGCG_T
Cuts at: 2013 5529 2013
Size: 3516 2137
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2034 4767 4951 1305
Size: 729 2733 184 2007
Fragments arranged by size:
2733 2007 729 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3831
Fragments arranged by size:
3831 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3611 3970 4129 518
Size: 648 2445 359 159 2042
Fragments arranged by size:
2445 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2184 2215 2323 2372 2410 2728 2756 2768
Size: 578 31 108 49 38 318 28 12
Cuts at: 2768 3069 3746 4894 5139 1606
Size: 301 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 578 318 301 245 108
49 38 31 28 12
NaeI GCC'GGC
Cuts at: 2367 2761 2367
Size: 394 5259
NarI GG'CG_CC
Cuts at: 2318 2399 2660 2318
Size: 81 261 5311
Fragments arranged by size:
5311 261 81
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5101
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5653
NgoMIV G'CCGG_C
Cuts at: 2365 2759 2365
Size: 394 5259
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5653
NotI GC'GGCC_GC
Cuts at: 2769 3100 2769
Size: 331 5322
NspI r_CATG'y
Cuts at: 1132 1229 2212 3011 4556 1132
Size: 97 983 799 1545 2229
Fragments arranged by size:
2229 1545 983 799 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5653
PciI A'CATG_T
Cuts at: 4552 4552
Size: 5653
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5653
PmeI GTTT'AAAC
Cuts at: 2957 2957
Size: 5653
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3722 3754 5235 5267 3722
Size: 32 1481 32 4108
Fragments arranged by size:
4108 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2061 2061
Size: 5653
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5653
PsiI TTA'TAA
Cuts at: 3223 3223
Size: 5653
PspOMI G'GGCC_C
Cuts at: 2221 2221
Size: 5653
PspXI vC'TCGA_Gb
Cuts at: 2360 2390 2360
Size: 30 5623
PstI C_TGCA'G
Cuts at: 830 2145 2238 3005 830
Size: 1315 93 767 3478
Fragments arranged by size:
3478 1315 767 93
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5653
PvuII CAG'CTG
Cuts at: 2410 2728 2410
Size: 318 5335
SacII CC_GC'GG
Cuts at: 2324 2757 2769 2324
Size: 433 12 5208
Fragments arranged by size:
5208 433 12
SalI G'TCGA_C
Cuts at: 1936 2995 1936
Size: 1059 4594
SbfI CC_TGCA'GG
Cuts at: 3005 3005
Size: 5653
ScaI AGT'ACT
Cuts at: 2073 3901 5613 2073
Size: 1828 1712 2113
Fragments arranged by size:
2113 1828 1712
SfcI C'TryA_G
Cuts at: 826 1045 2141 2234 3001 4136 4817 5008 826
Size: 219 1096 93 767 1135 681 191 1471
Fragments arranged by size:
1471 1135 1096 767 681 219 191 93
SfiI GGCCn_nnn'nGGCC
Cuts at: 2153 2153
Size: 5653
SfoI GGC'GCC
Cuts at: 2319 2400 2661 2319
Size: 81 261 5311
Fragments arranged by size:
5311 261 81
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5653
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5653
SmaI CCC'GGG
Cuts at: 1872 2980 1872
Size: 1108 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2360 2390 2664 3757 4658 4920
Size: 197 926 417 30 274 1093 901 262
Cuts at: 4920 820
Size: 1553
Fragments arranged by size:
1553 1093 926 901 417 274 262 197
30
SnaBI TAC'GTA
Cuts at: 493 3444 493
Size: 2951 2702
SpeI A'CTAG_T
Cuts at: 152 2074 152
Size: 1922 3731
SphI G_CATG'C
Cuts at: 1132 3011 1132
Size: 1879 3774
SspI AAT'ATT
Cuts at: 5 52 3577 5
Size: 47 3525 2081
Fragments arranged by size:
3525 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5653
StyI C'CwwG_G
Cuts at: 513 1065 3092 513
Size: 552 2027 3074
Fragments arranged by size:
3074 2027 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2258 3680 3697 3850 4035 4480 325
Size: 1255 678 1422 17 153 185 445 1498
Fragments arranged by size:
1498 1422 1255 678 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2071 3899 5611
Size: 274 80 33 51 1443 94 1828 1712
Cuts at: 5611 96
Size: 138
Fragments arranged by size:
1828 1712 1443 274 138 94 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5613 5645 5613
Size: 32 5621
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5653
XbaI T'CTAG_A
Cuts at: 2989 2989
Size: 5653
XhoI C'TCGA_G
Cuts at: 1943 2360 2390 1943
Size: 417 30 5206
Fragments arranged by size:
5206 417 30
XmaI C'CCGG_G
Cuts at: 1870 2978 1870
Size: 1108 4545
XmnI GAAnn'nnTTC
Cuts at: 3782 3782
Size: 5653
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2750 3461 276
Size: 53 83 186 936 1216 711 2468
Fragments arranged by size:
2468 1216 936 711 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII SacII SalI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FseI NruI NsiI PacI PfoI PmlI PsrI
RsrII SacI SanDI SapI SexAI SrfI SwaI XcmI