Restriction map for
pFN21AB8943
(Circular) (Six-base) MAPSORT of: pFN21AB8943.seq Check: 1570 from: 1 to: 5608
pFN21AB8943 5608 bp
With 182 enzymes: SgfI *
December 8, 2014 13:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2438 2438
Size: 5608
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3418 278
Size: 53 83 186 936 1882 2468
Fragments arranged by size:
2468 1882 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2951 1937
Size: 1014 4594
Acc65I G'GTAC_C
Cuts at: 2929 2929
Size: 5608
AclI AA'CG_TT
Cuts at: 1563 3735 4108 1563
Size: 2172 373 3063
Fragments arranged by size:
3063 2172 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2356 3671 5055 5323 1632
Size: 724 1315 1384 268 1917
Fragments arranged by size:
1917 1384 1315 724 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5411
AflIII A'CryG_T
Cuts at: 1203 4507 5484 1203
Size: 3304 977 1327
Fragments arranged by size:
3304 1327 977
AgeI A'CCGG_T
Cuts at: 4439 4439
Size: 5608
AhdI GACnn_n'nnGTC
Cuts at: 4337 4337
Size: 5608
AleI CACnn'nnGTG
Cuts at: 1166 2387 1166
Size: 1221 4387
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2405 2427 1184
Size: 1221 22 4365
Fragments arranged by size:
4365 1221 22
AlwNI CAG_nnn'CTG
Cuts at: 2224 2379 2430 3351 4923 2224
Size: 155 51 921 1572 2909
Fragments arranged by size:
2909 1572 921 155 51
ApaI G_GGCC'C
Cuts at: 2131 2131
Size: 5608
ApaLI G'TGCA_C
Cuts at: 2402 2465 3665 4821 2402
Size: 63 1200 1156 3189
Fragments arranged by size:
3189 1200 1156 63
ApoI r'AATT_y
Cuts at: 1493 2917 3143 3295 5404 1493
Size: 1424 226 152 2109 1697
Fragments arranged by size:
2109 1697 1424 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4162 160
Size: 872 3130 1606
Fragments arranged by size:
3130 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5608
AvaI C'yCGr_G
Cuts at: 1870 1943 2029 2045 2122 2695 2933 1870
Size: 73 86 16 77 573 238 4545
Fragments arranged by size:
4545 573 238 86 77 73 16
BamHI G'GATC_C
Cuts at: 2938 3305 2938
Size: 367 5241
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2734 2929 4384 618
Size: 325 217 627 947 195 1455 1842
Fragments arranged by size:
1842 1455 947 627 325 217 195
BanII G_rGCy'C
Cuts at: 1391 2019 2121 2131 2823 2927 1391
Size: 628 102 10 692 104 4072
Fragments arranged by size:
4072 692 628 104 102 10
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2752 928
Size: 971 853 3784
Fragments arranged by size:
3784 971 853
BbvCI CC'TCA_GC
Cuts at: 2571 2571
Size: 5608
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3799 3833 1903
Size: 34 50 34 1778 34 3678
Fragments arranged by size:
3678 1778 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3500 4710 3500
Size: 1210 4398
BclI T'GATC_A
Cuts at: 1548 2421 1548
Size: 873 4735
BfrBI ATG'CAT
Cuts at: 2008 2008
Size: 5608
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2309 4219 136
Size: 107 122 71 631 605 637 1910 1525
Fragments arranged by size:
1910 1525 637 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5603 5603
Size: 5608
BlpI GC'TnA_GC
Cuts at: 3025 3025
Size: 5608
Bme1580I G_kGCm'C
Cuts at: 1790 2131 2406 2469 2739 3669 4825 1790
Size: 341 275 63 270 930 1156 2573
Fragments arranged by size:
2573 1156 930 341 275 270 63
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5608
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3339 4297 442
Size: 416 53 83 399 24 1922 958 1753
Fragments arranged by size:
1922 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5608
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2254 4268 1239
Size: 1015 2014 2579
Fragments arranged by size:
2579 2014 1015
Bpu10I CC'TnA_GC
Cuts at: 2571 2571
Size: 5608
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2266 2594 3733 4598 4896 2266
Size: 328 1139 865 298 2978
Fragments arranged by size:
2978 1139 865 328 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4271 882
Size: 617 2772 2219
Fragments arranged by size:
2772 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3399 493
Size: 711 2195 2702
Fragments arranged by size:
2702 2195 711
BsaBI GATnn'nnATC
Cuts at: 2753 5602 2753
Size: 2849 2759
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2888 3415 3797 4432
Size: 53 83 186 936 1355 527 382 635
Cuts at: 4432 275
Size: 1451
Fragments arranged by size:
1451 1355 936 635 527 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2011 4041 4439 4713 4860 5271 2011
Size: 2030 398 274 147 411 2348
Fragments arranged by size:
2348 2030 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2195 2225 1178
Size: 30 165 30 792 30 4561
Fragments arranged by size:
4561 792 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5608
BseYI C'CCAG_C
Cuts at: 2589 4811 5307 2589
Size: 2222 496 2890
Fragments arranged by size:
2890 2222 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2345 2365 1163
Size: 573 609 20 4406
Fragments arranged by size:
4406 609 573 20
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2806 3058 3819 4847 1540
Size: 275 186 805 252 761 1028 2301
Fragments arranged by size:
2301 1028 805 761 275 252 186
BsiHKAI G_wGCw'C
Cuts at: 2211 2406 2469 2823 3669 3754 4825 2211
Size: 195 63 354 846 85 1071 2994
Fragments arranged by size:
2994 1071 846 354 195 85 63
BsmI GAATG_Cn'
Cuts at: 3119 3212 3119
Size: 93 5515
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2019 2121 2131 2211 2406 2469 2739
Size: 399 229 102 10 80 195 63 270
Cuts at: 2739 2823 2927 3669 3754 4825 1391
Size: 84 104 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 270 229 195 104
102 85 84 80 63 10
BspEI T'CCGG_A
Cuts at: 2011 5271 2011
Size: 3260 2348
BspHI T'CATG_A
Cuts at: 2418 3497 5227 2418
Size: 1079 1730 2799
Fragments arranged by size:
2799 1730 1079
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2438 2963 844
Size: 1594 525 3489
Fragments arranged by size:
3489 1594 525
BsrBI CCG'CTC
Cuts at: 1394 1823 2781 3495 1394
Size: 429 958 714 3507
Fragments arranged by size:
3507 958 714 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2319 4103 4277 65
Size: 1183 1071 1784 174 1396
Fragments arranged by size:
1784 1396 1183 1071 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4252 4439 1700
Size: 110 2442 187 2869
Fragments arranged by size:
2869 2442 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5608
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5608
BssSI C'ACGA_G
Cuts at: 3668 4680 3668
Size: 1012 4596
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2215 794
Size: 1421 4187
BstBI TT'CG_AA
Cuts at: 4412 4412
Size: 5608
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5608
BstXI CCAn_nnnn'nTGG
Cuts at: 2392 2731 2392
Size: 339 5269
BstYI r'GATC_y
Cuts at: 1972 2938 3305 3690 3707 5159 5245 5257 5603
Size: 966 367 385 17 1452 86 12 346
Cuts at: 5603 1972
Size: 1977
Fragments arranged by size:
1977 1452 966 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2304 513
Size: 552 1239 3817
Fragments arranged by size:
3817 1239 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2350 2698 487
Size: 683 56 1124 348 3397
Fragments arranged by size:
3397 1124 683 348 56
BtsI GCAGTG_nn'
Cuts at: 3124 3918 3938 3124
Size: 794 20 4794
Fragments arranged by size:
4794 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5608
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5573
DraI TTT'AAA
Cuts at: 2912 2912
Size: 5608
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4615 809
Size: 507 3299 1802
Fragments arranged by size:
3299 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2173 3055 3944
Size: 54 1425 50 275 18 343 882 889
Cuts at: 3944 5573 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 882 343 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3055 1537
Size: 275 1243 4090
Fragments arranged by size:
4090 1243 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2567 3538 1353
Size: 1214 971 3423
Fragments arranged by size:
3423 1214 971
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2137 2849 4195 4569 4715 387
Size: 1064 138 548 712 1346 374 146 1280
Fragments arranged by size:
1346 1280 1064 712 548 374 146 138
EcoICRI GAG'CTC
Cuts at: 2821 2821
Size: 5608
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2254 2356 3671 4268 5055 5323 1239
Size: 393 622 102 1315 597 787 268 1524
Fragments arranged by size:
1524 1315 787 622 597 393 268 102
EcoNI CCTnn'n_nnAGG
Cuts at: 2570 2570
Size: 5608
EcoO109I rG'GnC_Cy
Cuts at: 2127 2128 2127
Size: 1 5607
EcoRI G'AATT_C
Cuts at: 2917 3295 2917
Size: 378 5230
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2260 2260
Size: 5608
FspI TGC'GCA
Cuts at: 4114 4114
Size: 5608
HaeII r_GCGC'y
Cuts at: 1678 2225 4755 1678
Size: 547 2530 2531
Fragments arranged by size:
2531 2530 547
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2269 2301 4249 4281 4323
Size: 32 135 32 697 32 1948 32 42
Cuts at: 4323 4355 1373
Size: 32 2626
Fragments arranged by size:
2626 1948 697 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2952 3198 669
Size: 988 281 1014 246 3079
Fragments arranged by size:
3079 1014 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2258 748
Size: 1510 4098
HpaI GTT'AAC
Cuts at: 3198 3198
Size: 5608
KpnI G_GTAC'C
Cuts at: 2933 2933
Size: 5608
MfeI C'AATT_G
Cuts at: 3207 3207
Size: 5608
MluI A'CGCG_T
Cuts at: 5484 5484
Size: 5608
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4722 4906 1305
Size: 3417 184 2007
Fragments arranged by size:
3417 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3786
Fragments arranged by size:
3786 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2387 2729 2732 3566 3925 4084 518
Size: 648 1221 342 3 834 359 159 2042
Fragments arranged by size:
2042 1221 834 648 359 342 159 3
MspA1I CmG'CkG
Cuts at: 1606 2079 2106 2329 2685 2708 2860 3024 3701
Size: 473 27 223 356 23 152 164 677
Cuts at: 3701 4849 5094 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 473 356 245 223 164
152 27 23
NcoI C'CATG_G
Cuts at: 513 1065 2304 513
Size: 552 1239 3817
Fragments arranged by size:
3817 1239 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5608
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5608
NotI GC'GGCC_GC
Cuts at: 3055 3055
Size: 5608
NsiI A_TGCA'T
Cuts at: 2010 2010
Size: 5608
NspI r_CATG'y
Cuts at: 1132 1229 2509 2966 4511 1132
Size: 97 1280 457 1545 2229
Fragments arranged by size:
2229 1545 1280 457 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5608
PciI A'CATG_T
Cuts at: 4507 4507
Size: 5608
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5608
PfoI T'CCnGG_A
Cuts at: 2814 2814
Size: 5608
PmeI GTTT'AAAC
Cuts at: 2912 2912
Size: 5608
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3677 3709 5190 5222 3677
Size: 32 1481 32 4063
Fragments arranged by size:
4063 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5608
PsiI TTA'TAA
Cuts at: 3178 3178
Size: 5608
PspOMI G'GGCC_C
Cuts at: 2127 2127
Size: 5608
PstI C_TGCA'G
Cuts at: 830 2960 830
Size: 2130 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5608
PvuII CAG'CTG
Cuts at: 2329 2708 2860 2329
Size: 379 152 5077
Fragments arranged by size:
5077 379 152
SacI G_AGCT'C
Cuts at: 2823 2823
Size: 5608
SalI G'TCGA_C
Cuts at: 1936 2950 1936
Size: 1014 4594
SapI GCTCTTCn'nnn_
Cuts at: 2567 2567
Size: 5608
SbfI CC_TGCA'GG
Cuts at: 2960 2960
Size: 5608
ScaI AGT'ACT
Cuts at: 3856 5568 3856
Size: 1712 3896
SfcI C'TryA_G
Cuts at: 826 1045 2956 4091 4772 4963 826
Size: 219 1911 1135 681 191 1471
Fragments arranged by size:
1911 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5608
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5608
SmaI CCC'GGG
Cuts at: 1872 2124 2697 2935 1872
Size: 252 573 238 4545
Fragments arranged by size:
4545 573 252 238
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2245 2609 3712 4613 4875 820
Size: 197 926 302 364 1103 901 262 1553
Fragments arranged by size:
1553 1103 926 901 364 302 262 197
SnaBI TAC'GTA
Cuts at: 493 3399 493
Size: 2906 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5608
SphI G_CATG'C
Cuts at: 1132 2509 2966 1132
Size: 1377 457 3774
Fragments arranged by size:
3774 1377 457
SspI AAT'ATT
Cuts at: 5 52 3532 5
Size: 47 3480 2081
Fragments arranged by size:
3480 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5608
StyI C'CwwG_G
Cuts at: 513 1065 2304 2630 3047 513
Size: 552 1239 326 417 3074
Fragments arranged by size:
3074 1239 552 417 326
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2470 2816 2820 3635 3652 3805 3990
Size: 1255 890 346 4 815 17 153 185
Cuts at: 3990 4435 325
Size: 445 1498
Fragments arranged by size:
1498 1255 890 815 445 346 185 153
17 4
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3854 5566 96
Size: 274 80 33 51 1443 1877 1712 138
Fragments arranged by size:
1877 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5568 5600 5568
Size: 32 5576
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5608
XbaI T'CTAG_A
Cuts at: 2944 2944
Size: 5608
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2311 2392 2311
Size: 81 5527
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5608
XmaI C'CCGG_G
Cuts at: 1870 2122 2695 2933 1870
Size: 252 573 238 4545
Fragments arranged by size:
4545 573 252 238
XmnI GAAnn'nnTTC
Cuts at: 3737 3737
Size: 5608
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3416 276
Size: 53 83 186 936 1882 2468
Fragments arranged by size:
2468 1882 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspXI
PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI
SwaI