Restriction map for
pFN21AB8942
(Circular) (Six-base) MAPSORT of: pFN21AB8942.seq Check: 9183 from: 1 to: 5608
pFN21AB8942 5608 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2422 2695 2786 2422
Size: 273 91 5244
Fragments arranged by size:
5244 273 91
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3418 278
Size: 53 83 186 936 1882 2468
Fragments arranged by size:
2468 1882 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2951 1937
Size: 1014 4594
Acc65I G'GTAC_C
Cuts at: 2469 2929 2469
Size: 460 5148
AclI AA'CG_TT
Cuts at: 1563 3735 4108 1563
Size: 2172 373 3063
Fragments arranged by size:
3063 2172 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3671 5055 5323 1632
Size: 2039 1384 268 1917
Fragments arranged by size:
2039 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5411
AflIII A'CryG_T
Cuts at: 1203 4507 5484 1203
Size: 3304 977 1327
Fragments arranged by size:
3304 1327 977
AgeI A'CCGG_T
Cuts at: 4439 4439
Size: 5608
AhdI GACnn_n'nnGTC
Cuts at: 4337 4337
Size: 5608
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5608
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5608
AlwNI CAG_nnn'CTG
Cuts at: 2134 2437 2904 3351 4923 2134
Size: 303 467 447 1572 2819
Fragments arranged by size:
2819 1572 467 447 303
ApaI G_GGCC'C
Cuts at: 2672 2679 2672
Size: 7 5601
ApaLI G'TGCA_C
Cuts at: 3665 4821 3665
Size: 1156 4452
ApoI r'AATT_y
Cuts at: 1493 2917 3143 3295 5404 1493
Size: 1424 226 152 2109 1697
Fragments arranged by size:
2109 1697 1424 226 152
AscI GG'CGCG_CC
Cuts at: 2790 2790
Size: 5608
AseI AT'TA_AT
Cuts at: 160 1032 4162 160
Size: 872 3130 1606
Fragments arranged by size:
3130 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5608
AvaI C'yCGr_G
Cuts at: 1870 1943 2483 2672 2797 2933 1870
Size: 73 540 189 125 136 4545
Fragments arranged by size:
4545 540 189 136 125 73
BamHI G'GATC_C
Cuts at: 2938 3305 2938
Size: 367 5241
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2323 2469 2774 2929 4384
Size: 325 217 627 536 146 305 155 1455
Cuts at: 4384 618
Size: 1842
Fragments arranged by size:
1842 1455 627 536 325 305 217 155
146
BanII G_rGCy'C
Cuts at: 1391 2018 2117 2672 2679 2927 1391
Size: 627 99 555 7 248 4072
Fragments arranged by size:
4072 627 555 248 99 7
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4637
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2700 2734 3799 3833 1903
Size: 34 50 34 679 34 1065 34 3678
Fragments arranged by size:
3678 1065 679 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3500 4710 3500
Size: 1210 4398
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5608
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4219 136
Size: 107 122 71 631 605 2547 1525
Fragments arranged by size:
2547 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2535 5603 2535
Size: 3068 2540
BlpI GC'TnA_GC
Cuts at: 2012 3025 2012
Size: 1013 4595
Bme1580I G_kGCm'C
Cuts at: 1790 2637 2672 2679 3669 4825 1790
Size: 847 35 7 990 1156 2573
Fragments arranged by size:
2573 1156 990 847 35 7
BmgBI CAC'GTC
Cuts at: 1318 2520 1318
Size: 1202 4406
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3339 4297 442
Size: 416 53 83 399 24 1922 958 1753
Fragments arranged by size:
1922 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5608
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2081 2465 4268 1239
Size: 842 384 1803 2579
Fragments arranged by size:
2579 1803 842 384
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3733 4598 4896 3733
Size: 865 298 4445
Fragments arranged by size:
4445 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4271 882
Size: 617 2772 2219
Fragments arranged by size:
2772 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3399 493
Size: 711 2195 2702
Fragments arranged by size:
2702 2195 711
BsaBI GATnn'nnATC
Cuts at: 5602 5602
Size: 5608
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3415 3797 4432 275
Size: 53 83 186 936 1882 382 635 1451
Fragments arranged by size:
1882 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4041 4439 4713 4860 5271 4041
Size: 398 274 147 411 4378
Fragments arranged by size:
4378 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5383
Fragments arranged by size:
5383 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5608
BseYI C'CCAG_C
Cuts at: 2696 4811 5307 2696
Size: 2115 496 2997
Fragments arranged by size:
2997 2115 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2730 1163
Size: 573 994 4041
Fragments arranged by size:
4041 994 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3058 3819 4847 1540
Size: 275 186 1057 761 1028 2301
Fragments arranged by size:
2301 1057 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2418 2496 2755 3669 3754 4825 2418
Size: 78 259 914 85 1071 3201
Fragments arranged by size:
3201 1071 914 259 85 78
BsmI GAATG_Cn'
Cuts at: 2860 3119 3212 2860
Size: 259 93 5256
Fragments arranged by size:
5256 259 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2018 2117 2418 2496 2637 2672 2679
Size: 399 228 99 301 78 141 35 7
Cuts at: 2679 2755 2927 3669 3754 4825 1391
Size: 76 172 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 301 228 172 141
99 85 78 76 35 7
BspEI T'CCGG_A
Cuts at: 5271 5271
Size: 5608
BspHI T'CATG_A
Cuts at: 3497 5227 3497
Size: 1730 3878
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2422 2518 2695 2786 2963 844
Size: 1578 96 177 91 177 3489
Fragments arranged by size:
3489 1578 177 177 96 91
BsrBI CCG'CTC
Cuts at: 1394 1823 2071 2373 2449 3495 1394
Size: 429 248 302 76 1046 3507
Fragments arranged by size:
3507 1046 429 302 248 76
BsrDI GCAATG_nn'
Cuts at: 65 1248 4103 4277 65
Size: 1183 2855 174 1396
Fragments arranged by size:
2855 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4252 4439 1700
Size: 110 2442 187 2869
Fragments arranged by size:
2869 2442 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5608
BssHII G'CGCG_C
Cuts at: 1925 2505 2790 1925
Size: 580 285 4743
Fragments arranged by size:
4743 580 285
BssSI C'ACGA_G
Cuts at: 3668 4680 3668
Size: 1012 4596
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2661 794
Size: 1867 3741
BstBI TT'CG_AA
Cuts at: 4412 4412
Size: 5608
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5608
BstYI r'GATC_y
Cuts at: 1972 2535 2938 3305 3690 3707 5159 5245 5257
Size: 563 403 367 385 17 1452 86 12
Cuts at: 5257 5603 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 563 403 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2082 2099 513
Size: 552 1017 17 4022
Fragments arranged by size:
4022 1017 552 17
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2664 487
Size: 683 56 1438 3431
Fragments arranged by size:
3431 1438 683 56
BtsI GCAGTG_nn'
Cuts at: 2721 3124 3918 3938 2721
Size: 403 794 20 4391
Fragments arranged by size:
4391 794 403 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5608
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2161 2196 529
Size: 35 1597 35 3941
Fragments arranged by size:
3941 1597 35 35
DraI TTT'AAA
Cuts at: 2912 2912
Size: 5608
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4615 809
Size: 507 3299 1802
Fragments arranged by size:
3299 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2368 2499 2602
Size: 54 1425 50 275 18 538 131 103
Cuts at: 2602 2823 2848 3055 3944 5573 8
Size: 221 25 207 889 1629 43
Fragments arranged by size:
1629 1425 889 538 275 221 207 131
103 54 50 43 25 18
EagI C'GGCC_G
Cuts at: 1537 1812 3055 1537
Size: 275 1243 4090
Fragments arranged by size:
4090 1243 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2105 3538 1353
Size: 752 1433 3423
Fragments arranged by size:
3423 1433 752
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4195 4569 4715 387
Size: 1064 138 2606 374 146 1280
Fragments arranged by size:
2606 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2081 2465 3671 4268 5055 5323 1239
Size: 393 449 384 1206 597 787 268 1524
Fragments arranged by size:
1524 1206 787 597 449 393 384 268
EcoO109I rG'GnC_Cy
Cuts at: 2228 2669 2801 2901 2228
Size: 441 132 100 4935
Fragments arranged by size:
4935 441 132 100
EcoRI G'AATT_C
Cuts at: 2917 3295 2917
Size: 378 5230
FspI TGC'GCA
Cuts at: 4114 4114
Size: 5608
HaeII r_GCGC'y
Cuts at: 1678 2455 2483 2578 4755 1678
Size: 777 28 95 2177 2531
Fragments arranged by size:
2531 2177 777 95 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4249 4281 4323 4355 1373
Size: 32 135 32 2677 32 42 32 2626
Fragments arranged by size:
2677 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2952 3198 669
Size: 988 281 1014 246 3079
Fragments arranged by size:
3079 1014 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5608
HpaI GTT'AAC
Cuts at: 3198 3198
Size: 5608
KpnI G_GTAC'C
Cuts at: 2473 2933 2473
Size: 460 5148
MfeI C'AATT_G
Cuts at: 3207 3207
Size: 5608
MluI A'CGCG_T
Cuts at: 5484 5484
Size: 5608
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4722 4906 1305
Size: 3417 184 2007
Fragments arranged by size:
3417 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2501 2604 2825 2850 10
Size: 54 1425 343 669 103 221 25 2768
Fragments arranged by size:
2768 1425 669 343 221 103 54 25
MslI CAynn'nnrTG
Cuts at: 518 1166 3566 3925 4084 518
Size: 648 2400 359 159 2042
Fragments arranged by size:
2400 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2084 2101 2349 2467 2748 3024 3701 4849
Size: 478 17 248 118 281 276 677 1148
Cuts at: 4849 5094 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 478 281 276 248 245
118 17
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5056
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5608
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5608
NotI GC'GGCC_GC
Cuts at: 3055 3055
Size: 5608
NspI r_CATG'y
Cuts at: 1132 1229 2966 4511 1132
Size: 97 1737 1545 2229
Fragments arranged by size:
2229 1737 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5608
PciI A'CATG_T
Cuts at: 4507 4507
Size: 5608
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5608
PmeI GTTT'AAAC
Cuts at: 2912 2912
Size: 5608
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2282 2314 3677 3709 5190 5222 2282
Size: 32 1363 32 1481 32 2668
Fragments arranged by size:
2668 1481 1363 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2228 2801 2228
Size: 573 5035
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5608
PsiI TTA'TAA
Cuts at: 3178 3178
Size: 5608
PspOMI G'GGCC_C
Cuts at: 2668 2675 2668
Size: 7 5601
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2540 2572 2540
Size: 32 5576
PstI C_TGCA'G
Cuts at: 830 2514 2892 2960 830
Size: 1684 378 68 3478
Fragments arranged by size:
3478 1684 378 68
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5608
SacII CC_GC'GG
Cuts at: 2085 2102 2085
Size: 17 5591
SalI G'TCGA_C
Cuts at: 1936 2950 1936
Size: 1014 4594
SanDI GG'GwC_CC
Cuts at: 2228 2228
Size: 5608
SapI GCTCTTCn'nnn_
Cuts at: 2105 2105
Size: 5608
SbfI CC_TGCA'GG
Cuts at: 2960 2960
Size: 5608
ScaI AGT'ACT
Cuts at: 3856 5568 3856
Size: 1712 3896
SfcI C'TryA_G
Cuts at: 826 1045 2510 2704 2743 2888 2956 4091 4772
Size: 219 1465 194 39 145 68 1135 681
Cuts at: 4772 4963 826
Size: 191 1471
Fragments arranged by size:
1471 1465 1135 681 219 194 191 145
68 39
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5608
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5608
SmaI CCC'GGG
Cuts at: 1872 2674 2935 1872
Size: 802 261 4545
Fragments arranged by size:
4545 802 261
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3712 4613 4875 820
Size: 197 926 1769 901 262 1553
Fragments arranged by size:
1769 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3399 493
Size: 2906 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5608
SphI G_CATG'C
Cuts at: 1132 2966 1132
Size: 1834 3774
SspI AAT'ATT
Cuts at: 5 52 3532 5
Size: 47 3480 2081
Fragments arranged by size:
3480 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5608
StyI C'CwwG_G
Cuts at: 513 1065 2385 3047 513
Size: 552 1320 662 3074
Fragments arranged by size:
3074 1320 662 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2029 3635 3652 3805 3990 4435 325
Size: 1255 449 1606 17 153 185 445 1498
Fragments arranged by size:
1606 1498 1255 449 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3854 5566 96
Size: 274 80 33 51 1443 1877 1712 138
Fragments arranged by size:
1877 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2676 2708 5568 5600 2676
Size: 32 2860 32 2684
Fragments arranged by size:
2860 2684 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5608
XbaI T'CTAG_A
Cuts at: 2944 2944
Size: 5608
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5608
XmaI C'CCGG_G
Cuts at: 1870 2672 2933 1870
Size: 802 261 4545
Fragments arranged by size:
4545 802 261
XmnI GAAnn'nnTTC
Cuts at: 2124 3737 2124
Size: 1613 3995
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3416 276
Size: 53 83 186 936 1882 2468
Fragments arranged by size:
2468 1882 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI
PstI PvuI SacII SalI SanDI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AvrII BaeI BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspXI PvuII
RsrII SacI SexAI SfiI SfoI SrfI SwaI XcmI