Restriction map for
pFN21AB8938
(Circular) (Six-base) MAPSORT of: pFN21AB8938.seq Check: 537 from: 1 to: 5575
pFN21AB8938 5575 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3385 278
Size: 53 83 186 936 1849 2468
Fragments arranged by size:
2468 1849 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2593 2918 1937
Size: 656 325 4594
Fragments arranged by size:
4594 656 325
Acc65I G'GTAC_C
Cuts at: 2896 2896
Size: 5575
AclI AA'CG_TT
Cuts at: 1563 3702 4075 1563
Size: 2139 373 3063
Fragments arranged by size:
3063 2139 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3638 5022 5290 1632
Size: 2006 1384 268 1917
Fragments arranged by size:
2006 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5378
AflIII A'CryG_T
Cuts at: 1203 4474 5451 1203
Size: 3271 977 1327
Fragments arranged by size:
3271 1327 977
AgeI A'CCGG_T
Cuts at: 4406 4406
Size: 5575
AhdI GACnn_n'nnGTC
Cuts at: 4304 4304
Size: 5575
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5575
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5575
AlwNI CAG_nnn'CTG
Cuts at: 2588 3318 4890 2588
Size: 730 1572 3273
Fragments arranged by size:
3273 1572 730
ApaLI G'TGCA_C
Cuts at: 3632 4788 3632
Size: 1156 4419
ApoI r'AATT_y
Cuts at: 1493 2648 2783 2884 3110 3262 5371 1493
Size: 1155 135 101 226 152 2109 1697
Fragments arranged by size:
2109 1697 1155 226 152 135 101
AseI AT'TA_AT
Cuts at: 160 1032 4129 160
Size: 872 3097 1606
Fragments arranged by size:
3097 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5575
AvaI C'yCGr_G
Cuts at: 1870 1943 2117 2900 1870
Size: 73 174 783 4545
Fragments arranged by size:
4545 783 174 73
BamHI G'GATC_C
Cuts at: 2905 3272 2905
Size: 367 5208
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2896 4351 618
Size: 325 217 627 1109 1455 1842
Fragments arranged by size:
1842 1455 1109 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2403 2543 2894 1391
Size: 1012 140 351 4072
Fragments arranged by size:
4072 1012 351 140
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2611 2856 928
Size: 971 712 245 3647
Fragments arranged by size:
3647 971 712 245
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3766 3800 1903
Size: 34 1829 34 3678
Fragments arranged by size:
3678 1829 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2411 3467 4677 2411
Size: 1056 1210 3309
Fragments arranged by size:
3309 1210 1056
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5575
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2321 4186 136
Size: 107 122 71 631 605 649 1865 1525
Fragments arranged by size:
1865 1525 649 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5570 5570
Size: 5575
BlpI GC'TnA_GC
Cuts at: 2992 2992
Size: 5575
Bme1580I G_kGCm'C
Cuts at: 1790 3636 4792 1790
Size: 1846 1156 2573
Fragments arranged by size:
2573 1846 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5575
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2256 3306 4264
Size: 416 53 83 399 24 839 1050 958
Cuts at: 4264 442
Size: 1753
Fragments arranged by size:
1753 1050 958 839 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5575
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2562 2562
Size: 5575
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2864 4235 1239
Size: 1625 1371 2579
Fragments arranged by size:
2579 1625 1371
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3700 4565 4863 3700
Size: 865 298 4412
Fragments arranged by size:
4412 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2566 4238 882
Size: 617 1067 1672 2219
Fragments arranged by size:
2219 1672 1067 617
BsaAI yAC'GTr
Cuts at: 493 1204 3366 493
Size: 711 2162 2702
Fragments arranged by size:
2702 2162 711
BsaBI GATnn'nnATC
Cuts at: 5569 5569
Size: 5575
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3382 3764 4399 275
Size: 53 83 186 936 1849 382 635 1451
Fragments arranged by size:
1849 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4008 4406 4680 4827 5238 4008
Size: 398 274 147 411 4345
Fragments arranged by size:
4345 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5350
Fragments arranged by size:
5350 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2325 2328 1186
Size: 1139 3 4433
Fragments arranged by size:
4433 1139 3
BseYI C'CCAG_C
Cuts at: 2454 4778 5274 2454
Size: 2324 496 2755
Fragments arranged by size:
2755 2324 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5002
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2131 2310 3025 3786 4814 1540
Size: 275 186 130 179 715 761 1028 2301
Fragments arranged by size:
2301 1028 761 715 275 186 179 130
BsiHKAI G_wGCw'C
Cuts at: 2543 3636 3721 4792 2543
Size: 1093 85 1071 3326
Fragments arranged by size:
3326 1093 1071 85
BsmI GAATG_Cn'
Cuts at: 3086 3179 3086
Size: 93 5482
BsmBI CGTCTCn'nnnn_
Cuts at: 2550 2550
Size: 5575
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2403 2543 2894 3636 3721 4792 1391
Size: 399 613 140 351 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 613 399 351 140 85
BspEI T'CCGG_A
Cuts at: 5238 5238
Size: 5575
BspHI T'CATG_A
Cuts at: 3464 5194 3464
Size: 1730 3845
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2930 844
Size: 2086 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3462 1394
Size: 429 1639 3507
Fragments arranged by size:
3507 1639 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4070 4244 65
Size: 1183 2822 174 1396
Fragments arranged by size:
2822 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2180 4219 4406 1700
Size: 110 370 2039 187 2869
Fragments arranged by size:
2869 2039 370 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5575
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5575
BssSI C'ACGA_G
Cuts at: 3635 4647 3635
Size: 1012 4563
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5575
BstBI TT'CG_AA
Cuts at: 4379 4379
Size: 5575
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5575
BstYI r'GATC_y
Cuts at: 1972 2905 3272 3657 3674 5126 5212 5224 5570
Size: 933 367 385 17 1452 86 12 346
Cuts at: 5570 1972
Size: 1977
Fragments arranged by size:
1977 1452 933 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2564 513
Size: 552 1499 3524
Fragments arranged by size:
3524 1499 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4836
Fragments arranged by size:
4836 683 56
BtsI GCAGTG_nn'
Cuts at: 3091 3885 3905 3091
Size: 794 20 4761
Fragments arranged by size:
4761 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5575
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5540
DraI TTT'AAA
Cuts at: 2879 2879
Size: 5575
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4582 809
Size: 507 3266 1802
Fragments arranged by size:
3266 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2128 2307 3022
Size: 54 1425 50 275 18 298 179 715
Cuts at: 3022 3911 5540 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 715 298 275 179 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2128 2307 3022 1537
Size: 275 316 179 715 4090
Fragments arranged by size:
4090 715 316 275 179
EarI CTCTTCn'nnn_
Cuts at: 1353 2593 3505 1353
Size: 1240 912 3423
Fragments arranged by size:
3423 1240 912
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2340 2346 4162 4536 4682 387
Size: 1064 138 751 6 1816 374 146 1280
Fragments arranged by size:
1816 1280 1064 751 374 146 138 6
EcoICRI GAG'CTC
Cuts at: 2541 2541
Size: 5575
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2864 3638 4235 5022 5290 1239
Size: 393 1232 774 597 787 268 1524
Fragments arranged by size:
1524 1232 787 774 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2518 2518
Size: 5575
EcoRI G'AATT_C
Cuts at: 2884 3262 2884
Size: 378 5197
FspI TGC'GCA
Cuts at: 4081 4081
Size: 5575
HaeII r_GCGC'y
Cuts at: 1678 2178 4722 1678
Size: 500 2544 2531
Fragments arranged by size:
2544 2531 500
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2374 2406 2477 2509 4216
Size: 32 135 32 802 32 71 32 1707
Cuts at: 4216 4248 4290 4322 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1707 802 135 71 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2919 3165 669
Size: 988 281 981 246 3079
Fragments arranged by size:
3079 988 981 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5575
HpaI GTT'AAC
Cuts at: 3165 3165
Size: 5575
KpnI G_GTAC'C
Cuts at: 2900 2900
Size: 5575
MfeI C'AATT_G
Cuts at: 3174 3174
Size: 5575
MluI A'CGCG_T
Cuts at: 5451 5451
Size: 5575
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2619 4689 4873 1305
Size: 1314 2070 184 2007
Fragments arranged by size:
2070 2007 1314 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3753
Fragments arranged by size:
3753 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3533 3892 4051 518
Size: 648 2367 359 159 2042
Fragments arranged by size:
2367 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2029 2312 2991 3668 4816 5061 1606
Size: 423 283 679 677 1148 245 2120
Fragments arranged by size:
2120 1148 679 677 423 283 245
NaeI GCC'GGC
Cuts at: 2182 2182
Size: 5575
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5023
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5575
NgoMIV G'CCGG_C
Cuts at: 2180 2180
Size: 5575
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5575
NotI GC'GGCC_GC
Cuts at: 3022 3022
Size: 5575
NspI r_CATG'y
Cuts at: 1132 1229 2933 4478 1132
Size: 97 1704 1545 2229
Fragments arranged by size:
2229 1704 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5575
PciI A'CATG_T
Cuts at: 4474 4474
Size: 5575
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2842 1108
Size: 1734 3841
PfoI T'CCnGG_A
Cuts at: 2240 2240
Size: 5575
PmeI GTTT'AAAC
Cuts at: 2879 2879
Size: 5575
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3644 3676 5157 5189 3644
Size: 32 1481 32 4030
Fragments arranged by size:
4030 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2518 2518
Size: 5575
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5575
PsiI TTA'TAA
Cuts at: 3145 3145
Size: 5575
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2015 2047 2015
Size: 32 5543
PstI C_TGCA'G
Cuts at: 830 2160 2927 830
Size: 1330 767 3478
Fragments arranged by size:
3478 1330 767
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5575
SacI G_AGCT'C
Cuts at: 2543 2543
Size: 5575
SalI G'TCGA_C
Cuts at: 1936 2917 1936
Size: 981 4594
SbfI CC_TGCA'GG
Cuts at: 2927 2927
Size: 5575
ScaI AGT'ACT
Cuts at: 2282 3823 5535 2282
Size: 1541 1712 2322
Fragments arranged by size:
2322 1712 1541
SfcI C'TryA_G
Cuts at: 826 1045 2156 2614 2923 4058 4739 4930 826
Size: 219 1111 458 309 1135 681 191 1471
Fragments arranged by size:
1471 1135 1111 681 458 309 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5575
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5575
SmaI CCC'GGG
Cuts at: 1872 2902 1872
Size: 1030 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3679 4580 4842 820
Size: 197 926 1736 901 262 1553
Fragments arranged by size:
1736 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3366 493
Size: 2873 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5575
SphI G_CATG'C
Cuts at: 1132 2933 1132
Size: 1801 3774
SspI AAT'ATT
Cuts at: 5 52 2509 3499 5
Size: 47 2457 990 2081
Fragments arranged by size:
2457 2081 990 47
StuI AGG'CCT
Cuts at: 1517 2865 1517
Size: 1348 4227
StyI C'CwwG_G
Cuts at: 513 1065 3014 513
Size: 552 1949 3074
Fragments arranged by size:
3074 1949 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2238 3602 3619 3772 3957 4402 325
Size: 1255 658 1364 17 153 185 445 1498
Fragments arranged by size:
1498 1364 1255 658 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2031 2040 2280
Size: 274 80 33 51 1443 54 9 240
Cuts at: 2280 3821 5533 96
Size: 1541 1712 138
Fragments arranged by size:
1712 1541 1443 274 240 138 80 54
51 33 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5535 5567 5535
Size: 32 5543
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5575
XbaI T'CTAG_A
Cuts at: 2632 2911 2632
Size: 279 5296
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5575
XmaI C'CCGG_G
Cuts at: 1870 2900 1870
Size: 1030 4545
XmnI GAAnn'nnTTC
Cuts at: 3704 3704
Size: 5575
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3383 276
Size: 53 83 186 936 1849 2468
Fragments arranged by size:
2468 1849 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PsrI PstI PvuI SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII
EcoNI EcoRV FalI FseI FspAI KasI NarI NruI
NsiI PacI PmlI PspOMI PspXI PvuII RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI