Restriction map for
pFN21AB8936
(Circular) (Six-base) MAPSORT of: pFN21AB8936.seq Check: 2152 from: 1 to: 5560
pFN21AB8936 5560 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2832 2832
Size: 5560
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3370 278
Size: 53 83 186 936 1834 2468
Fragments arranged by size:
2468 1834 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2903 1937
Size: 966 4594
Acc65I G'GTAC_C
Cuts at: 2881 2881
Size: 5560
AclI AA'CG_TT
Cuts at: 1563 3687 4060 1563
Size: 2124 373 3063
Fragments arranged by size:
3063 2124 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2152 3623 5007 5275 1632
Size: 520 1471 1384 268 1917
Fragments arranged by size:
1917 1471 1384 520 268
AflII C'TTAA_G
Cuts at: 820 1017 2837 820
Size: 197 1820 3543
Fragments arranged by size:
3543 1820 197
AflIII A'CryG_T
Cuts at: 1203 2137 2697 4459 5436 1203
Size: 934 560 1762 977 1327
Fragments arranged by size:
1762 1327 977 934 560
AgeI A'CCGG_T
Cuts at: 4391 4391
Size: 5560
AhdI GACnn_n'nnGTC
Cuts at: 4289 4289
Size: 5560
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5560
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5560
AlwNI CAG_nnn'CTG
Cuts at: 2764 3303 4875 2764
Size: 539 1572 3449
Fragments arranged by size:
3449 1572 539
ApaI G_GGCC'C
Cuts at: 2648 2648
Size: 5560
ApaLI G'TGCA_C
Cuts at: 2037 2090 2795 3617 4773 2037
Size: 53 705 822 1156 2824
Fragments arranged by size:
2824 1156 822 705 53
ApoI r'AATT_y
Cuts at: 1493 2869 3095 3247 5356 1493
Size: 1376 226 152 2109 1697
Fragments arranged by size:
2109 1697 1376 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4114 160
Size: 872 3082 1606
Fragments arranged by size:
3082 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5560
AvaI C'yCGr_G
Cuts at: 1870 1943 2051 2188 2294 2373 2469 2647 2655
Size: 73 108 137 106 79 96 178 8
Cuts at: 2655 2735 2885 1870
Size: 80 150 4545
Fragments arranged by size:
4545 178 150 137 108 106 96 80
79 73 8
BamHI G'GATC_C
Cuts at: 2890 3257 2890
Size: 367 5193
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2096 2881 4336 618
Size: 325 217 627 309 785 1455 1842
Fragments arranged by size:
1842 1455 785 627 325 309 217
BanII G_rGCy'C
Cuts at: 1391 2025 2067 2505 2648 2820 2879 1391
Size: 634 42 438 143 172 59 4072
Fragments arranged by size:
4072 634 438 172 143 59 42
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2191 2332 928
Size: 971 292 141 4156
Fragments arranged by size:
4156 971 292 141
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3751 3785 1903
Size: 34 1814 34 3678
Fragments arranged by size:
3678 1814 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3452 4662 3452
Size: 1210 4350
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5560
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2282 2484 2601
Size: 107 122 71 631 605 610 202 117
Cuts at: 2601 4171 136
Size: 1570 1525
Fragments arranged by size:
1570 1525 631 610 605 202 122 117
107 71
BglII A'GATC_T
Cuts at: 5555 5555
Size: 5560
BlpI GC'TnA_GC
Cuts at: 2977 2977
Size: 5560
Bme1580I G_kGCm'C
Cuts at: 1790 2041 2094 2373 2648 2799 3621 4777 1790
Size: 251 53 279 275 151 822 1156 2573
Fragments arranged by size:
2573 1156 822 279 275 251 151 53
BmgBI CAC'GTC
Cuts at: 1318 2698 1318
Size: 1380 4180
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3291 4249 442
Size: 416 53 83 399 24 1874 958 1753
Fragments arranged by size:
1874 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5560
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4220 1239
Size: 2981 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3685 4550 4848 3685
Size: 865 298 4397
Fragments arranged by size:
4397 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4223 882
Size: 617 2724 2219
Fragments arranged by size:
2724 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3351 493
Size: 711 2147 2702
Fragments arranged by size:
2702 2147 711
BsaBI GATnn'nnATC
Cuts at: 5554 5554
Size: 5560
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3367 3749 4384 275
Size: 53 83 186 936 1834 382 635 1451
Fragments arranged by size:
1834 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3993 4391 4665 4812 5223 3993
Size: 398 274 147 411 4330
Fragments arranged by size:
4330 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5335
Fragments arranged by size:
5335 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2231 2417 1186
Size: 1045 186 4329
Fragments arranged by size:
4329 1045 186
BseYI C'CCAG_C
Cuts at: 2227 2408 4763 5259 2227
Size: 181 2355 496 2528
Fragments arranged by size:
2528 2355 496 181
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4987
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2556 2610 3010 3771 4799 1540
Size: 275 186 555 54 400 761 1028 2301
Fragments arranged by size:
2301 1028 761 555 400 275 186 54
BsiHKAI G_wGCw'C
Cuts at: 2041 2094 2799 3621 3706 4777 2041
Size: 53 705 822 85 1071 2824
Fragments arranged by size:
2824 1071 822 705 85 53
BsmI GAATG_Cn'
Cuts at: 2463 3071 3164 2463
Size: 608 93 4859
Fragments arranged by size:
4859 608 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2255 2255
Size: 5560
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2025 2041 2067 2094 2373 2505 2648
Size: 399 235 16 26 27 279 132 143
Cuts at: 2648 2799 2820 2879 3621 3706 4777 1391
Size: 151 21 59 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 279 235 151 143
132 85 59 27 26 21 16
BspEI T'CCGG_A
Cuts at: 5223 5223
Size: 5560
BspHI T'CATG_A
Cuts at: 3449 5179 3449
Size: 1730 3830
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2832 2915 844
Size: 1988 83 3489
Fragments arranged by size:
3489 1988 83
BsrBI CCG'CTC
Cuts at: 1394 1823 3447 1394
Size: 429 1624 3507
Fragments arranged by size:
3507 1624 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4055 4229 65
Size: 1183 2807 174 1396
Fragments arranged by size:
2807 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2493 2799 4204 4391 1700
Size: 110 683 306 1405 187 2869
Fragments arranged by size:
2869 1405 683 306 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5560
BssHII G'CGCG_C
Cuts at: 1925 2558 1925
Size: 633 4927
BssSI C'ACGA_G
Cuts at: 2429 3620 4632 2429
Size: 1191 1012 3357
Fragments arranged by size:
3357 1191 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5560
BstBI TT'CG_AA
Cuts at: 4364 4364
Size: 5560
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5560
BstYI r'GATC_y
Cuts at: 1972 2890 3257 3642 3659 5111 5197 5209 5555
Size: 918 367 385 17 1452 86 12 346
Cuts at: 5555 1972
Size: 1977
Fragments arranged by size:
1977 1452 918 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2025 2155 513
Size: 552 939 21 130 3918
Fragments arranged by size:
3918 939 552 130 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4821
Fragments arranged by size:
4821 683 56
BtsI GCAGTG_nn'
Cuts at: 3076 3870 3890 3076
Size: 794 20 4746
Fragments arranged by size:
4746 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5560
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2122 2157 529
Size: 35 1558 35 3932
Fragments arranged by size:
3932 1558 35 35
DraI TTT'AAA
Cuts at: 2864 2864
Size: 5560
DraIII CAC_nnn'GTG
Cuts at: 2161 2161
Size: 5560
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2247 4567 809
Size: 507 931 2320 1802
Fragments arranged by size:
2320 1802 931 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2495 2607 2805
Size: 54 1425 50 275 18 665 112 198
Cuts at: 2805 3007 3896 5525 8
Size: 202 889 1629 43
Fragments arranged by size:
1629 1425 889 665 275 202 198 112
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2607 3007 1537
Size: 275 795 400 4090
Fragments arranged by size:
4090 795 400 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3490 1353
Size: 2137 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2408 4147 4521 4667 387
Size: 1064 138 819 1739 374 146 1280
Fragments arranged by size:
1739 1280 1064 819 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2152 3623 4220 5007 5275 1239
Size: 393 520 1471 597 787 268 1524
Fragments arranged by size:
1524 1471 787 597 520 393 268
EcoRI G'AATT_C
Cuts at: 2869 3247 2869
Size: 378 5182
FspI TGC'GCA
Cuts at: 2447 2673 4066 2447
Size: 226 1393 3941
Fragments arranged by size:
3941 1393 226
HaeII r_GCGC'y
Cuts at: 1678 2059 2655 4707 1678
Size: 381 596 2052 2531
Fragments arranged by size:
2531 2052 596 381
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4201 4233 4275 4307 1373
Size: 32 135 32 2629 32 42 32 2626
Fragments arranged by size:
2629 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2320 2904 3150 669
Size: 988 281 382 584 246 3079
Fragments arranged by size:
3079 988 584 382 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5560
HpaI GTT'AAC
Cuts at: 3150 3150
Size: 5560
KpnI G_GTAC'C
Cuts at: 2885 2885
Size: 5560
MfeI C'AATT_G
Cuts at: 3159 3159
Size: 5560
MluI A'CGCG_T
Cuts at: 2137 5436 2137
Size: 3299 2261
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2444 2576 4674 4858 1305
Size: 1139 132 2098 184 2007
Fragments arranged by size:
2098 2007 1139 184 132
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2807 10
Size: 54 1425 343 975 2763
Fragments arranged by size:
2763 1425 975 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2108 3518 3877 4036 518
Size: 648 942 1410 359 159 2042
Fragments arranged by size:
2042 1410 942 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2182 2402 2669 2976 3653 4801 5046 1606
Size: 576 220 267 307 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 576 307 267 245 220
NaeI GCC'GGC
Cuts at: 2495 2495
Size: 5560
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4069
Fragments arranged by size:
4069 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5560
NgoMIV G'CCGG_C
Cuts at: 2493 2493
Size: 5560
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5560
NotI GC'GGCC_GC
Cuts at: 3007 3007
Size: 5560
NspI r_CATG'y
Cuts at: 1132 1229 2617 2918 4463 1132
Size: 97 1388 301 1545 2229
Fragments arranged by size:
2229 1545 1388 301 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5560
PciI A'CATG_T
Cuts at: 4459 4459
Size: 5560
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2518 1108
Size: 1410 4150
PmeI GTTT'AAAC
Cuts at: 2864 2864
Size: 5560
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3629 3661 5142 5174 3629
Size: 32 1481 32 4015
Fragments arranged by size:
4015 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5560
PsiI TTA'TAA
Cuts at: 3130 3130
Size: 5560
PspOMI G'GGCC_C
Cuts at: 2644 2644
Size: 5560
PspXI vC'TCGA_Gb
Cuts at: 2051 2735 2051
Size: 684 4876
PstI C_TGCA'G
Cuts at: 830 2760 2912 830
Size: 1930 152 3478
Fragments arranged by size:
3478 1930 152
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5560
PvuII CAG'CTG
Cuts at: 2669 2669
Size: 5560
SalI G'TCGA_C
Cuts at: 1936 2902 1936
Size: 966 4594
SbfI CC_TGCA'GG
Cuts at: 2912 2912
Size: 5560
ScaI AGT'ACT
Cuts at: 3808 5520 3808
Size: 1712 3848
SfcI C'TryA_G
Cuts at: 826 1045 2506 2756 2908 4043 4724 4915 826
Size: 219 1461 250 152 1135 681 191 1471
Fragments arranged by size:
1471 1461 1135 681 250 219 191 152
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5560
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5560
SmaI CCC'GGG
Cuts at: 1872 2190 2375 2471 2657 2887 1872
Size: 318 185 96 186 230 4545
Fragments arranged by size:
4545 318 230 186 185 96
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2051 2735 2837 3664 4565 4827
Size: 197 926 108 684 102 827 901 262
Cuts at: 4827 820
Size: 1553
Fragments arranged by size:
1553 926 901 827 684 262 197 108
102
SnaBI TAC'GTA
Cuts at: 493 3351 493
Size: 2858 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5560
SphI G_CATG'C
Cuts at: 1132 2918 1132
Size: 1786 3774
SspI AAT'ATT
Cuts at: 5 52 3484 5
Size: 47 3432 2081
Fragments arranged by size:
3432 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5560
StyI C'CwwG_G
Cuts at: 513 1065 2004 2999 513
Size: 552 939 995 3074
Fragments arranged by size:
3074 995 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2243 2564 3587 3604 3757 3942 4387
Size: 1255 663 321 1023 17 153 185 445
Cuts at: 4387 325
Size: 1498
Fragments arranged by size:
1498 1255 1023 663 445 321 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3806 5518 96
Size: 274 80 33 51 1443 1829 1712 138
Fragments arranged by size:
1829 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5520 5552 5520
Size: 32 5528
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5560
XbaI T'CTAG_A
Cuts at: 2896 2896
Size: 5560
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2107 2738 2107
Size: 631 4929
XhoI C'TCGA_G
Cuts at: 1943 2051 2735 1943
Size: 108 684 4768
Fragments arranged by size:
4768 684 108
XmaI C'CCGG_G
Cuts at: 1870 2188 2373 2469 2655 2885 1870
Size: 318 185 96 186 230 4545
Fragments arranged by size:
4545 318 230 186 185 96
XmnI GAAnn'nnTTC
Cuts at: 3689 3689
Size: 5560
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3368 276
Size: 53 83 186 936 1834 2468
Fragments arranged by size:
2468 1834 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI
NsiI PacI PfoI PmlI PpuMI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI