Restriction map for
pFN21AB8934
(Circular) (Six-base) MAPSORT of: pFN21AB8934.seq Check: 9735 from: 1 to: 5557
pFN21AB8934 5557 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2225 3367 278
Size: 53 83 186 936 689 1142 2468
Fragments arranged by size:
2468 1142 936 689 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2900 1937
Size: 963 4594
Acc65I G'GTAC_C
Cuts at: 2878 2878
Size: 5557
AclI AA'CG_TT
Cuts at: 1563 3684 4057 1563
Size: 2121 373 3063
Fragments arranged by size:
3063 2121 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3620 5004 5272 1632
Size: 1988 1384 268 1917
Fragments arranged by size:
1988 1917 1384 268
AfeI AGC'GCT
Cuts at: 2848 2848
Size: 5557
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5360
AflIII A'CryG_T
Cuts at: 1203 2439 4456 5433 1203
Size: 1236 2017 977 1327
Fragments arranged by size:
2017 1327 1236 977
AgeI A'CCGG_T
Cuts at: 4388 4388
Size: 5557
AhdI GACnn_n'nnGTC
Cuts at: 4286 4286
Size: 5557
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5557
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5557
AlwNI CAG_nnn'CTG
Cuts at: 2458 3300 4872 2458
Size: 842 1572 3143
Fragments arranged by size:
3143 1572 842
ApaLI G'TGCA_C
Cuts at: 3614 4770 3614
Size: 1156 4401
ApoI r'AATT_y
Cuts at: 1493 2866 3092 3244 5353 1493
Size: 1373 226 152 2109 1697
Fragments arranged by size:
2109 1697 1373 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4111 160
Size: 872 3079 1606
Fragments arranged by size:
3079 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5557
AvaI C'yCGr_G
Cuts at: 1870 1943 2882 1870
Size: 73 939 4545
Fragments arranged by size:
4545 939 73
BamHI G'GATC_C
Cuts at: 2887 3254 2887
Size: 367 5190
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2119 2209 2878 4333 618
Size: 325 217 627 332 90 669 1455 1842
Fragments arranged by size:
1842 1455 669 627 332 325 217 90
BanII G_rGCy'C
Cuts at: 1391 2013 2291 2524 2876 1391
Size: 622 278 233 352 4072
Fragments arranged by size:
4072 622 352 278 233
BbeI G_GCGC'C
Cuts at: 2123 2123
Size: 5557
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4586
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3748 3782 1903
Size: 34 1811 34 3678
Fragments arranged by size:
3678 1811 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3449 4659 3449
Size: 1210 4347
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5557
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2321 4168 136
Size: 107 122 71 631 605 649 1847 1525
Fragments arranged by size:
1847 1525 649 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2748 5552 2748
Size: 2804 2753
BlpI GC'TnA_GC
Cuts at: 2974 2974
Size: 5557
Bme1580I G_kGCm'C
Cuts at: 1790 2214 2308 3618 4774 1790
Size: 424 94 1310 1156 2573
Fragments arranged by size:
2573 1310 1156 424 94
BmgBI CAC'GTC
Cuts at: 1318 2440 1318
Size: 1122 4435
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3288 4246 442
Size: 416 53 83 399 24 1871 958 1753
Fragments arranged by size:
1871 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5557
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2490 2593 2717 4217 1239
Size: 1251 103 124 1500 2579
Fragments arranged by size:
2579 1500 1251 124 103
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3682 4547 4845 3682
Size: 865 298 4394
Fragments arranged by size:
4394 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4220 882
Size: 617 2721 2219
Fragments arranged by size:
2721 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3348 493
Size: 711 2144 2702
Fragments arranged by size:
2702 2144 711
BsaBI GATnn'nnATC
Cuts at: 2786 5551 2786
Size: 2765 2792
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2120 2222 2631 3364
Size: 53 83 186 936 587 102 409 733
Cuts at: 3364 3746 4381 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 733 635 587 409 382 186
102 83 53
BsaWI w'CCGG_w
Cuts at: 3990 4388 4662 4809 5220 3990
Size: 398 274 147 411 4327
Fragments arranged by size:
4327 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2690 2720 1178
Size: 30 165 30 1287 30 4015
Fragments arranged by size:
4015 1287 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5557
BseYI C'CCAG_C
Cuts at: 2059 2113 2227 2366 4760 5256 2059
Size: 54 114 139 2394 496 2360
Fragments arranged by size:
2394 2360 496 139 114 54
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2471 1163
Size: 573 735 4249
Fragments arranged by size:
4249 735 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2352 2653 3007 3768 4796 1540
Size: 275 186 351 301 354 761 1028 2301
Fragments arranged by size:
2301 1028 761 354 351 301 275 186
BsiHKAI G_wGCw'C
Cuts at: 2291 2467 3618 3703 4774 2291
Size: 176 1151 85 1071 3074
Fragments arranged by size:
3074 1151 1071 176 85
BsmI GAATG_Cn'
Cuts at: 2664 3068 3161 2664
Size: 404 93 5060
Fragments arranged by size:
5060 404 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2214 2291 2308 2467 2524 2876
Size: 399 223 201 77 17 159 57 352
Cuts at: 2876 3618 3703 4774 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 352 223 201 159
85 77 57 17
BspEI T'CCGG_A
Cuts at: 5220 5220
Size: 5557
BspHI T'CATG_A
Cuts at: 3446 5176 3446
Size: 1730 3827
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2912 844
Size: 2068 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2066 2701 3444 1394
Size: 429 243 635 743 3507
Fragments arranged by size:
3507 743 635 429 243
BsrDI GCAATG_nn'
Cuts at: 65 1248 2195 4052 4226 65
Size: 1183 947 1857 174 1396
Fragments arranged by size:
1857 1396 1183 947 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4201 4388 1700
Size: 110 2391 187 2869
Fragments arranged by size:
2869 2391 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5557
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5557
BssSI C'ACGA_G
Cuts at: 3617 4629 3617
Size: 1012 4545
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2600 794
Size: 1806 3751
BstBI TT'CG_AA
Cuts at: 4361 4361
Size: 5557
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5557
BstXI CCAn_nnnn'nTGG
Cuts at: 2434 2434
Size: 5557
BstYI r'GATC_y
Cuts at: 1972 2158 2748 2887 3254 3639 3656 5108 5194
Size: 186 590 139 367 385 17 1452 86
Cuts at: 5194 5206 5552 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 590 385 367 346 186 139
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2334 2346 513
Size: 552 939 330 12 3724
Fragments arranged by size:
3724 939 552 330 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4818
Fragments arranged by size:
4818 683 56
BtsI GCAGTG_nn'
Cuts at: 3073 3867 3887 3073
Size: 794 20 4743
Fragments arranged by size:
4743 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5557
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2365 2400 529
Size: 35 1801 35 3686
Fragments arranged by size:
3686 1801 35 35
DraI TTT'AAA
Cuts at: 2861 2861
Size: 5557
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4564 809
Size: 507 3248 1802
Fragments arranged by size:
3248 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2322 2349 2424
Size: 54 1425 50 275 18 492 27 75
Cuts at: 2424 2650 3004 3893 5522 8
Size: 226 354 889 1629 43
Fragments arranged by size:
1629 1425 889 492 354 275 226 75
54 50 43 27 18
EagI C'GGCC_G
Cuts at: 1537 1812 2349 2650 3004 1537
Size: 275 537 301 354 4090
Fragments arranged by size:
4090 537 354 301 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2352 3487 1353
Size: 999 1135 3423
Fragments arranged by size:
3423 1135 999
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2092 2189 4144 4518 4664 387
Size: 1064 138 503 97 1955 374 146 1280
Fragments arranged by size:
1955 1280 1064 503 374 146 138 97
EcoICRI GAG'CTC
Cuts at: 2289 2289
Size: 5557
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2490 2593 2717 3620 4217 5004 5272
Size: 393 858 103 124 903 597 787 268
Cuts at: 5272 1239
Size: 1524
Fragments arranged by size:
1524 903 858 787 597 393 268 124
103
EcoO109I rG'GnC_Cy
Cuts at: 2579 2579
Size: 5557
EcoRI G'AATT_C
Cuts at: 2866 3244 2866
Size: 378 5179
EcoRV GAT'ATC
Cuts at: 2047 2402 2047
Size: 355 5202
FspI TGC'GCA
Cuts at: 4063 4063
Size: 5557
HaeII r_GCGC'y
Cuts at: 1678 2123 2779 2850 4704 1678
Size: 445 656 71 1854 2531
Fragments arranged by size:
2531 1854 656 445 71
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4198 4230 4272 4304 1373
Size: 32 135 32 2626 32 42 32 2626
Fragments arranged by size:
2626 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2143 2901 3147 669
Size: 988 281 205 758 246 3079
Fragments arranged by size:
3079 988 758 281 246 205
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5557
HpaI GTT'AAC
Cuts at: 3147 3147
Size: 5557
KasI G'GCGC_C
Cuts at: 2119 2119
Size: 5557
KpnI G_GTAC'C
Cuts at: 2882 2882
Size: 5557
MfeI C'AATT_G
Cuts at: 3156 3156
Size: 5557
MluI A'CGCG_T
Cuts at: 5433 5433
Size: 5557
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4671 4855 1305
Size: 3366 184 2007
Fragments arranged by size:
3366 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2426 10
Size: 54 1425 343 594 3141
Fragments arranged by size:
3141 1425 594 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3515 3874 4033 518
Size: 648 2349 359 159 2042
Fragments arranged by size:
2349 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2117 2336 2613 2973 3650 4798 5043 1606
Size: 511 219 277 360 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 511 360 277 245 219
NarI GG'CG_CC
Cuts at: 2120 2120
Size: 5557
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4066
Fragments arranged by size:
4066 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5557
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5557
NotI GC'GGCC_GC
Cuts at: 2650 3004 2650
Size: 354 5203
NspI r_CATG'y
Cuts at: 1132 1229 2915 4460 1132
Size: 97 1686 1545 2229
Fragments arranged by size:
2229 1686 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2204 2215 1348
Size: 856 11 4690
Fragments arranged by size:
4690 856 11
PciI A'CATG_T
Cuts at: 4456 4456
Size: 5557
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2204 2422 1108
Size: 1096 218 4243
Fragments arranged by size:
4243 1096 218
PfoI T'CCnGG_A
Cuts at: 2282 2395 2404 2467 2282
Size: 113 9 63 5372
Fragments arranged by size:
5372 113 63 9
PmeI GTTT'AAAC
Cuts at: 2861 2861
Size: 5557
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3626 3658 5139 5171 3626
Size: 32 1481 32 4012
Fragments arranged by size:
4012 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2579 2579
Size: 5557
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5557
PsiI TTA'TAA
Cuts at: 3127 3127
Size: 5557
PstI C_TGCA'G
Cuts at: 830 2909 830
Size: 2079 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5557
SacI G_AGCT'C
Cuts at: 2291 2291
Size: 5557
SacII CC_GC'GG
Cuts at: 2337 2337
Size: 5557
SalI G'TCGA_C
Cuts at: 1936 2899 1936
Size: 963 4594
SbfI CC_TGCA'GG
Cuts at: 2909 2909
Size: 5557
ScaI AGT'ACT
Cuts at: 3805 5517 3805
Size: 1712 3845
SexAI A'CCwGG_T
Cuts at: 2575 2575
Size: 5557
SfcI C'TryA_G
Cuts at: 826 1045 2905 4040 4721 4912 826
Size: 219 1860 1135 681 191 1471
Fragments arranged by size:
1860 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2121 2121
Size: 5557
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5557
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5557
SmaI CCC'GGG
Cuts at: 1872 2884 1872
Size: 1012 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3661 4562 4824 820
Size: 197 926 1718 901 262 1553
Fragments arranged by size:
1718 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3348 493
Size: 2855 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5557
SphI G_CATG'C
Cuts at: 1132 2915 1132
Size: 1783 3774
SspI AAT'ATT
Cuts at: 5 52 3481 5
Size: 47 3429 2081
Fragments arranged by size:
3429 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5557
StyI C'CwwG_G
Cuts at: 513 1065 2004 2028 2040 2154 2730 2832 2996
Size: 552 939 24 12 114 576 102 164
Cuts at: 2996 513
Size: 3074
Fragments arranged by size:
3074 939 576 552 164 114 102 24
12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2127 2211 2450 3584 3601 3754 3939
Size: 1255 547 84 239 1134 17 153 185
Cuts at: 3939 4384 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1134 547 445 239 185 153
84 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3803 5515 96
Size: 274 80 33 51 1443 1826 1712 138
Fragments arranged by size:
1826 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5517 5549 5517
Size: 32 5525
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5557
XbaI T'CTAG_A
Cuts at: 2893 2893
Size: 5557
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2201 2583 2201
Size: 382 5175
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5557
XmaI C'CCGG_G
Cuts at: 1870 2882 1870
Size: 1012 4545
XmnI GAAnn'nnTTC
Cuts at: 3686 3686
Size: 5557
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2223 3365 276
Size: 53 83 186 936 689 1142 2468
Fragments arranged by size:
2468 1142 936 689 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI SacI SacII SalI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PmlI PspOMI PspXI PsrI PvuII RsrII SanDI SapI
SfiI SrfI SwaI