Restriction map for
pFN21AB8930
(Circular) (Six-base) MAPSORT of: pFN21AB8930.seq Check: 3288 from: 1 to: 5548
pFN21AB8930 5548 bp
With 182 enzymes: SgfI *
December 8, 2014 13:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2079 2187 2079
Size: 108 5440
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3358 278
Size: 53 83 186 936 1822 2468
Fragments arranged by size:
2468 1822 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2891 1937
Size: 954 4594
Acc65I G'GTAC_C
Cuts at: 2869 2869
Size: 5548
AclI AA'CG_TT
Cuts at: 1563 3675 4048 1563
Size: 2112 373 3063
Fragments arranged by size:
3063 2112 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3611 4995 5263 1632
Size: 1979 1384 268 1917
Fragments arranged by size:
1979 1917 1384 268
AfeI AGC'GCT
Cuts at: 2432 2432
Size: 5548
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5351
AflIII A'CryG_T
Cuts at: 1203 4447 5424 1203
Size: 3244 977 1327
Fragments arranged by size:
3244 1327 977
AgeI A'CCGG_T
Cuts at: 4379 4379
Size: 5548
AhdI GACnn_n'nnGTC
Cuts at: 2578 4277 2578
Size: 1699 3849
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5548
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5548
AlwNI CAG_nnn'CTG
Cuts at: 2488 3291 4863 2488
Size: 803 1572 3173
Fragments arranged by size:
3173 1572 803
ApaI G_GGCC'C
Cuts at: 2600 2600
Size: 5548
ApaLI G'TGCA_C
Cuts at: 3605 4761 3605
Size: 1156 4392
ApoI r'AATT_y
Cuts at: 1493 2857 3083 3235 5344 1493
Size: 1364 226 152 2109 1697
Fragments arranged by size:
2109 1697 1364 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4102 160
Size: 872 3070 1606
Fragments arranged by size:
3070 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5548
AvaI C'yCGr_G
Cuts at: 1870 1943 2636 2737 2873 1870
Size: 73 693 101 136 4545
Fragments arranged by size:
4545 693 136 101 73
BamHI G'GATC_C
Cuts at: 2878 3245 2878
Size: 367 5181
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2032 2723 2747 2869 4324
Size: 325 217 627 245 691 24 122 1455
Cuts at: 4324 618
Size: 1842
Fragments arranged by size:
1842 1455 691 627 325 245 217 122
24
BanII G_rGCy'C
Cuts at: 1391 2277 2600 2867 1391
Size: 886 323 267 4072
Fragments arranged by size:
4072 886 323 267
BbeI G_GCGC'C
Cuts at: 2036 2727 2751 2036
Size: 691 24 4833
Fragments arranged by size:
4833 691 24
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2829 928
Size: 971 930 3647
Fragments arranged by size:
3647 971 930
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3739 3773 1903
Size: 34 1802 34 3678
Fragments arranged by size:
3678 1802 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3440 4650 3440
Size: 1210 4338
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5548
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2732 4159 136
Size: 107 122 71 631 605 1060 1427 1525
Fragments arranged by size:
1525 1427 1060 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2412 5543 2412
Size: 3131 2417
BlpI GC'TnA_GC
Cuts at: 2538 2965 2538
Size: 427 5121
Bme1580I G_kGCm'C
Cuts at: 1790 2600 3609 4765 1790
Size: 810 1009 1156 2573
Fragments arranged by size:
2573 1156 1009 810
BmgBI CAC'GTC
Cuts at: 1318 2839 1318
Size: 1521 4027
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2603 3279 4237
Size: 416 53 83 399 24 1186 676 958
Cuts at: 4237 442
Size: 1753
Fragments arranged by size:
1753 1186 958 676 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5548
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2662 2662
Size: 5548
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2288 2321 2576 4208 1239
Size: 1049 33 255 1632 2579
Fragments arranged by size:
2579 1632 1049 255 33
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2528 3673 4538 4836 2528
Size: 1145 865 298 3240
Fragments arranged by size:
3240 1145 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4211 882
Size: 617 2712 2219
Fragments arranged by size:
2712 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3339 493
Size: 711 2135 2702
Fragments arranged by size:
2702 2135 711
BsaBI GATnn'nnATC
Cuts at: 2615 5542 2615
Size: 2927 2621
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2033 2105 2724 2748
Size: 53 83 186 936 500 72 619 24
Cuts at: 2748 3355 3737 4372 275
Size: 607 382 635 1451
Fragments arranged by size:
1451 936 635 619 607 500 382 186
83 72 53 24
BsaWI w'CCGG_w
Cuts at: 2707 3981 4379 4653 4800 5211 2707
Size: 1274 398 274 147 411 3044
Fragments arranged by size:
3044 1274 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5323
Fragments arranged by size:
5323 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2057 2285 2573 1186
Size: 871 228 288 4161
Fragments arranged by size:
4161 871 288 228
BseYI C'CCAG_C
Cuts at: 2488 2600 4751 5247 2488
Size: 112 2151 496 2789
Fragments arranged by size:
2789 2151 496 112
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4975
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2467 2998 3759 4787 1540
Size: 275 186 466 531 761 1028 2301
Fragments arranged by size:
2301 1028 761 531 466 275 186
BsiHKAI G_wGCw'C
Cuts at: 2277 3609 3694 4765 2277
Size: 1332 85 1071 3060
Fragments arranged by size:
3060 1332 1071 85
BsmI GAATG_Cn'
Cuts at: 3059 3152 3059
Size: 93 5455
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2277 2600 2867 3609 3694 4765 1391
Size: 399 487 323 267 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 487 399 323 267 85
BspEI T'CCGG_A
Cuts at: 5211 5211
Size: 5548
BspHI T'CATG_A
Cuts at: 3437 5167 3437
Size: 1730 3818
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2079 2187 2903 844
Size: 1235 108 716 3489
Fragments arranged by size:
3489 1235 716 108
BsrBI CCG'CTC
Cuts at: 1394 1823 2047 2776 3435 1394
Size: 429 224 729 659 3507
Fragments arranged by size:
3507 729 659 429 224
BsrDI GCAATG_nn'
Cuts at: 65 1248 4043 4217 65
Size: 1183 2795 174 1396
Fragments arranged by size:
2795 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2660 2788 4192 4379 1700
Size: 110 850 128 1404 187 2869
Fragments arranged by size:
2869 1404 850 187 128 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5548
BssHII G'CGCG_C
Cuts at: 1925 2232 2262 2533 1925
Size: 307 30 271 4940
Fragments arranged by size:
4940 307 271 30
BssSI C'ACGA_G
Cuts at: 3608 4620 3608
Size: 1012 4536
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5548
BstBI TT'CG_AA
Cuts at: 4352 4352
Size: 5548
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5548
BstXI CCAn_nnnn'nTGG
Cuts at: 2593 2593
Size: 5548
BstYI r'GATC_y
Cuts at: 1972 2412 2878 3245 3630 3647 5099 5185 5197
Size: 440 466 367 385 17 1452 86 12
Cuts at: 5197 5543 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 466 440 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2190 513
Size: 552 1125 3871
Fragments arranged by size:
3871 1125 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4809
Fragments arranged by size:
4809 683 56
BtsI GCAGTG_nn'
Cuts at: 3064 3858 3878 3064
Size: 794 20 4734
Fragments arranged by size:
4734 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5548
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5513
DraI TTT'AAA
Cuts at: 2852 2852
Size: 5548
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4555 809
Size: 507 3239 1802
Fragments arranged by size:
3239 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2184 2628 2790
Size: 54 1425 50 275 18 354 444 162
Cuts at: 2790 2995 3884 5513 8
Size: 205 889 1629 43
Fragments arranged by size:
1629 1425 889 444 354 275 205 162
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2995 1537
Size: 275 1183 4090
Fragments arranged by size:
4090 1183 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2823 3478 1353
Size: 1470 655 3423
Fragments arranged by size:
3423 1470 655
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2067 2572 4135 4509 4655 387
Size: 1064 138 478 505 1563 374 146 1280
Fragments arranged by size:
1563 1280 1064 505 478 374 146 138
EcoICRI GAG'CTC
Cuts at: 2275 2275
Size: 5548
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2288 2321 2576 3611 4208 4995 5263
Size: 393 656 33 255 1035 597 787 268
Cuts at: 5263 1239
Size: 1524
Fragments arranged by size:
1524 1035 787 656 597 393 268 255
33
EcoO109I rG'GnC_Cy
Cuts at: 2551 2597 2734 2551
Size: 46 137 5365
Fragments arranged by size:
5365 137 46
EcoRI G'AATT_C
Cuts at: 2857 3235 2857
Size: 378 5170
FspI TGC'GCA
Cuts at: 4054 4054
Size: 5548
HaeII r_GCGC'y
Cuts at: 1678 2036 2434 2627 2727 2745 2751 4695 1678
Size: 358 398 193 100 18 6 1944 2531
Fragments arranged by size:
2531 1944 398 358 193 100 18 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2564 2596 4189 4221 4263
Size: 32 135 32 992 32 1593 32 42
Cuts at: 4263 4295 1373
Size: 32 2626
Fragments arranged by size:
2626 1593 992 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2892 3138 669
Size: 988 281 954 246 3079
Fragments arranged by size:
3079 988 954 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5548
HpaI GTT'AAC
Cuts at: 3138 3138
Size: 5548
KasI G'GCGC_C
Cuts at: 2032 2723 2747 2032
Size: 691 24 4833
Fragments arranged by size:
4833 691 24
KpnI G_GTAC'C
Cuts at: 2873 2873
Size: 5548
MfeI C'AATT_G
Cuts at: 3147 3147
Size: 5548
MluI A'CGCG_T
Cuts at: 5424 5424
Size: 5548
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2115 4662 4846 1305
Size: 810 2547 184 2007
Fragments arranged by size:
2547 2007 810 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3726
Fragments arranged by size:
3726 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3506 3865 4024 518
Size: 648 2340 359 159 2042
Fragments arranged by size:
2340 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2062 2192 2257 2359 2488 2604 2964 3641
Size: 456 130 65 102 129 116 360 677
Cuts at: 3641 4789 5034 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 456 360 245 130 129
116 102 65
NaeI GCC'GGC
Cuts at: 2662 2790 2662
Size: 128 5420
NarI GG'CG_CC
Cuts at: 2033 2724 2748 2033
Size: 691 24 4833
Fragments arranged by size:
4833 691 24
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4996
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5548
NgoMIV G'CCGG_C
Cuts at: 2660 2788 2660
Size: 128 5420
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5548
NotI GC'GGCC_GC
Cuts at: 2995 2995
Size: 5548
NruI TCG'CGA
Cuts at: 2469 2469
Size: 5548
NspI r_CATG'y
Cuts at: 1132 1229 2906 4451 1132
Size: 97 1677 1545 2229
Fragments arranged by size:
2229 1677 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5548
PciI A'CATG_T
Cuts at: 4447 4447
Size: 5548
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5548
PmeI GTTT'AAAC
Cuts at: 2852 2852
Size: 5548
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3617 3649 5130 5162 3617
Size: 32 1481 32 4003
Fragments arranged by size:
4003 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2551 2551
Size: 5548
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5548
PsiI TTA'TAA
Cuts at: 3118 3118
Size: 5548
PspOMI G'GGCC_C
Cuts at: 2596 2596
Size: 5548
PspXI vC'TCGA_Gb
Cuts at: 2636 2636
Size: 5548
PstI C_TGCA'G
Cuts at: 830 2319 2757 2900 830
Size: 1489 438 143 3478
Fragments arranged by size:
3478 1489 438 143
PvuI CG_AT'CG
Cuts at: 2001 2467 2001
Size: 466 5082
PvuII CAG'CTG
Cuts at: 2488 2488
Size: 5548
SacI G_AGCT'C
Cuts at: 2277 2277
Size: 5548
SacII CC_GC'GG
Cuts at: 2193 2193
Size: 5548
SalI G'TCGA_C
Cuts at: 1936 2890 1936
Size: 954 4594
SbfI CC_TGCA'GG
Cuts at: 2900 2900
Size: 5548
ScaI AGT'ACT
Cuts at: 3796 5508 3796
Size: 1712 3836
SexAI A'CCwGG_T
Cuts at: 2516 2516
Size: 5548
SfcI C'TryA_G
Cuts at: 826 1045 2315 2753 2896 4031 4712 4903 826
Size: 219 1270 438 143 1135 681 191 1471
Fragments arranged by size:
1471 1270 1135 681 438 219 191 143
SfoI GGC'GCC
Cuts at: 2034 2725 2749 2034
Size: 691 24 4833
Fragments arranged by size:
4833 691 24
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5548
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5548
SmaI CCC'GGG
Cuts at: 1872 2739 2875 1872
Size: 867 136 4545
Fragments arranged by size:
4545 867 136
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2543 2636 3652 4553 4815 820
Size: 197 926 600 93 1016 901 262 1553
Fragments arranged by size:
1553 1016 926 901 600 262 197 93
SnaBI TAC'GTA
Cuts at: 493 3339 493
Size: 2846 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5548
SphI G_CATG'C
Cuts at: 1132 2906 1132
Size: 1774 3774
SspI AAT'ATT
Cuts at: 5 52 3472 5
Size: 47 3420 2081
Fragments arranged by size:
3420 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5548
StyI C'CwwG_G
Cuts at: 513 1065 2013 2987 513
Size: 552 948 974 3074
Fragments arranged by size:
3074 974 948 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3575 3592 3745 3930 4375 325
Size: 1255 1995 17 153 185 445 1498
Fragments arranged by size:
1995 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3794 5506 96
Size: 274 80 33 51 1443 1817 1712 138
Fragments arranged by size:
1817 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5508 5540 5508
Size: 32 5516
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5548
XbaI T'CTAG_A
Cuts at: 2884 2884
Size: 5548
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2608 2608
Size: 5548
XhoI C'TCGA_G
Cuts at: 1943 2636 1943
Size: 693 4855
XmaI C'CCGG_G
Cuts at: 1870 2737 2873 1870
Size: 867 136 4545
Fragments arranged by size:
4545 867 136
XmnI GAAnn'nnTTC
Cuts at: 3677 3677
Size: 5548
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3356 276
Size: 53 83 186 936 1822 2468
Fragments arranged by size:
2468 1822 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacI SacII SalI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI
FspAI NsiI PacI PfoI PmlI PsrI RsrII SanDI
SapI SfiI SrfI SwaI