Restriction map for
pFN21AB8929
(Circular) (Six-base) MAPSORT of: pFN21AB8929.seq Check: 6704 from: 1 to: 5548
pFN21AB8929 5548 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2475 2475
Size: 5548
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3358 278
Size: 53 83 186 936 1822 2468
Fragments arranged by size:
2468 1822 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2891 1937
Size: 954 4594
Acc65I G'GTAC_C
Cuts at: 2869 2869
Size: 5548
AclI AA'CG_TT
Cuts at: 1563 3675 4048 1563
Size: 2112 373 3063
Fragments arranged by size:
3063 2112 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2576 3611 4995 5263 1632
Size: 944 1035 1384 268 1917
Fragments arranged by size:
1917 1384 1035 944 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5351
AflIII A'CryG_T
Cuts at: 1203 2100 4447 5424 1203
Size: 897 2347 977 1327
Fragments arranged by size:
2347 1327 977 897
AgeI A'CCGG_T
Cuts at: 4379 4379
Size: 5548
AhdI GACnn_n'nnGTC
Cuts at: 4277 4277
Size: 5548
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5548
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2557 1184
Size: 1373 4175
AlwNI CAG_nnn'CTG
Cuts at: 2195 2500 3291 4863 2195
Size: 305 791 1572 2880
Fragments arranged by size:
2880 1572 791 305
ApaI G_GGCC'C
Cuts at: 2085 2219 2085
Size: 134 5414
ApaLI G'TGCA_C
Cuts at: 3605 4761 3605
Size: 1156 4392
ApoI r'AATT_y
Cuts at: 1493 2857 3083 3235 5344 1493
Size: 1364 226 152 2109 1697
Fragments arranged by size:
2109 1697 1364 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4102 160
Size: 872 3070 1606
Fragments arranged by size:
3070 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5548
AvaI C'yCGr_G
Cuts at: 1870 1943 2037 2057 2084 2376 2873 1870
Size: 73 94 20 27 292 497 4545
Fragments arranged by size:
4545 497 292 94 73 27 20
BamHI G'GATC_C
Cuts at: 2878 3245 2878
Size: 367 5181
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2560 2869 4324 618
Size: 325 217 627 773 309 1455 1842
Fragments arranged by size:
1842 1455 773 627 325 309 217
BanII G_rGCy'C
Cuts at: 1391 2037 2085 2219 2376 2867 1391
Size: 646 48 134 157 491 4072
Fragments arranged by size:
4072 646 491 157 134 48
BbeI G_GCGC'C
Cuts at: 2564 2564
Size: 5548
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4577
BbvCI CC'TCA_GC
Cuts at: 2630 2630
Size: 5548
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2159 2193 2632 2666 3739 3773 1903
Size: 34 222 34 439 34 1073 34 3678
Fragments arranged by size:
3678 1073 439 222 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3440 4650 3440
Size: 1210 4338
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5548
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2214 2380 2522
Size: 107 122 71 631 605 542 166 142
Cuts at: 2522 4159 136
Size: 1637 1525
Fragments arranged by size:
1637 1525 631 605 542 166 142 122
107 71
BglII A'GATC_T
Cuts at: 5543 5543
Size: 5548
BlpI GC'TnA_GC
Cuts at: 2965 2965
Size: 5548
Bme1580I G_kGCm'C
Cuts at: 1790 2085 2219 3609 4765 1790
Size: 295 134 1390 1156 2573
Fragments arranged by size:
2573 1390 1156 295 134
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5548
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3279 4237 442
Size: 416 53 83 399 24 1862 958 1753
Fragments arranged by size:
1862 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5548
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2714 2735 4208 1239
Size: 1475 21 1473 2579
Fragments arranged by size:
2579 1475 1473 21
Bpu10I CC'TnA_GC
Cuts at: 2630 2630
Size: 5548
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2195 2495 2639 3673 4538 4836 2195
Size: 300 144 1034 865 298 2907
Fragments arranged by size:
2907 1034 865 300 298 144
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4211 882
Size: 617 2712 2219
Fragments arranged by size:
2712 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3339 493
Size: 711 2135 2702
Fragments arranged by size:
2702 2135 711
BsaBI GATnn'nnATC
Cuts at: 5542 5542
Size: 5548
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2561 3355 3737 4372
Size: 53 83 186 936 1028 794 382 635
Cuts at: 4372 275
Size: 1451
Fragments arranged by size:
1451 1028 936 794 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3981 4379 4653 4800 5211 3981
Size: 398 274 147 411 4318
Fragments arranged by size:
4318 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5323
Fragments arranged by size:
5323 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2384 1186
Size: 1198 4350
BseYI C'CCAG_C
Cuts at: 2078 2308 4751 5247 2078
Size: 230 2443 496 2379
Fragments arranged by size:
2443 2379 496 230
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4975
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2998 3759 4787 1540
Size: 275 186 997 761 1028 2301
Fragments arranged by size:
2301 1028 997 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3609 3694 4765 3609
Size: 85 1071 4392
Fragments arranged by size:
4392 1071 85
BsmI GAATG_Cn'
Cuts at: 2688 3059 3152 2688
Size: 371 93 5084
Fragments arranged by size:
5084 371 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2690 2690
Size: 5548
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2037 2085 2219 2376 2867 3609 3694
Size: 399 247 48 134 157 491 742 85
Cuts at: 3694 4765 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 491 399 247 157 134
85 48
BspEI T'CCGG_A
Cuts at: 5211 5211
Size: 5548
BspHI T'CATG_A
Cuts at: 3437 5167 3437
Size: 1730 3818
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2475 2903 844
Size: 1631 428 3489
Fragments arranged by size:
3489 1631 428
BsrBI CCG'CTC
Cuts at: 1394 1823 3435 1394
Size: 429 1612 3507
Fragments arranged by size:
3507 1612 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4043 4217 65
Size: 1183 2795 174 1396
Fragments arranged by size:
2795 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2625 4192 4379 1700
Size: 110 815 1567 187 2869
Fragments arranged by size:
2869 1567 815 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5548
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5548
BssSI C'ACGA_G
Cuts at: 3608 4620 3608
Size: 1012 4536
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2710 794
Size: 1916 3632
BstBI TT'CG_AA
Cuts at: 4352 4352
Size: 5548
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5548
BstXI CCAn_nnnn'nTGG
Cuts at: 2356 2356
Size: 5548
BstYI r'GATC_y
Cuts at: 1972 2878 3245 3630 3647 5099 5185 5197 5543
Size: 906 367 385 17 1452 86 12 346
Cuts at: 5543 1972
Size: 1977
Fragments arranged by size:
1977 1452 906 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2196 2808 2196
Size: 612 4936
BtgI C'CryG_G
Cuts at: 513 1065 2011 513
Size: 552 946 4050
Fragments arranged by size:
4050 946 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2635 487
Size: 683 56 1409 3400
Fragments arranged by size:
3400 1409 683 56
BtsI GCAGTG_nn'
Cuts at: 2526 2607 3064 3858 3878 2526
Size: 81 457 794 20 4196
Fragments arranged by size:
4196 794 457 81 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5548
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5513
DraI TTT'AAA
Cuts at: 2852 2852
Size: 5548
DraIII CAC_nnn'GTG
Cuts at: 2447 2447
Size: 5548
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4555 809
Size: 507 3239 1802
Fragments arranged by size:
3239 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2044 2491 2523
Size: 54 1425 50 275 18 214 447 32
Cuts at: 2523 2814 2995 3884 5513 8
Size: 291 181 889 1629 43
Fragments arranged by size:
1629 1425 889 447 291 275 214 181
54 50 43 32 18
EagI C'GGCC_G
Cuts at: 1537 1812 2995 1537
Size: 275 1183 4090
Fragments arranged by size:
4090 1183 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2148 2798 3478 1353
Size: 795 650 680 3423
Fragments arranged by size:
3423 795 680 650
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2122 2786 4135 4509 4655 387
Size: 1064 138 533 664 1349 374 146 1280
Fragments arranged by size:
1349 1280 1064 664 533 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2576 2714 2735 3611 4208 4995 5263
Size: 393 944 138 21 876 597 787 268
Cuts at: 5263 1239
Size: 1524
Fragments arranged by size:
1524 944 876 787 597 393 268 138
21
EcoNI CCTnn'n_nnAGG
Cuts at: 2555 2825 2555
Size: 270 5278
EcoO109I rG'GnC_Cy
Cuts at: 2016 2215 2216 2312 2515 2016
Size: 199 1 96 203 5049
Fragments arranged by size:
5049 203 199 96 1
EcoRI G'AATT_C
Cuts at: 2857 3235 2857
Size: 378 5170
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2177 2633 2177
Size: 456 5092
FspI TGC'GCA
Cuts at: 2660 4054 2660
Size: 1394 4154
HaeII r_GCGC'y
Cuts at: 1678 2195 2309 2564 2740 4695 1678
Size: 517 114 255 176 1955 2531
Fragments arranged by size:
2531 1955 517 255 176 114
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4189 4221 4263 4295 1373
Size: 32 135 32 2617 32 42 32 2626
Fragments arranged by size:
2626 2617 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2892 3138 669
Size: 988 281 954 246 3079
Fragments arranged by size:
3079 988 954 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5548
HpaI GTT'AAC
Cuts at: 3138 3138
Size: 5548
KasI G'GCGC_C
Cuts at: 2560 2560
Size: 5548
KpnI G_GTAC'C
Cuts at: 2873 2873
Size: 5548
MfeI C'AATT_G
Cuts at: 3147 3147
Size: 5548
MluI A'CGCG_T
Cuts at: 5424 5424
Size: 5548
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4662 4846 1305
Size: 3357 184 2007
Fragments arranged by size:
3357 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2046 2525 10
Size: 54 1425 343 214 479 3033
Fragments arranged by size:
3033 1425 479 343 214 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2654 3506 3865 4024 518
Size: 648 1488 852 359 159 2042
Fragments arranged by size:
2042 1488 852 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2964 3641 4789 5034 1606
Size: 1358 677 1148 245 2120
Fragments arranged by size:
2120 1358 1148 677 245
NarI GG'CG_CC
Cuts at: 2561 2561
Size: 5548
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4996
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5548
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5548
NotI GC'GGCC_GC
Cuts at: 2995 2995
Size: 5548
NspI r_CATG'y
Cuts at: 1132 1229 2104 2906 4451 1132
Size: 97 875 802 1545 2229
Fragments arranged by size:
2229 1545 875 802 97
PasI CC'CwG_GG
Cuts at: 1348 2333 2349 1348
Size: 985 16 4547
Fragments arranged by size:
4547 985 16
PciI A'CATG_T
Cuts at: 2100 4447 2100
Size: 2347 3201
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5548
PmeI GTTT'AAAC
Cuts at: 2852 2852
Size: 5548
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3617 3649 5130 5162 3617
Size: 32 1481 32 4003
Fragments arranged by size:
4003 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2016 2016
Size: 5548
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5548
PsiI TTA'TAA
Cuts at: 3118 3118
Size: 5548
PspOMI G'GGCC_C
Cuts at: 2081 2215 2081
Size: 134 5414
PspXI vC'TCGA_Gb
Cuts at: 2057 2057
Size: 5548
PstI C_TGCA'G
Cuts at: 830 2430 2546 2900 830
Size: 1600 116 354 3478
Fragments arranged by size:
3478 1600 354 116
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5548
SalI G'TCGA_C
Cuts at: 1936 2890 1936
Size: 954 4594
SapI GCTCTTCn'nnn_
Cuts at: 2148 2148
Size: 5548
SbfI CC_TGCA'GG
Cuts at: 2900 2900
Size: 5548
ScaI AGT'ACT
Cuts at: 3796 5508 3796
Size: 1712 3836
SfcI C'TryA_G
Cuts at: 826 1045 2323 2426 2542 2896 4031 4712 4903
Size: 219 1278 103 116 354 1135 681 191
Cuts at: 4903 826
Size: 1471
Fragments arranged by size:
1471 1278 1135 681 354 219 191 116
103
SfiI GGCCn_nnn'nGGCC
Cuts at: 2214 2522 2214
Size: 308 5240
SfoI GGC'GCC
Cuts at: 2562 2562
Size: 5548
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5548
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5548
SmaI CCC'GGG
Cuts at: 1872 2039 2086 2378 2875 1872
Size: 167 47 292 497 4545
Fragments arranged by size:
4545 497 292 167 47
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2057 2210 2510 2618 3652 4553
Size: 197 926 114 153 300 108 1034 901
Cuts at: 4553 4815 820
Size: 262 1553
Fragments arranged by size:
1553 1034 926 901 300 262 197 153
114 108
SnaBI TAC'GTA
Cuts at: 493 3339 493
Size: 2846 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5548
SphI G_CATG'C
Cuts at: 1132 2906 1132
Size: 1774 3774
SspI AAT'ATT
Cuts at: 5 52 3472 5
Size: 47 3420 2081
Fragments arranged by size:
3420 2081 47
StuI AGG'CCT
Cuts at: 1517 2117 2208 1517
Size: 600 91 4857
Fragments arranged by size:
4857 600 91
StyI C'CwwG_G
Cuts at: 513 1065 2987 513
Size: 552 1922 3074
Fragments arranged by size:
3074 1922 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2780 3575 3592 3745 3930 4375 325
Size: 1255 1200 795 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1200 795 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2718 3794 5506
Size: 274 80 33 51 1443 741 1076 1712
Cuts at: 5506 96
Size: 138
Fragments arranged by size:
1712 1443 1076 741 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5508 5540 5508
Size: 32 5516
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5548
XbaI T'CTAG_A
Cuts at: 2884 2884
Size: 5548
XhoI C'TCGA_G
Cuts at: 1943 2057 1943
Size: 114 5434
XmaI C'CCGG_G
Cuts at: 1870 2037 2084 2376 2873 1870
Size: 167 47 292 497 4545
Fragments arranged by size:
4545 497 292 167 47
XmnI GAAnn'nnTTC
Cuts at: 3677 3677
Size: 5548
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3356 276
Size: 53 83 186 936 1822 2468
Fragments arranged by size:
2468 1822 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BstZ17I EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI
NsiI PacI PfoI PmlI PsrI PvuII RsrII SacI
SacII SanDI SexAI SrfI SwaI XcmI