Restriction map for
pFN21AB8917
(Circular) (Six-base) MAPSORT of: pFN21AB8917.seq Check: 9577 from: 1 to: 5485
pFN21AB8917 5485 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3295 278
Size: 53 83 186 936 1759 2468
Fragments arranged by size:
2468 1759 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2828 1937
Size: 891 4594
Acc65I G'GTAC_C
Cuts at: 2806 2806
Size: 5485
AclI AA'CG_TT
Cuts at: 1563 3612 3985 1563
Size: 2049 373 3063
Fragments arranged by size:
3063 2049 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3548 4932 5200 1632
Size: 1916 1384 268 1917
Fragments arranged by size:
1917 1916 1384 268
AfeI AGC'GCT
Cuts at: 2591 2591
Size: 5485
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5288
AflIII A'CryG_T
Cuts at: 1203 2427 4384 5361 1203
Size: 1224 1957 977 1327
Fragments arranged by size:
1957 1327 1224 977
AgeI A'CCGG_T
Cuts at: 4316 4316
Size: 5485
AhdI GACnn_n'nnGTC
Cuts at: 4214 4214
Size: 5485
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5485
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5485
AlwNI CAG_nnn'CTG
Cuts at: 2473 2617 3228 4800 2473
Size: 144 611 1572 3158
Fragments arranged by size:
3158 1572 611 144
ApaI G_GGCC'C
Cuts at: 2022 2022
Size: 5485
ApaLI G'TGCA_C
Cuts at: 3542 4698 3542
Size: 1156 4329
ApoI r'AATT_y
Cuts at: 1493 2794 3020 3172 5281 1493
Size: 1301 226 152 2109 1697
Fragments arranged by size:
2109 1697 1301 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4039 160
Size: 872 3007 1606
Fragments arranged by size:
3007 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5485
AvaI C'yCGr_G
Cuts at: 1870 1943 2294 2375 2441 2483 2682 2810 1870
Size: 73 351 81 66 42 199 128 4545
Fragments arranged by size:
4545 351 199 128 81 73 66 42
BamHI G'GATC_C
Cuts at: 2815 3182 2815
Size: 367 5118
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2134 2203 2264 2309 2379
Size: 325 217 627 347 69 61 45 70
Cuts at: 2379 2806 4261 618
Size: 427 1455 1842
Fragments arranged by size:
1842 1455 627 427 347 325 217 70
69 61 45
BanII G_rGCy'C
Cuts at: 1391 2022 2104 2804 1391
Size: 631 82 700 4072
Fragments arranged by size:
4072 700 631 82
BbeI G_GCGC'C
Cuts at: 2207 2268 2313 2383 2207
Size: 61 45 70 5309
Fragments arranged by size:
5309 70 61 45
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2073 928
Size: 971 174 4340
Fragments arranged by size:
4340 971 174
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3676 3710 1903
Size: 34 1739 34 3678
Fragments arranged by size:
3678 1739 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3377 4587 3377
Size: 1210 4275
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5485
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2099 4096
Size: 107 122 71 631 605 337 90 1997
Cuts at: 4096 136
Size: 1525
Fragments arranged by size:
1997 1525 631 605 337 122 107 90
71
BglII A'GATC_T
Cuts at: 5480 5480
Size: 5485
BlpI GC'TnA_GC
Cuts at: 2902 2902
Size: 5485
Bme1580I G_kGCm'C
Cuts at: 1790 2022 2214 3546 4702 1790
Size: 232 192 1332 1156 2573
Fragments arranged by size:
2573 1332 1156 232 192
BmgBI CAC'GTC
Cuts at: 1318 2196 1318
Size: 878 4607
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3216 4174 442
Size: 416 53 83 399 24 1799 958 1753
Fragments arranged by size:
1799 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5485
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2645 4145 1239
Size: 1406 1500 2579
Fragments arranged by size:
2579 1500 1406
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2342 3610 4475 4773 2342
Size: 1268 865 298 3054
Fragments arranged by size:
3054 1268 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4148 882
Size: 617 2649 2219
Fragments arranged by size:
2649 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2038 3276 493
Size: 711 834 1238 2702
Fragments arranged by size:
2702 1238 834 711
BsaBI GATnn'nnATC
Cuts at: 5479 5479
Size: 5485
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2204 2265 2310 2367
Size: 53 83 186 936 671 61 45 57
Cuts at: 2367 2380 3292 3674 4309 275
Size: 13 912 382 635 1451
Fragments arranged by size:
1451 936 912 671 635 382 186 83
61 57 53 45 13
BsaWI w'CCGG_w
Cuts at: 3918 4316 4590 4737 5148 3918
Size: 398 274 147 411 4255
Fragments arranged by size:
4255 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2558 2588 2618 2648 1178
Size: 30 165 30 1155 30 30 30 4015
Fragments arranged by size:
4015 1155 165 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2534 1186
Size: 1348 4137
BseYI C'CCAG_C
Cuts at: 2123 2250 2324 2540 4688 5184 2123
Size: 127 74 216 2148 496 2424
Fragments arranged by size:
2424 2148 496 216 127 74
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4912
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2337 2458 2935 3696 4724 1540
Size: 275 186 336 121 477 761 1028 2301
Fragments arranged by size:
2301 1028 761 477 336 275 186 121
BsiHKAI G_wGCw'C
Cuts at: 3546 3631 4702 3546
Size: 85 1071 4329
Fragments arranged by size:
4329 1071 85
BsiWI C'GTAC_G
Cuts at: 2241 2241
Size: 5485
BsmI GAATG_Cn'
Cuts at: 2996 3089 2996
Size: 93 5392
BsmBI CGTCTCn'nnnn_
Cuts at: 2320 2320
Size: 5485
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2022 2104 2214 2804 3546 3631 4702
Size: 399 232 82 110 590 742 85 1071
Cuts at: 4702 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 590 399 232 110 85
82
BspEI T'CCGG_A
Cuts at: 5148 5148
Size: 5485
BspHI T'CATG_A
Cuts at: 3374 5104 3374
Size: 1730 3755
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2698 2840 844
Size: 1854 142 3489
Fragments arranged by size:
3489 1854 142
BsrBI CCG'CTC
Cuts at: 1394 1823 2574 2704 3372 1394
Size: 429 751 130 668 3507
Fragments arranged by size:
3507 751 668 429 130
BsrDI GCAATG_nn'
Cuts at: 65 1248 3980 4154 65
Size: 1183 2732 174 1396
Fragments arranged by size:
2732 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2179 2501 4129 4316 1700
Size: 110 369 322 1628 187 2869
Fragments arranged by size:
2869 1628 369 322 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5485
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5485
BssSI C'ACGA_G
Cuts at: 2578 3545 4557 2578
Size: 967 1012 3506
Fragments arranged by size:
3506 1012 967
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2473 794
Size: 1679 3806
BstBI TT'CG_AA
Cuts at: 4289 4289
Size: 5485
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5485
BstXI CCAn_nnnn'nTGG
Cuts at: 2131 2131
Size: 5485
BstYI r'GATC_y
Cuts at: 1972 2815 3182 3567 3584 5036 5122 5134 5480
Size: 843 367 385 17 1452 86 12 346
Cuts at: 5480 1972
Size: 1977
Fragments arranged by size:
1977 1452 843 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2226 2550 513
Size: 552 1161 324 3448
Fragments arranged by size:
3448 1161 552 324
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4746
Fragments arranged by size:
4746 683 56
BtsI GCAGTG_nn'
Cuts at: 2190 2471 3001 3795 3815 2190
Size: 281 530 794 20 3860
Fragments arranged by size:
3860 794 530 281 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5485
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5450
DraI TTT'AAA
Cuts at: 2789 2789
Size: 5485
DraIII CAC_nnn'GTG
Cuts at: 2199 2199
Size: 5485
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4492 809
Size: 507 3176 1802
Fragments arranged by size:
3176 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2091 2334 2553
Size: 54 1425 50 275 18 261 243 219
Cuts at: 2553 2932 3821 5450 8
Size: 379 889 1629 43
Fragments arranged by size:
1629 1425 889 379 275 261 243 219
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2334 2932 1537
Size: 275 522 598 4090
Fragments arranged by size:
4090 598 522 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2645 3415 1353
Size: 1292 770 3423
Fragments arranged by size:
3423 1292 770
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2504 4072 4446 4592 387
Size: 1064 138 915 1568 374 146 1280
Fragments arranged by size:
1568 1280 1064 915 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2645 3548 4145 4932 5200 1239
Size: 393 1013 903 597 787 268 1524
Fragments arranged by size:
1524 1013 903 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2018 2018
Size: 5485
EcoRI G'AATT_C
Cuts at: 2794 3172 2794
Size: 378 5107
FspI TGC'GCA
Cuts at: 3991 3991
Size: 5485
HaeII r_GCGC'y
Cuts at: 1678 2207 2268 2313 2383 2593 4632 1678
Size: 529 61 45 70 210 2039 2531
Fragments arranged by size:
2531 2039 529 210 70 61 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2141 2173 2288 2320 4126
Size: 32 135 32 569 32 115 32 1806
Cuts at: 4126 4158 4200 4232 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1806 569 135 115 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2829 3075 669
Size: 988 281 891 246 3079
Fragments arranged by size:
3079 988 891 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5485
HpaI GTT'AAC
Cuts at: 3075 3075
Size: 5485
KasI G'GCGC_C
Cuts at: 2203 2264 2309 2379 2203
Size: 61 45 70 5309
Fragments arranged by size:
5309 70 61 45
KpnI G_GTAC'C
Cuts at: 2810 2810
Size: 5485
MfeI C'AATT_G
Cuts at: 3084 3084
Size: 5485
MluI A'CGCG_T
Cuts at: 5361 5361
Size: 5485
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4599 4783 1305
Size: 3294 184 2007
Fragments arranged by size:
3294 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2555 10
Size: 54 1425 343 723 2940
Fragments arranged by size:
2940 1425 723 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2061 3443 3802 3961 518
Size: 648 895 1382 359 159 2042
Fragments arranged by size:
2042 1382 895 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2127 2302 2901 3578 4726 4971 1606
Size: 521 175 599 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 599 521 245 175
NaeI GCC'GGC
Cuts at: 2181 2503 2181
Size: 322 5163
NarI GG'CG_CC
Cuts at: 2204 2265 2310 2380 2204
Size: 61 45 70 5309
Fragments arranged by size:
5309 70 61 45
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4933
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5485
NgoMIV G'CCGG_C
Cuts at: 2179 2501 2179
Size: 322 5163
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5485
NotI GC'GGCC_GC
Cuts at: 2932 2932
Size: 5485
NspI r_CATG'y
Cuts at: 1132 1229 2284 2431 2843 4388 1132
Size: 97 1055 147 412 1545 2229
Fragments arranged by size:
2229 1545 1055 412 147 97
PasI CC'CwG_GG
Cuts at: 1348 2399 1348
Size: 1051 4434
PciI A'CATG_T
Cuts at: 2427 4384 2427
Size: 1957 3528
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2617 1108
Size: 1509 3976
PmeI GTTT'AAAC
Cuts at: 2789 2789
Size: 5485
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3554 3586 5067 5099 3554
Size: 32 1481 32 3940
Fragments arranged by size:
3940 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5485
PsiI TTA'TAA
Cuts at: 3055 3055
Size: 5485
PspOMI G'GGCC_C
Cuts at: 2018 2018
Size: 5485
PspXI vC'TCGA_Gb
Cuts at: 2294 2294
Size: 5485
PstI C_TGCA'G
Cuts at: 830 2837 830
Size: 2007 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5485
SalI G'TCGA_C
Cuts at: 1936 2827 1936
Size: 891 4594
SbfI CC_TGCA'GG
Cuts at: 2837 2837
Size: 5485
ScaI AGT'ACT
Cuts at: 3733 5445 3733
Size: 1712 3773
SfcI C'TryA_G
Cuts at: 826 1045 2143 2161 2833 3968 4649 4840 826
Size: 219 1098 18 672 1135 681 191 1471
Fragments arranged by size:
1471 1135 1098 681 672 219 191 18
SfoI GGC'GCC
Cuts at: 2205 2266 2311 2381 2205
Size: 61 45 70 5309
Fragments arranged by size:
5309 70 61 45
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5485
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5485
SmaI CCC'GGG
Cuts at: 1872 2377 2443 2684 2812 1872
Size: 505 66 241 128 4545
Fragments arranged by size:
4545 505 241 128 66
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2294 2357 3589 4490 4752 820
Size: 197 926 351 63 1232 901 262 1553
Fragments arranged by size:
1553 1232 926 901 351 262 197 63
SnaBI TAC'GTA
Cuts at: 493 3276 493
Size: 2783 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5485
SphI G_CATG'C
Cuts at: 1132 2843 1132
Size: 1711 3774
SspI AAT'ATT
Cuts at: 5 52 3409 5
Size: 47 3357 2081
Fragments arranged by size:
3357 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5485
StyI C'CwwG_G
Cuts at: 513 1065 2924 513
Size: 552 1859 3074
Fragments arranged by size:
3074 1859 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3512 3529 3682 3867 4312 325
Size: 1255 1932 17 153 185 445 1498
Fragments arranged by size:
1932 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3731 5443 96
Size: 274 80 33 51 1443 1754 1712 138
Fragments arranged by size:
1754 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2558 2590 5445 5477 2558
Size: 32 2855 32 2566
Fragments arranged by size:
2855 2566 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5485
XbaI T'CTAG_A
Cuts at: 2821 2821
Size: 5485
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2548 2548
Size: 5485
XhoI C'TCGA_G
Cuts at: 1943 2294 1943
Size: 351 5134
XmaI C'CCGG_G
Cuts at: 1870 2375 2441 2682 2810 1870
Size: 505 66 241 128 4545
Fragments arranged by size:
4545 505 241 128 66
XmnI GAAnn'nnTTC
Cuts at: 3614 3614
Size: 5485
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3293 276
Size: 53 83 186 936 1759 2468
Fragments arranged by size:
2468 1759 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PspXI PstI
PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI
FspAI NruI NsiI PacI PfoI PmlI PpuMI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SrfI SwaI