Restriction map for
pFN21AB8909
(Circular) (Six-base) MAPSORT of: pFN21AB8909.seq Check: 3415 from: 1 to: 5437
pFN21AB8909 5437 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3247 278
Size: 53 83 186 936 1711 2468
Fragments arranged by size:
2468 1711 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2780 1937
Size: 843 4594
Acc65I G'GTAC_C
Cuts at: 2758 2758
Size: 5437
AclI AA'CG_TT
Cuts at: 1563 3564 3937 1563
Size: 2001 373 3063
Fragments arranged by size:
3063 2001 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3500 4884 5152 1632
Size: 1868 1384 268 1917
Fragments arranged by size:
1917 1868 1384 268
AfeI AGC'GCT
Cuts at: 2636 2636
Size: 5437
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5240
AflIII A'CryG_T
Cuts at: 1203 4336 5313 1203
Size: 3133 977 1327
Fragments arranged by size:
3133 1327 977
AgeI A'CCGG_T
Cuts at: 4268 4268
Size: 5437
AhdI GACnn_n'nnGTC
Cuts at: 4166 4166
Size: 5437
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5437
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5437
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2594 2626 2594
Size: 32 5405
AlwNI CAG_nnn'CTG
Cuts at: 2047 2451 3180 4752 2047
Size: 404 729 1572 2732
Fragments arranged by size:
2732 1572 729 404
ApaLI G'TGCA_C
Cuts at: 3494 4650 3494
Size: 1156 4281
ApoI r'AATT_y
Cuts at: 1493 2746 2972 3124 5233 1493
Size: 1253 226 152 2109 1697
Fragments arranged by size:
2109 1697 1253 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3991 160
Size: 872 2959 1606
Fragments arranged by size:
2959 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5437
AvaI C'yCGr_G
Cuts at: 1870 1943 2152 2762 1870
Size: 73 209 610 4545
Fragments arranged by size:
4545 610 209 73
BamHI G'GATC_C
Cuts at: 2285 2350 2767 3134 2285
Size: 65 417 367 4588
Fragments arranged by size:
4588 417 367 65
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2074 2758 4213 618
Size: 325 217 627 287 684 1455 1842
Fragments arranged by size:
1842 1455 684 627 325 287 217
BanII G_rGCy'C
Cuts at: 1391 2756 1391
Size: 1365 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4466
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3628 3662 1903
Size: 34 1691 34 3678
Fragments arranged by size:
3678 1691 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3329 4539 3329
Size: 1210 4227
BclI T'GATC_A
Cuts at: 1548 2700 1548
Size: 1152 4285
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4048 136
Size: 107 122 71 631 605 2376 1525
Fragments arranged by size:
2376 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5432 5432
Size: 5437
BlpI GC'TnA_GC
Cuts at: 2854 2854
Size: 5437
Bme1580I G_kGCm'C
Cuts at: 1790 2441 3498 4654 1790
Size: 651 1057 1156 2573
Fragments arranged by size:
2573 1156 1057 651
BmgBI CAC'GTC
Cuts at: 1318 2299 1318
Size: 981 4456
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2035 2607 3168
Size: 416 53 83 399 24 618 572 561
Cuts at: 3168 4126 442
Size: 958 1753
Fragments arranged by size:
1753 958 618 572 561 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5437
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2412 4097 1239
Size: 1173 1685 2579
Fragments arranged by size:
2579 1685 1173
Bpu10I CC'TnA_GC
Cuts at: 2121 2121
Size: 5437
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3562 4427 4725 3562
Size: 865 298 4274
Fragments arranged by size:
4274 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4100 882
Size: 617 2601 2219
Fragments arranged by size:
2601 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2532 3228 493
Size: 711 1328 696 2702
Fragments arranged by size:
2702 1328 711 696
BsaBI GATnn'nnATC
Cuts at: 5431 5431
Size: 5437
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2100 3244 3626 4261
Size: 53 83 186 936 567 1144 382 635
Cuts at: 4261 275
Size: 1451
Fragments arranged by size:
1451 1144 936 635 567 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2095 2586 3870 4268 4542 4689 5100 2095
Size: 491 1284 398 274 147 411 2432
Fragments arranged by size:
2432 1284 491 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5212
Fragments arranged by size:
5212 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2354 2372 2659 1186
Size: 1168 18 287 3964
Fragments arranged by size:
3964 1168 287 18
BseYI C'CCAG_C
Cuts at: 2366 2693 4640 5136 2366
Size: 327 1947 496 2667
Fragments arranged by size:
2667 1947 496 327
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2161 2269 2512 1163
Size: 573 425 108 243 4088
Fragments arranged by size:
4088 573 425 243 108
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2016 2711 2887 3648 4676 1540
Size: 275 186 15 695 176 761 1028 2301
Fragments arranged by size:
2301 1028 761 695 275 186 176 15
BsiHKAI G_wGCw'C
Cuts at: 2145 3498 3583 4654 2145
Size: 1353 85 1071 2928
Fragments arranged by size:
2928 1353 1071 85
BsmI GAATG_Cn'
Cuts at: 2948 3041 2948
Size: 93 5344
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2145 2441 2756 3498 3583 4654 1391
Size: 399 355 296 315 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 355 315 296 85
BspEI T'CCGG_A
Cuts at: 2095 5100 2095
Size: 3005 2432
BspHI T'CATG_A
Cuts at: 2640 3326 5056 2640
Size: 686 1730 3021
Fragments arranged by size:
3021 1730 686
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2792 844
Size: 1948 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2107 2374 2562 3324 1394
Size: 429 284 267 188 762 3507
Fragments arranged by size:
3507 762 429 284 267 188
BsrDI GCAATG_nn'
Cuts at: 65 1248 3932 4106 65
Size: 1183 2684 174 1396
Fragments arranged by size:
2684 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4081 4268 1700
Size: 110 2271 187 2869
Fragments arranged by size:
2869 2271 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5437
BssHII G'CGCG_C
Cuts at: 1925 2361 1925
Size: 436 5001
BssSI C'ACGA_G
Cuts at: 3497 4509 3497
Size: 1012 4425
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5437
BstBI TT'CG_AA
Cuts at: 2060 4241 2060
Size: 2181 3256
BstEII G'GTnAC_C
Cuts at: 1184 2131 2147 2185 1184
Size: 947 16 38 4436
Fragments arranged by size:
4436 947 38 16
BstXI CCAn_nnnn'nTGG
Cuts at: 2734 2734
Size: 5437
BstYI r'GATC_y
Cuts at: 1972 2285 2350 2767 3134 3519 3536 4988 5074
Size: 313 65 417 367 385 17 1452 86
Cuts at: 5074 5086 5432 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 417 385 367 346 313 86
65 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2010 513
Size: 552 945 3940
Fragments arranged by size:
3940 945 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2176 487
Size: 683 56 950 3748
Fragments arranged by size:
3748 950 683 56
BtsI GCAGTG_nn'
Cuts at: 2953 3747 3767 2953
Size: 794 20 4623
Fragments arranged by size:
4623 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5437
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5402
DraI TTT'AAA
Cuts at: 2741 2741
Size: 5437
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4444 809
Size: 507 3128 1802
Fragments arranged by size:
3128 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2013 2534 2724
Size: 54 1425 50 275 18 183 521 190
Cuts at: 2724 2884 3773 5402 8
Size: 160 889 1629 43
Fragments arranged by size:
1629 1425 889 521 275 190 183 160
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2013 2884 1537
Size: 275 201 871 4090
Fragments arranged by size:
4090 871 275 201
EarI CTCTTCn'nnn_
Cuts at: 1353 3367 1353
Size: 2014 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4024 4398 4544 387
Size: 1064 138 2435 374 146 1280
Fragments arranged by size:
2435 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2412 3500 4097 4884 5152 1239
Size: 393 780 1088 597 787 268 1524
Fragments arranged by size:
1524 1088 787 780 597 393 268
EcoRI G'AATT_C
Cuts at: 2746 3124 2746
Size: 378 5059
FspI TGC'GCA
Cuts at: 3943 3943
Size: 5437
HaeII r_GCGC'y
Cuts at: 1678 2638 2723 4584 1678
Size: 960 85 1861 2531
Fragments arranged by size:
2531 1861 960 85
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2270 2302 2335 2367 4078
Size: 32 135 32 698 32 33 32 1711
Cuts at: 4078 4110 4152 4184 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1711 698 135 42 33 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2781 3027 669
Size: 988 281 843 246 3079
Fragments arranged by size:
3079 988 843 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5437
HpaI GTT'AAC
Cuts at: 3027 3027
Size: 5437
KpnI G_GTAC'C
Cuts at: 2762 2762
Size: 5437
MfeI C'AATT_G
Cuts at: 3036 3036
Size: 5437
MluI A'CGCG_T
Cuts at: 5313 5313
Size: 5437
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2187 2313 4551 4735 1305
Size: 882 126 2238 184 2007
Fragments arranged by size:
2238 2007 882 184 126
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2536 2726 10
Size: 54 1425 343 704 190 2721
Fragments arranged by size:
2721 1425 704 343 190 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2508 3395 3754 3913 518
Size: 648 1342 887 359 159 2042
Fragments arranged by size:
2042 1342 887 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2012 2616 2623 2853 3530 4678 4923 1606
Size: 406 604 7 230 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 604 406 245 230 7
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4885
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5437
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5437
NotI GC'GGCC_GC
Cuts at: 2013 2884 2013
Size: 871 4566
NspI r_CATG'y
Cuts at: 1132 1229 2795 4340 1132
Size: 97 1566 1545 2229
Fragments arranged by size:
2229 1566 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5437
PciI A'CATG_T
Cuts at: 4336 4336
Size: 5437
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2047 1108
Size: 939 4498
PfoI T'CCnGG_A
Cuts at: 2389 2389
Size: 5437
PmeI GTTT'AAAC
Cuts at: 2741 2741
Size: 5437
PmlI CAC'GTG
Cuts at: 2532 2532
Size: 5437
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3506 3538 5019 5051 3506
Size: 32 1481 32 3892
Fragments arranged by size:
3892 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5437
PsiI TTA'TAA
Cuts at: 3007 3007
Size: 5437
PstI C_TGCA'G
Cuts at: 830 2789 830
Size: 1959 3478
PvuI CG_AT'CG
Cuts at: 2001 2711 2001
Size: 710 4727
SacII CC_GC'GG
Cuts at: 2013 2013
Size: 5437
SalI G'TCGA_C
Cuts at: 1936 2779 1936
Size: 843 4594
SbfI CC_TGCA'GG
Cuts at: 2789 2789
Size: 5437
ScaI AGT'ACT
Cuts at: 3685 5397 3685
Size: 1712 3725
SfcI C'TryA_G
Cuts at: 826 1045 2443 2785 3920 4601 4792 826
Size: 219 1398 342 1135 681 191 1471
Fragments arranged by size:
1471 1398 1135 681 342 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5437
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5437
SmaI CCC'GGG
Cuts at: 1872 2154 2764 1872
Size: 282 610 4545
Fragments arranged by size:
4545 610 282
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3541 4442 4704 820
Size: 197 926 1598 901 262 1553
Fragments arranged by size:
1598 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3228 493
Size: 2735 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5437
SphI G_CATG'C
Cuts at: 1132 2795 1132
Size: 1663 3774
SspI AAT'ATT
Cuts at: 5 52 3361 5
Size: 47 3309 2081
Fragments arranged by size:
3309 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5437
StyI C'CwwG_G
Cuts at: 513 1065 2136 2876 513
Size: 552 1071 740 3074
Fragments arranged by size:
3074 1071 740 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3464 3481 3634 3819 4264 325
Size: 1255 1884 17 153 185 445 1498
Fragments arranged by size:
1884 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3683 5395 96
Size: 274 80 33 51 1443 1706 1712 138
Fragments arranged by size:
1712 1706 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5397 5429 5397
Size: 32 5405
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5437
XbaI T'CTAG_A
Cuts at: 2773 2773
Size: 5437
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2203 2203
Size: 5437
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5437
XmaI C'CCGG_G
Cuts at: 1870 2152 2762 1870
Size: 282 610 4545
Fragments arranged by size:
4545 610 282
XmnI GAAnn'nnTTC
Cuts at: 2636 3566 2636
Size: 930 4507
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3245 276
Size: 53 83 186 936 1711 2468
Fragments arranged by size:
2468 1711 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI
PvuI SacII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SanDI SapI SexAI SfiI SfoI
SrfI SwaI