Restriction map for
pFN21AB8908
(Circular) (Six-base) MAPSORT of: pFN21AB8908.seq Check: 8687 from: 1 to: 5431
pFN21AB8908 5431 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3241 278
Size: 53 83 186 936 1705 2468
Fragments arranged by size:
2468 1705 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2774 1937
Size: 837 4594
Acc65I G'GTAC_C
Cuts at: 2752 2752
Size: 5431
AclI AA'CG_TT
Cuts at: 1563 3558 3931 1563
Size: 1995 373 3063
Fragments arranged by size:
3063 1995 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3494 4878 5146 1632
Size: 1862 1384 268 1917
Fragments arranged by size:
1917 1862 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5234
AflIII A'CryG_T
Cuts at: 1203 4330 5307 1203
Size: 3127 977 1327
Fragments arranged by size:
3127 1327 977
AgeI A'CCGG_T
Cuts at: 4262 4262
Size: 5431
AhdI GACnn_n'nnGTC
Cuts at: 4160 4160
Size: 5431
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5431
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5431
AlwNI CAG_nnn'CTG
Cuts at: 3174 4746 3174
Size: 1572 3859
ApaI G_GGCC'C
Cuts at: 2130 2130
Size: 5431
ApaLI G'TGCA_C
Cuts at: 3488 4644 3488
Size: 1156 4275
ApoI r'AATT_y
Cuts at: 1493 2264 2740 2966 3118 5227 1493
Size: 771 476 226 152 2109 1697
Fragments arranged by size:
2109 1697 771 476 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3985 160
Size: 872 2953 1606
Fragments arranged by size:
2953 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5431
AvaI C'yCGr_G
Cuts at: 1870 1943 2652 2756 1870
Size: 73 709 104 4545
Fragments arranged by size:
4545 709 104 73
BamHI G'GATC_C
Cuts at: 2761 3128 2761
Size: 367 5064
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2140 2192 2752 4207 618
Size: 325 217 627 353 52 560 1455 1842
Fragments arranged by size:
1842 1455 627 560 353 325 217 52
BanII G_rGCy'C
Cuts at: 1391 2012 2130 2311 2653 2750 1391
Size: 621 118 181 342 97 4072
Fragments arranged by size:
4072 621 342 181 118 97
BbeI G_GCGC'C
Cuts at: 2144 2144
Size: 5431
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4460
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2311 2345 3622 3656 1903
Size: 34 374 34 1277 34 3678
Fragments arranged by size:
3678 1277 374 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3323 4533 3323
Size: 1210 4221
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5431
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4042 136
Size: 107 122 71 631 605 2370 1525
Fragments arranged by size:
2370 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2586 5426 2586
Size: 2840 2591
BlpI GC'TnA_GC
Cuts at: 2240 2310 2848 2240
Size: 70 538 4823
Fragments arranged by size:
4823 538 70
Bme1580I G_kGCm'C
Cuts at: 1790 2130 3492 4648 1790
Size: 340 1362 1156 2573
Fragments arranged by size:
2573 1362 1156 340
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5431
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3162 4120 442
Size: 416 53 83 399 24 1745 958 1753
Fragments arranged by size:
1753 1745 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5431
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4091 1239
Size: 2852 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3556 4421 4719 3556
Size: 865 298 4268
Fragments arranged by size:
4268 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2425 4094 882
Size: 617 926 1669 2219
Fragments arranged by size:
2219 1669 926 617
BsaAI yAC'GTr
Cuts at: 493 1204 3222 493
Size: 711 2018 2702
Fragments arranged by size:
2702 2018 711
BsaBI GATnn'nnATC
Cuts at: 5425 5425
Size: 5431
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2141 2256 3238 3620
Size: 53 83 186 936 608 115 982 382
Cuts at: 3620 4255 275
Size: 635 1451
Fragments arranged by size:
1451 982 936 635 608 382 186 115
83 53
BsaWI w'CCGG_w
Cuts at: 3864 4262 4536 4683 5094 3864
Size: 398 274 147 411 4201
Fragments arranged by size:
4201 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2180 2210 2440 2470 1178
Size: 30 165 30 777 30 230 30 4139
Fragments arranged by size:
4139 777 230 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2675 2708 2720 1186
Size: 1489 33 12 3897
Fragments arranged by size:
3897 1489 33 12
BseYI C'CCAG_C
Cuts at: 2408 4634 5130 2408
Size: 2226 496 2709
Fragments arranged by size:
2709 2226 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4858
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2881 3642 4670 1540
Size: 275 186 880 761 1028 2301
Fragments arranged by size:
2301 1028 880 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2012 2237 3492 3577 4648 2012
Size: 225 1255 85 1071 2795
Fragments arranged by size:
2795 1255 1071 225 85
BsmI GAATG_Cn'
Cuts at: 2942 3035 2942
Size: 93 5338
BsmBI CGTCTCn'nnnn_
Cuts at: 2247 2409 2247
Size: 162 5269
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2130 2237 2311 2653 2750 3492
Size: 399 222 118 107 74 342 97 742
Cuts at: 3492 3577 4648 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 342 222 118 107
97 85 74
BspEI T'CCGG_A
Cuts at: 5094 5094
Size: 5431
BspHI T'CATG_A
Cuts at: 3320 5050 3320
Size: 1730 3701
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2786 844
Size: 1942 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3318 1394
Size: 429 1495 3507
Fragments arranged by size:
3507 1495 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3926 4100 65
Size: 1183 2678 174 1396
Fragments arranged by size:
2678 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2048 2065 2082 2475 4075 4262 1700
Size: 110 238 17 17 393 1600 187 2869
Fragments arranged by size:
2869 1600 393 238 187 110 17 17
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5431
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5431
BssSI C'ACGA_G
Cuts at: 3491 4503 3491
Size: 1012 4419
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5431
BstBI TT'CG_AA
Cuts at: 4235 4235
Size: 5431
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5431
BstYI r'GATC_y
Cuts at: 1972 2586 2761 3128 3513 3530 4982 5068 5080
Size: 614 175 367 385 17 1452 86 12
Cuts at: 5080 5426 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 614 385 367 346 175 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2188 2248 2423 513
Size: 552 1123 60 175 3521
Fragments arranged by size:
3521 1123 552 175 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4692
Fragments arranged by size:
4692 683 56
BtsI GCAGTG_nn'
Cuts at: 2461 2947 3741 3761 2461
Size: 486 794 20 4131
Fragments arranged by size:
4131 794 486 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5431
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5396
DraI TTT'AAA
Cuts at: 2735 2735
Size: 5431
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4438 809
Size: 507 3122 1802
Fragments arranged by size:
3122 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2878 3767 5396
Size: 54 1425 50 275 18 1048 889 1629
Cuts at: 5396 8
Size: 43
Fragments arranged by size:
1629 1425 1048 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2878 1537
Size: 275 1066 4090
Fragments arranged by size:
4090 1066 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2685 2697 3361 1353
Size: 1332 12 664 3423
Fragments arranged by size:
3423 1332 664 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2344 4018 4392 4538 387
Size: 1064 138 755 1674 374 146 1280
Fragments arranged by size:
1674 1280 1064 755 374 146 138
EcoICRI GAG'CTC
Cuts at: 2010 2010
Size: 5431
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3494 4091 4878 5146 1239
Size: 393 1862 597 787 268 1524
Fragments arranged by size:
1862 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2740 3118 2740
Size: 378 5053
FspI TGC'GCA
Cuts at: 3937 3937
Size: 5431
HaeII r_GCGC'y
Cuts at: 1678 2144 2285 4578 1678
Size: 466 141 2293 2531
Fragments arranged by size:
2531 2293 466 141
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2333 2365 4072 4104 4146
Size: 32 135 32 761 32 1707 32 42
Cuts at: 4146 4178 1373
Size: 32 2626
Fragments arranged by size:
2626 1707 761 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2023 2775 3021 669
Size: 988 281 85 752 246 3079
Fragments arranged by size:
3079 988 752 281 246 85
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5431
HpaI GTT'AAC
Cuts at: 3021 3021
Size: 5431
KasI G'GCGC_C
Cuts at: 2140 2140
Size: 5431
KpnI G_GTAC'C
Cuts at: 2756 2756
Size: 5431
MfeI C'AATT_G
Cuts at: 3030 3030
Size: 5431
MluI A'CGCG_T
Cuts at: 5307 5307
Size: 5431
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2232 2519 4545 4729 1305
Size: 927 287 2026 184 2007
Fragments arranged by size:
2026 2007 927 287 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3609
Fragments arranged by size:
3609 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3389 3748 3907 518
Size: 648 2223 359 159 2042
Fragments arranged by size:
2223 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2847 3524 4672 4917 1606
Size: 1241 677 1148 245 2120
Fragments arranged by size:
2120 1241 1148 677 245
NaeI GCC'GGC
Cuts at: 2050 2050
Size: 5431
NarI GG'CG_CC
Cuts at: 2141 2141
Size: 5431
NcoI C'CATG_G
Cuts at: 513 1065 2423 513
Size: 552 1358 3521
Fragments arranged by size:
3521 1358 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5431
NgoMIV G'CCGG_C
Cuts at: 2048 2048
Size: 5431
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5431
NotI GC'GGCC_GC
Cuts at: 2878 2878
Size: 5431
NspI r_CATG'y
Cuts at: 1132 1229 2789 4334 1132
Size: 97 1560 1545 2229
Fragments arranged by size:
2229 1560 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2560 1348
Size: 1212 4219
PciI A'CATG_T
Cuts at: 4330 4330
Size: 5431
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2116 1108
Size: 1008 4423
PmeI GTTT'AAAC
Cuts at: 2735 2735
Size: 5431
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2219 2251 3500 3532 5013 5045 2219
Size: 32 1249 32 1481 32 2605
Fragments arranged by size:
2605 1481 1249 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5431
PsiI TTA'TAA
Cuts at: 3001 3001
Size: 5431
PspOMI G'GGCC_C
Cuts at: 2126 2126
Size: 5431
PstI C_TGCA'G
Cuts at: 830 2783 830
Size: 1953 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5431
SacI G_AGCT'C
Cuts at: 2012 2012
Size: 5431
SalI G'TCGA_C
Cuts at: 1936 2773 1936
Size: 837 4594
SbfI CC_TGCA'GG
Cuts at: 2783 2783
Size: 5431
ScaI AGT'ACT
Cuts at: 3679 5391 3679
Size: 1712 3719
SfcI C'TryA_G
Cuts at: 826 1045 2779 3914 4595 4786 826
Size: 219 1734 1135 681 191 1471
Fragments arranged by size:
1734 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2142 2142
Size: 5431
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5431
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5431
SmaI CCC'GGG
Cuts at: 1872 2758 1872
Size: 886 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3535 4436 4698 820
Size: 197 926 1592 901 262 1553
Fragments arranged by size:
1592 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3222 493
Size: 2729 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5431
SphI G_CATG'C
Cuts at: 1132 2789 1132
Size: 1657 3774
SspI AAT'ATT
Cuts at: 5 52 3355 5
Size: 47 3303 2081
Fragments arranged by size:
3303 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5431
StyI C'CwwG_G
Cuts at: 513 1065 2423 2487 2870 513
Size: 552 1358 64 383 3074
Fragments arranged by size:
3074 1358 552 383 64
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2585 3458 3475 3628 3813 4258 325
Size: 1255 1005 873 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1005 873 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3677 5389 96
Size: 274 80 33 51 1443 1700 1712 138
Fragments arranged by size:
1712 1700 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2180 2212 2440 2472 5391 5423 2180
Size: 32 228 32 2919 32 2188
Fragments arranged by size:
2919 2188 228 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5431
XbaI T'CTAG_A
Cuts at: 2767 2767
Size: 5431
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5431
XmaI C'CCGG_G
Cuts at: 1870 2756 1870
Size: 886 4545
XmnI GAAnn'nnTTC
Cuts at: 3560 3560
Size: 5431
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3239 276
Size: 53 83 186 936 1705 2468
Fragments arranged by size:
2468 1705 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI
EcoO109I EcoRV FalI FseI FspAI NruI NsiI PacI
PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacII
SanDI SapI SexAI SfiI SrfI SwaI XcmI