Restriction map for
pFN21AB8905
(Circular) (Six-base) MAPSORT of: pFN21AB8905.seq Check: 2360 from: 1 to: 5416
pFN21AB8905 5416 bp
With 182 enzymes: SgfI *
October 20, 2009 10:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3226 278
Size: 53 83 186 936 1690 2468
Fragments arranged by size:
2468 1690 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2759 1937
Size: 822 4594
Acc65I G'GTAC_C
Cuts at: 2737 2737
Size: 5416
AclI AA'CG_TT
Cuts at: 1563 3543 3916 1563
Size: 1980 373 3063
Fragments arranged by size:
3063 1980 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2146 2301 2504 2525 3479 4863 5131 1632
Size: 514 155 203 21 954 1384 268 1917
Fragments arranged by size:
1917 1384 954 514 268 203 155 21
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5219
AflIII A'CryG_T
Cuts at: 1203 4315 5292 1203
Size: 3112 977 1327
Fragments arranged by size:
3112 1327 977
AgeI A'CCGG_T
Cuts at: 4247 4247
Size: 5416
AhdI GACnn_n'nnGTC
Cuts at: 4145 4145
Size: 5416
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5416
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5416
AlwNI CAG_nnn'CTG
Cuts at: 2248 2321 3159 4731 2248
Size: 73 838 1572 2933
Fragments arranged by size:
2933 1572 838 73
ApaI G_GGCC'C
Cuts at: 2315 2334 2315
Size: 19 5397
ApaLI G'TGCA_C
Cuts at: 3473 4629 3473
Size: 1156 4260
ApoI r'AATT_y
Cuts at: 1493 2725 2951 3103 5212 1493
Size: 1232 226 152 2109 1697
Fragments arranged by size:
2109 1697 1232 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3970 160
Size: 872 2938 1606
Fragments arranged by size:
2938 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5416
AvaI C'yCGr_G
Cuts at: 1870 1943 2631 2674 2684 2741 1870
Size: 73 688 43 10 57 4545
Fragments arranged by size:
4545 688 73 57 43 10
BamHI G'GATC_C
Cuts at: 2746 3113 2746
Size: 367 5049
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2195 2493 2737 4192 618
Size: 325 217 627 408 298 244 1455 1842
Fragments arranged by size:
1842 1455 627 408 325 298 244 217
BanII G_rGCy'C
Cuts at: 1391 2126 2315 2334 2357 2374 2735 1391
Size: 735 189 19 23 17 361 4072
Fragments arranged by size:
4072 735 361 189 23 19 17
BbeI G_GCGC'C
Cuts at: 2497 2497
Size: 5416
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4445
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2410 2444 2513 2547 3607 3641 1903
Size: 34 473 34 69 34 1060 34 3678
Fragments arranged by size:
3678 1060 473 69 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3308 4518 3308
Size: 1210 4206
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5416
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2100 2585 2618
Size: 107 122 71 631 605 428 485 33
Cuts at: 2618 2627 4027 136
Size: 9 1400 1525
Fragments arranged by size:
1525 1400 631 605 485 428 122 107
71 33 9
BglII A'GATC_T
Cuts at: 5411 5411
Size: 5416
BlpI GC'TnA_GC
Cuts at: 2833 2833
Size: 5416
Bme1580I G_kGCm'C
Cuts at: 1790 2198 2315 2334 3477 4633 1790
Size: 408 117 19 1143 1156 2573
Fragments arranged by size:
2573 1156 1143 408 117 19
BmgBI CAC'GTC
Cuts at: 1318 2380 1318
Size: 1062 4354
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2129 2309 3147
Size: 416 53 83 399 24 712 180 838
Cuts at: 3147 4105 442
Size: 958 1753
Fragments arranged by size:
1753 958 838 712 416 399 180 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5416
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2231 2585 4076 1239
Size: 992 354 1491 2579
Fragments arranged by size:
2579 1491 992 354
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3541 4406 4704 3541
Size: 865 298 4253
Fragments arranged by size:
4253 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2422 4079 882
Size: 617 923 1657 2219
Fragments arranged by size:
2219 1657 923 617
BsaAI yAC'GTr
Cuts at: 493 1204 3207 493
Size: 711 2003 2702
Fragments arranged by size:
2702 2003 711
BsaBI GATnn'nnATC
Cuts at: 5410 5410
Size: 5416
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2494 3223 3605 4240
Size: 53 83 186 936 961 729 382 635
Cuts at: 4240 275
Size: 1451
Fragments arranged by size:
1451 961 936 729 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3849 4247 4521 4668 5079 3849
Size: 398 274 147 411 4186
Fragments arranged by size:
4186 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2204 2234 1178
Size: 30 165 30 801 30 4360
Fragments arranged by size:
4360 801 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2228 2375 1186
Size: 1042 147 4227
Fragments arranged by size:
4227 1042 147
BseYI C'CCAG_C
Cuts at: 2018 2248 2255 2373 2592 4619 5115 2018
Size: 230 7 118 219 2027 496 2319
Fragments arranged by size:
2319 2027 496 230 219 118 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4843
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2866 3627 4655 1540
Size: 275 186 865 761 1028 2301
Fragments arranged by size:
2301 1028 865 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3477 3562 4633 3477
Size: 85 1071 4260
Fragments arranged by size:
4260 1071 85
BsmI GAATG_Cn'
Cuts at: 2927 3020 2927
Size: 93 5323
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2126 2198 2315 2334 2357 2374 2735
Size: 399 336 72 117 19 23 17 361
Cuts at: 2735 3477 3562 4633 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 361 336 117 85
72 23 19 17
BspEI T'CCGG_A
Cuts at: 5079 5079
Size: 5416
BspHI T'CATG_A
Cuts at: 3305 5035 3305
Size: 1730 3686
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2771 844
Size: 1927 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2395 2679 3303 1394
Size: 429 572 284 624 3507
Fragments arranged by size:
3507 624 572 429 284
BsrDI GCAATG_nn'
Cuts at: 65 1248 3911 4085 65
Size: 1183 2663 174 1396
Fragments arranged by size:
2663 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2442 2664 4060 4247 1700
Size: 110 632 222 1396 187 2869
Fragments arranged by size:
2869 1396 632 222 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5416
BssHII G'CGCG_C
Cuts at: 1925 2575 1925
Size: 650 4766
BssSI C'ACGA_G
Cuts at: 3476 4488 3476
Size: 1012 4404
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5416
BstBI TT'CG_AA
Cuts at: 4220 4220
Size: 5416
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5416
BstXI CCAn_nnnn'nTGG
Cuts at: 2026 2026
Size: 5416
BstYI r'GATC_y
Cuts at: 1972 2746 3113 3498 3515 4967 5053 5065 5411
Size: 774 367 385 17 1452 86 12 346
Cuts at: 5411 1972
Size: 1977
Fragments arranged by size:
1977 1452 774 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2031 2289 2335 2512 513
Size: 552 939 27 258 46 177 3417
Fragments arranged by size:
3417 939 552 258 177 46 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4677
Fragments arranged by size:
4677 683 56
BtsI GCAGTG_nn'
Cuts at: 2932 3726 3746 2932
Size: 794 20 4602
Fragments arranged by size:
4602 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5416
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5381
DraI TTT'AAA
Cuts at: 2720 2720
Size: 5416
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4423 809
Size: 507 3107 1802
Fragments arranged by size:
3107 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2028 2201 2586
Size: 54 1425 50 275 18 198 173 385
Cuts at: 2586 2863 3752 5381 8
Size: 277 889 1629 43
Fragments arranged by size:
1629 1425 889 385 277 275 198 173
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2863 1537
Size: 275 1051 4090
Fragments arranged by size:
4090 1051 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2277 2714 3346 1353
Size: 924 437 632 3423
Fragments arranged by size:
3423 924 632 437
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4003 4377 4523 387
Size: 1064 138 2414 374 146 1280
Fragments arranged by size:
2414 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2146 2231 2301 2504 2525 2585 3479
Size: 393 514 85 70 203 21 60 894
Cuts at: 3479 4076 4863 5131 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 894 787 597 514 393 268 203
85 70 60 21
EcoO109I rG'GnC_Cy
Cuts at: 2311 2331 2695 2311
Size: 20 364 5032
Fragments arranged by size:
5032 364 20
EcoRI G'AATT_C
Cuts at: 2725 3103 2725
Size: 378 5038
FspI TGC'GCA
Cuts at: 3922 3922
Size: 5416
HaeII r_GCGC'y
Cuts at: 1678 2414 2477 2497 4563 1678
Size: 736 63 20 2066 2531
Fragments arranged by size:
2531 2066 736 63 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4057 4089 4131 4163 1373
Size: 32 135 32 2485 32 42 32 2626
Fragments arranged by size:
2626 2485 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2760 3006 669
Size: 988 281 822 246 3079
Fragments arranged by size:
3079 988 822 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5416
HpaI GTT'AAC
Cuts at: 3006 3006
Size: 5416
KasI G'GCGC_C
Cuts at: 2493 2493
Size: 5416
KpnI G_GTAC'C
Cuts at: 2741 2741
Size: 5416
MfeI C'AATT_G
Cuts at: 3015 3015
Size: 5416
MluI A'CGCG_T
Cuts at: 5292 5292
Size: 5416
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2427 4530 4714 1305
Size: 1122 2103 184 2007
Fragments arranged by size:
2103 2007 1122 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2030 10
Size: 54 1425 343 198 3396
Fragments arranged by size:
3396 1425 343 198 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3374 3733 3892 518
Size: 648 2208 359 159 2042
Fragments arranged by size:
2208 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2248 2386 2449 2514 2554 2596 2832 3509
Size: 642 138 63 65 40 42 236 677
Cuts at: 3509 4657 4902 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 642 245 236 138 65
63 42 40
NarI GG'CG_CC
Cuts at: 2494 2494
Size: 5416
NcoI C'CATG_G
Cuts at: 513 1065 2004 2031 2289 2335 513
Size: 552 939 27 258 46 3594
Fragments arranged by size:
3594 939 552 258 46 27
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5416
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5416
NotI GC'GGCC_GC
Cuts at: 2863 2863
Size: 5416
NspI r_CATG'y
Cuts at: 1132 1229 2774 4319 1132
Size: 97 1545 1545 2229
Fragments arranged by size:
2229 1545 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2348 1348
Size: 1000 4416
PciI A'CATG_T
Cuts at: 4315 4315
Size: 5416
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5416
PmeI GTTT'AAAC
Cuts at: 2720 2720
Size: 5416
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3485 3517 4998 5030 3485
Size: 32 1481 32 3871
Fragments arranged by size:
3871 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2695 2695
Size: 5416
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5416
PsiI TTA'TAA
Cuts at: 2986 2986
Size: 5416
PspOMI G'GGCC_C
Cuts at: 2311 2330 2311
Size: 19 5397
PspXI vC'TCGA_Gb
Cuts at: 2674 2674
Size: 5416
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2501 2533 2501
Size: 32 5384
PstI C_TGCA'G
Cuts at: 830 2241 2304 2550 2768 830
Size: 1411 63 246 218 3478
Fragments arranged by size:
3478 1411 246 218 63
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5416
PvuII CAG'CTG
Cuts at: 2248 2386 2449 2596 2248
Size: 138 63 147 5068
Fragments arranged by size:
5068 147 138 63
SacII CC_GC'GG
Cuts at: 2515 2515
Size: 5416
SalI G'TCGA_C
Cuts at: 1936 2758 1936
Size: 822 4594
SanDI GG'GwC_CC
Cuts at: 2695 2695
Size: 5416
SbfI CC_TGCA'GG
Cuts at: 2768 2768
Size: 5416
ScaI AGT'ACT
Cuts at: 3664 5376 3664
Size: 1712 3704
SfcI C'TryA_G
Cuts at: 826 1045 2109 2237 2300 2546 2764 3899 4580
Size: 219 1064 128 63 246 218 1135 681
Cuts at: 4580 4771 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1064 681 246 219 218 191
128 63
SfiI GGCCn_nnn'nGGCC
Cuts at: 2100 2618 2627 2100
Size: 518 9 4889
Fragments arranged by size:
4889 518 9
SfoI GGC'GCC
Cuts at: 2495 2495
Size: 5416
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5416
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5416
SmaI CCC'GGG
Cuts at: 1872 2633 2686 2743 1872
Size: 761 53 57 4545
Fragments arranged by size:
4545 761 57 53
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2674 3520 4421 4683 820
Size: 197 926 731 846 901 262 1553
Fragments arranged by size:
1553 926 901 846 731 262 197
SnaBI TAC'GTA
Cuts at: 493 3207 493
Size: 2714 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5416
SphI G_CATG'C
Cuts at: 1132 2774 1132
Size: 1642 3774
SspI AAT'ATT
Cuts at: 5 52 3340 5
Size: 47 3288 2081
Fragments arranged by size:
3288 2081 47
StuI AGG'CCT
Cuts at: 1517 2208 2528 1517
Size: 691 320 4405
Fragments arranged by size:
4405 691 320
StyI C'CwwG_G
Cuts at: 513 1065 2004 2031 2289 2335 2855 513
Size: 552 939 27 258 46 520 3074
Fragments arranged by size:
3074 939 552 520 258 46 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2170 3443 3460 3613 3798 4243 325
Size: 1255 590 1273 17 153 185 445 1498
Fragments arranged by size:
1498 1273 1255 590 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2220 3662 5374
Size: 274 80 33 51 1443 243 1442 1712
Cuts at: 5374 96
Size: 138
Fragments arranged by size:
1712 1443 1442 274 243 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5376 5408 5376
Size: 32 5384
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5416
XbaI T'CTAG_A
Cuts at: 2752 2752
Size: 5416
XhoI C'TCGA_G
Cuts at: 1943 2674 1943
Size: 731 4685
XmaI C'CCGG_G
Cuts at: 1870 2631 2684 2741 1870
Size: 761 53 57 4545
Fragments arranged by size:
4545 761 57 53
XmnI GAAnn'nnTTC
Cuts at: 3545 3545
Size: 5416
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3224 276
Size: 53 83 186 936 1690 2468
Fragments arranged by size:
2468 1690 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII SacII SalI
SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PfoI PmlI RsrII SacI SapI SexAI
SrfI SwaI XcmI