Restriction map for
pFN21AB8902
(Circular) (Six-base) MAPSORT of: pFN21AB8902.seq Check: 1226 from: 1 to: 5407
pFN21AB8902 5407 bp
With 182 enzymes: SgfI *
October 20, 2009 10:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3217 278
Size: 53 83 186 936 1681 2468
Fragments arranged by size:
2468 1681 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2750 1937
Size: 813 4594
Acc65I G'GTAC_C
Cuts at: 2728 2728
Size: 5407
AclI AA'CG_TT
Cuts at: 1563 3534 3907 1563
Size: 1971 373 3063
Fragments arranged by size:
3063 1971 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2316 3470 4854 5122 1632
Size: 684 1154 1384 268 1917
Fragments arranged by size:
1917 1384 1154 684 268
AfeI AGC'GCT
Cuts at: 2381 2381
Size: 5407
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5210
AflIII A'CryG_T
Cuts at: 1203 4306 5283 1203
Size: 3103 977 1327
Fragments arranged by size:
3103 1327 977
AgeI A'CCGG_T
Cuts at: 4238 4238
Size: 5407
AhdI GACnn_n'nnGTC
Cuts at: 4136 4136
Size: 5407
AleI CACnn'nnGTG
Cuts at: 1166 2408 1166
Size: 1242 4165
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5407
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2414 2446 2414
Size: 32 5375
AlwNI CAG_nnn'CTG
Cuts at: 3150 4722 3150
Size: 1572 3835
ApaI G_GGCC'C
Cuts at: 2244 2470 2244
Size: 226 5181
ApaLI G'TGCA_C
Cuts at: 3464 4620 3464
Size: 1156 4251
ApoI r'AATT_y
Cuts at: 1493 2716 2942 3094 5203 1493
Size: 1223 226 152 2109 1697
Fragments arranged by size:
2109 1697 1223 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3961 160
Size: 872 2929 1606
Fragments arranged by size:
2929 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5407
AvaI C'yCGr_G
Cuts at: 1870 1943 2030 2385 2622 2732 1870
Size: 73 87 355 237 110 4545
Fragments arranged by size:
4545 355 237 110 87 73
BamHI G'GATC_C
Cuts at: 2737 3104 2737
Size: 367 5040
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2336 2728 4183 618
Size: 325 217 627 549 392 1455 1842
Fragments arranged by size:
1842 1455 627 549 392 325 217
BanII G_rGCy'C
Cuts at: 1391 2244 2470 2696 2726 1391
Size: 853 226 226 30 4072
Fragments arranged by size:
4072 853 226 226 30
BbeI G_GCGC'C
Cuts at: 2340 2340
Size: 5407
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4436
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3598 3632 1903
Size: 34 50 34 1577 34 3678
Fragments arranged by size:
3678 1577 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3299 4509 3299
Size: 1210 4197
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5407
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2293 2474 4018
Size: 107 122 71 631 605 621 181 1544
Cuts at: 4018 136
Size: 1525
Fragments arranged by size:
1544 1525 631 621 605 181 122 107
71
BglII A'GATC_T
Cuts at: 5402 5402
Size: 5407
BlpI GC'TnA_GC
Cuts at: 2231 2824 2231
Size: 593 4814
Bme1580I G_kGCm'C
Cuts at: 1790 2244 2470 3468 4624 1790
Size: 454 226 998 1156 2573
Fragments arranged by size:
2573 1156 998 454 226
BmgBI CAC'GTC
Cuts at: 1318 2085 1318
Size: 767 4640
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3138 4096 442
Size: 416 53 83 399 24 1721 958 1753
Fragments arranged by size:
1753 1721 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5407
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2602 2635 2602
Size: 33 5374
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2710 4067 1239
Size: 1471 1357 2579
Fragments arranged by size:
2579 1471 1357
Bpu10I CC'TnA_GC
Cuts at: 2009 2364 2009
Size: 355 5052
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2471 3532 4397 4695 2471
Size: 1061 865 298 3183
Fragments arranged by size:
3183 1061 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2273 2492 2641 4070 882
Size: 617 774 219 149 1429 2219
Fragments arranged by size:
2219 1429 774 617 219 149
BsaAI yAC'GTr
Cuts at: 493 1204 3198 493
Size: 711 1994 2702
Fragments arranged by size:
2702 1994 711
BsaBI GATnn'nnATC
Cuts at: 5401 5401
Size: 5407
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2337 3214 3596 4231
Size: 53 83 186 936 804 877 382 635
Cuts at: 4231 275
Size: 1451
Fragments arranged by size:
1451 936 877 804 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2017 3840 4238 4512 4659 5070 2017
Size: 1823 398 274 147 411 2354
Fragments arranged by size:
2354 1823 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2414 2444 1178
Size: 30 165 30 1011 30 4141
Fragments arranged by size:
4141 1011 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2273 2548 2578 2624 1186
Size: 1087 275 30 46 3969
Fragments arranged by size:
3969 1087 275 46 30
BseYI C'CCAG_C
Cuts at: 2566 2695 4610 5106 2566
Size: 129 1915 496 2867
Fragments arranged by size:
2867 1915 496 129
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4834
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2137 2857 3618 4646 1540
Size: 275 186 136 720 761 1028 2301
Fragments arranged by size:
2301 1028 761 720 275 186 136
BsiHKAI G_wGCw'C
Cuts at: 2237 3468 3553 4624 2237
Size: 1231 85 1071 3020
Fragments arranged by size:
3020 1231 1071 85
BsmI GAATG_Cn'
Cuts at: 2918 3011 2918
Size: 93 5314
BsmBI CGTCTCn'nnnn_
Cuts at: 2261 2261
Size: 5407
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2237 2244 2470 2696 2726 3468 3553
Size: 399 447 7 226 226 30 742 85
Cuts at: 3553 4624 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 447 399 226 226 85
30 7
BspEI T'CCGG_A
Cuts at: 2017 5070 2017
Size: 3053 2354
BspHI T'CATG_A
Cuts at: 3296 5026 3296
Size: 1730 3677
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2762 844
Size: 1918 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2080 3294 1394
Size: 429 257 1214 3507
Fragments arranged by size:
3507 1214 429 257
BsrDI GCAATG_nn'
Cuts at: 65 1248 3902 4076 65
Size: 1183 2654 174 1396
Fragments arranged by size:
2654 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2596 4051 4238 1700
Size: 110 786 1455 187 2869
Fragments arranged by size:
2869 1455 786 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5407
BssHII G'CGCG_C
Cuts at: 1925 2118 1925
Size: 193 5214
BssSI C'ACGA_G
Cuts at: 2140 3467 4479 2140
Size: 1327 1012 3068
Fragments arranged by size:
3068 1327 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5407
BstBI TT'CG_AA
Cuts at: 4211 4211
Size: 5407
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5407
BstXI CCAn_nnnn'nTGG
Cuts at: 2703 2703
Size: 5407
BstYI r'GATC_y
Cuts at: 1972 2737 3104 3489 3506 4958 5044 5056 5402
Size: 765 367 385 17 1452 86 12 346
Cuts at: 5402 1972
Size: 1977
Fragments arranged by size:
1977 1452 765 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2024 513
Size: 552 959 3896
Fragments arranged by size:
3896 959 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4668
Fragments arranged by size:
4668 683 56
BtsI GCAGTG_nn'
Cuts at: 2923 3717 3737 2923
Size: 794 20 4593
Fragments arranged by size:
4593 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5407
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5372
DraI TTT'AAA
Cuts at: 2711 2711
Size: 5407
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4414 809
Size: 507 3098 1802
Fragments arranged by size:
3098 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2134 2475 2854
Size: 54 1425 50 275 18 304 341 379
Cuts at: 2854 3743 5372 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 379 341 304 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2134 2854 1537
Size: 275 322 720 4090
Fragments arranged by size:
4090 720 322 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3337 1353
Size: 1984 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3994 4368 4514 387
Size: 1064 138 2405 374 146 1280
Fragments arranged by size:
2405 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2316 2710 3470 4067 4854 5122 1239
Size: 393 684 394 760 597 787 268 1524
Fragments arranged by size:
1524 787 760 684 597 394 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2096 2606 2096
Size: 510 4897
EcoO109I rG'GnC_Cy
Cuts at: 2241 2241
Size: 5407
EcoRI G'AATT_C
Cuts at: 2716 3094 2716
Size: 378 5029
FspI TGC'GCA
Cuts at: 3913 3913
Size: 5407
HaeII r_GCGC'y
Cuts at: 1678 2340 2383 4554 1678
Size: 662 43 2171 2531
Fragments arranged by size:
2531 2171 662 43
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4048 4080 4122 4154 1373
Size: 32 135 32 2476 32 42 32 2626
Fragments arranged by size:
2626 2476 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2751 2997 669
Size: 988 281 813 246 3079
Fragments arranged by size:
3079 988 813 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5407
HpaI GTT'AAC
Cuts at: 2997 2997
Size: 5407
KasI G'GCGC_C
Cuts at: 2336 2336
Size: 5407
KpnI G_GTAC'C
Cuts at: 2732 2732
Size: 5407
MfeI C'AATT_G
Cuts at: 3006 3006
Size: 5407
MluI A'CGCG_T
Cuts at: 5283 5283
Size: 5407
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2295 2400 4521 4705 1305
Size: 990 105 2121 184 2007
Fragments arranged by size:
2121 2007 990 184 105
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2477 10
Size: 54 1425 343 645 2940
Fragments arranged by size:
2940 1425 645 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2408 3365 3724 3883 518
Size: 648 1242 957 359 159 2042
Fragments arranged by size:
2042 1242 957 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2026 2125 2230 2688 2823 3500 4648 4893
Size: 420 99 105 458 135 677 1148 245
Cuts at: 4893 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 458 420 245 135 105
99
NaeI GCC'GGC
Cuts at: 2598 2598
Size: 5407
NarI GG'CG_CC
Cuts at: 2337 2337
Size: 5407
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4855
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5407
NgoMIV G'CCGG_C
Cuts at: 2596 2596
Size: 5407
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5407
NotI GC'GGCC_GC
Cuts at: 2134 2854 2134
Size: 720 4687
NspI r_CATG'y
Cuts at: 1132 1229 2765 4310 1132
Size: 97 1536 1545 2229
Fragments arranged by size:
2229 1545 1536 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5407
PciI A'CATG_T
Cuts at: 4306 4306
Size: 5407
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5407
PfoI T'CCnGG_A
Cuts at: 2097 2097
Size: 5407
PmeI GTTT'AAAC
Cuts at: 2711 2711
Size: 5407
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2414 2446 3476 3508 4989 5021 2414
Size: 32 1030 32 1481 32 2800
Fragments arranged by size:
2800 1481 1030 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5407
PsiI TTA'TAA
Cuts at: 2977 2977
Size: 5407
PspOMI G'GGCC_C
Cuts at: 2240 2466 2240
Size: 226 5181
PstI C_TGCA'G
Cuts at: 830 2759 830
Size: 1929 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5407
PvuII CAG'CTG
Cuts at: 2230 2688 2230
Size: 458 4949
SacII CC_GC'GG
Cuts at: 2027 2027
Size: 5407
SalI G'TCGA_C
Cuts at: 1936 2749 1936
Size: 813 4594
SbfI CC_TGCA'GG
Cuts at: 2759 2759
Size: 5407
ScaI AGT'ACT
Cuts at: 3655 5367 3655
Size: 1712 3695
SfcI C'TryA_G
Cuts at: 826 1045 2755 3890 4571 4762 826
Size: 219 1710 1135 681 191 1471
Fragments arranged by size:
1710 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2474 2474
Size: 5407
SfoI GGC'GCC
Cuts at: 2338 2338
Size: 5407
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5407
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5407
SmaI CCC'GGG
Cuts at: 1872 2032 2624 2734 1872
Size: 160 592 110 4545
Fragments arranged by size:
4545 592 160 110
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2486 3511 4412 4674 820
Size: 197 926 543 1025 901 262 1553
Fragments arranged by size:
1553 1025 926 901 543 262 197
SnaBI TAC'GTA
Cuts at: 493 3198 493
Size: 2705 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5407
SphI G_CATG'C
Cuts at: 1132 2765 1132
Size: 1633 3774
SspI AAT'ATT
Cuts at: 5 52 3331 5
Size: 47 3279 2081
Fragments arranged by size:
3279 2081 47
StuI AGG'CCT
Cuts at: 1517 2615 1517
Size: 1098 4309
StyI C'CwwG_G
Cuts at: 513 1065 2345 2846 513
Size: 552 1280 501 3074
Fragments arranged by size:
3074 1280 552 501
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2297 3434 3451 3604 3789 4234 325
Size: 1255 717 1137 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1137 717 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2673 3653 5365
Size: 274 80 33 51 1443 696 980 1712
Cuts at: 5365 96
Size: 138
Fragments arranged by size:
1712 1443 980 696 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5367 5399 5367
Size: 32 5375
Tth111I GACn'n_nGTC
Cuts at: 1316 2068 1316
Size: 752 4655
XbaI T'CTAG_A
Cuts at: 2743 2743
Size: 5407
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5407
XmaI C'CCGG_G
Cuts at: 1870 2030 2622 2732 1870
Size: 160 592 110 4545
Fragments arranged by size:
4545 592 160 110
XmnI GAAnn'nnTTC
Cuts at: 3536 3536
Size: 5407
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3215 276
Size: 53 83 186 936 1681 2468
Fragments arranged by size:
2468 1681 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I
Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NruI
NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI
SanDI SapI SexAI SrfI SwaI XcmI