Restriction map for
pFN21AB8899
(Circular) (Six-base) MAPSORT of: pFN21AB8899.seq Check: 4131 from: 1 to: 5404
pFN21AB8899 5404 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3214 278
Size: 53 83 186 936 1678 2468
Fragments arranged by size:
2468 1678 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2747 1937
Size: 810 4594
Acc65I G'GTAC_C
Cuts at: 2725 2725
Size: 5404
AclI AA'CG_TT
Cuts at: 1563 3531 3904 1563
Size: 1968 373 3063
Fragments arranged by size:
3063 1968 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3467 4851 5119 1632
Size: 1835 1384 268 1917
Fragments arranged by size:
1917 1835 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5207
AflIII A'CryG_T
Cuts at: 1203 4303 5280 1203
Size: 3100 977 1327
Fragments arranged by size:
3100 1327 977
AgeI A'CCGG_T
Cuts at: 4235 4235
Size: 5404
AhdI GACnn_n'nnGTC
Cuts at: 4133 4133
Size: 5404
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5404
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5404
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2507 2539 2507
Size: 32 5372
AlwNI CAG_nnn'CTG
Cuts at: 2506 3147 4719 2506
Size: 641 1572 3191
Fragments arranged by size:
3191 1572 641
ApaI G_GGCC'C
Cuts at: 2300 2300
Size: 5404
ApaLI G'TGCA_C
Cuts at: 3461 4617 3461
Size: 1156 4248
ApoI r'AATT_y
Cuts at: 1493 2713 2939 3091 5200 1493
Size: 1220 226 152 2109 1697
Fragments arranged by size:
2109 1697 1220 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3958 160
Size: 872 2926 1606
Fragments arranged by size:
2926 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5404
AvaI C'yCGr_G
Cuts at: 1870 1943 2036 2275 2300 2324 2729 1870
Size: 73 93 239 25 24 405 4545
Fragments arranged by size:
4545 405 239 93 73 25 24
BamHI G'GATC_C
Cuts at: 2734 3101 2734
Size: 367 5037
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2264 2384 2469 2550 2725
Size: 325 217 627 477 120 85 81 175
Cuts at: 2725 4180 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 477 325 217 175 120
85 81
BanII G_rGCy'C
Cuts at: 1391 2300 2408 2723 1391
Size: 909 108 315 4072
Fragments arranged by size:
4072 909 315 108
BbeI G_GCGC'C
Cuts at: 2268 2388 2473 2554 2268
Size: 120 85 81 5118
Fragments arranged by size:
5118 120 85 81
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4433
BbvCI CC'TCA_GC
Cuts at: 2108 2108
Size: 5404
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2548 2582 3595 3629 1903
Size: 34 611 34 1013 34 3678
Fragments arranged by size:
3678 1013 611 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3296 4506 3296
Size: 1210 4194
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5404
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4015 136
Size: 107 122 71 631 605 2343 1525
Fragments arranged by size:
2343 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2604 5399 2604
Size: 2795 2609
BlpI GC'TnA_GC
Cuts at: 2821 2821
Size: 5404
Bme1580I G_kGCm'C
Cuts at: 1790 2300 3465 4621 1790
Size: 510 1165 1156 2573
Fragments arranged by size:
2573 1165 1156 510
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5404
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3135 4093 442
Size: 416 53 83 399 24 1718 958 1753
Fragments arranged by size:
1753 1718 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5404
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2528 2534 4064 1239
Size: 1289 6 1530 2579
Fragments arranged by size:
2579 1530 1289 6
Bpu10I CC'TnA_GC
Cuts at: 2108 2108
Size: 5404
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3529 4394 4692 3529
Size: 865 298 4241
Fragments arranged by size:
4241 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4067 882
Size: 617 2568 2219
Fragments arranged by size:
2568 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3195 493
Size: 711 1991 2702
Fragments arranged by size:
2702 1991 711
BsaBI GATnn'nnATC
Cuts at: 2603 5398 2603
Size: 2795 2609
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2135 2213 2265 2385
Size: 53 83 186 936 602 78 52 120
Cuts at: 2385 2470 2551 3211 3593 4228 275
Size: 85 81 660 382 635 1451
Fragments arranged by size:
1451 936 660 635 602 382 186 120
85 83 81 78 53 52
BsaWI w'CCGG_w
Cuts at: 2391 3837 4235 4509 4656 5067 2391
Size: 1446 398 274 147 411 2728
Fragments arranged by size:
2728 1446 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2507 2537 1178
Size: 30 165 30 1104 30 4045
Fragments arranged by size:
4045 1104 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5404
BseYI C'CCAG_C
Cuts at: 2048 2669 4607 5103 2048
Size: 621 1938 496 2349
Fragments arranged by size:
2349 1938 621 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2297 1163
Size: 573 561 4270
Fragments arranged by size:
4270 573 561
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2392 2854 3615 4643 1540
Size: 275 186 391 462 761 1028 2301
Fragments arranged by size:
2301 1028 761 462 391 275 186
BsiHKAI G_wGCw'C
Cuts at: 3465 3550 4621 3465
Size: 85 1071 4248
Fragments arranged by size:
4248 1071 85
BsiWI C'GTAC_G
Cuts at: 2127 2127
Size: 5404
BsmI GAATG_Cn'
Cuts at: 2915 3008 2915
Size: 93 5311
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2300 2408 2723 3465 3550 4621 1391
Size: 399 510 108 315 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 510 399 315 108 85
BspEI T'CCGG_A
Cuts at: 5067 5067
Size: 5404
BspHI T'CATG_A
Cuts at: 3293 5023 3293
Size: 1730 3674
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2319 2759 844
Size: 1475 440 3489
Fragments arranged by size:
3489 1475 440
BsrBI CCG'CTC
Cuts at: 1394 1823 3291 1394
Size: 429 1468 3507
Fragments arranged by size:
3507 1468 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3899 4073 65
Size: 1183 2651 174 1396
Fragments arranged by size:
2651 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4048 4235 1700
Size: 110 2238 187 2869
Fragments arranged by size:
2869 2238 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5404
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5404
BssSI C'ACGA_G
Cuts at: 2233 2401 3464 4476 2233
Size: 168 1063 1012 3161
Fragments arranged by size:
3161 1063 1012 168
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5404
BstBI TT'CG_AA
Cuts at: 4208 4208
Size: 5404
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5404
BstYI r'GATC_y
Cuts at: 1972 2604 2734 3101 3486 3503 4955 5041 5053
Size: 632 130 367 385 17 1452 86 12
Cuts at: 5053 5399 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 632 385 367 346 130 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2464 513
Size: 552 1399 3453
Fragments arranged by size:
3453 1399 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4665
Fragments arranged by size:
4665 683 56
BtsI GCAGTG_nn'
Cuts at: 2287 2920 3714 3734 2287
Size: 633 794 20 3957
Fragments arranged by size:
3957 794 633 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5404
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5369
DraI TTT'AAA
Cuts at: 2708 2708
Size: 5404
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4411 809
Size: 507 3095 1802
Fragments arranged by size:
3095 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2053 2851 3740
Size: 54 1425 50 275 18 223 798 889
Cuts at: 3740 5369 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 798 275 223 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2851 1537
Size: 275 1039 4090
Fragments arranged by size:
4090 1039 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3334 1353
Size: 1981 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3991 4365 4511 387
Size: 1064 138 2402 374 146 1280
Fragments arranged by size:
2402 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2528 2534 3467 4064 4851 5119 1239
Size: 393 896 6 933 597 787 268 1524
Fragments arranged by size:
1524 933 896 787 597 393 268 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2087 2087
Size: 5404
EcoO109I rG'GnC_Cy
Cuts at: 2112 2229 2254 2296 2297 2112
Size: 117 25 42 1 5219
Fragments arranged by size:
5219 117 42 25 1
EcoRI G'AATT_C
Cuts at: 2713 3091 2713
Size: 378 5026
FspI TGC'GCA
Cuts at: 3910 3910
Size: 5404
HaeII r_GCGC'y
Cuts at: 1678 2268 2388 2473 2554 2596 4551 1678
Size: 590 120 85 81 42 1955 2531
Fragments arranged by size:
2531 1955 590 120 85 81 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4045 4077 4119 4151 1373
Size: 32 135 32 2473 32 42 32 2626
Fragments arranged by size:
2626 2473 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2748 2994 669
Size: 988 281 810 246 3079
Fragments arranged by size:
3079 988 810 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5404
HpaI GTT'AAC
Cuts at: 2994 2994
Size: 5404
KasI G'GCGC_C
Cuts at: 2264 2384 2469 2550 2264
Size: 120 85 81 5118
Fragments arranged by size:
5118 120 85 81
KpnI G_GTAC'C
Cuts at: 2729 2729
Size: 5404
MfeI C'AATT_G
Cuts at: 3003 3003
Size: 5404
MluI A'CGCG_T
Cuts at: 5280 5280
Size: 5404
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2211 4518 4702 1305
Size: 906 2307 184 2007
Fragments arranged by size:
2307 2007 906 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3582
Fragments arranged by size:
3582 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3362 3721 3880 518
Size: 648 2196 359 159 2042
Fragments arranged by size:
2196 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2052 2077 2355 2428 2673 2820 3497 4645
Size: 446 25 278 73 245 147 677 1148
Cuts at: 4645 4890 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 446 278 245 245 147
73 25
NarI GG'CG_CC
Cuts at: 2265 2385 2470 2551 2265
Size: 120 85 81 5118
Fragments arranged by size:
5118 120 85 81
NcoI C'CATG_G
Cuts at: 513 1065 2464 513
Size: 552 1399 3453
Fragments arranged by size:
3453 1399 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5404
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5404
NotI GC'GGCC_GC
Cuts at: 2851 2851
Size: 5404
NspI r_CATG'y
Cuts at: 1132 1229 2762 4307 1132
Size: 97 1533 1545 2229
Fragments arranged by size:
2229 1545 1533 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5404
PciI A'CATG_T
Cuts at: 4303 4303
Size: 5404
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2506 1108
Size: 1398 4006
PfoI T'CCnGG_A
Cuts at: 2145 2145
Size: 5404
PmeI GTTT'AAAC
Cuts at: 2708 2708
Size: 5404
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2507 2539 3473 3505 4986 5018 2507
Size: 32 934 32 1481 32 2893
Fragments arranged by size:
2893 1481 934 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2254 2254
Size: 5404
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5404
PsiI TTA'TAA
Cuts at: 2974 2974
Size: 5404
PspOMI G'GGCC_C
Cuts at: 2296 2296
Size: 5404
PstI C_TGCA'G
Cuts at: 830 2756 830
Size: 1926 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5404
PvuII CAG'CTG
Cuts at: 2077 2077
Size: 5404
SalI G'TCGA_C
Cuts at: 1936 2746 1936
Size: 810 4594
SbfI CC_TGCA'GG
Cuts at: 2756 2756
Size: 5404
ScaI AGT'ACT
Cuts at: 3652 5364 3652
Size: 1712 3692
SfcI C'TryA_G
Cuts at: 826 1045 2752 3887 4568 4759 826
Size: 219 1707 1135 681 191 1471
Fragments arranged by size:
1707 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2266 2386 2471 2552 2266
Size: 120 85 81 5118
Fragments arranged by size:
5118 120 85 81
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5404
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5404
SmaI CCC'GGG
Cuts at: 1872 2038 2302 2731 1872
Size: 166 264 429 4545
Fragments arranged by size:
4545 429 264 166
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3508 4409 4671 820
Size: 197 926 1565 901 262 1553
Fragments arranged by size:
1565 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3195 493
Size: 2702 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5404
SphI G_CATG'C
Cuts at: 1132 2762 1132
Size: 1630 3774
SspI AAT'ATT
Cuts at: 5 52 3328 5
Size: 47 3276 2081
Fragments arranged by size:
3276 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5404
StyI C'CwwG_G
Cuts at: 513 1065 2417 2464 2843 513
Size: 552 1352 47 379 3074
Fragments arranged by size:
3074 1352 552 379 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2182 3431 3448 3601 3786 4231 325
Size: 1255 602 1249 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1249 602 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2334 2397 3650
Size: 274 80 33 51 1443 357 63 1253
Cuts at: 3650 5362 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1253 357 274 138 80 63
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5364 5396 5364
Size: 32 5372
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5404
XbaI T'CTAG_A
Cuts at: 2740 2740
Size: 5404
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2194 2194
Size: 5404
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5404
XmaI C'CCGG_G
Cuts at: 1870 2036 2300 2729 1870
Size: 166 264 429 4545
Fragments arranged by size:
4545 429 264 166
XmnI GAAnn'nnTTC
Cuts at: 2522 3533 2522
Size: 1011 4393
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3212 276
Size: 53 83 186 936 1678 2468
Fragments arranged by size:
2468 1678 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BfrBI BplI BsmBI
BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SrfI SwaI