Restriction map for
pFN21AB8897
(Circular) (Six-base) MAPSORT of: pFN21AB8897.seq Check: 1542 from: 1 to: 5377
pFN21AB8897 5377 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3187 278
Size: 53 83 186 936 1651 2468
Fragments arranged by size:
2468 1651 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2385 2720 1937
Size: 448 335 4594
Fragments arranged by size:
4594 448 335
Acc65I G'GTAC_C
Cuts at: 2698 2698
Size: 5377
AclI AA'CG_TT
Cuts at: 1563 3504 3877 1563
Size: 1941 373 3063
Fragments arranged by size:
3063 1941 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3440 4824 5092 1632
Size: 1808 1384 268 1917
Fragments arranged by size:
1917 1808 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5180
AflIII A'CryG_T
Cuts at: 1203 2465 4276 5253 1203
Size: 1262 1811 977 1327
Fragments arranged by size:
1811 1327 1262 977
AgeI A'CCGG_T
Cuts at: 4208 4208
Size: 5377
AhdI GACnn_n'nnGTC
Cuts at: 4106 4106
Size: 5377
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5377
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5377
AlwNI CAG_nnn'CTG
Cuts at: 2479 3120 4692 2479
Size: 641 1572 3164
Fragments arranged by size:
3164 1572 641
ApaI G_GGCC'C
Cuts at: 2436 2436
Size: 5377
ApaLI G'TGCA_C
Cuts at: 3434 4590 3434
Size: 1156 4221
ApoI r'AATT_y
Cuts at: 1493 2407 2686 2912 3064 5173 1493
Size: 914 279 226 152 2109 1697
Fragments arranged by size:
2109 1697 914 279 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3931 160
Size: 872 2899 1606
Fragments arranged by size:
2899 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5377
AvaI C'yCGr_G
Cuts at: 1870 1943 2306 2702 1870
Size: 73 363 396 4545
Fragments arranged by size:
4545 396 363 73
BamHI G'GATC_C
Cuts at: 2707 3074 2707
Size: 367 5010
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2212 2233 2279 2698 4153
Size: 325 217 627 425 21 46 419 1455
Cuts at: 4153 618
Size: 1842
Fragments arranged by size:
1842 1455 627 425 419 325 217 46
21
BanII G_rGCy'C
Cuts at: 1391 2436 2696 1391
Size: 1045 260 4072
Fragments arranged by size:
4072 1045 260
BbeI G_GCGC'C
Cuts at: 2216 2237 2283 2216
Size: 21 46 5310
Fragments arranged by size:
5310 46 21
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4406
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2521 2555 3568 3602 1903
Size: 34 584 34 1013 34 3678
Fragments arranged by size:
3678 1013 584 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3269 4479 3269
Size: 1210 4167
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5377
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2134 2448 3988
Size: 107 122 71 631 605 462 314 1540
Cuts at: 3988 136
Size: 1525
Fragments arranged by size:
1540 1525 631 605 462 314 122 107
71
BglII A'GATC_T
Cuts at: 5372 5372
Size: 5377
BlpI GC'TnA_GC
Cuts at: 2627 2794 2627
Size: 167 5210
Bme1580I G_kGCm'C
Cuts at: 1790 2436 3438 4594 1790
Size: 646 1002 1156 2573
Fragments arranged by size:
2573 1156 1002 646
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5377
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3108 4066 442
Size: 416 53 83 399 24 1691 958 1753
Fragments arranged by size:
1753 1691 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5377
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2501 2507 4037 1239
Size: 1262 6 1530 2579
Fragments arranged by size:
2579 1530 1262 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3502 4367 4665 3502
Size: 865 298 4214
Fragments arranged by size:
4214 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4040 882
Size: 617 2541 2219
Fragments arranged by size:
2541 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3168 493
Size: 711 1964 2702
Fragments arranged by size:
2702 1964 711
BsaBI GATnn'nnATC
Cuts at: 5371 5371
Size: 5377
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2213 2234 2280 3184
Size: 53 83 186 936 680 21 46 904
Cuts at: 3184 3566 4201 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 904 680 635 382 186 83
53 46 21
BsaWI w'CCGG_w
Cuts at: 2380 3810 4208 4482 4629 5040 2380
Size: 1430 398 274 147 411 2717
Fragments arranged by size:
2717 1430 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2408 2438 2480 2510 1178
Size: 30 165 30 1005 30 42 30 4045
Fragments arranged by size:
4045 1005 165 42 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2666 2669 1186
Size: 1480 3 3894
Fragments arranged by size:
3894 1480 3
BseYI C'CCAG_C
Cuts at: 2435 4580 5076 2435
Size: 2145 496 2736
Fragments arranged by size:
2736 2145 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4804
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2206 2443 2827 3588 4616 1540
Size: 275 186 205 237 384 761 1028 2301
Fragments arranged by size:
2301 1028 761 384 275 237 205 186
BsiHKAI G_wGCw'C
Cuts at: 2375 3438 3523 4594 2375
Size: 1063 85 1071 3158
Fragments arranged by size:
3158 1071 1063 85
BsmI GAATG_Cn'
Cuts at: 2888 2981 2888
Size: 93 5284
BsmBI CGTCTCn'nnnn_
Cuts at: 2640 2640
Size: 5377
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2375 2436 2696 3438 3523 4594 1391
Size: 399 585 61 260 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 585 399 260 85 61
BspEI T'CCGG_A
Cuts at: 5040 5040
Size: 5377
BspHI T'CATG_A
Cuts at: 3266 4996 3266
Size: 1730 3647
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2732 844
Size: 1888 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2083 2210 3264 1394
Size: 429 260 127 1054 3507
Fragments arranged by size:
3507 1054 429 260 127
BsrDI GCAATG_nn'
Cuts at: 65 1248 3872 4046 65
Size: 1183 2624 174 1396
Fragments arranged by size:
2624 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2185 2230 2236 2442 4021 4208 1700
Size: 110 375 45 6 206 1579 187 2869
Fragments arranged by size:
2869 1579 375 206 187 110 45 6
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5377
BssHII G'CGCG_C
Cuts at: 1925 2328 1925
Size: 403 4974
BssSI C'ACGA_G
Cuts at: 3437 4449 3437
Size: 1012 4365
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5377
BstBI TT'CG_AA
Cuts at: 4181 4181
Size: 5377
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5377
BstYI r'GATC_y
Cuts at: 1972 2707 3074 3459 3476 4928 5014 5026 5372
Size: 735 367 385 17 1452 86 12 346
Cuts at: 5372 1972
Size: 1977
Fragments arranged by size:
1977 1452 735 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2312 2390 513
Size: 552 1247 78 3500
Fragments arranged by size:
3500 1247 552 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4638
Fragments arranged by size:
4638 683 56
BtsI GCAGTG_nn'
Cuts at: 2893 3687 3707 2893
Size: 794 20 4563
Fragments arranged by size:
4563 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5377
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5342
DraI TTT'AAA
Cuts at: 2681 2681
Size: 5377
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4384 809
Size: 507 3068 1802
Fragments arranged by size:
3068 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2046 2203 2440
Size: 54 1425 50 275 18 216 157 237
Cuts at: 2440 2824 3713 5342 8
Size: 384 889 1629 43
Fragments arranged by size:
1629 1425 889 384 275 237 216 157
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2203 2440 2824 1537
Size: 275 391 237 384 4090
Fragments arranged by size:
4090 391 384 275 237
EarI CTCTTCn'nnn_
Cuts at: 1353 2355 3307 1353
Size: 1002 952 3423
Fragments arranged by size:
3423 1002 952
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3964 4338 4484 387
Size: 1064 138 2375 374 146 1280
Fragments arranged by size:
2375 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2501 2507 3440 4037 4824 5092 1239
Size: 393 869 6 933 597 787 268 1524
Fragments arranged by size:
1524 933 869 787 597 393 268 6
EcoRI G'AATT_C
Cuts at: 2407 2686 3064 2407
Size: 279 378 4720
Fragments arranged by size:
4720 378 279
FspI TGC'GCA
Cuts at: 2269 3883 2269
Size: 1614 3763
HaeII r_GCGC'y
Cuts at: 1678 2216 2237 2283 4524 1678
Size: 538 21 46 2241 2531
Fragments arranged by size:
2531 2241 538 46 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2243 2275 4018 4050 4092
Size: 32 135 32 671 32 1743 32 42
Cuts at: 4092 4124 1373
Size: 32 2626
Fragments arranged by size:
2626 1743 671 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2721 2967 669
Size: 988 281 783 246 3079
Fragments arranged by size:
3079 988 783 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5377
HpaI GTT'AAC
Cuts at: 2967 2967
Size: 5377
KasI G'GCGC_C
Cuts at: 2212 2233 2279 2212
Size: 21 46 5310
Fragments arranged by size:
5310 46 21
KpnI G_GTAC'C
Cuts at: 2702 2702
Size: 5377
MfeI C'AATT_G
Cuts at: 2976 2976
Size: 5377
MluI A'CGCG_T
Cuts at: 5253 5253
Size: 5377
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2301 4491 4675 1305
Size: 996 2190 184 2007
Fragments arranged by size:
2190 2007 996 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2048 10
Size: 54 1425 343 216 3339
Fragments arranged by size:
3339 1425 343 216 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3335 3694 3853 518
Size: 648 2169 359 159 2042
Fragments arranged by size:
2169 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2052 2110 2314 2401 2439 2793 3470 4618
Size: 446 58 204 87 38 354 677 1148
Cuts at: 4618 4863 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 446 354 245 204 87
58 38
NaeI GCC'GGC
Cuts at: 2187 2232 2238 2444 2187
Size: 45 6 206 5120
Fragments arranged by size:
5120 206 45 6
NarI GG'CG_CC
Cuts at: 2213 2234 2280 2213
Size: 21 46 5310
Fragments arranged by size:
5310 46 21
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4825
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5377
NgoMIV G'CCGG_C
Cuts at: 2185 2230 2236 2442 2185
Size: 45 6 206 5120
Fragments arranged by size:
5120 206 45 6
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5377
NotI GC'GGCC_GC
Cuts at: 2824 2824
Size: 5377
NspI r_CATG'y
Cuts at: 1132 1229 2735 4280 1132
Size: 97 1506 1545 2229
Fragments arranged by size:
2229 1545 1506 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5377
PciI A'CATG_T
Cuts at: 4276 4276
Size: 5377
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2479 1108
Size: 1371 4006
PmeI GTTT'AAAC
Cuts at: 2681 2681
Size: 5377
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3446 3478 4959 4991 3446
Size: 32 1481 32 3832
Fragments arranged by size:
3832 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5377
PsiI TTA'TAA
Cuts at: 2947 2947
Size: 5377
PspOMI G'GGCC_C
Cuts at: 2432 2432
Size: 5377
PstI C_TGCA'G
Cuts at: 830 2729 830
Size: 1899 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5377
SacII CC_GC'GG
Cuts at: 2315 2315
Size: 5377
SalI G'TCGA_C
Cuts at: 1936 2719 1936
Size: 783 4594
SapI GCTCTTCn'nnn_
Cuts at: 2355 2355
Size: 5377
SbfI CC_TGCA'GG
Cuts at: 2729 2729
Size: 5377
ScaI AGT'ACT
Cuts at: 3625 5337 3625
Size: 1712 3665
SfcI C'TryA_G
Cuts at: 826 1045 2725 3860 4541 4732 826
Size: 219 1680 1135 681 191 1471
Fragments arranged by size:
1680 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2448 2448
Size: 5377
SfoI GGC'GCC
Cuts at: 2214 2235 2281 2214
Size: 21 46 5310
Fragments arranged by size:
5310 46 21
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5377
SgrAI Cr'CCGG_yG
Cuts at: 1700 2185 1700
Size: 485 4892
SmaI CCC'GGG
Cuts at: 1872 2704 1872
Size: 832 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3481 4382 4644 820
Size: 197 926 1538 901 262 1553
Fragments arranged by size:
1553 1538 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3168 493
Size: 2675 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5377
SphI G_CATG'C
Cuts at: 1132 2735 1132
Size: 1603 3774
SspI AAT'ATT
Cuts at: 5 52 3301 5
Size: 47 3249 2081
Fragments arranged by size:
3249 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5377
StyI C'CwwG_G
Cuts at: 513 1065 2816 513
Size: 552 1751 3074
Fragments arranged by size:
3074 1751 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3404 3421 3574 3759 4204 325
Size: 1255 1824 17 153 185 445 1498
Fragments arranged by size:
1824 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3623 5335 96
Size: 274 80 33 51 1443 1646 1712 138
Fragments arranged by size:
1712 1646 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5337 5369 5337
Size: 32 5345
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5377
XbaI T'CTAG_A
Cuts at: 2713 2713
Size: 5377
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5377
XmaI C'CCGG_G
Cuts at: 1870 2702 1870
Size: 832 4545
XmnI GAAnn'nnTTC
Cuts at: 3506 3506
Size: 5377
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3185 276
Size: 53 83 186 936 1651 2468
Fragments arranged by size:
2468 1651 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI SacII SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI NruI NsiI
PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII
SacI SanDI SexAI SrfI SwaI XcmI