Restriction map for
pFN21AB8891
(Circular) (Six-base) MAPSORT of: pFN21AB8891.seq Check: 1541 from: 1 to: 5338
pFN21AB8891 5338 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3148 278
Size: 53 83 186 936 1612 2468
Fragments arranged by size:
2468 1612 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2681 1937
Size: 744 4594
Acc65I G'GTAC_C
Cuts at: 2659 2659
Size: 5338
AclI AA'CG_TT
Cuts at: 1563 3465 3838 1563
Size: 1902 373 3063
Fragments arranged by size:
3063 1902 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3401 4785 5053 1632
Size: 1769 1384 268 1917
Fragments arranged by size:
1917 1769 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5141
AflIII A'CryG_T
Cuts at: 1203 4237 5214 1203
Size: 3034 977 1327
Fragments arranged by size:
3034 1327 977
AgeI A'CCGG_T
Cuts at: 4169 4169
Size: 5338
AhdI GACnn_n'nnGTC
Cuts at: 4067 4067
Size: 5338
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5338
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5338
AlwNI CAG_nnn'CTG
Cuts at: 3081 4653 3081
Size: 1572 3766
ApaLI G'TGCA_C
Cuts at: 3395 4551 3395
Size: 1156 4182
ApoI r'AATT_y
Cuts at: 1493 2308 2647 2873 3025 5134 1493
Size: 815 339 226 152 2109 1697
Fragments arranged by size:
2109 1697 815 339 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3892 160
Size: 872 2860 1606
Fragments arranged by size:
2860 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5338
AvaI C'yCGr_G
Cuts at: 1870 1943 2090 2663 1870
Size: 73 147 573 4545
Fragments arranged by size:
4545 573 147 73
BamHI G'GATC_C
Cuts at: 2668 3035 2668
Size: 367 4971
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2095 2659 4114 618
Size: 325 217 627 308 564 1455 1842
Fragments arranged by size:
1842 1455 627 564 325 308 217
BanII G_rGCy'C
Cuts at: 1391 2657 1391
Size: 1266 4072
BbeI G_GCGC'C
Cuts at: 2099 2099
Size: 5338
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2112 928
Size: 971 213 4154
Fragments arranged by size:
4154 971 213
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2530 2564 3529 3563 1903
Size: 34 593 34 965 34 3678
Fragments arranged by size:
3678 965 593 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3230 4440 3230
Size: 1210 4128
BclI T'GATC_A
Cuts at: 1548 2179 1548
Size: 631 4707
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2340 3949 136
Size: 107 122 71 631 605 668 1609 1525
Fragments arranged by size:
1609 1525 668 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5333 5333
Size: 5338
BlpI GC'TnA_GC
Cuts at: 2144 2755 2144
Size: 611 4727
Bme1580I G_kGCm'C
Cuts at: 1790 3399 4555 1790
Size: 1609 1156 2573
Fragments arranged by size:
2573 1609 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5338
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3069 4027 442
Size: 416 53 83 399 24 1652 958 1753
Fragments arranged by size:
1753 1652 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5338
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3998 1239
Size: 2759 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3463 4328 4626 3463
Size: 865 298 4175
Fragments arranged by size:
4175 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4001 882
Size: 617 2502 2219
Fragments arranged by size:
2502 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3129 493
Size: 711 1925 2702
Fragments arranged by size:
2702 1925 711
BsaBI GATnn'nnATC
Cuts at: 5332 5332
Size: 5338
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2096 3145 3527 4162
Size: 53 83 186 936 563 1049 382 635
Cuts at: 4162 275
Size: 1451
Fragments arranged by size:
1451 1049 936 635 563 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3771 4169 4443 4590 5001 3771
Size: 398 274 147 411 4108
Fragments arranged by size:
4108 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5113
Fragments arranged by size:
5113 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5338
BseYI C'CCAG_C
Cuts at: 4541 5037 4541
Size: 496 4842
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4765
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2122 2788 3549 4577 1540
Size: 275 186 121 666 761 1028 2301
Fragments arranged by size:
2301 1028 761 666 275 186 121
BsiHKAI G_wGCw'C
Cuts at: 3399 3484 4555 3399
Size: 85 1071 4182
Fragments arranged by size:
4182 1071 85
BsiWI C'GTAC_G
Cuts at: 2469 2469
Size: 5338
BsmI GAATG_Cn'
Cuts at: 2611 2849 2942 2611
Size: 238 93 5007
Fragments arranged by size:
5007 238 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2657 3399 3484 4555 1391
Size: 399 867 742 85 1071 2174
Fragments arranged by size:
2174 1071 867 742 399 85
BspEI T'CCGG_A
Cuts at: 5001 5001
Size: 5338
BspHI T'CATG_A
Cuts at: 3227 4957 3227
Size: 1730 3608
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2693 844
Size: 1849 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2112 2275 3225 1394
Size: 429 289 163 950 3507
Fragments arranged by size:
3507 950 429 289 163
BsrDI GCAATG_nn'
Cuts at: 65 1248 3833 4007 65
Size: 1183 2585 174 1396
Fragments arranged by size:
2585 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2075 3982 4169 1700
Size: 110 265 1907 187 2869
Fragments arranged by size:
2869 1907 265 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5338
BssHII G'CGCG_C
Cuts at: 1925 2130 1925
Size: 205 5133
BssSI C'ACGA_G
Cuts at: 3398 4410 3398
Size: 1012 4326
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5338
BstBI TT'CG_AA
Cuts at: 4142 4142
Size: 5338
BstEII G'GTnAC_C
Cuts at: 1184 2235 1184
Size: 1051 4287
BstYI r'GATC_y
Cuts at: 1972 2668 3035 3420 3437 4889 4975 4987 5333
Size: 696 367 385 17 1452 86 12 346
Cuts at: 5333 1972
Size: 1977
Fragments arranged by size:
1977 1452 696 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4786
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2466 2533 487
Size: 683 56 1240 67 3292
Fragments arranged by size:
3292 1240 683 67 56
BtsI GCAGTG_nn'
Cuts at: 2155 2262 2854 3648 3668 2155
Size: 107 592 794 20 3825
Fragments arranged by size:
3825 794 592 107 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5338
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5303
DraI TTT'AAA
Cuts at: 2379 2642 2379
Size: 263 5075
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4345 809
Size: 507 3029 1802
Fragments arranged by size:
3029 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2119 2785 3674
Size: 54 1425 50 275 18 289 666 889
Cuts at: 3674 5303 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 666 289 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2119 2785 1537
Size: 275 307 666 4090
Fragments arranged by size:
4090 666 307 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2263 3268 1353
Size: 910 1005 3423
Fragments arranged by size:
3423 1005 910
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2047 3925 4299 4445 387
Size: 1064 138 458 1878 374 146 1280
Fragments arranged by size:
1878 1280 1064 458 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3401 3998 4785 5053 1239
Size: 393 1769 597 787 268 1524
Fragments arranged by size:
1769 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2308 2647 3025 2308
Size: 339 378 4621
Fragments arranged by size:
4621 378 339
FspI TGC'GCA
Cuts at: 3844 3844
Size: 5338
HaeII r_GCGC'y
Cuts at: 1678 2099 4485 1678
Size: 421 2386 2531
Fragments arranged by size:
2531 2386 421
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2552 2584 3979 4011 4053
Size: 32 135 32 980 32 1395 32 42
Cuts at: 4053 4085 1373
Size: 32 2626
Fragments arranged by size:
2626 1395 980 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2682 2928 669
Size: 988 281 744 246 3079
Fragments arranged by size:
3079 988 744 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5338
HpaI GTT'AAC
Cuts at: 2928 2928
Size: 5338
KasI G'GCGC_C
Cuts at: 2095 2095
Size: 5338
KpnI G_GTAC'C
Cuts at: 2663 2663
Size: 5338
MfeI C'AATT_G
Cuts at: 2937 2937
Size: 5338
MluI A'CGCG_T
Cuts at: 5214 5214
Size: 5338
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2306 4452 4636 1305
Size: 1001 2146 184 2007
Fragments arranged by size:
2146 2007 1001 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3516
Fragments arranged by size:
3516 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2379 3296 3655 3814 518
Size: 648 1213 917 359 159 2042
Fragments arranged by size:
2042 1213 917 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2068 2294 2442 2459 2754 3431 4579 4824
Size: 462 226 148 17 295 677 1148 245
Cuts at: 4824 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 462 295 245 226 148
17
NarI GG'CG_CC
Cuts at: 2096 2096
Size: 5338
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4786
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5338
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5338
NotI GC'GGCC_GC
Cuts at: 2785 2785
Size: 5338
NspI r_CATG'y
Cuts at: 1132 1229 2538 2696 4241 1132
Size: 97 1309 158 1545 2229
Fragments arranged by size:
2229 1545 1309 158 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5338
PciI A'CATG_T
Cuts at: 4237 4237
Size: 5338
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5338
PmeI GTTT'AAAC
Cuts at: 2642 2642
Size: 5338
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3407 3439 4920 4952 3407
Size: 32 1481 32 3793
Fragments arranged by size:
3793 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2394 1613
Size: 781 4557
PsiI TTA'TAA
Cuts at: 2908 2908
Size: 5338
PstI C_TGCA'G
Cuts at: 830 2293 2441 2690 830
Size: 1463 148 249 3478
Fragments arranged by size:
3478 1463 249 148
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5338
PvuII CAG'CTG
Cuts at: 2294 2459 2294
Size: 165 5173
SalI G'TCGA_C
Cuts at: 1936 2680 1936
Size: 744 4594
SbfI CC_TGCA'GG
Cuts at: 2690 2690
Size: 5338
ScaI AGT'ACT
Cuts at: 3586 5298 3586
Size: 1712 3626
SexAI A'CCwGG_T
Cuts at: 2586 2586
Size: 5338
SfcI C'TryA_G
Cuts at: 826 1045 2289 2437 2686 3821 4502 4693 826
Size: 219 1244 148 249 1135 681 191 1471
Fragments arranged by size:
1471 1244 1135 681 249 219 191 148
SfoI GGC'GCC
Cuts at: 2097 2097
Size: 5338
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5338
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5338
SmaI CCC'GGG
Cuts at: 1872 2665 1872
Size: 793 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3442 4343 4605 820
Size: 197 926 1499 901 262 1553
Fragments arranged by size:
1553 1499 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3129 493
Size: 2636 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5338
SphI G_CATG'C
Cuts at: 1132 2696 1132
Size: 1564 3774
SspI AAT'ATT
Cuts at: 5 52 2481 3262 5
Size: 47 2429 781 2081
Fragments arranged by size:
2429 2081 781 47
StuI AGG'CCT
Cuts at: 1517 2086 1517
Size: 569 4769
StyI C'CwwG_G
Cuts at: 513 1065 2412 2777 513
Size: 552 1347 365 3074
Fragments arranged by size:
3074 1347 552 365
SwaI ATTT'AAAT
Cuts at: 2379 2379
Size: 5338
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3365 3382 3535 3720 4165 325
Size: 1255 1785 17 153 185 445 1498
Fragments arranged by size:
1785 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3584 5296 96
Size: 274 80 33 51 1443 1607 1712 138
Fragments arranged by size:
1712 1607 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2039 2071 5298 5330 2039
Size: 32 3227 32 2047
Fragments arranged by size:
3227 2047 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5338
XbaI T'CTAG_A
Cuts at: 2674 2674
Size: 5338
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5338
XmaI C'CCGG_G
Cuts at: 1870 2663 1870
Size: 793 4545
XmnI GAAnn'nnTTC
Cuts at: 2611 3467 2611
Size: 856 4482
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3146 276
Size: 53 83 186 936 1612 2468
Fragments arranged by size:
2468 1612 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SfiI
SrfI XcmI