Restriction map for
pFN21AB8889
(Circular) (Six-base) MAPSORT of: pFN21AB8889.seq Check: 2407 from: 1 to: 5317
pFN21AB8889 5317 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2406 3127 278
Size: 53 83 186 936 870 721 2468
Fragments arranged by size:
2468 936 870 721 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2660 1937
Size: 723 4594
Acc65I G'GTAC_C
Cuts at: 2638 2638
Size: 5317
AclI AA'CG_TT
Cuts at: 1563 3444 3817 1563
Size: 1881 373 3063
Fragments arranged by size:
3063 1881 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2497 3380 4764 5032 1632
Size: 865 883 1384 268 1917
Fragments arranged by size:
1917 1384 883 865 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5120
AflIII A'CryG_T
Cuts at: 1203 4216 5193 1203
Size: 3013 977 1327
Fragments arranged by size:
3013 1327 977
AgeI A'CCGG_T
Cuts at: 2184 4148 2184
Size: 1964 3353
AhdI GACnn_n'nnGTC
Cuts at: 4046 4046
Size: 5317
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5317
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5317
AlwNI CAG_nnn'CTG
Cuts at: 2425 3060 4632 2425
Size: 635 1572 3110
Fragments arranged by size:
3110 1572 635
ApaLI G'TGCA_C
Cuts at: 3374 4530 3374
Size: 1156 4161
ApoI r'AATT_y
Cuts at: 1493 2626 2852 3004 5113 1493
Size: 1133 226 152 2109 1697
Fragments arranged by size:
2109 1697 1133 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3871 160
Size: 872 2839 1606
Fragments arranged by size:
2839 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5317
AvaI C'yCGr_G
Cuts at: 1870 1943 2097 2114 2135 2216 2409 2642 1870
Size: 73 154 17 21 81 193 233 4545
Fragments arranged by size:
4545 233 193 154 81 73 21 17
BamHI G'GATC_C
Cuts at: 2647 3014 2647
Size: 367 4950
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2108 2122 2638 4093 618
Size: 325 217 627 321 14 516 1455 1842
Fragments arranged by size:
1842 1455 627 516 325 321 217 14
BanII G_rGCy'C
Cuts at: 1391 2444 2636 1391
Size: 1053 192 4072
Fragments arranged by size:
4072 1053 192
BbeI G_GCGC'C
Cuts at: 2126 2126
Size: 5317
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4346
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3508 3542 1903
Size: 34 1571 34 3678
Fragments arranged by size:
3678 1571 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3209 4419 3209
Size: 1210 4107
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5317
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3928 136
Size: 107 122 71 631 605 2256 1525
Fragments arranged by size:
2256 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5312 5312
Size: 5317
BlpI GC'TnA_GC
Cuts at: 2438 2734 2438
Size: 296 5021
Bme1580I G_kGCm'C
Cuts at: 1790 3378 4534 1790
Size: 1588 1156 2573
Fragments arranged by size:
2573 1588 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5317
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3048 4006 442
Size: 416 53 83 399 24 1631 958 1753
Fragments arranged by size:
1753 1631 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5317
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2348 3977 1239
Size: 1109 1629 2579
Fragments arranged by size:
2579 1629 1109
Bpu10I CC'TnA_GC
Cuts at: 2481 2481
Size: 5317
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3442 4307 4605 3442
Size: 865 298 4154
Fragments arranged by size:
4154 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3980 882
Size: 617 2481 2219
Fragments arranged by size:
2481 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3108 493
Size: 711 1904 2702
Fragments arranged by size:
2702 1904 711
BsaBI GATnn'nnATC
Cuts at: 5311 5311
Size: 5317
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2123 2403 3124 3506
Size: 53 83 186 936 590 280 721 382
Cuts at: 3506 4141 275
Size: 635 1451
Fragments arranged by size:
1451 936 721 635 590 382 280 186
83 53
BsaWI w'CCGG_w
Cuts at: 2184 3750 4148 4422 4569 4980 2184
Size: 1566 398 274 147 411 2521
Fragments arranged by size:
2521 1566 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5092
Fragments arranged by size:
5092 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5317
BseYI C'CCAG_C
Cuts at: 2490 4520 5016 2490
Size: 2030 496 2791
Fragments arranged by size:
2791 2030 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4744
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2163 2767 3528 4556 1540
Size: 275 186 162 604 761 1028 2301
Fragments arranged by size:
2301 1028 761 604 275 186 162
BsiHKAI G_wGCw'C
Cuts at: 2262 2444 3378 3463 4534 2262
Size: 182 934 85 1071 3045
Fragments arranged by size:
3045 1071 934 182 85
BsmI GAATG_Cn'
Cuts at: 2828 2921 2828
Size: 93 5224
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2262 2444 2636 3378 3463 4534 1391
Size: 399 472 182 192 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 472 399 192 182 85
BspEI T'CCGG_A
Cuts at: 4980 4980
Size: 5317
BspHI T'CATG_A
Cuts at: 3206 4936 3206
Size: 1730 3587
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2672 844
Size: 1828 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2065 3204 1394
Size: 429 242 1139 3507
Fragments arranged by size:
3507 1139 429 242
BsrDI GCAATG_nn'
Cuts at: 65 1248 3812 3986 65
Size: 1183 2564 174 1396
Fragments arranged by size:
2564 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2119 2184 3961 4148 1700
Size: 110 309 65 1777 187 2869
Fragments arranged by size:
2869 1777 309 187 110 65
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5317
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5317
BssSI C'ACGA_G
Cuts at: 2303 3377 4389 2303
Size: 1074 1012 3231
Fragments arranged by size:
3231 1074 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5317
BstBI TT'CG_AA
Cuts at: 4121 4121
Size: 5317
BstEII G'GTnAC_C
Cuts at: 1184 2344 1184
Size: 1160 4157
BstYI r'GATC_y
Cuts at: 1972 2647 3014 3399 3416 4868 4954 4966 5312
Size: 675 367 385 17 1452 86 12 346
Cuts at: 5312 1972
Size: 1977
Fragments arranged by size:
1977 1452 675 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2034 2157 2378 513
Size: 552 939 30 123 221 3452
Fragments arranged by size:
3452 939 552 221 123 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4578
Fragments arranged by size:
4578 683 56
BtsI GCAGTG_nn'
Cuts at: 2833 3627 3647 2833
Size: 794 20 4503
Fragments arranged by size:
4503 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5317
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5282
DraI TTT'AAA
Cuts at: 2621 2621
Size: 5317
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4324 809
Size: 507 3008 1802
Fragments arranged by size:
3008 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2037 2073 2160
Size: 54 1425 50 275 18 207 36 87
Cuts at: 2160 2764 3653 5282 8
Size: 604 889 1629 43
Fragments arranged by size:
1629 1425 889 604 275 207 87 54
50 43 36 18
EagI C'GGCC_G
Cuts at: 1537 1812 2160 2764 1537
Size: 275 348 604 4090
Fragments arranged by size:
4090 604 348 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3247 1353
Size: 1894 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2188 2264 3904 4278 4424 387
Size: 1064 138 599 76 1640 374 146 1280
Fragments arranged by size:
1640 1280 1064 599 374 146 138 76
EcoICRI GAG'CTC
Cuts at: 2442 2442
Size: 5317
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2348 2497 3380 3977 4764 5032 1239
Size: 393 716 149 883 597 787 268 1524
Fragments arranged by size:
1524 883 787 716 597 393 268 149
EcoO109I rG'GnC_Cy
Cuts at: 2517 2517
Size: 5317
EcoRI G'AATT_C
Cuts at: 2626 3004 2626
Size: 378 4939
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2386 2386
Size: 5317
FspI TGC'GCA
Cuts at: 3823 3823
Size: 5317
HaeII r_GCGC'y
Cuts at: 1678 2126 2243 2425 4464 1678
Size: 448 117 182 2039 2531
Fragments arranged by size:
2531 2039 448 182 117
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3958 3990 4032 4064 1373
Size: 32 135 32 2386 32 42 32 2626
Fragments arranged by size:
2626 2386 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2011 2580 2661 2907 669
Size: 988 281 73 569 81 246 3079
Fragments arranged by size:
3079 988 569 281 246 81 73
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5317
HpaI GTT'AAC
Cuts at: 2580 2907 2580
Size: 327 4990
KasI G'GCGC_C
Cuts at: 2122 2122
Size: 5317
KpnI G_GTAC'C
Cuts at: 2642 2642
Size: 5317
MfeI C'AATT_G
Cuts at: 2916 2916
Size: 5317
MluI A'CGCG_T
Cuts at: 5193 5193
Size: 5317
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2289 4431 4615 1305
Size: 984 2142 184 2007
Fragments arranged by size:
2142 2007 984 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3495
Fragments arranged by size:
3495 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2033 3275 3634 3793 518
Size: 648 867 1242 359 159 2042
Fragments arranged by size:
2042 1242 867 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2047 2159 2341 2380 2551 2733 3410 4558
Size: 441 112 182 39 171 182 677 1148
Cuts at: 4558 4803 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 441 245 182 182 171
112 39
NaeI GCC'GGC
Cuts at: 2121 2121
Size: 5317
NarI GG'CG_CC
Cuts at: 2123 2123
Size: 5317
NcoI C'CATG_G
Cuts at: 513 1065 2004 2034 513
Size: 552 939 30 3796
Fragments arranged by size:
3796 939 552 30
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5317
NgoMIV G'CCGG_C
Cuts at: 2119 2119
Size: 5317
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5317
NotI GC'GGCC_GC
Cuts at: 2160 2764 2160
Size: 604 4713
NspI r_CATG'y
Cuts at: 1132 1229 2675 4220 1132
Size: 97 1446 1545 2229
Fragments arranged by size:
2229 1545 1446 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5317
PciI A'CATG_T
Cuts at: 4216 4216
Size: 5317
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2425 1108
Size: 1317 4000
PmeI GTTT'AAAC
Cuts at: 2621 2621
Size: 5317
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3386 3418 4899 4931 3386
Size: 32 1481 32 3772
Fragments arranged by size:
3772 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2517 2517
Size: 5317
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5317
PsiI TTA'TAA
Cuts at: 2887 2887
Size: 5317
PstI C_TGCA'G
Cuts at: 830 2478 2669 830
Size: 1648 191 3478
Fragments arranged by size:
3478 1648 191
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5317
PvuII CAG'CTG
Cuts at: 2551 2551
Size: 5317
SacI G_AGCT'C
Cuts at: 2444 2444
Size: 5317
SacII CC_GC'GG
Cuts at: 2160 2381 2160
Size: 221 5096
SalI G'TCGA_C
Cuts at: 1936 2659 1936
Size: 723 4594
SbfI CC_TGCA'GG
Cuts at: 2669 2669
Size: 5317
ScaI AGT'ACT
Cuts at: 3565 5277 3565
Size: 1712 3605
SfcI C'TryA_G
Cuts at: 826 1045 2474 2665 3800 4481 4672 826
Size: 219 1429 191 1135 681 191 1471
Fragments arranged by size:
1471 1429 1135 681 219 191 191
SfoI GGC'GCC
Cuts at: 2124 2124
Size: 5317
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5317
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5317
SmaI CCC'GGG
Cuts at: 1872 2099 2218 2411 2644 1872
Size: 227 119 193 233 4545
Fragments arranged by size:
4545 233 227 193 119
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3421 4322 4584 820
Size: 197 926 1478 901 262 1553
Fragments arranged by size:
1553 1478 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3108 493
Size: 2615 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5317
SphI G_CATG'C
Cuts at: 1132 2675 1132
Size: 1543 3774
SspI AAT'ATT
Cuts at: 5 52 3241 5
Size: 47 3189 2081
Fragments arranged by size:
3189 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5317
StyI C'CwwG_G
Cuts at: 513 1065 2004 2034 2756 513
Size: 552 939 30 722 3074
Fragments arranged by size:
3074 939 722 552 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3344 3361 3514 3699 4144 325
Size: 1255 1764 17 153 185 445 1498
Fragments arranged by size:
1764 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3563 5275 96
Size: 274 80 33 51 1443 1586 1712 138
Fragments arranged by size:
1712 1586 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5277 5309 5277
Size: 32 5285
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5317
XbaI T'CTAG_A
Cuts at: 2653 2653
Size: 5317
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2535 2535
Size: 5317
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5317
XmaI C'CCGG_G
Cuts at: 1870 2097 2216 2409 2642 1870
Size: 227 119 193 233 4545
Fragments arranged by size:
4545 233 227 193 119
XmnI GAAnn'nnTTC
Cuts at: 2225 3446 2225
Size: 1221 4096
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2404 3125 276
Size: 53 83 186 936 870 721 2468
Fragments arranged by size:
2468 936 870 721 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoNI EcoRV FseI FspAI NruI NsiI PacI PfoI
PmlI PspOMI PspXI PsrI RsrII SanDI SapI SexAI
SfiI SrfI SwaI