Restriction map for
pFN21AB8887
(Circular) (Six-base) MAPSORT of: pFN21AB8887.seq Check: 9586 from: 1 to: 5299
pFN21AB8887 5299 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2377 2377
Size: 5299
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2457 3109 278
Size: 53 83 186 936 921 652 2468
Fragments arranged by size:
2468 936 921 652 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2642 1937
Size: 705 4594
Acc65I G'GTAC_C
Cuts at: 2620 2620
Size: 5299
AclI AA'CG_TT
Cuts at: 1563 3426 3799 1563
Size: 1863 373 3063
Fragments arranged by size:
3063 1863 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2226 3362 4746 5014 1632
Size: 594 1136 1384 268 1917
Fragments arranged by size:
1917 1384 1136 594 268
AflII C'TTAA_G
Cuts at: 820 1017 2044 820
Size: 197 1027 4075
Fragments arranged by size:
4075 1027 197
AflIII A'CryG_T
Cuts at: 1203 4198 5175 1203
Size: 2995 977 1327
Fragments arranged by size:
2995 1327 977
AgeI A'CCGG_T
Cuts at: 4130 4130
Size: 5299
AhdI GACnn_n'nnGTC
Cuts at: 4028 4028
Size: 5299
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5299
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5299
AlwNI CAG_nnn'CTG
Cuts at: 2296 3042 4614 2296
Size: 746 1572 2981
Fragments arranged by size:
2981 1572 746
ApaI G_GGCC'C
Cuts at: 2237 2237
Size: 5299
ApaLI G'TGCA_C
Cuts at: 3356 4512 3356
Size: 1156 4143
ApoI r'AATT_y
Cuts at: 1493 2608 2834 2986 5095 1493
Size: 1115 226 152 2109 1697
Fragments arranged by size:
2109 1697 1115 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3853 160
Size: 872 2821 1606
Fragments arranged by size:
2821 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5299
AvaI C'yCGr_G
Cuts at: 1870 1943 2011 2082 2507 2624 1870
Size: 73 68 71 425 117 4545
Fragments arranged by size:
4545 425 117 73 71 68
BamHI G'GATC_C
Cuts at: 2629 2996 2629
Size: 367 4932
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2335 2620 4075 618
Size: 325 217 627 548 285 1455 1842
Fragments arranged by size:
1842 1455 627 548 325 285 217
BanII G_rGCy'C
Cuts at: 1391 2025 2237 2618 1391
Size: 634 212 381 4072
Fragments arranged by size:
4072 634 381 212
BbeI G_GCGC'C
Cuts at: 2339 2339
Size: 5299
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2311 928
Size: 971 412 3916
Fragments arranged by size:
3916 971 412
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3490 3524 1903
Size: 34 1553 34 3678
Fragments arranged by size:
3678 1553 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3191 4401 3191
Size: 1210 4089
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5299
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2547 3910 136
Size: 107 122 71 631 605 875 1363 1525
Fragments arranged by size:
1525 1363 875 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5294 5294
Size: 5299
BlpI GC'TnA_GC
Cuts at: 2497 2716 2497
Size: 219 5080
Bme1580I G_kGCm'C
Cuts at: 1790 2237 3360 4516 1790
Size: 447 1123 1156 2573
Fragments arranged by size:
2573 1156 1123 447
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5299
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3030 3988 442
Size: 416 53 83 399 24 1613 958 1753
Fragments arranged by size:
1753 1613 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2360 1056
Size: 1304 3995
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2438 2438
Size: 5299
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2168 2318 2324 2502 3959 1239
Size: 929 150 6 178 1457 2579
Fragments arranged by size:
2579 1457 929 178 150 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2408 3424 4289 4587 2408
Size: 1016 865 298 3120
Fragments arranged by size:
3120 1016 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3962 882
Size: 617 2463 2219
Fragments arranged by size:
2463 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3090 493
Size: 711 1886 2702
Fragments arranged by size:
2702 1886 711
BsaBI GATnn'nnATC
Cuts at: 5293 5293
Size: 5299
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2336 2454 3106 3488
Size: 53 83 186 936 803 118 652 382
Cuts at: 3488 4123 275
Size: 635 1451
Fragments arranged by size:
1451 936 803 652 635 382 186 118
83 53
BsaWI w'CCGG_w
Cuts at: 2221 3732 4130 4404 4551 4962 2221
Size: 1511 398 274 147 411 2558
Fragments arranged by size:
2558 1511 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5074
Fragments arranged by size:
5074 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2165 2453 2594 1186
Size: 979 288 141 3891
Fragments arranged by size:
3891 979 288 141
BseYI C'CCAG_C
Cuts at: 2230 4502 4998 2230
Size: 2272 496 2531
Fragments arranged by size:
2531 2272 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2512 1163
Size: 573 776 3950
Fragments arranged by size:
3950 776 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2251 2749 3510 4538 1540
Size: 275 186 250 498 761 1028 2301
Fragments arranged by size:
2301 1028 761 498 275 250 186
BsiHKAI G_wGCw'C
Cuts at: 2089 3360 3445 4516 2089
Size: 1271 85 1071 2872
Fragments arranged by size:
2872 1271 1071 85
BsmI GAATG_Cn'
Cuts at: 2810 2903 2810
Size: 93 5206
BsmBI CGTCTCn'nnnn_
Cuts at: 2343 2343
Size: 5299
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2025 2089 2237 2618 3360 3445 4516
Size: 399 235 64 148 381 742 85 1071
Cuts at: 4516 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 381 235 148 85
64
BspEI T'CCGG_A
Cuts at: 2221 4962 2221
Size: 2741 2558
BspHI T'CATG_A
Cuts at: 3188 4918 3188
Size: 1730 3569
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2207 2377 2654 844
Size: 1363 170 277 3489
Fragments arranged by size:
3489 1363 277 170
BsrBI CCG'CTC
Cuts at: 1394 1823 2308 3186 1394
Size: 429 485 878 3507
Fragments arranged by size:
3507 878 485 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3794 3968 65
Size: 1183 2546 174 1396
Fragments arranged by size:
2546 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2409 2546 3943 4130 1700
Size: 110 599 137 1397 187 2869
Fragments arranged by size:
2869 1397 599 187 137 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5299
BssHII G'CGCG_C
Cuts at: 1925 2142 2186 2188 2446 1925
Size: 217 44 2 258 4778
Fragments arranged by size:
4778 258 217 44 2
BssSI C'ACGA_G
Cuts at: 3359 4371 3359
Size: 1012 4287
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2093 2293 794
Size: 1299 200 3800
Fragments arranged by size:
3800 1299 200
BstBI TT'CG_AA
Cuts at: 4103 4103
Size: 5299
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5299
BstYI r'GATC_y
Cuts at: 1972 2521 2629 2996 3381 3398 4850 4936 4948
Size: 549 108 367 385 17 1452 86 12
Cuts at: 4948 5294 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 549 385 367 346 108 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2173 513
Size: 552 1108 3639
Fragments arranged by size:
3639 1108 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2035 2445 487
Size: 683 56 809 410 3341
Fragments arranged by size:
3341 809 683 410 56
BtsI GCAGTG_nn'
Cuts at: 2271 2501 2815 3609 3629 2271
Size: 230 314 794 20 3941
Fragments arranged by size:
3941 794 314 230 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5299
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5264
DraI TTT'AAA
Cuts at: 2603 2603
Size: 5299
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4306 809
Size: 507 2990 1802
Fragments arranged by size:
2990 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2248 2376 2746
Size: 54 1425 50 275 18 418 128 370
Cuts at: 2746 3635 5264 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 418 370 275 128 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2248 2746 1537
Size: 275 436 498 4090
Fragments arranged by size:
4090 498 436 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3229 1353
Size: 1876 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3886 4260 4406 387
Size: 1064 138 2297 374 146 1280
Fragments arranged by size:
2297 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2168 2226 2318 2324 2502 3362 3959
Size: 393 536 58 92 6 178 860 597
Cuts at: 3959 4746 5014 1239
Size: 787 268 1524
Fragments arranged by size:
1524 860 787 597 536 393 268 178
92 58 6
EcoO109I rG'GnC_Cy
Cuts at: 2234 2234
Size: 5299
EcoRI G'AATT_C
Cuts at: 2608 2986 2608
Size: 378 4921
FspI TGC'GCA
Cuts at: 3805 3805
Size: 5299
HaeII r_GCGC'y
Cuts at: 1678 2314 2339 2517 4446 1678
Size: 636 25 178 1929 2531
Fragments arranged by size:
2531 1929 636 178 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2444 2476 3940 3972 4014
Size: 32 135 32 872 32 1464 32 42
Cuts at: 4014 4046 1373
Size: 32 2626
Fragments arranged by size:
2626 1464 872 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2643 2889 669
Size: 988 281 705 246 3079
Fragments arranged by size:
3079 988 705 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5299
HpaI GTT'AAC
Cuts at: 2889 2889
Size: 5299
KasI G'GCGC_C
Cuts at: 2335 2335
Size: 5299
KpnI G_GTAC'C
Cuts at: 2624 2624
Size: 5299
MfeI C'AATT_G
Cuts at: 2898 2898
Size: 5299
MluI A'CGCG_T
Cuts at: 5175 5175
Size: 5299
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2150 4413 4597 1305
Size: 845 2263 184 2007
Fragments arranged by size:
2263 2007 845 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2378 10
Size: 54 1425 343 546 2931
Fragments arranged by size:
2931 1425 546 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3257 3616 3775 518
Size: 648 2091 359 159 2042
Fragments arranged by size:
2091 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2137 2175 2715 3392 4540 4785 1606
Size: 531 38 540 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 540 531 245 38
NaeI GCC'GGC
Cuts at: 2548 2548
Size: 5299
NarI GG'CG_CC
Cuts at: 2336 2336
Size: 5299
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4747
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5299
NgoMIV G'CCGG_C
Cuts at: 2546 2546
Size: 5299
NheI G'CTAG_C
Cuts at: 1052 2356 1052
Size: 1304 3995
NotI GC'GGCC_GC
Cuts at: 2746 2746
Size: 5299
NspI r_CATG'y
Cuts at: 1132 1229 2657 4202 1132
Size: 97 1428 1545 2229
Fragments arranged by size:
2229 1545 1428 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5299
PciI A'CATG_T
Cuts at: 4198 4198
Size: 5299
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5299
PmeI GTTT'AAAC
Cuts at: 2603 2603
Size: 5299
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3368 3400 4881 4913 3368
Size: 32 1481 32 3754
Fragments arranged by size:
3754 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5299
PsiI TTA'TAA
Cuts at: 2869 2869
Size: 5299
PspOMI G'GGCC_C
Cuts at: 2233 2233
Size: 5299
PstI C_TGCA'G
Cuts at: 830 2199 2651 830
Size: 1369 452 3478
Fragments arranged by size:
3478 1369 452
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5299
SacII CC_GC'GG
Cuts at: 2176 2176
Size: 5299
SalI G'TCGA_C
Cuts at: 1936 2641 1936
Size: 705 4594
SbfI CC_TGCA'GG
Cuts at: 2651 2651
Size: 5299
ScaI AGT'ACT
Cuts at: 3547 5259 3547
Size: 1712 3587
SfcI C'TryA_G
Cuts at: 826 1045 2195 2647 3782 4463 4654 826
Size: 219 1150 452 1135 681 191 1471
Fragments arranged by size:
1471 1150 1135 681 452 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2547 2547
Size: 5299
SfoI GGC'GCC
Cuts at: 2337 2337
Size: 5299
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5299
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5299
SmaI CCC'GGG
Cuts at: 1872 2626 1872
Size: 754 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2044 2423 3403 4304 4566 820
Size: 197 926 101 379 980 901 262 1553
Fragments arranged by size:
1553 980 926 901 379 262 197 101
SnaBI TAC'GTA
Cuts at: 493 3090 493
Size: 2597 2702
SpeI A'CTAG_T
Cuts at: 152 2159 152
Size: 2007 3292
SphI G_CATG'C
Cuts at: 1132 2657 1132
Size: 1525 3774
SspI AAT'ATT
Cuts at: 5 52 3223 5
Size: 47 3171 2081
Fragments arranged by size:
3171 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5299
StyI C'CwwG_G
Cuts at: 513 1065 2738 513
Size: 552 1673 3074
Fragments arranged by size:
3074 1673 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3326 3343 3496 3681 4126 325
Size: 1255 1746 17 153 185 445 1498
Fragments arranged by size:
1746 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3545 5257 96
Size: 274 80 33 51 1443 1568 1712 138
Fragments arranged by size:
1712 1568 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2474 2506 5259 5291 2474
Size: 32 2753 32 2482
Fragments arranged by size:
2753 2482 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5299
XbaI T'CTAG_A
Cuts at: 2635 2635
Size: 5299
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2386 2386
Size: 5299
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5299
XmaI C'CCGG_G
Cuts at: 1870 2624 1870
Size: 754 4545
XmnI GAAnn'nnTTC
Cuts at: 3428 3428
Size: 5299
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2455 3107 276
Size: 53 83 186 936 921 652 2468
Fragments arranged by size:
2468 936 921 652 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI SacII SalI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI NruI NsiI PacI PfoI PmlI
PpuMI PspXI PsrI PvuII RsrII SacI SanDI SapI
SexAI SrfI SwaI