Restriction map for
pFN21AB8879
(Circular) (Six-base) MAPSORT of: pFN21AB8879.seq Check: 2489 from: 1 to: 5224
pFN21AB8879 5224 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3034 278
Size: 53 83 186 936 1498 2468
Fragments arranged by size:
2468 1498 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2567 1937
Size: 630 4594
Acc65I G'GTAC_C
Cuts at: 2545 2545
Size: 5224
AclI AA'CG_TT
Cuts at: 1563 3351 3724 1563
Size: 1788 373 3063
Fragments arranged by size:
3063 1788 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3287 4671 4939 1632
Size: 1655 1384 268 1917
Fragments arranged by size:
1917 1655 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5027
AflIII A'CryG_T
Cuts at: 1203 4123 5100 1203
Size: 2920 977 1327
Fragments arranged by size:
2920 1327 977
AgeI A'CCGG_T
Cuts at: 4055 4055
Size: 5224
AhdI GACnn_n'nnGTC
Cuts at: 3953 3953
Size: 5224
AleI CACnn'nnGTG
Cuts at: 1166 2451 1166
Size: 1285 3939
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2440 1184
Size: 1256 3968
AlwNI CAG_nnn'CTG
Cuts at: 2096 2967 4539 2096
Size: 871 1572 2781
Fragments arranged by size:
2781 1572 871
ApaLI G'TGCA_C
Cuts at: 2415 3281 4437 2415
Size: 866 1156 3202
Fragments arranged by size:
3202 1156 866
ApoI r'AATT_y
Cuts at: 1493 2533 2759 2911 5020 1493
Size: 1040 226 152 2109 1697
Fragments arranged by size:
2109 1697 1040 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3778 160
Size: 872 2746 1606
Fragments arranged by size:
2746 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5224
AvaI C'yCGr_G
Cuts at: 1870 1943 2341 2428 2549 1870
Size: 73 398 87 121 4545
Fragments arranged by size:
4545 398 121 87 73
BamHI G'GATC_C
Cuts at: 2554 2921 2554
Size: 367 4857
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2074 2242 2545 4000 618
Size: 325 217 627 287 168 303 1455 1842
Fragments arranged by size:
1842 1455 627 325 303 287 217 168
BanII G_rGCy'C
Cuts at: 1391 2106 2380 2543 1391
Size: 715 274 163 4072
Fragments arranged by size:
4072 715 274 163
BbeI G_GCGC'C
Cuts at: 2246 2246
Size: 5224
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2320 928
Size: 971 421 3832
Fragments arranged by size:
3832 971 421
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3415 3449 1903
Size: 34 1478 34 3678
Fragments arranged by size:
3678 1478 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3116 4326 3116
Size: 1210 4014
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5224
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2115 2255 2346
Size: 107 122 71 631 605 443 140 91
Cuts at: 2346 3835 136
Size: 1489 1525
Fragments arranged by size:
1525 1489 631 605 443 140 122 107
91 71
BglII A'GATC_T
Cuts at: 5219 5219
Size: 5224
BlpI GC'TnA_GC
Cuts at: 2641 2641
Size: 5224
Bme1580I G_kGCm'C
Cuts at: 1790 2419 3285 4441 1790
Size: 629 866 1156 2573
Fragments arranged by size:
2573 1156 866 629
BmgBI CAC'GTC
Cuts at: 1318 2486 1318
Size: 1168 4056
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2025 2955 3913
Size: 416 53 83 399 24 608 930 958
Cuts at: 3913 442
Size: 1753
Fragments arranged by size:
1753 958 930 608 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5224
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2133 3884 1239
Size: 894 1751 2579
Fragments arranged by size:
2579 1751 894
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3349 4214 4512 3349
Size: 865 298 4061
Fragments arranged by size:
4061 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3887 882
Size: 617 2388 2219
Fragments arranged by size:
2388 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3015 493
Size: 711 1811 2702
Fragments arranged by size:
2702 1811 711
BsaBI GATnn'nnATC
Cuts at: 5218 5218
Size: 5224
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2243 3031 3413 4048
Size: 53 83 186 936 710 788 382 635
Cuts at: 4048 275
Size: 1451
Fragments arranged by size:
1451 936 788 710 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2154 3657 4055 4329 4476 4887 2154
Size: 1503 398 274 147 411 2491
Fragments arranged by size:
2491 1503 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2272 2302 1178
Size: 30 165 30 869 30 4100
Fragments arranged by size:
4100 869 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2293 1186
Size: 1107 4117
BseYI C'CCAG_C
Cuts at: 4427 4923 4427
Size: 496 4728
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2445 1163
Size: 573 709 3942
Fragments arranged by size:
3942 709 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2674 3435 4463 1540
Size: 275 186 673 761 1028 2301
Fragments arranged by size:
2301 1028 761 673 275 186
BsiHKAI G_wGCw'C
Cuts at: 2106 2419 3285 3370 4441 2106
Size: 313 866 85 1071 2889
Fragments arranged by size:
2889 1071 866 313 85
BsmI GAATG_Cn'
Cuts at: 2735 2828 2735
Size: 93 5131
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2106 2380 2419 2543 3285 3370 4441
Size: 399 316 274 39 124 742 85 1071
Cuts at: 4441 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 316 274 124 85
39
BspEI T'CCGG_A
Cuts at: 2154 4887 2154
Size: 2733 2491
BspHI T'CATG_A
Cuts at: 2323 3113 4843 2323
Size: 790 1730 2704
Fragments arranged by size:
2704 1730 790
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2127 2579 844
Size: 1283 452 3489
Fragments arranged by size:
3489 1283 452
BsrBI CCG'CTC
Cuts at: 1394 1823 3111 1394
Size: 429 1288 3507
Fragments arranged by size:
3507 1288 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3719 3893 65
Size: 1183 2471 174 1396
Fragments arranged by size:
2471 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3868 4055 1700
Size: 110 2058 187 2869
Fragments arranged by size:
2869 2058 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5224
BssHII G'CGCG_C
Cuts at: 1925 2164 1925
Size: 239 4985
BssSI C'ACGA_G
Cuts at: 2261 3284 4296 2261
Size: 1023 1012 3189
Fragments arranged by size:
3189 1023 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2096 794
Size: 1302 3922
BstBI TT'CG_AA
Cuts at: 4028 4028
Size: 5224
BstEII G'GTnAC_C
Cuts at: 1184 2146 1184
Size: 962 4262
BstYI r'GATC_y
Cuts at: 1972 2554 2921 3306 3323 4775 4861 4873 5219
Size: 582 367 385 17 1452 86 12 346
Cuts at: 5219 1972
Size: 1977
Fragments arranged by size:
1977 1452 582 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2210 2366 2512 513
Size: 552 939 206 156 146 3225
Fragments arranged by size:
3225 939 552 206 156 146
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2506 487
Size: 683 56 1280 3205
Fragments arranged by size:
3205 1280 683 56
BtsI GCAGTG_nn'
Cuts at: 2740 3534 3554 2740
Size: 794 20 4410
Fragments arranged by size:
4410 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5224
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5189
DraI TTT'AAA
Cuts at: 2528 2528
Size: 5224
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4231 809
Size: 507 2915 1802
Fragments arranged by size:
2915 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2671 3560 5189
Size: 54 1425 50 275 18 841 889 1629
Cuts at: 5189 8
Size: 43
Fragments arranged by size:
1629 1425 889 841 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2671 1537
Size: 275 859 4090
Fragments arranged by size:
4090 859 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3154 1353
Size: 1801 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2364 3811 4185 4331 387
Size: 1064 138 775 1447 374 146 1280
Fragments arranged by size:
1447 1280 1064 775 374 146 138
EcoICRI GAG'CTC
Cuts at: 2104 2104
Size: 5224
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2133 3287 3884 4671 4939 1239
Size: 393 501 1154 597 787 268 1524
Fragments arranged by size:
1524 1154 787 597 501 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2027 2027
Size: 5224
EcoRI G'AATT_C
Cuts at: 2533 2911 2533
Size: 378 4846
FspI TGC'GCA
Cuts at: 2135 3730 2135
Size: 1595 3629
HaeII r_GCGC'y
Cuts at: 1678 2062 2237 2246 2341 4371 1678
Size: 384 175 9 95 2030 2531
Fragments arranged by size:
2531 2030 384 175 95 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2272 2304 3865 3897 3939
Size: 32 135 32 700 32 1561 32 42
Cuts at: 3939 3971 1373
Size: 32 2626
Fragments arranged by size:
2626 1561 700 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2568 2814 669
Size: 988 281 630 246 3079
Fragments arranged by size:
3079 988 630 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5224
HpaI GTT'AAC
Cuts at: 2814 2814
Size: 5224
KasI G'GCGC_C
Cuts at: 2242 2242
Size: 5224
KpnI G_GTAC'C
Cuts at: 2549 2549
Size: 5224
MfeI C'AATT_G
Cuts at: 2823 2823
Size: 5224
MluI A'CGCG_T
Cuts at: 5100 5100
Size: 5224
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4338 4522 1305
Size: 3033 184 2007
Fragments arranged by size:
3033 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3402
Fragments arranged by size:
3402 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2328 2451 3182 3541 3700 518
Size: 648 1162 123 731 359 159 2042
Fragments arranged by size:
2042 1162 731 648 359 159 123
MspA1I CmG'CkG
Cuts at: 1606 2093 2212 2252 2640 3317 4465 4710 1606
Size: 487 119 40 388 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 487 388 245 119 40
NarI GG'CG_CC
Cuts at: 2243 2243
Size: 5224
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3733
Fragments arranged by size:
3733 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5224
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5224
NotI GC'GGCC_GC
Cuts at: 2671 2671
Size: 5224
NspI r_CATG'y
Cuts at: 1132 1229 2582 4127 1132
Size: 97 1353 1545 2229
Fragments arranged by size:
2229 1545 1353 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5224
PciI A'CATG_T
Cuts at: 4123 4123
Size: 5224
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5224
PfoI T'CCnGG_A
Cuts at: 2440 2440
Size: 5224
PmeI GTTT'AAAC
Cuts at: 2528 2528
Size: 5224
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3293 3325 4806 4838 3293
Size: 32 1481 32 3679
Fragments arranged by size:
3679 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2027 2027
Size: 5224
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5224
PsiI TTA'TAA
Cuts at: 2794 2794
Size: 5224
PstI C_TGCA'G
Cuts at: 830 2040 2576 830
Size: 1210 536 3478
Fragments arranged by size:
3478 1210 536
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5224
SacI G_AGCT'C
Cuts at: 2106 2106
Size: 5224
SacII CC_GC'GG
Cuts at: 2213 2213
Size: 5224
SalI G'TCGA_C
Cuts at: 1936 2566 1936
Size: 630 4594
SanDI GG'GwC_CC
Cuts at: 2027 2027
Size: 5224
SbfI CC_TGCA'GG
Cuts at: 2576 2576
Size: 5224
ScaI AGT'ACT
Cuts at: 3472 5184 3472
Size: 1712 3512
SexAI A'CCwGG_T
Cuts at: 2317 2317
Size: 5224
SfcI C'TryA_G
Cuts at: 826 1045 2036 2572 3707 4388 4579 826
Size: 219 991 536 1135 681 191 1471
Fragments arranged by size:
1471 1135 991 681 536 219 191
SfoI GGC'GCC
Cuts at: 2244 2244
Size: 5224
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5224
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5224
SmaI CCC'GGG
Cuts at: 1872 2430 2551 1872
Size: 558 121 4545
Fragments arranged by size:
4545 558 121
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3328 4229 4491 820
Size: 197 926 1385 901 262 1553
Fragments arranged by size:
1553 1385 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3015 493
Size: 2522 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5224
SphI G_CATG'C
Cuts at: 1132 2582 1132
Size: 1450 3774
SspI AAT'ATT
Cuts at: 5 52 3148 5
Size: 47 3096 2081
Fragments arranged by size:
3096 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5224
StyI C'CwwG_G
Cuts at: 513 1065 2004 2663 513
Size: 552 939 659 3074
Fragments arranged by size:
3074 939 659 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3251 3268 3421 3606 4051 325
Size: 1255 1671 17 153 185 445 1498
Fragments arranged by size:
1671 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3470 5182 96
Size: 274 80 33 51 1443 1493 1712 138
Fragments arranged by size:
1712 1493 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5184 5216 5184
Size: 32 5192
Tth111I GACn'n_nGTC
Cuts at: 1316 2319 1316
Size: 1003 4221
XbaI T'CTAG_A
Cuts at: 2560 2560
Size: 5224
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5224
XmaI C'CCGG_G
Cuts at: 1870 2428 2549 1870
Size: 558 121 4545
Fragments arranged by size:
4545 558 121
XmnI GAAnn'nnTTC
Cuts at: 3353 3353
Size: 5224
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3032 276
Size: 53 83 186 936 1498 2468
Fragments arranged by size:
2468 1498 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI SacI SacII SalI SanDI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII
RsrII SapI SfiI SrfI SwaI XcmI