Restriction map for
pFN21AB8878
(Circular) (Six-base) MAPSORT of: pFN21AB8878.seq Check: 1928 from: 1 to: 5212
pFN21AB8878 5212 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3022 278
Size: 53 83 186 936 1486 2468
Fragments arranged by size:
2468 1486 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2555 1937
Size: 618 4594
Acc65I G'GTAC_C
Cuts at: 2111 2533 2111
Size: 422 4790
AclI AA'CG_TT
Cuts at: 1563 3339 3712 1563
Size: 1776 373 3063
Fragments arranged by size:
3063 1776 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2079 2430 3275 4659 4927 1632
Size: 447 351 845 1384 268 1917
Fragments arranged by size:
1917 1384 845 447 351 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5015
AflIII A'CryG_T
Cuts at: 1203 4111 5088 1203
Size: 2908 977 1327
Fragments arranged by size:
2908 1327 977
AgeI A'CCGG_T
Cuts at: 4043 4043
Size: 5212
AhdI GACnn_n'nnGTC
Cuts at: 3941 3941
Size: 5212
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5212
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5212
AlwNI CAG_nnn'CTG
Cuts at: 2955 4527 2955
Size: 1572 3640
ApaLI G'TGCA_C
Cuts at: 3269 4425 3269
Size: 1156 4056
ApoI r'AATT_y
Cuts at: 1493 2521 2747 2899 5008 1493
Size: 1028 226 152 2109 1697
Fragments arranged by size:
2109 1697 1028 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3766 160
Size: 872 2734 1606
Fragments arranged by size:
2734 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5212
AvaI C'yCGr_G
Cuts at: 1870 1943 2343 2537 1870
Size: 73 400 194 4545
Fragments arranged by size:
4545 400 194 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2103 2136 2103
Size: 33 5179
BamHI G'GATC_C
Cuts at: 2542 2909 2542
Size: 367 4845
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2111 2533 3988 618
Size: 325 217 627 324 422 1455 1842
Fragments arranged by size:
1842 1455 627 422 325 324 217
BanII G_rGCy'C
Cuts at: 1391 2243 2270 2479 2531 1391
Size: 852 27 209 52 4072
Fragments arranged by size:
4072 852 209 52 27
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2085 2301 928
Size: 971 186 216 3839
Fragments arranged by size:
3839 971 216 186
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3403 3437 1903
Size: 34 1466 34 3678
Fragments arranged by size:
3678 1466 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3104 4314 3104
Size: 1210 4002
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5212
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3823 136
Size: 107 122 71 631 605 2151 1525
Fragments arranged by size:
2151 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5207 5207
Size: 5212
BlpI GC'TnA_GC
Cuts at: 2629 2629
Size: 5212
Bme1580I G_kGCm'C
Cuts at: 1790 3273 4429 1790
Size: 1483 1156 2573
Fragments arranged by size:
2573 1483 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5212
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2943 3901 442
Size: 416 53 83 399 24 1526 958 1753
Fragments arranged by size:
1753 1526 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2408 1056
Size: 1352 3860
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2235 3872 1239
Size: 996 1637 2579
Fragments arranged by size:
2579 1637 996
Bpu10I CC'TnA_GC
Cuts at: 2277 2277
Size: 5212
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2411 3337 4202 4500 2411
Size: 926 865 298 3123
Fragments arranged by size:
3123 926 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3875 882
Size: 617 2376 2219
Fragments arranged by size:
2376 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3003 493
Size: 711 1799 2702
Fragments arranged by size:
2702 1799 711
BsaBI GATnn'nnATC
Cuts at: 5206 5206
Size: 5212
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3019 3401 4036 275
Size: 53 83 186 936 1486 382 635 1451
Fragments arranged by size:
1486 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3645 4043 4317 4464 4875 3645
Size: 398 274 147 411 3982
Fragments arranged by size:
3982 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4987
Fragments arranged by size:
4987 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2213 2216 2303 1186
Size: 1027 3 87 4095
Fragments arranged by size:
4095 1027 87 3
BseYI C'CCAG_C
Cuts at: 2049 2338 4415 4911 2049
Size: 289 2077 496 2350
Fragments arranged by size:
2350 2077 496 289
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2486 1163
Size: 573 750 3889
Fragments arranged by size:
3889 750 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2662 3423 4451 1540
Size: 275 186 661 761 1028 2301
Fragments arranged by size:
2301 1028 761 661 275 186
BsiHKAI G_wGCw'C
Cuts at: 2270 2479 3273 3358 4429 2270
Size: 209 794 85 1071 3053
Fragments arranged by size:
3053 1071 794 209 85
BsmI GAATG_Cn'
Cuts at: 2723 2816 2723
Size: 93 5119
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2243 2270 2479 2531 3273 3358 4429
Size: 399 453 27 209 52 742 85 1071
Cuts at: 4429 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 453 399 209 85 52
27
BspEI T'CCGG_A
Cuts at: 4875 4875
Size: 5212
BspHI T'CATG_A
Cuts at: 3101 4831 3101
Size: 1730 3482
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2085 2567 844
Size: 1241 482 3489
Fragments arranged by size:
3489 1241 482
BsrBI CCG'CTC
Cuts at: 1394 1823 3099 1394
Size: 429 1276 3507
Fragments arranged by size:
3507 1276 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2018 3707 3881 65
Size: 1183 770 1689 174 1396
Fragments arranged by size:
1689 1396 1183 770 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3856 4043 1700
Size: 110 2046 187 2869
Fragments arranged by size:
2869 2046 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5212
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5212
BssSI C'ACGA_G
Cuts at: 3272 4284 3272
Size: 1012 4200
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5212
BstBI TT'CG_AA
Cuts at: 4016 4016
Size: 5212
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5212
BstYI r'GATC_y
Cuts at: 1972 2542 2909 3294 3311 4763 4849 4861 5207
Size: 570 367 385 17 1452 86 12 346
Cuts at: 5207 1972
Size: 1977
Fragments arranged by size:
1977 1452 570 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3721
Fragments arranged by size:
3721 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4473
Fragments arranged by size:
4473 683 56
BtsI GCAGTG_nn'
Cuts at: 2728 3522 3542 2728
Size: 794 20 4398
Fragments arranged by size:
4398 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5212
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5177
DraI TTT'AAA
Cuts at: 2516 2516
Size: 5212
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4219 809
Size: 507 2903 1802
Fragments arranged by size:
2903 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2659 3548 5177
Size: 54 1425 50 275 18 829 889 1629
Cuts at: 5177 8
Size: 43
Fragments arranged by size:
1629 1425 889 829 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2659 1537
Size: 275 847 4090
Fragments arranged by size:
4090 847 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2133 2280 2406 3142 1353
Size: 780 147 126 736 3423
Fragments arranged by size:
3423 780 736 147 126
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3799 4173 4319 387
Size: 1064 138 2210 374 146 1280
Fragments arranged by size:
2210 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2268 2477 2268
Size: 209 5003
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2079 2235 2430 3275 3872 4659 4927
Size: 393 447 156 195 845 597 787 268
Cuts at: 4927 1239
Size: 1524
Fragments arranged by size:
1524 845 787 597 447 393 268 195
156
EcoNI CCTnn'n_nnAGG
Cuts at: 2081 2276 2081
Size: 195 5017
EcoO109I rG'GnC_Cy
Cuts at: 2074 2086 2074
Size: 12 5200
EcoRI G'AATT_C
Cuts at: 2521 2899 2521
Size: 378 4834
FspI TGC'GCA
Cuts at: 3718 3718
Size: 5212
HaeII r_GCGC'y
Cuts at: 1678 2446 4359 1678
Size: 768 1913 2531
Fragments arranged by size:
2531 1913 768
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2075 2107 2132 2164 3853
Size: 32 135 32 503 32 25 32 1689
Cuts at: 3853 3885 3927 3959 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1689 503 135 42 32 32 32
32 32 32 25
HincII GTy'rAC
Cuts at: 669 1657 1938 2556 2802 669
Size: 988 281 618 246 3079
Fragments arranged by size:
3079 988 618 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5212
HpaI GTT'AAC
Cuts at: 2802 2802
Size: 5212
KpnI G_GTAC'C
Cuts at: 2115 2537 2115
Size: 422 4790
MfeI C'AATT_G
Cuts at: 2811 2811
Size: 5212
MluI A'CGCG_T
Cuts at: 5088 5088
Size: 5212
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4326 4510 1305
Size: 3021 184 2007
Fragments arranged by size:
3021 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3390
Fragments arranged by size:
3390 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3170 3529 3688 518
Size: 648 2004 359 159 2042
Fragments arranged by size:
2042 2004 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2012 2049 2628 3305 4453 4698 1606
Size: 406 37 579 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 579 406 245 37
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3721
Fragments arranged by size:
3721 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5212
NheI G'CTAG_C
Cuts at: 1052 2404 1052
Size: 1352 3860
NotI GC'GGCC_GC
Cuts at: 2659 2659
Size: 5212
NspI r_CATG'y
Cuts at: 1132 1229 2570 4115 1132
Size: 97 1341 1545 2229
Fragments arranged by size:
2229 1545 1341 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5212
PciI A'CATG_T
Cuts at: 4111 4111
Size: 5212
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5212
PfoI T'CCnGG_A
Cuts at: 2128 2128
Size: 5212
PmeI GTTT'AAAC
Cuts at: 2516 2516
Size: 5212
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3281 3313 4794 4826 3281
Size: 32 1481 32 3667
Fragments arranged by size:
3667 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2074 2086 2074
Size: 12 5200
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5212
PsiI TTA'TAA
Cuts at: 2782 2782
Size: 5212
PstI C_TGCA'G
Cuts at: 830 2564 830
Size: 1734 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5212
PvuII CAG'CTG
Cuts at: 2012 2049 2012
Size: 37 5175
SacI G_AGCT'C
Cuts at: 2270 2479 2270
Size: 209 5003
SalI G'TCGA_C
Cuts at: 1936 2554 1936
Size: 618 4594
SbfI CC_TGCA'GG
Cuts at: 2564 2564
Size: 5212
ScaI AGT'ACT
Cuts at: 3460 5172 3460
Size: 1712 3500
SfcI C'TryA_G
Cuts at: 826 1045 2560 3695 4376 4567 826
Size: 219 1515 1135 681 191 1471
Fragments arranged by size:
1515 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5212
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5212
SmaI CCC'GGG
Cuts at: 1872 2345 2539 1872
Size: 473 194 4545
Fragments arranged by size:
4545 473 194
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2426 3316 4217 4479 820
Size: 197 926 483 890 901 262 1553
Fragments arranged by size:
1553 926 901 890 483 262 197
SnaBI TAC'GTA
Cuts at: 493 3003 493
Size: 2510 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5212
SphI G_CATG'C
Cuts at: 1132 2570 1132
Size: 1438 3774
SrfI GCCC'GGGC
Cuts at: 2345 2345
Size: 5212
SspI AAT'ATT
Cuts at: 5 52 3136 5
Size: 47 3084 2081
Fragments arranged by size:
3084 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5212
StyI C'CwwG_G
Cuts at: 513 1065 2004 2299 2651 513
Size: 552 939 295 352 3074
Fragments arranged by size:
3074 939 552 352 295
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2502 3239 3256 3409 3594 4039 325
Size: 1255 922 737 17 153 185 445 1498
Fragments arranged by size:
1498 1255 922 737 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3458 5170 96
Size: 274 80 33 51 1443 1481 1712 138
Fragments arranged by size:
1712 1481 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2311 2343 5172 5204 2311
Size: 32 2829 32 2319
Fragments arranged by size:
2829 2319 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2041 1316
Size: 725 4487
XbaI T'CTAG_A
Cuts at: 2548 2548
Size: 5212
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2053 2053
Size: 5212
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5212
XmaI C'CCGG_G
Cuts at: 1870 2343 2537 1870
Size: 473 194 4545
Fragments arranged by size:
4545 473 194
XmnI GAAnn'nnTTC
Cuts at: 3341 3341
Size: 5212
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3020 276
Size: 53 83 186 936 1486 2468
Fragments arranged by size:
2468 1486 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII
SacII SanDI SapI SexAI SfiI SfoI SwaI