Restriction map for
pFN21AB8850
(Circular) (Six-base) MAPSORT of: pFN21AB8850.seq Check: 5434 from: 1 to: 4918
pFN21AB8850 4918 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2728 278
Size: 53 83 186 936 1192 2468
Fragments arranged by size:
2468 1192 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2261 1937
Size: 324 4594
Acc65I G'GTAC_C
Cuts at: 2239 2239
Size: 4918
AclI AA'CG_TT
Cuts at: 1563 3045 3418 1563
Size: 1482 373 3063
Fragments arranged by size:
3063 1482 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2157 2981 4365 4633 1632
Size: 525 824 1384 268 1917
Fragments arranged by size:
1917 1384 824 525 268
AflII C'TTAA_G
Cuts at: 820 1017 2060 820
Size: 197 1043 3678
Fragments arranged by size:
3678 1043 197
AflIII A'CryG_T
Cuts at: 1203 3817 4794 1203
Size: 2614 977 1327
Fragments arranged by size:
2614 1327 977
AgeI A'CCGG_T
Cuts at: 3749 3749
Size: 4918
AhdI GACnn_n'nnGTC
Cuts at: 3647 3647
Size: 4918
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 4918
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 4918
AlwNI CAG_nnn'CTG
Cuts at: 2661 4233 2661
Size: 1572 3346
ApaLI G'TGCA_C
Cuts at: 2975 4131 2975
Size: 1156 3762
ApoI r'AATT_y
Cuts at: 1493 2227 2453 2605 4714 1493
Size: 734 226 152 2109 1697
Fragments arranged by size:
2109 1697 734 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3472 160
Size: 872 2440 1606
Fragments arranged by size:
2440 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4918
AvaI C'yCGr_G
Cuts at: 1870 1943 2243 1870
Size: 73 300 4545
Fragments arranged by size:
4545 300 73
BamHI G'GATC_C
Cuts at: 2248 2615 2248
Size: 367 4551
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2239 3694 618
Size: 325 217 627 452 1455 1842
Fragments arranged by size:
1842 1455 627 452 325 217
BanII G_rGCy'C
Cuts at: 1391 2237 1391
Size: 846 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2151 928
Size: 971 252 3695
Fragments arranged by size:
3695 971 252
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3109 3143 1903
Size: 34 1172 34 3678
Fragments arranged by size:
3678 1172 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2810 4020 2810
Size: 1210 3708
BclI T'GATC_A
Cuts at: 1548 1548
Size: 4918
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3529 136
Size: 107 122 71 631 605 1857 1525
Fragments arranged by size:
1857 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4913 4913
Size: 4918
BlpI GC'TnA_GC
Cuts at: 2335 2335
Size: 4918
Bme1580I G_kGCm'C
Cuts at: 1790 2979 4135 1790
Size: 1189 1156 2573
Fragments arranged by size:
2573 1189 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 4918
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2649 3607 442
Size: 416 53 83 399 24 1232 958 1753
Fragments arranged by size:
1753 1232 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 4918
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3578 1239
Size: 2339 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3043 3908 4206 3043
Size: 865 298 3755
Fragments arranged by size:
3755 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3581 882
Size: 617 2082 2219
Fragments arranged by size:
2219 2082 617
BsaAI yAC'GTr
Cuts at: 493 1204 2709 493
Size: 711 1505 2702
Fragments arranged by size:
2702 1505 711
BsaBI GATnn'nnATC
Cuts at: 4912 4912
Size: 4918
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2725 3107 3742 275
Size: 53 83 186 936 1192 382 635 1451
Fragments arranged by size:
1451 1192 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3351 3749 4023 4170 4581 3351
Size: 398 274 147 411 3688
Fragments arranged by size:
3688 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2183 2213 1178
Size: 30 165 30 780 30 3883
Fragments arranged by size:
3883 780 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 4918
BseYI C'CCAG_C
Cuts at: 4121 4617 4121
Size: 496 4422
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4345
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2368 3129 4157 1540
Size: 275 186 367 761 1028 2301
Fragments arranged by size:
2301 1028 761 367 275 186
BsiHKAI G_wGCw'C
Cuts at: 2979 3064 4135 2979
Size: 85 1071 3762
Fragments arranged by size:
3762 1071 85
BsmI GAATG_Cn'
Cuts at: 2429 2522 2429
Size: 93 4825
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2237 2979 3064 4135 1391
Size: 399 447 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 447 399 85
BspEI T'CCGG_A
Cuts at: 4581 4581
Size: 4918
BspHI T'CATG_A
Cuts at: 2807 4537 2807
Size: 1730 3188
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2273 844
Size: 1429 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2805 1394
Size: 429 982 3507
Fragments arranged by size:
3507 982 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3413 3587 65
Size: 1183 2165 174 1396
Fragments arranged by size:
2165 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3562 3749 1700
Size: 110 1752 187 2869
Fragments arranged by size:
2869 1752 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 4918
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 4918
BssSI C'ACGA_G
Cuts at: 2978 3990 2978
Size: 1012 3906
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 4918
BstBI TT'CG_AA
Cuts at: 3722 3722
Size: 4918
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 4918
BstXI CCAn_nnnn'nTGG
Cuts at: 2047 2047
Size: 4918
BstYI r'GATC_y
Cuts at: 1972 2248 2615 3000 3017 4469 4555 4567 4913
Size: 276 367 385 17 1452 86 12 346
Cuts at: 4913 1972
Size: 1977
Fragments arranged by size:
1977 1452 385 367 346 276 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3427
Fragments arranged by size:
3427 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4179
Fragments arranged by size:
4179 683 56
BtsI GCAGTG_nn'
Cuts at: 2434 3228 3248 2434
Size: 794 20 4104
Fragments arranged by size:
4104 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 4918
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 4883
DraI TTT'AAA
Cuts at: 2222 2222
Size: 4918
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3925 809
Size: 507 2609 1802
Fragments arranged by size:
2609 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2365 3254
Size: 54 1425 50 275 18 177 358 889
Cuts at: 3254 4883 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 358 275 177 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2365 1537
Size: 275 553 4090
Fragments arranged by size:
4090 553 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2848 1353
Size: 1495 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3505 3879 4025 387
Size: 1064 138 1916 374 146 1280
Fragments arranged by size:
1916 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2157 2981 3578 4365 4633 1239
Size: 393 525 824 597 787 268 1524
Fragments arranged by size:
1524 824 787 597 525 393 268
EcoRI G'AATT_C
Cuts at: 2227 2605 2227
Size: 378 4540
FspI TGC'GCA
Cuts at: 3424 3424
Size: 4918
HaeII r_GCGC'y
Cuts at: 1678 4065 1678
Size: 2387 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3559 3591 3633 3665 1373
Size: 32 135 32 1987 32 42 32 2626
Fragments arranged by size:
2626 1987 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2262 2508 669
Size: 988 281 324 246 3079
Fragments arranged by size:
3079 988 324 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 4918
HpaI GTT'AAC
Cuts at: 2508 2508
Size: 4918
KpnI G_GTAC'C
Cuts at: 2243 2243
Size: 4918
MfeI C'AATT_G
Cuts at: 2517 2517
Size: 4918
MluI A'CGCG_T
Cuts at: 4794 4794
Size: 4918
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4032 4216 1305
Size: 2727 184 2007
Fragments arranged by size:
2727 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 10
Size: 54 1425 343 177 2919
Fragments arranged by size:
2919 1425 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2876 3235 3394 518
Size: 648 1710 359 159 2042
Fragments arranged by size:
2042 1710 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2105 2334 3011 4159 4404 1606
Size: 499 229 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 499 245 229
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3427
Fragments arranged by size:
3427 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 4918
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 4918
NotI GC'GGCC_GC
Cuts at: 2365 2365
Size: 4918
NspI r_CATG'y
Cuts at: 1132 1229 2138 2276 3821 1132
Size: 97 909 138 1545 2229
Fragments arranged by size:
2229 1545 909 138 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 4918
PciI A'CATG_T
Cuts at: 3817 3817
Size: 4918
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 4918
PmeI GTTT'AAAC
Cuts at: 2222 2222
Size: 4918
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2987 3019 4500 4532 2987
Size: 32 1481 32 3373
Fragments arranged by size:
3373 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 4918
PsiI TTA'TAA
Cuts at: 2488 2488
Size: 4918
PstI C_TGCA'G
Cuts at: 830 2110 2270 830
Size: 1280 160 3478
Fragments arranged by size:
3478 1280 160
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 4918
PvuII CAG'CTG
Cuts at: 2105 2105
Size: 4918
SalI G'TCGA_C
Cuts at: 1936 2260 1936
Size: 324 4594
SbfI CC_TGCA'GG
Cuts at: 2270 2270
Size: 4918
ScaI AGT'ACT
Cuts at: 3166 4878 3166
Size: 1712 3206
SfcI C'TryA_G
Cuts at: 826 1045 2106 2266 3401 4082 4273 826
Size: 219 1061 160 1135 681 191 1471
Fragments arranged by size:
1471 1135 1061 681 219 191 160
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4918
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 4918
SmaI CCC'GGG
Cuts at: 1872 2245 1872
Size: 373 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2060 3022 3923 4185 820
Size: 197 926 117 962 901 262 1553
Fragments arranged by size:
1553 962 926 901 262 197 117
SnaBI TAC'GTA
Cuts at: 493 2709 493
Size: 2216 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 4918
SphI G_CATG'C
Cuts at: 1132 2276 1132
Size: 1144 3774
SspI AAT'ATT
Cuts at: 5 52 2842 5
Size: 47 2790 2081
Fragments arranged by size:
2790 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 4918
StyI C'CwwG_G
Cuts at: 513 1065 2004 2097 2139 2357 513
Size: 552 939 93 42 218 3074
Fragments arranged by size:
3074 939 552 218 93 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2945 2962 3115 3300 3745 325
Size: 1255 1365 17 153 185 445 1498
Fragments arranged by size:
1498 1365 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3164 4876 96
Size: 274 80 33 51 1443 1187 1712 138
Fragments arranged by size:
1712 1443 1187 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4878 4910 4878
Size: 32 4886
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 4918
XbaI T'CTAG_A
Cuts at: 2254 2254
Size: 4918
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 4918
XmaI C'CCGG_G
Cuts at: 1870 2243 1870
Size: 373 4545
XmnI GAAnn'nnTTC
Cuts at: 2201 3047 2201
Size: 846 4072
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2726 276
Size: 53 83 186 936 1192 2468
Fragments arranged by size:
2468 1192 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI