Restriction map for
pFN21AB8846
(Circular) (Six-base) MAPSORT of: pFN21AB8846.seq Check: 1146 from: 1 to: 6310
pFN21AB8846 6310 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3481 3481
Size: 6310
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4120 278
Size: 53 83 186 936 2584 2468
Fragments arranged by size:
2584 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2672 2811 3536 3653 1937
Size: 735 139 725 117 4594
Fragments arranged by size:
4594 735 725 139 117
Acc65I G'GTAC_C
Cuts at: 3631 3631
Size: 6310
AclI AA'CG_TT
Cuts at: 1563 4437 4810 1563
Size: 2874 373 3063
Fragments arranged by size:
3063 2874 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2597 2965 4373 5757 6025 1632
Size: 965 368 1408 1384 268 1917
Fragments arranged by size:
1917 1408 1384 965 368 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6113
AflIII A'CryG_T
Cuts at: 1203 2626 3173 5209 6186 1203
Size: 1423 547 2036 977 1327
Fragments arranged by size:
2036 1423 1327 977 547
AgeI A'CCGG_T
Cuts at: 5141 5141
Size: 6310
AhdI GACnn_n'nnGTC
Cuts at: 2539 5039 2539
Size: 2500 3810
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6310
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6310
AlwNI CAG_nnn'CTG
Cuts at: 3511 3562 4053 5625 3511
Size: 51 491 1572 4196
Fragments arranged by size:
4196 1572 491 51
ApaLI G'TGCA_C
Cuts at: 4367 5523 4367
Size: 1156 5154
ApoI r'AATT_y
Cuts at: 1493 3037 3619 3845 3997 6106 1493
Size: 1544 582 226 152 2109 1697
Fragments arranged by size:
2109 1697 1544 582 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4864 160
Size: 872 3832 1606
Fragments arranged by size:
3832 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6310
AvaI C'yCGr_G
Cuts at: 1870 1943 2494 2792 2902 3635 1870
Size: 73 551 298 110 733 4545
Fragments arranged by size:
4545 733 551 298 110 73
BamHI G'GATC_C
Cuts at: 3640 4007 3640
Size: 367 5943
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2876 3631 5086 618
Size: 325 217 627 1089 755 1455 1842
Fragments arranged by size:
1842 1455 1089 755 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2297 2359 2585 2903 3629 1391
Size: 906 62 226 318 726 4072
Fragments arranged by size:
4072 906 726 318 226 62
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5339
BbvCI CC'TCA_GC
Cuts at: 2326 2326
Size: 6310
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4501 4535 1903
Size: 34 2564 34 3678
Fragments arranged by size:
3678 2564 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2709 4202 5412 2709
Size: 1493 1210 3607
Fragments arranged by size:
3607 1493 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6310
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2422 3011 4921
Size: 107 122 71 631 605 750 589 1910
Cuts at: 4921 136
Size: 1525
Fragments arranged by size:
1910 1525 750 631 605 589 122 107
71
BglII A'GATC_T
Cuts at: 2838 6305 2838
Size: 3467 2843
BlpI GC'TnA_GC
Cuts at: 3727 3727
Size: 6310
Bme1580I G_kGCm'C
Cuts at: 1790 2282 4371 5527 1790
Size: 492 2089 1156 2573
Fragments arranged by size:
2573 2089 1156 492
BmgBI CAC'GTC
Cuts at: 1318 2350 2379 1318
Size: 1032 29 5249
Fragments arranged by size:
5249 1032 29
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3169 3467 4041
Size: 416 53 83 399 24 1752 298 574
Cuts at: 4041 4999 442
Size: 958 1753
Fragments arranged by size:
1753 1752 958 574 416 399 298 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2102 3200 1056
Size: 1046 1098 4166
Fragments arranged by size:
4166 1098 1046
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2522 4970 1239
Size: 1283 2448 2579
Fragments arranged by size:
2579 2448 1283
Bpu10I CC'TnA_GC
Cuts at: 2326 2326
Size: 6310
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2754 3283 4435 5300 5598 2754
Size: 529 1152 865 298 3466
Fragments arranged by size:
3466 1152 865 529 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2435 2540 2595 3432 4973 882
Size: 617 936 105 55 837 1541 2219
Fragments arranged by size:
2219 1541 936 837 617 105 55
BsaAI yAC'GTr
Cuts at: 493 1204 2553 3010 4101 493
Size: 711 1349 457 1091 2702
Fragments arranged by size:
2702 1349 1091 711 457
BsaBI GATnn'nnATC
Cuts at: 6304 6304
Size: 6310
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2514 4117 4499 5134
Size: 53 83 186 936 981 1603 382 635
Cuts at: 5134 275
Size: 1451
Fragments arranged by size:
1603 1451 981 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2517 4743 5141 5415 5562 5973 2517
Size: 2226 398 274 147 411 2854
Fragments arranged by size:
2854 2226 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2300 2330 2710 2740 1178
Size: 30 165 30 897 30 380 30 4748
Fragments arranged by size:
4748 897 380 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2308 2311 2314 2453 2757 3140 1186
Size: 1122 3 3 139 304 383 4356
Fragments arranged by size:
4356 1122 383 304 139 3 3
BseYI C'CCAG_C
Cuts at: 5513 6009 5513
Size: 496 5814
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5737
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3247 3760 4521 5549 1540
Size: 275 186 1246 513 761 1028 2301
Fragments arranged by size:
2301 1246 1028 761 513 275 186
BsiHKAI G_wGCw'C
Cuts at: 2297 2359 2585 3309 4371 4456 5527 2297
Size: 62 226 724 1062 85 1071 3080
Fragments arranged by size:
3080 1071 1062 724 226 85 62
BsmI GAATG_Cn'
Cuts at: 3382 3821 3914 3382
Size: 439 93 5778
Fragments arranged by size:
5778 439 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2282 2297 2359 2585 2903 3309 3629
Size: 399 492 15 62 226 318 406 320
Cuts at: 3629 4371 4456 5527 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 492 406 399 320 318
226 85 62 15
BspEI T'CCGG_A
Cuts at: 2517 5973 2517
Size: 3456 2854
BspHI T'CATG_A
Cuts at: 4199 5929 4199
Size: 1730 4580
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2453 2613 3481 3665 844
Size: 1609 160 868 184 3489
Fragments arranged by size:
3489 1609 868 184 160
BsrBI CCG'CTC
Cuts at: 1394 1823 2909 4197 1394
Size: 429 1086 1288 3507
Fragments arranged by size:
3507 1288 1086 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2939 4805 4979 65
Size: 1183 1691 1866 174 1396
Fragments arranged by size:
1866 1691 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4954 5141 1700
Size: 110 3144 187 2869
Fragments arranged by size:
3144 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6310
BssHII G'CGCG_C
Cuts at: 1925 2782 1925
Size: 857 5453
BssSI C'ACGA_G
Cuts at: 4370 5382 4370
Size: 1012 5298
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6310
BstBI TT'CG_AA
Cuts at: 5114 5114
Size: 6310
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6310
BstYI r'GATC_y
Cuts at: 1972 2838 3440 3640 4007 4392 4409 5861 5947
Size: 866 602 200 367 385 17 1452 86
Cuts at: 5947 5959 6305 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 866 602 385 367 346 200
86 17 12
BstZ17I GTA'TAC
Cuts at: 2673 3537 2673
Size: 864 5446
Bsu36I CC'TnA_GG
Cuts at: 2655 2655
Size: 6310
BtgI C'CryG_G
Cuts at: 513 1065 2411 2745 2880 3102 3277 513
Size: 552 1346 334 135 222 175 3546
Fragments arranged by size:
3546 1346 552 334 222 175 135
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2079 487
Size: 683 56 853 4718
Fragments arranged by size:
4718 853 683 56
BtsI GCAGTG_nn'
Cuts at: 3826 4620 4640 3826
Size: 794 20 5496
Fragments arranged by size:
5496 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6310
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6275
DraI TTT'AAA
Cuts at: 2344 3614 2344
Size: 1270 5040
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2825 5317 809
Size: 507 1509 2492 1802
Fragments arranged by size:
2492 1802 1509 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2157 3757 4646
Size: 54 1425 50 275 18 327 1600 889
Cuts at: 4646 6275 8
Size: 1629 43
Fragments arranged by size:
1629 1600 1425 889 327 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3757 1537
Size: 275 1945 4090
Fragments arranged by size:
4090 1945 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2491 2573 2743 4240 1353
Size: 1138 82 170 1497 3423
Fragments arranged by size:
3423 1497 1138 170 82
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2208 4897 5271 5417 387
Size: 1064 138 619 2689 374 146 1280
Fragments arranged by size:
2689 1280 1064 619 374 146 138
EcoICRI GAG'CTC
Cuts at: 2295 2357 2583 2295
Size: 62 226 6022
Fragments arranged by size:
6022 226 62
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2522 2597 2965 4373 4970 5757 6025
Size: 393 890 75 368 1408 597 787 268
Cuts at: 6025 1239
Size: 1524
Fragments arranged by size:
1524 1408 890 787 597 393 368 268
75
EcoNI CCTnn'n_nnAGG
Cuts at: 2706 3122 2706
Size: 416 5894
EcoO109I rG'GnC_Cy
Cuts at: 2818 2818
Size: 6310
EcoRI G'AATT_C
Cuts at: 3619 3997 3619
Size: 378 5932
EcoRV GAT'ATC
Cuts at: 2170 2170
Size: 6310
FspI TGC'GCA
Cuts at: 4816 4816
Size: 6310
HaeII r_GCGC'y
Cuts at: 1678 5457 1678
Size: 3779 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2300 2332 2525 2557 2710
Size: 32 135 32 728 32 193 32 153
Cuts at: 2710 2742 2896 2928 3035 3067 4951 4983 5025
Size: 32 154 32 107 32 1884 32 42
Cuts at: 5025 5057 1373
Size: 32 2626
Fragments arranged by size:
2626 1884 728 193 154 153 135 107
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3654 3900 669
Size: 988 281 1716 246 3079
Fragments arranged by size:
3079 1716 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3259 748
Size: 2511 3799
HpaI GTT'AAC
Cuts at: 3900 3900
Size: 6310
KpnI G_GTAC'C
Cuts at: 3635 3635
Size: 6310
MfeI C'AATT_G
Cuts at: 3909 3909
Size: 6310
MluI A'CGCG_T
Cuts at: 6186 6186
Size: 6310
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5424 5608 1305
Size: 4119 184 2007
Fragments arranged by size:
4119 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2159 10
Size: 54 1425 343 327 4161
Fragments arranged by size:
4161 1425 343 327 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2453 2619 2919 3107 3312 3491 4268
Size: 648 1287 166 300 188 205 179 777
Cuts at: 4268 4627 4786 518
Size: 359 159 2042
Fragments arranged by size:
2042 1287 777 648 359 300 205 188
179 166 159
MspA1I CmG'CkG
Cuts at: 1606 2163 3726 4403 5551 5796 1606
Size: 557 1563 677 1148 245 2120
Fragments arranged by size:
2120 1563 1148 677 557 245
NcoI C'CATG_G
Cuts at: 513 1065 2745 3102 3277 513
Size: 552 1680 357 175 3546
Fragments arranged by size:
3546 1680 552 357 175
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6310
NheI G'CTAG_C
Cuts at: 1052 2098 3196 1052
Size: 1046 1098 4166
Fragments arranged by size:
4166 1098 1046
NotI GC'GGCC_GC
Cuts at: 3757 3757
Size: 6310
NspI r_CATG'y
Cuts at: 1132 1229 2630 3668 5213 1132
Size: 97 1401 1038 1545 2229
Fragments arranged by size:
2229 1545 1401 1038 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6310
PciI A'CATG_T
Cuts at: 2626 5209 2626
Size: 2583 3727
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6310
PmeI GTTT'AAAC
Cuts at: 3614 3614
Size: 6310
PmlI CAC'GTG
Cuts at: 2553 3010 2553
Size: 457 5853
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4379 4411 5892 5924 4379
Size: 32 1481 32 4765
Fragments arranged by size:
4765 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2818 2818
Size: 6310
PshAI GACnn'nnGTC
Cuts at: 1613 3358 1613
Size: 1745 4565
PsiI TTA'TAA
Cuts at: 3880 3880
Size: 6310
PstI C_TGCA'G
Cuts at: 830 3662 830
Size: 2832 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6310
PvuII CAG'CTG
Cuts at: 2163 2163
Size: 6310
SacI G_AGCT'C
Cuts at: 2297 2359 2585 2297
Size: 62 226 6022
Fragments arranged by size:
6022 226 62
SalI G'TCGA_C
Cuts at: 1936 3652 1936
Size: 1716 4594
SapI GCTCTTCn'nnn_
Cuts at: 2573 2743 2573
Size: 170 6140
SbfI CC_TGCA'GG
Cuts at: 3662 3662
Size: 6310
ScaI AGT'ACT
Cuts at: 4558 6270 4558
Size: 1712 4598
SfcI C'TryA_G
Cuts at: 826 1045 2398 3658 4793 5474 5665 826
Size: 219 1353 1260 1135 681 191 1471
Fragments arranged by size:
1471 1353 1260 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2422 2422
Size: 6310
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6310
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6310
SmaI CCC'GGG
Cuts at: 1872 3637 1872
Size: 1765 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2769 3262 4414 5315 5577 820
Size: 197 926 826 493 1152 901 262 1553
Fragments arranged by size:
1553 1152 926 901 826 493 262 197
SnaBI TAC'GTA
Cuts at: 493 4101 493
Size: 3608 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6310
SphI G_CATG'C
Cuts at: 1132 3668 1132
Size: 2536 3774
SspI AAT'ATT
Cuts at: 5 52 4234 5
Size: 47 4182 2081
Fragments arranged by size:
4182 2081 47
StuI AGG'CCT
Cuts at: 1517 2474 1517
Size: 957 5353
StyI C'CwwG_G
Cuts at: 513 1065 2475 2745 3102 3277 3749 513
Size: 552 1410 270 357 175 472 3074
Fragments arranged by size:
3074 1410 552 472 357 270 175
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2347 4337 4354 4507 4692 5137 325
Size: 1255 767 1990 17 153 185 445 1498
Fragments arranged by size:
1990 1498 1255 767 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2083 4556 6268
Size: 274 80 33 51 1443 106 2473 1712
Cuts at: 6268 96
Size: 138
Fragments arranged by size:
2473 1712 1443 274 138 106 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2968 3000 6270 6302 2968
Size: 32 3270 32 2976
Fragments arranged by size:
3270 2976 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6310
XbaI T'CTAG_A
Cuts at: 3646 3646
Size: 6310
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3174 3174
Size: 6310
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6310
XmaI C'CCGG_G
Cuts at: 1870 3635 1870
Size: 1765 4545
XmnI GAAnn'nnTTC
Cuts at: 2525 4439 2525
Size: 1914 4396
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4118 276
Size: 53 83 186 936 2584 2468
Fragments arranged by size:
2584 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI DraIII FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfoI
SrfI SwaI