Restriction map for
pFN21AB8844
(Circular) (Six-base) MAPSORT of: pFN21AB8844.seq Check: 3344 from: 1 to: 6271
pFN21AB8844 6271 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2856 3132 2856
Size: 276 5995
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3063 4081 278
Size: 53 83 186 936 1527 1018 2468
Fragments arranged by size:
2468 1527 1018 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3614 1937
Size: 1677 4594
Acc65I G'GTAC_C
Cuts at: 3592 3592
Size: 6271
AclI AA'CG_TT
Cuts at: 1563 4398 4771 1563
Size: 2835 373 3063
Fragments arranged by size:
3063 2835 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4334 5718 5986 1632
Size: 2702 1384 268 1917
Fragments arranged by size:
2702 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6074
AflIII A'CryG_T
Cuts at: 1203 2967 3345 5170 6147 1203
Size: 1764 378 1825 977 1327
Fragments arranged by size:
1825 1764 1327 977 378
AgeI A'CCGG_T
Cuts at: 5102 5102
Size: 6271
AhdI GACnn_n'nnGTC
Cuts at: 5000 5000
Size: 6271
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6271
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6271
AlwNI CAG_nnn'CTG
Cuts at: 2098 2338 2857 4014 5586 2098
Size: 240 519 1157 1572 2783
Fragments arranged by size:
2783 1572 1157 519 240
ApaI G_GGCC'C
Cuts at: 3162 3162
Size: 6271
ApaLI G'TGCA_C
Cuts at: 4328 5484 4328
Size: 1156 5115
ApoI r'AATT_y
Cuts at: 1493 3580 3806 3958 6067 1493
Size: 2087 226 152 2109 1697
Fragments arranged by size:
2109 2087 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4825 160
Size: 872 3793 1606
Fragments arranged by size:
3793 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6271
AvaI C'yCGr_G
Cuts at: 1870 1943 2056 2651 3596 1870
Size: 73 113 595 945 4545
Fragments arranged by size:
4545 945 595 113 73
BamHI G'GATC_C
Cuts at: 2898 3601 3968 2898
Size: 703 367 5201
Fragments arranged by size:
5201 703 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2118 2182 3296 3592 5047
Size: 325 217 627 331 64 1114 296 1455
Cuts at: 5047 618
Size: 1842
Fragments arranged by size:
1842 1455 1114 627 331 325 296 217
64
BanII G_rGCy'C
Cuts at: 1391 2823 2895 3111 3162 3505 3590 1391
Size: 1432 72 216 51 343 85 4072
Fragments arranged by size:
4072 1432 343 216 85 72 51
BbeI G_GCGC'C
Cuts at: 2186 2186
Size: 6271
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5300
BbvCI CC'TCA_GC
Cuts at: 2045 2045
Size: 6271
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2599 2633 2788 2822 4462 4496 1903
Size: 34 662 34 155 34 1640 34 3678
Fragments arranged by size:
3678 1640 662 155 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2576 4163 5373 2576
Size: 1587 1210 3474
Fragments arranged by size:
3474 1587 1210
BclI T'GATC_A
Cuts at: 1548 3510 1548
Size: 1962 4309
BfrBI ATG'CAT
Cuts at: 3436 3436
Size: 6271
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2208 3173 3236
Size: 107 122 71 631 605 536 965 63
Cuts at: 3236 4882 136
Size: 1646 1525
Fragments arranged by size:
1646 1525 965 631 605 536 122 107
71 63
BglII A'GATC_T
Cuts at: 6266 6266
Size: 6271
BlpI GC'TnA_GC
Cuts at: 3688 3688
Size: 6271
Bme1580I G_kGCm'C
Cuts at: 1790 2123 2535 2977 3162 3299 4332 5488 1790
Size: 333 412 442 185 137 1033 1156 2573
Fragments arranged by size:
2573 1156 1033 442 412 333 185 137
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6271
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2773 4002 4960
Size: 416 53 83 399 24 1356 1229 958
Cuts at: 4960 442
Size: 1753
Fragments arranged by size:
1753 1356 1229 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6271
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3544 3544
Size: 6271
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2798 4931 1239
Size: 1559 2133 2579
Fragments arranged by size:
2579 2133 1559
Bpu10I CC'TnA_GC
Cuts at: 2045 2045
Size: 6271
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2549 2579 4396 5261 5559 2549
Size: 30 1817 865 298 3261
Fragments arranged by size:
3261 1817 865 298 30
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2030 2828 4934 882
Size: 617 531 798 2106 2219
Fragments arranged by size:
2219 2106 798 617 531
BsaAI yAC'GTr
Cuts at: 493 1204 2280 3346 4062 493
Size: 711 1076 1066 716 2702
Fragments arranged by size:
2702 1076 1066 716 711
BsaBI GATnn'nnATC
Cuts at: 2298 6265 2298
Size: 3967 2304
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2183 2325 3060 3069
Size: 53 83 186 936 650 142 735 9
Cuts at: 3069 4078 4460 5095 275
Size: 1009 382 635 1451
Fragments arranged by size:
1451 1009 936 735 650 635 382 186
142 83 53 9
BsaWI w'CCGG_w
Cuts at: 4704 5102 5376 5523 5934 4704
Size: 398 274 147 411 5041
Fragments arranged by size:
5041 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2294 2324 3492 3522 1178
Size: 30 165 30 891 30 1168 30 3927
Fragments arranged by size:
3927 1168 891 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2062 1186
Size: 876 5395
BseYI C'CCAG_C
Cuts at: 2077 2111 2206 3364 5474 5970 2077
Size: 34 95 1158 2110 496 2378
Fragments arranged by size:
2378 2110 1158 496 95 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2336 2630 2693 3293 1163
Size: 573 600 294 63 600 4141
Fragments arranged by size:
4141 600 600 573 294 63
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3135 3721 4482 5510 1540
Size: 275 186 1134 586 761 1028 2301
Fragments arranged by size:
2301 1134 1028 761 586 275 186
BsiHKAI G_wGCw'C
Cuts at: 2658 3258 3342 4332 4417 5488 2658
Size: 600 84 990 85 1071 3441
Fragments arranged by size:
3441 1071 990 600 85 84
BsmI GAATG_Cn'
Cuts at: 3566 3782 3875 3566
Size: 216 93 5962
Fragments arranged by size:
5962 216 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2123 2535 2658 2823 2895 2977 3111
Size: 399 333 412 123 165 72 82 134
Cuts at: 3111 3162 3258 3299 3342 3505 3590 4332 4417
Size: 51 96 41 43 163 85 742 85
Cuts at: 4417 5488 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 412 399 333 165 163
134 123 96 85 85 82 72 51
43 41
BspEI T'CCGG_A
Cuts at: 5934 5934
Size: 6271
BspHI T'CATG_A
Cuts at: 4160 5890 4160
Size: 1730 4541
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2427 2856 3132 3545 3626 844
Size: 1583 429 276 413 81 3489
Fragments arranged by size:
3489 1583 429 413 276 81
BsrBI CCG'CTC
Cuts at: 1394 1823 4158 1394
Size: 429 2335 3507
Fragments arranged by size:
3507 2335 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4766 4940 65
Size: 1183 3518 174 1396
Fragments arranged by size:
3518 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2133 2249 2621 4915 5102 1700
Size: 110 323 116 372 2294 187 2869
Fragments arranged by size:
2869 2294 372 323 187 116 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6271
BssHII G'CGCG_C
Cuts at: 1925 2245 2606 1925
Size: 320 361 5590
Fragments arranged by size:
5590 361 320
BssSI C'ACGA_G
Cuts at: 2983 3529 4331 5343 2983
Size: 546 802 1012 3911
Fragments arranged by size:
3911 1012 802 546
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2857 794
Size: 2063 4208
BstBI TT'CG_AA
Cuts at: 5075 5075
Size: 6271
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6271
BstYI r'GATC_y
Cuts at: 1972 2898 3276 3601 3968 4353 4370 5822 5908
Size: 926 378 325 367 385 17 1452 86
Cuts at: 5908 5920 6266 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 926 385 378 367 346 325
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3374 3374
Size: 6271
BtgI C'CryG_G
Cuts at: 513 1065 2004 2476 3234 3300 513
Size: 552 939 472 758 66 3484
Fragments arranged by size:
3484 939 758 552 472 66
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5532
Fragments arranged by size:
5532 683 56
BtsI GCAGTG_nn'
Cuts at: 3032 3154 3787 4581 4601 3032
Size: 122 633 794 20 4702
Fragments arranged by size:
4702 794 633 122 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6271
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6236
DraI TTT'AAA
Cuts at: 3575 3575
Size: 6271
DraIII CAC_nnn'GTG
Cuts at: 3286 3286
Size: 6271
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5278 809
Size: 507 3962 1802
Fragments arranged by size:
3962 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2251 2739 2937
Size: 54 1425 50 275 18 421 488 198
Cuts at: 2937 3118 3132 3174 3718 4607 6236 8
Size: 181 14 42 544 889 1629 43
Fragments arranged by size:
1629 1425 889 544 488 421 275 198
181 54 50 43 42 18 14
EagI C'GGCC_G
Cuts at: 1537 1812 3132 3718 1537
Size: 275 1320 586 4090
Fragments arranged by size:
4090 1320 586 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2477 3246 4201 1353
Size: 1124 769 955 3423
Fragments arranged by size:
3423 1124 955 769
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4858 5232 5378 387
Size: 1064 138 3269 374 146 1280
Fragments arranged by size:
3269 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2798 4334 4931 5718 5986 1239
Size: 393 1166 1536 597 787 268 1524
Fragments arranged by size:
1536 1524 1166 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2177 3092 2177
Size: 915 5356
EcoO109I rG'GnC_Cy
Cuts at: 2052 2082 3158 3159 2052
Size: 30 1076 1 5164
Fragments arranged by size:
5164 1076 30 1
EcoRI G'AATT_C
Cuts at: 3580 3958 3580
Size: 378 5893
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2695 2695
Size: 6271
FspI TGC'GCA
Cuts at: 2423 2540 4777 2423
Size: 117 2237 3917
Fragments arranged by size:
3917 2237 117
FspAI rTGC'GCAy
Cuts at: 2540 2540
Size: 6271
HaeII r_GCGC'y
Cuts at: 1678 2168 2186 2459 3010 5418 1678
Size: 490 18 273 551 2408 2531
Fragments arranged by size:
2531 2408 551 490 273 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3421 3453 4912 4944 4986
Size: 32 135 32 1849 32 1459 32 42
Cuts at: 4986 5018 1373
Size: 32 2626
Fragments arranged by size:
2626 1849 1459 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3615 3861 669
Size: 988 281 1677 246 3079
Fragments arranged by size:
3079 1677 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6271
HpaI GTT'AAC
Cuts at: 3861 3861
Size: 6271
KasI G'GCGC_C
Cuts at: 2182 2182
Size: 6271
KpnI G_GTAC'C
Cuts at: 3596 3596
Size: 6271
MfeI C'AATT_G
Cuts at: 3870 3870
Size: 6271
MluI A'CGCG_T
Cuts at: 6147 6147
Size: 6271
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2394 2895 5385 5569 1305
Size: 1089 501 2490 184 2007
Fragments arranged by size:
2490 2007 1089 501 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2741 10
Size: 54 1425 343 909 3540
Fragments arranged by size:
3540 1425 909 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4229 4588 4747 518
Size: 648 3063 359 159 2042
Fragments arranged by size:
3063 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2115 2128 2863 3117 3687 4364 5512 5757
Size: 509 13 735 254 570 677 1148 245
Cuts at: 5757 1606
Size: 2120
Fragments arranged by size:
2120 1148 735 677 570 509 254 245
13
NaeI GCC'GGC
Cuts at: 2251 2623 2251
Size: 372 5899
NarI GG'CG_CC
Cuts at: 2183 2183
Size: 6271
NcoI C'CATG_G
Cuts at: 513 1065 2004 3300 513
Size: 552 939 1296 3484
Fragments arranged by size:
3484 1296 939 552
NdeI CA'TA_TG
Cuts at: 387 2505 387
Size: 2118 4153
NgoMIV G'CCGG_C
Cuts at: 2249 2621 2249
Size: 372 5899
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6271
NotI GC'GGCC_GC
Cuts at: 3718 3718
Size: 6271
NsiI A_TGCA'T
Cuts at: 3438 3438
Size: 6271
NspI r_CATG'y
Cuts at: 1132 1229 3629 5174 1132
Size: 97 2400 1545 2229
Fragments arranged by size:
2400 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6271
PciI A'CATG_T
Cuts at: 5170 5170
Size: 6271
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3286 1108
Size: 2178 4093
PfoI T'CCnGG_A
Cuts at: 2547 3063 3350 2547
Size: 516 287 5468
Fragments arranged by size:
5468 516 287
PmeI GTTT'AAAC
Cuts at: 3575 3575
Size: 6271
PmlI CAC'GTG
Cuts at: 2280 2280
Size: 6271
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4340 4372 5853 5885 4340
Size: 32 1481 32 4726
Fragments arranged by size:
4726 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6271
PsiI TTA'TAA
Cuts at: 3841 3841
Size: 6271
PspOMI G'GGCC_C
Cuts at: 3158 3158
Size: 6271
PspXI vC'TCGA_Gb
Cuts at: 2651 2651
Size: 6271
PstI C_TGCA'G
Cuts at: 830 2454 2853 2862 3623 830
Size: 1624 399 9 761 3478
Fragments arranged by size:
3478 1624 761 399 9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6271
PvuII CAG'CTG
Cuts at: 2128 2863 2128
Size: 735 5536
SalI G'TCGA_C
Cuts at: 1936 3613 1936
Size: 1677 4594
SapI GCTCTTCn'nnn_
Cuts at: 3246 3246
Size: 6271
SbfI CC_TGCA'GG
Cuts at: 3623 3623
Size: 6271
ScaI AGT'ACT
Cuts at: 4519 6231 4519
Size: 1712 4559
SfcI C'TryA_G
Cuts at: 826 1045 2450 2849 2858 3619 4754 5435 5626
Size: 219 1405 399 9 761 1135 681 191
Cuts at: 5626 826
Size: 1471
Fragments arranged by size:
1471 1405 1135 761 681 399 219 191
9
SfoI GGC'GCC
Cuts at: 2184 2184
Size: 6271
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6271
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6271
SmaI CCC'GGG
Cuts at: 1872 3598 1872
Size: 1726 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2564 2594 2651 4375 5276 5538
Size: 197 926 621 30 57 1724 901 262
Cuts at: 5538 820
Size: 1553
Fragments arranged by size:
1724 1553 926 901 621 262 197 57
30
SnaBI TAC'GTA
Cuts at: 493 4062 493
Size: 3569 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6271
SphI G_CATG'C
Cuts at: 1132 3629 1132
Size: 2497 3774
SspI AAT'ATT
Cuts at: 5 52 4195 5
Size: 47 4143 2081
Fragments arranged by size:
4143 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6271
StyI C'CwwG_G
Cuts at: 513 1065 2004 3300 3710 513
Size: 552 939 1296 410 3074
Fragments arranged by size:
3074 1296 939 552 410
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2012 2396 4298 4315 4468 4653 5098
Size: 1255 432 384 1902 17 153 185 445
Cuts at: 5098 325
Size: 1498
Fragments arranged by size:
1902 1498 1255 445 432 384 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2154 2811 4517
Size: 274 80 33 51 1443 177 657 1706
Cuts at: 4517 6229 96
Size: 1712 138
Fragments arranged by size:
1712 1706 1443 657 274 177 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2180 2212 2294 2326 2837 2869 3492 3524 6231
Size: 32 82 32 511 32 623 32 2707
Cuts at: 6231 6263 2180
Size: 32 2188
Fragments arranged by size:
2707 2188 623 511 82 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2666 1316
Size: 1350 4921
XbaI T'CTAG_A
Cuts at: 3607 3607
Size: 6271
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3439 3439
Size: 6271
XhoI C'TCGA_G
Cuts at: 1943 2651 1943
Size: 708 5563
XmaI C'CCGG_G
Cuts at: 1870 3596 1870
Size: 1726 4545
XmnI GAAnn'nnTTC
Cuts at: 3359 4400 3359
Size: 1041 5230
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3061 4079 276
Size: 53 83 186 936 1527 1018 2468
Fragments arranged by size:
2468 1527 1018 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BsiWI BsmBI BstXI
BstZ17I EcoICRI EcoRV FseI NruI PacI PpuMI PsrI
RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI