Restriction map for
pFN21AB8841
(Circular) (Six-base) MAPSORT of: pFN21AB8841.seq Check: 1511 from: 1 to: 6130
pFN21AB8841 6130 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3940 278
Size: 53 83 186 936 2404 2468
Fragments arranged by size:
2468 2404 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3473 1937
Size: 1536 4594
Acc65I G'GTAC_C
Cuts at: 3451 3451
Size: 6130
AclI AA'CG_TT
Cuts at: 1563 4257 4630 1563
Size: 2694 373 3063
Fragments arranged by size:
3063 2694 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2108 2652 2719 2838 4193 5577 5845 1632
Size: 476 544 67 119 1355 1384 268 1917
Fragments arranged by size:
1917 1384 1355 544 476 268 119 67
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5933
AflIII A'CryG_T
Cuts at: 1203 5029 6006 1203
Size: 3826 977 1327
Fragments arranged by size:
3826 1327 977
AgeI A'CCGG_T
Cuts at: 4961 4961
Size: 6130
AhdI GACnn_n'nnGTC
Cuts at: 4859 4859
Size: 6130
AleI CACnn'nnGTG
Cuts at: 1166 3297 1166
Size: 2131 3999
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3301 1184
Size: 2117 4013
AlwNI CAG_nnn'CTG
Cuts at: 2080 3400 3873 5445 2080
Size: 1320 473 1572 2765
Fragments arranged by size:
2765 1572 1320 473
ApaLI G'TGCA_C
Cuts at: 4187 5343 4187
Size: 1156 4974
ApoI r'AATT_y
Cuts at: 1493 2055 2467 2600 3439 3665 3817 5926 1493
Size: 562 412 133 839 226 152 2109 1697
Fragments arranged by size:
2109 1697 839 562 412 226 152 133
AseI AT'TA_AT
Cuts at: 160 1032 4684 160
Size: 872 3652 1606
Fragments arranged by size:
3652 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6130
AvaI C'yCGr_G
Cuts at: 1870 1943 2364 3239 3455 1870
Size: 73 421 875 216 4545
Fragments arranged by size:
4545 875 421 216 73
BamHI G'GATC_C
Cuts at: 2482 3078 3460 3827 2482
Size: 596 382 367 4785
Fragments arranged by size:
4785 596 382 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2512 2802 3451 4906 618
Size: 325 217 627 725 290 649 1455 1842
Fragments arranged by size:
1842 1455 725 649 627 325 290 217
BanII G_rGCy'C
Cuts at: 1391 2187 2463 3449 1391
Size: 796 276 986 4072
Fragments arranged by size:
4072 986 796 276
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5159
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4321 4355 1903
Size: 34 2384 34 3678
Fragments arranged by size:
3678 2384 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2820 2938 4022 5232 2820
Size: 118 1084 1210 3718
Fragments arranged by size:
3718 1210 1084 118
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6130
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2228 3404 4741
Size: 107 122 71 631 605 556 1176 1337
Cuts at: 4741 136
Size: 1525
Fragments arranged by size:
1525 1337 1176 631 605 556 122 107
71
BglII A'GATC_T
Cuts at: 6125 6125
Size: 6130
BlpI GC'TnA_GC
Cuts at: 3547 3547
Size: 6130
Bme1580I G_kGCm'C
Cuts at: 1790 2805 4191 5347 1790
Size: 1015 1386 1156 2573
Fragments arranged by size:
2573 1386 1156 1015
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6130
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3067 3861 4819
Size: 416 53 83 399 24 1650 794 958
Cuts at: 4819 442
Size: 1753
Fragments arranged by size:
1753 1650 958 794 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6130
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2087 2303 2340 2600 2654 2924 2930 4790
Size: 848 216 37 260 54 270 6 1860
Cuts at: 4790 1239
Size: 2579
Fragments arranged by size:
2579 1860 848 270 260 216 54 37
6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2052 2573 3030 4255 5120 5418 2052
Size: 521 457 1225 865 298 2764
Fragments arranged by size:
2764 1225 865 521 457 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4793 882
Size: 617 3294 2219
Fragments arranged by size:
3294 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3921 493
Size: 711 2717 2702
Fragments arranged by size:
2717 2702 711
BsaBI GATnn'nnATC
Cuts at: 3198 6124 3198
Size: 2926 3204
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2351 3937 4319 4954
Size: 53 83 186 936 818 1586 382 635
Cuts at: 4954 275
Size: 1451
Fragments arranged by size:
1586 1451 936 818 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4563 4961 5235 5382 5793 4563
Size: 398 274 147 411 4900
Fragments arranged by size:
4900 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2922 2952 1178
Size: 30 165 30 1519 30 4356
Fragments arranged by size:
4356 1519 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6130
BseYI C'CCAG_C
Cuts at: 2215 2302 2329 3136 5333 5829 2215
Size: 87 27 807 2197 496 2516
Fragments arranged by size:
2516 2197 807 496 87 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2093 1163
Size: 573 357 5200
Fragments arranged by size:
5200 573 357
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3580 4341 5369 1540
Size: 275 186 1579 761 1028 2301
Fragments arranged by size:
2301 1579 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2205 2940 4191 4276 5347 2205
Size: 735 1251 85 1071 2988
Fragments arranged by size:
2988 1251 1071 735 85
BsmI GAATG_Cn'
Cuts at: 3641 3734 3641
Size: 93 6037
BsmBI CGTCTCn'nnnn_
Cuts at: 2358 2358
Size: 6130
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2187 2205 2463 2805 2940 3449 4191
Size: 399 397 18 258 342 135 509 742
Cuts at: 4191 4276 5347 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 509 399 397 342 258
135 85 18
BspEI T'CCGG_A
Cuts at: 5793 5793
Size: 6130
BspHI T'CATG_A
Cuts at: 4019 5749 4019
Size: 1730 4400
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3485 844
Size: 2641 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4017 1394
Size: 429 2194 3507
Fragments arranged by size:
3507 2194 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2921 3039 4625 4799 65
Size: 1183 1673 118 1586 174 1396
Fragments arranged by size:
1673 1586 1396 1183 174 118
BsrFI r'CCGG_y
Cuts at: 1700 1810 4774 4961 1700
Size: 110 2964 187 2869
Fragments arranged by size:
2964 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6130
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6130
BssSI C'ACGA_G
Cuts at: 4190 5202 4190
Size: 1012 5118
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6130
BstBI TT'CG_AA
Cuts at: 4934 4934
Size: 6130
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6130
BstXI CCAn_nnnn'nTGG
Cuts at: 2223 2310 2223
Size: 87 6043
BstYI r'GATC_y
Cuts at: 1972 2112 2281 2397 2482 3078 3460 3827 4212
Size: 140 169 116 85 596 382 367 385
Cuts at: 4212 4229 5681 5767 5779 6125 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 596 385 382 367 346 169
140 116 86 85 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4639
Fragments arranged by size:
4639 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5391
Fragments arranged by size:
5391 683 56
BtsI GCAGTG_nn'
Cuts at: 2081 3646 4440 4460 2081
Size: 1565 794 20 3751
Fragments arranged by size:
3751 1565 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6130
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2057 2092 529
Size: 35 1493 35 4567
Fragments arranged by size:
4567 1493 35 35
DraI TTT'AAA
Cuts at: 3434 3434
Size: 6130
DraIII CAC_nnn'GTG
Cuts at: 3168 3168
Size: 6130
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3243 5137 809
Size: 507 1927 1894 1802
Fragments arranged by size:
1927 1894 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3577 4466 6095
Size: 54 1425 50 275 18 1747 889 1629
Cuts at: 6095 8
Size: 43
Fragments arranged by size:
1747 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3577 1537
Size: 275 1765 4090
Fragments arranged by size:
4090 1765 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3177 4060 1353
Size: 1824 883 3423
Fragments arranged by size:
3423 1824 883
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4717 5091 5237 387
Size: 1064 138 3128 374 146 1280
Fragments arranged by size:
3128 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2087 2108 2303 2340 2600 2652 2654
Size: 393 455 21 195 37 260 52 2
Cuts at: 2654 2719 2838 2924 2930 4193 4790 5577 5845
Size: 65 119 86 6 1263 597 787 268
Cuts at: 5845 1239
Size: 1524
Fragments arranged by size:
1524 1263 787 597 455 393 268 260
195 119 86 65 52 37 21 6
2
EcoO109I rG'GnC_Cy
Cuts at: 2326 2423 2683 3397 2326
Size: 97 260 714 5059
Fragments arranged by size:
5059 714 260 97
EcoRI G'AATT_C
Cuts at: 2467 2600 3439 3817 2467
Size: 133 839 378 4780
Fragments arranged by size:
4780 839 378 133
EcoRV GAT'ATC
Cuts at: 2893 2893
Size: 6130
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3113 3113
Size: 6130
FspI TGC'GCA
Cuts at: 4636 4636
Size: 6130
HaeII r_GCGC'y
Cuts at: 1678 5277 1678
Size: 3599 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3107 3139 4771 4803 4845
Size: 32 135 32 1535 32 1632 32 42
Cuts at: 4845 4877 1373
Size: 32 2626
Fragments arranged by size:
2626 1632 1535 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3474 3720 669
Size: 988 281 1536 246 3079
Fragments arranged by size:
3079 1536 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2753 748
Size: 2005 4125
HpaI GTT'AAC
Cuts at: 3720 3720
Size: 6130
KpnI G_GTAC'C
Cuts at: 3455 3455
Size: 6130
MfeI C'AATT_G
Cuts at: 2845 3729 2845
Size: 884 5246
MluI A'CGCG_T
Cuts at: 6006 6006
Size: 6130
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5244 5428 1305
Size: 3939 184 2007
Fragments arranged by size:
3939 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4308
Fragments arranged by size:
4308 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2933 3297 4088 4447 4606 518
Size: 648 1767 364 791 359 159 2042
Fragments arranged by size:
2042 1767 791 648 364 359 159
MspA1I CmG'CkG
Cuts at: 1606 2776 3254 3546 4223 5371 5616 1606
Size: 1170 478 292 677 1148 245 2120
Fragments arranged by size:
2120 1170 1148 677 478 292 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4639
Fragments arranged by size:
4639 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6130
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6130
NotI GC'GGCC_GC
Cuts at: 3577 3577
Size: 6130
NspI r_CATG'y
Cuts at: 1132 1229 3488 5033 1132
Size: 97 2259 1545 2229
Fragments arranged by size:
2259 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2292 1348
Size: 944 5186
PciI A'CATG_T
Cuts at: 5029 5029
Size: 6130
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6130
PfoI T'CCnGG_A
Cuts at: 2319 2940 2319
Size: 621 5509
PmeI GTTT'AAAC
Cuts at: 3434 3434
Size: 6130
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4199 4231 5712 5744 4199
Size: 32 1481 32 4585
Fragments arranged by size:
4585 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6130
PsiI TTA'TAA
Cuts at: 3700 3700
Size: 6130
PstI C_TGCA'G
Cuts at: 830 2536 3226 3405 3482 830
Size: 1706 690 179 77 3478
Fragments arranged by size:
3478 1706 690 179 77
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6130
PvuII CAG'CTG
Cuts at: 2776 3254 2776
Size: 478 5652
SalI G'TCGA_C
Cuts at: 1936 3472 1936
Size: 1536 4594
SbfI CC_TGCA'GG
Cuts at: 3405 3482 3405
Size: 77 6053
ScaI AGT'ACT
Cuts at: 4378 6090 4378
Size: 1712 4418
SfcI C'TryA_G
Cuts at: 826 1045 2532 2703 3222 3401 3478 4613 5294
Size: 219 1487 171 519 179 77 1135 681
Cuts at: 5294 5485 826
Size: 191 1471
Fragments arranged by size:
1487 1471 1135 681 519 219 191 179
171 77
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6130
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6130
SmaI CCC'GGG
Cuts at: 1872 2366 3457 1872
Size: 494 1091 4545
Fragments arranged by size:
4545 1091 494
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2067 2552 3045 4234 5135 5397
Size: 197 926 124 485 493 1189 901 262
Cuts at: 5397 820
Size: 1553
Fragments arranged by size:
1553 1189 926 901 493 485 262 197
124
SnaBI TAC'GTA
Cuts at: 493 3921 493
Size: 3428 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6130
SphI G_CATG'C
Cuts at: 1132 3488 1132
Size: 2356 3774
SspI AAT'ATT
Cuts at: 5 52 2494 4054 5
Size: 47 2442 1560 2081
Fragments arranged by size:
2442 2081 1560 47
StuI AGG'CCT
Cuts at: 1517 3422 1517
Size: 1905 4225
StyI C'CwwG_G
Cuts at: 513 1065 2004 2142 2178 3004 3569 513
Size: 552 939 138 36 826 565 3074
Fragments arranged by size:
3074 939 826 565 552 138 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2450 4157 4174 4327 4512 4957 325
Size: 1255 870 1707 17 153 185 445 1498
Fragments arranged by size:
1707 1498 1255 870 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4376 6088 96
Size: 274 80 33 51 1443 2399 1712 138
Fragments arranged by size:
2399 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6090 6122 6090
Size: 32 6098
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6130
XbaI T'CTAG_A
Cuts at: 3466 3466
Size: 6130
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6130
XmaI C'CCGG_G
Cuts at: 1870 2364 3455 1870
Size: 494 1091 4545
Fragments arranged by size:
4545 1091 494
XmnI GAAnn'nnTTC
Cuts at: 4259 4259
Size: 6130
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3938 276
Size: 53 83 186 936 2404 2468
Fragments arranged by size:
2468 2404 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI
EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI