Restriction map for
pFN21AB8838
(Circular) (Six-base) MAPSORT of: pFN21AB8838.seq Check: 1678 from: 1 to: 6094
pFN21AB8838 6094 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2323 2323
Size: 6094
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3135 3904 278
Size: 53 83 186 936 1599 769 2468
Fragments arranged by size:
2468 1599 936 769 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3437 1937
Size: 1500 4594
Acc65I G'GTAC_C
Cuts at: 3415 3415
Size: 6094
AclI AA'CG_TT
Cuts at: 1563 4221 4594 1563
Size: 2658 373 3063
Fragments arranged by size:
3063 2658 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2189 4157 5541 5809 1632
Size: 557 1968 1384 268 1917
Fragments arranged by size:
1968 1917 1384 557 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5897
AflIII A'CryG_T
Cuts at: 1203 4993 5970 1203
Size: 3790 977 1327
Fragments arranged by size:
3790 1327 977
AgeI A'CCGG_T
Cuts at: 3104 4925 3104
Size: 1821 4273
AhdI GACnn_n'nnGTC
Cuts at: 2380 4823 2380
Size: 2443 3651
AleI CACnn'nnGTG
Cuts at: 1166 3059 1166
Size: 1893 4201
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3077 1184
Size: 1893 4201
AlwNI CAG_nnn'CTG
Cuts at: 2251 2320 2833 3231 3837 5409 2251
Size: 69 513 398 606 1572 2936
Fragments arranged by size:
2936 1572 606 513 398 69
ApaI G_GGCC'C
Cuts at: 2198 2806 3142 2198
Size: 608 336 5150
Fragments arranged by size:
5150 608 336
ApaLI G'TGCA_C
Cuts at: 4151 5307 4151
Size: 1156 4938
ApoI r'AATT_y
Cuts at: 1493 3146 3403 3629 3781 5890 1493
Size: 1653 257 226 152 2109 1697
Fragments arranged by size:
2109 1697 1653 257 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4648 160
Size: 872 3616 1606
Fragments arranged by size:
3616 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6094
AvaI C'yCGr_G
Cuts at: 1870 1943 2255 3042 3419 1870
Size: 73 312 787 377 4545
Fragments arranged by size:
4545 787 377 312 73
BamHI G'GATC_C
Cuts at: 2551 3424 3791 2551
Size: 873 367 4854
Fragments arranged by size:
4854 873 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2060 2345 3101 3415 4870
Size: 325 217 627 273 285 756 314 1455
Cuts at: 4870 618
Size: 1842
Fragments arranged by size:
1842 1455 756 627 325 314 285 273
217
BanII G_rGCy'C
Cuts at: 1391 2072 2198 2806 2978 3116 3142 3413 1391
Size: 681 126 608 172 138 26 271 4072
Fragments arranged by size:
4072 681 608 271 172 138 126 26
BbeI G_GCGC'C
Cuts at: 2064 2064
Size: 6094
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5123
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4285 4319 1903
Size: 34 2348 34 3678
Fragments arranged by size:
3678 2348 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3986 5196 3986
Size: 1210 4884
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6094
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2432 4705 136
Size: 107 122 71 631 605 760 2273 1525
Fragments arranged by size:
2273 1525 760 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6089 6089
Size: 6094
BlpI GC'TnA_GC
Cuts at: 3071 3511 3071
Size: 440 5654
Bme1580I G_kGCm'C
Cuts at: 1790 2198 2291 2806 3104 3142 4155 5311 1790
Size: 408 93 515 298 38 1013 1156 2573
Fragments arranged by size:
2573 1156 1013 515 408 298 93 38
BmgBI CAC'GTC
Cuts at: 1318 2377 2989 1318
Size: 1059 612 4423
Fragments arranged by size:
4423 1059 612
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2778 2843 3825
Size: 416 53 83 399 24 1361 65 982
Cuts at: 3825 4783 442
Size: 958 1753
Fragments arranged by size:
1753 1361 982 958 416 399 83 65
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6094
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3251 3251
Size: 6094
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2192 2300 2311 3173 3197 3409 4754 1239
Size: 953 108 11 862 24 212 1345 2579
Fragments arranged by size:
2579 1345 953 862 212 108 24 11
Bpu10I CC'TnA_GC
Cuts at: 3209 3209
Size: 6094
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2105 4219 5084 5382 2105
Size: 2114 865 298 2817
Fragments arranged by size:
2817 2114 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2723 4757 882
Size: 617 1224 2034 2219
Fragments arranged by size:
2219 2034 1224 617
BsaAI yAC'GTr
Cuts at: 493 1204 3885 493
Size: 711 2681 2702
Fragments arranged by size:
2702 2681 711
BsaBI GATnn'nnATC
Cuts at: 6088 6088
Size: 6094
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2061 3132 3901 4283
Size: 53 83 186 936 528 1071 769 382
Cuts at: 4283 4918 275
Size: 635 1451
Fragments arranged by size:
1451 1071 936 769 635 528 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3104 4527 4925 5199 5346 5757 3104
Size: 1423 398 274 147 411 3441
Fragments arranged by size:
3441 1423 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5869
Fragments arranged by size:
5869 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6094
BseYI C'CCAG_C
Cuts at: 2010 2284 3341 5297 5793 2010
Size: 274 1057 1956 496 2311
Fragments arranged by size:
2311 1956 1057 496 274
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5521
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2052 2138 2572 3068 3544 4305
Size: 275 186 51 86 434 496 476 761
Cuts at: 4305 5333 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 761 496 476 434 275 186
86 51
BsiHKAI G_wGCw'C
Cuts at: 2072 4155 4240 5311 2072
Size: 2083 85 1071 2855
Fragments arranged by size:
2855 2083 1071 85
BsmI GAATG_Cn'
Cuts at: 2400 3605 3698 2400
Size: 1205 93 4796
Fragments arranged by size:
4796 1205 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2995 2995
Size: 6094
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2072 2198 2291 2806 2978 3104 3116
Size: 399 282 126 93 515 172 126 12
Cuts at: 3116 3142 3413 4155 4240 5311 1391
Size: 26 271 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 515 399 282 271 172
126 126 93 85 26 12
BspEI T'CCGG_A
Cuts at: 5757 5757
Size: 6094
BspHI T'CATG_A
Cuts at: 3983 5713 3983
Size: 1730 4364
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2323 3202 3449 844
Size: 1479 879 247 3489
Fragments arranged by size:
3489 1479 879 247
BsrBI CCG'CTC
Cuts at: 1394 1823 2634 2840 3981 1394
Size: 429 811 206 1141 3507
Fragments arranged by size:
3507 1141 811 429 206
BsrDI GCAATG_nn'
Cuts at: 65 1248 4589 4763 65
Size: 1183 3341 174 1396
Fragments arranged by size:
3341 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2137 2501 3104 4738 4925 1700
Size: 110 327 364 603 1634 187 2869
Fragments arranged by size:
2869 1634 603 364 327 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6094
BssHII G'CGCG_C
Cuts at: 1925 2396 1925
Size: 471 5623
BssSI C'ACGA_G
Cuts at: 4154 5166 4154
Size: 1012 5082
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6094
BstBI TT'CG_AA
Cuts at: 4898 4898
Size: 6094
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6094
BstXI CCAn_nnnn'nTGG
Cuts at: 2335 2524 2335
Size: 189 5905
BstYI r'GATC_y
Cuts at: 1972 2551 2592 3001 3424 3791 4176 4193 5645
Size: 579 41 409 423 367 385 17 1452
Cuts at: 5645 5731 5743 6089 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 579 423 409 385 367 346
86 41 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2046 2575 2970 3367 513
Size: 552 939 42 529 395 397 3240
Fragments arranged by size:
3240 939 552 529 397 395 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2377 487
Size: 683 56 1151 4204
Fragments arranged by size:
4204 1151 683 56
BtsI GCAGTG_nn'
Cuts at: 3304 3610 4404 4424 3304
Size: 306 794 20 4974
Fragments arranged by size:
4974 794 306 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6094
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6059
DraI TTT'AAA
Cuts at: 3398 3398
Size: 6094
DraIII CAC_nnn'GTG
Cuts at: 2352 2352
Size: 6094
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5101 809
Size: 507 3785 1802
Fragments arranged by size:
3785 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2135 2496 2526
Size: 54 1425 50 275 18 305 361 30
Cuts at: 2526 2578 3541 4430 6059 8
Size: 52 963 889 1629 43
Fragments arranged by size:
1629 1425 963 889 361 305 275 54
52 50 43 30 18
EagI C'GGCC_G
Cuts at: 1537 1812 2135 3541 1537
Size: 275 323 1406 4090
Fragments arranged by size:
4090 1406 323 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3128 3190 4024 1353
Size: 1775 62 834 3423
Fragments arranged by size:
3423 1775 834 62
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2680 3157 4681 5055 5201 387
Size: 1064 138 1091 477 1524 374 146 1280
Fragments arranged by size:
1524 1280 1091 1064 477 374 146 138
EcoICRI GAG'CTC
Cuts at: 2070 2070
Size: 6094
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2189 2192 2300 2311 3173 3197 3409
Size: 393 557 3 108 11 862 24 212
Cuts at: 3409 4157 4754 5541 5809 1239
Size: 748 597 787 268 1524
Fragments arranged by size:
1524 862 787 748 597 557 393 268
212 108 24 11 3
EcoO109I rG'GnC_Cy
Cuts at: 2194 2195 2803 3191 3347 2194
Size: 1 608 388 156 4941
Fragments arranged by size:
4941 608 388 156 1
EcoRI G'AATT_C
Cuts at: 3403 3781 3403
Size: 378 5716
FspI TGC'GCA
Cuts at: 4600 4600
Size: 6094
HaeII r_GCGC'y
Cuts at: 1678 2045 2064 2275 2660 5241 1678
Size: 367 19 211 385 2581 2531
Fragments arranged by size:
2581 2531 385 367 211 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2366 2398 2942 2974 4735
Size: 32 135 32 794 32 544 32 1761
Cuts at: 4735 4767 4809 4841 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1761 794 544 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2296 3438 3684 669
Size: 988 281 358 1142 246 3079
Fragments arranged by size:
3079 1142 988 358 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6094
HpaI GTT'AAC
Cuts at: 3684 3684
Size: 6094
KasI G'GCGC_C
Cuts at: 2060 2060
Size: 6094
KpnI G_GTAC'C
Cuts at: 3419 3419
Size: 6094
MfeI C'AATT_G
Cuts at: 3693 3693
Size: 6094
MluI A'CGCG_T
Cuts at: 5970 5970
Size: 6094
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5208 5392 1305
Size: 3903 184 2007
Fragments arranged by size:
3903 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2498 2528 2580 10
Size: 54 1425 343 666 30 52 3524
Fragments arranged by size:
3524 1425 666 343 54 52 30
MslI CAynn'nnrTG
Cuts at: 518 1166 3059 3095 3278 4052 4411 4570 518
Size: 648 1893 36 183 774 359 159 2042
Fragments arranged by size:
2042 1893 774 648 359 183 159 36
MspA1I CmG'CkG
Cuts at: 1606 2048 2096 2113 2539 3510 4187 5335 5580
Size: 442 48 17 426 971 677 1148 245
Cuts at: 5580 1606
Size: 2120
Fragments arranged by size:
2120 1148 971 677 442 426 245 48
17
NaeI GCC'GGC
Cuts at: 2139 2139
Size: 6094
NarI GG'CG_CC
Cuts at: 2061 2061
Size: 6094
NcoI C'CATG_G
Cuts at: 513 1065 2004 2575 3367 513
Size: 552 939 571 792 3240
Fragments arranged by size:
3240 939 792 571 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6094
NgoMIV G'CCGG_C
Cuts at: 2137 2137
Size: 6094
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6094
NotI GC'GGCC_GC
Cuts at: 3541 3541
Size: 6094
NspI r_CATG'y
Cuts at: 1132 1229 3452 4997 1132
Size: 97 2223 1545 2229
Fragments arranged by size:
2229 2223 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2766 1348
Size: 1418 4676
PciI A'CATG_T
Cuts at: 4993 4993
Size: 6094
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2833 1108
Size: 1725 4369
PfoI T'CCnGG_A
Cuts at: 2922 2997 3150 2922
Size: 75 153 5866
Fragments arranged by size:
5866 153 75
PmeI GTTT'AAAC
Cuts at: 3398 3398
Size: 6094
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4163 4195 5676 5708 4163
Size: 32 1481 32 4549
Fragments arranged by size:
4549 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3191 3191
Size: 6094
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6094
PsiI TTA'TAA
Cuts at: 3664 3664
Size: 6094
PspOMI G'GGCC_C
Cuts at: 2194 2802 3138 2194
Size: 608 336 5150
Fragments arranged by size:
5150 608 336
PstI C_TGCA'G
Cuts at: 830 3361 3446 830
Size: 2531 85 3478
Fragments arranged by size:
3478 2531 85
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6094
PvuII CAG'CTG
Cuts at: 2539 2539
Size: 6094
SacI G_AGCT'C
Cuts at: 2072 2072
Size: 6094
SacII CC_GC'GG
Cuts at: 2049 2049
Size: 6094
SalI G'TCGA_C
Cuts at: 1936 3436 1936
Size: 1500 4594
SapI GCTCTTCn'nnn_
Cuts at: 3128 3128
Size: 6094
SbfI CC_TGCA'GG
Cuts at: 3361 3446 3361
Size: 85 6009
ScaI AGT'ACT
Cuts at: 4342 6054 4342
Size: 1712 4382
SfcI C'TryA_G
Cuts at: 826 1045 3357 3442 4577 5258 5449 826
Size: 219 2312 85 1135 681 191 1471
Fragments arranged by size:
2312 1471 1135 681 219 191 85
SfiI GGCCn_nnn'nGGCC
Cuts at: 2432 2432
Size: 6094
SfoI GGC'GCC
Cuts at: 2062 2062
Size: 6094
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6094
SgrAI Cr'CCGG_yG
Cuts at: 1700 2501 1700
Size: 801 5293
SmaI CCC'GGG
Cuts at: 1872 3421 1872
Size: 1549 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2120 3042 4198 5099 5361 820
Size: 197 926 177 922 1156 901 262 1553
Fragments arranged by size:
1553 1156 926 922 901 262 197 177
SnaBI TAC'GTA
Cuts at: 493 3885 493
Size: 3392 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6094
SphI G_CATG'C
Cuts at: 1132 3452 1132
Size: 2320 3774
SspI AAT'ATT
Cuts at: 5 52 4018 5
Size: 47 3966 2081
Fragments arranged by size:
3966 2081 47
StuI AGG'CCT
Cuts at: 1517 3363 3384 1517
Size: 1846 21 4227
Fragments arranged by size:
4227 1846 21
StyI C'CwwG_G
Cuts at: 513 1065 2004 2575 2847 3367 3533 513
Size: 552 939 571 272 520 166 3074
Fragments arranged by size:
3074 939 571 552 520 272 166
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3244 3317 4121 4138 4291 4476 4921
Size: 1255 1664 73 804 17 153 185 445
Cuts at: 4921 325
Size: 1498
Fragments arranged by size:
1664 1498 1255 804 445 185 153 73
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4340 6052 96
Size: 274 80 33 51 1443 2363 1712 138
Fragments arranged by size:
2363 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2543 2575 2918 2950 6054 6086 2543
Size: 32 343 32 3104 32 2551
Fragments arranged by size:
3104 2551 343 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6094
XbaI T'CTAG_A
Cuts at: 3430 3430
Size: 6094
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2216 2491 2216
Size: 275 5819
XhoI C'TCGA_G
Cuts at: 1943 3042 1943
Size: 1099 4995
XmaI C'CCGG_G
Cuts at: 1870 3419 1870
Size: 1549 4545
XmnI GAAnn'nnTTC
Cuts at: 3282 4223 3282
Size: 941 5153
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3133 3902 276
Size: 53 83 186 936 1599 769 2468
Fragments arranged by size:
2468 1599 936 769 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SacII SalI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI
BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI NruI
NsiI PacI PmlI PspXI PsrI RsrII SanDI SexAI
SrfI SwaI