Restriction map for
pFN21AB8833
(Circular) (Six-base) MAPSORT of: pFN21AB8833.seq Check: 2617 from: 1 to: 6064
pFN21AB8833 6064 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3874 278
Size: 53 83 186 936 2338 2468
Fragments arranged by size:
2468 2338 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2076 2135 3407 1937
Size: 139 59 1272 4594
Fragments arranged by size:
4594 1272 139 59
Acc65I G'GTAC_C
Cuts at: 2393 3385 2393
Size: 992 5072
AclI AA'CG_TT
Cuts at: 1563 4191 4564 1563
Size: 2628 373 3063
Fragments arranged by size:
3063 2628 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2198 2369 4127 5511 5779 1632
Size: 566 171 1758 1384 268 1917
Fragments arranged by size:
1917 1758 1384 566 268 171
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5867
AflIII A'CryG_T
Cuts at: 1203 4963 5940 1203
Size: 3760 977 1327
Fragments arranged by size:
3760 1327 977
AgeI A'CCGG_T
Cuts at: 4895 4895
Size: 6064
AhdI GACnn_n'nnGTC
Cuts at: 2131 4793 2131
Size: 2662 3402
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6064
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6064
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2644 2676 2644
Size: 32 6032
AlwNI CAG_nnn'CTG
Cuts at: 2201 3807 5379 2201
Size: 1606 1572 2886
Fragments arranged by size:
2886 1606 1572
ApaLI G'TGCA_C
Cuts at: 4121 5277 4121
Size: 1156 4908
ApoI r'AATT_y
Cuts at: 1493 3236 3373 3599 3751 5860 1493
Size: 1743 137 226 152 2109 1697
Fragments arranged by size:
2109 1743 1697 226 152 137
AseI AT'TA_AT
Cuts at: 160 1032 4618 160
Size: 872 3586 1606
Fragments arranged by size:
3586 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6064
AvaI C'yCGr_G
Cuts at: 1870 1943 2016 2255 3389 1870
Size: 73 73 239 1134 4545
Fragments arranged by size:
4545 1134 239 73 73
BamHI G'GATC_C
Cuts at: 2294 3394 3761 2294
Size: 1100 367 4597
Fragments arranged by size:
4597 1100 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2060 2393 3385 4840 618
Size: 325 217 627 273 333 992 1455 1842
Fragments arranged by size:
1842 1455 992 627 333 325 273 217
BanII G_rGCy'C
Cuts at: 1391 2545 3219 3383 1391
Size: 1154 674 164 4072
Fragments arranged by size:
4072 1154 674 164
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2130 928
Size: 971 231 4862
Fragments arranged by size:
4862 971 231
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4255 4289 1903
Size: 34 2318 34 3678
Fragments arranged by size:
3678 2318 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3956 5166 3956
Size: 1210 4854
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6064
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2442 4675 136
Size: 107 122 71 631 605 770 2233 1525
Fragments arranged by size:
2233 1525 770 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6059 6059
Size: 6064
BlpI GC'TnA_GC
Cuts at: 3481 3481
Size: 6064
Bme1580I G_kGCm'C
Cuts at: 1790 4125 5281 1790
Size: 2335 1156 2573
Fragments arranged by size:
2573 2335 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6064
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3795 4753 442
Size: 416 53 83 399 24 2378 958 1753
Fragments arranged by size:
2378 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6064
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2529 2666 3297 4724 1239
Size: 1290 137 631 1427 2579
Fragments arranged by size:
2579 1427 1290 631 137
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2845 4189 5054 5352 2845
Size: 1344 865 298 3557
Fragments arranged by size:
3557 1344 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4727 882
Size: 617 3228 2219
Fragments arranged by size:
3228 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2735 3855 493
Size: 711 1531 1120 2702
Fragments arranged by size:
2702 1531 1120 711
BsaBI GATnn'nnATC
Cuts at: 6058 6058
Size: 6064
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3871 4253 4888 275
Size: 53 83 186 936 2338 382 635 1451
Fragments arranged by size:
2338 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2565 4497 4895 5169 5316 5727 2565
Size: 1932 398 274 147 411 2902
Fragments arranged by size:
2902 1932 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2527 2557 3270 3300 1178
Size: 30 165 30 1124 30 713 30 3942
Fragments arranged by size:
3942 1124 713 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2144 1186
Size: 958 5106
BseYI C'CCAG_C
Cuts at: 2189 2924 5267 5763 2189
Size: 735 2343 496 2490
Fragments arranged by size:
2490 2343 735 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2936 1163
Size: 573 1200 4291
Fragments arranged by size:
4291 1200 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3514 4275 5303 1540
Size: 275 186 1513 761 1028 2301
Fragments arranged by size:
2301 1513 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2537 2617 4125 4210 5281 2537
Size: 80 1508 85 1071 3320
Fragments arranged by size:
3320 1508 1071 85 80
BsmI GAATG_Cn'
Cuts at: 2731 3575 3668 2731
Size: 844 93 5127
Fragments arranged by size:
5127 844 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2091 2091
Size: 6064
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2537 2545 2617 3219 3383 4125 4210
Size: 399 747 8 72 602 164 742 85
Cuts at: 4210 5281 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 747 742 602 399 164 85
72 8
BspEI T'CCGG_A
Cuts at: 2565 5727 2565
Size: 3162 2902
BspHI T'CATG_A
Cuts at: 3953 5683 3953
Size: 1730 4334
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3419 844
Size: 2575 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3951 1394
Size: 429 2128 3507
Fragments arranged by size:
3507 2128 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4559 4733 65
Size: 1183 3311 174 1396
Fragments arranged by size:
3311 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4708 4895 1700
Size: 110 2898 187 2869
Fragments arranged by size:
2898 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6064
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6064
BssSI C'ACGA_G
Cuts at: 4124 5136 4124
Size: 1012 5052
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6064
BstBI TT'CG_AA
Cuts at: 4868 4868
Size: 6064
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6064
BstYI r'GATC_y
Cuts at: 1972 2294 2477 3394 3761 4146 4163 5615 5701
Size: 322 183 917 367 385 17 1452 86
Cuts at: 5701 5713 6059 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 917 385 367 346 322 183
86 17 12
BstZ17I GTA'TAC
Cuts at: 2077 2077
Size: 6064
Bsu36I CC'TnA_GG
Cuts at: 2238 2238
Size: 6064
BtgI C'CryG_G
Cuts at: 513 1065 2004 2889 513
Size: 552 939 885 3688
Fragments arranged by size:
3688 939 885 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5325
Fragments arranged by size:
5325 683 56
BtsI GCAGTG_nn'
Cuts at: 3580 4374 4394 3580
Size: 794 20 5250
Fragments arranged by size:
5250 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6064
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6029
DraI TTT'AAA
Cuts at: 3368 3368
Size: 6064
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2891 5071 809
Size: 507 1575 2180 1802
Fragments arranged by size:
2180 1802 1575 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2034 3511 4400
Size: 54 1425 50 275 18 204 1477 889
Cuts at: 4400 6029 8
Size: 1629 43
Fragments arranged by size:
1629 1477 1425 889 275 204 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3511 1537
Size: 275 1699 4090
Fragments arranged by size:
4090 1699 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2089 3994 1353
Size: 736 1905 3423
Fragments arranged by size:
3423 1905 736
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4651 5025 5171 387
Size: 1064 138 3062 374 146 1280
Fragments arranged by size:
3062 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2198 2369 2529 2666 3297 4127 4724
Size: 393 566 171 160 137 631 830 597
Cuts at: 4724 5511 5779 1239
Size: 787 268 1524
Fragments arranged by size:
1524 830 787 631 597 566 393 268
171 160 137
EcoNI CCTnn'n_nnAGG
Cuts at: 2627 2627
Size: 6064
EcoO109I rG'GnC_Cy
Cuts at: 2332 2412 2632 2332
Size: 80 220 5764
Fragments arranged by size:
5764 220 80
EcoRI G'AATT_C
Cuts at: 3236 3373 3751 3236
Size: 137 378 5549
Fragments arranged by size:
5549 378 137
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2599 2599
Size: 6064
FspI TGC'GCA
Cuts at: 4570 4570
Size: 6064
HaeII r_GCGC'y
Cuts at: 1678 5211 1678
Size: 3533 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2117 2149 3081 3113 4705
Size: 32 135 32 545 32 932 32 1592
Cuts at: 4705 4737 4779 4811 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1592 932 545 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2940 3408 3654 669
Size: 988 281 1002 468 246 3079
Fragments arranged by size:
3079 1002 988 468 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6064
HpaI GTT'AAC
Cuts at: 2940 3654 2940
Size: 714 5350
KpnI G_GTAC'C
Cuts at: 2397 3389 2397
Size: 992 5072
MfeI C'AATT_G
Cuts at: 3663 3663
Size: 6064
MluI A'CGCG_T
Cuts at: 5940 5940
Size: 6064
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5178 5362 1305
Size: 3873 184 2007
Fragments arranged by size:
3873 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4242
Fragments arranged by size:
4242 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2951 4022 4381 4540 518
Size: 648 1785 1071 359 159 2042
Fragments arranged by size:
2042 1785 1071 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2039 2304 2912 3480 4157 5305 5550 1606
Size: 433 265 608 568 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 608 568 433 265 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2889 513
Size: 552 939 885 3688
Fragments arranged by size:
3688 939 885 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6064
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6064
NotI GC'GGCC_GC
Cuts at: 3511 3511
Size: 6064
NspI r_CATG'y
Cuts at: 1132 1229 3422 4967 1132
Size: 97 2193 1545 2229
Fragments arranged by size:
2229 2193 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6064
PciI A'CATG_T
Cuts at: 4963 4963
Size: 6064
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6064
PfoI T'CCnGG_A
Cuts at: 2643 3227 2643
Size: 584 5480
PmeI GTTT'AAAC
Cuts at: 3368 3368
Size: 6064
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4133 4165 5646 5678 4133
Size: 32 1481 32 4519
Fragments arranged by size:
4519 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2412 2632 2412
Size: 220 5844
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6064
PsiI TTA'TAA
Cuts at: 2592 3059 3634 2592
Size: 467 575 5022
Fragments arranged by size:
5022 575 467
PspXI vC'TCGA_Gb
Cuts at: 2255 2255
Size: 6064
PstI C_TGCA'G
Cuts at: 830 3416 830
Size: 2586 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6064
PvuII CAG'CTG
Cuts at: 2912 2912
Size: 6064
SalI G'TCGA_C
Cuts at: 1936 3406 1936
Size: 1470 4594
SbfI CC_TGCA'GG
Cuts at: 3416 3416
Size: 6064
ScaI AGT'ACT
Cuts at: 4312 6024 4312
Size: 1712 4352
SfcI C'TryA_G
Cuts at: 826 1045 3412 4547 5228 5419 826
Size: 219 2367 1135 681 191 1471
Fragments arranged by size:
2367 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6064
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6064
SmaI CCC'GGG
Cuts at: 1872 3391 1872
Size: 1519 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2255 2824 4168 5069 5331 820
Size: 197 926 312 569 1344 901 262 1553
Fragments arranged by size:
1553 1344 926 901 569 312 262 197
SnaBI TAC'GTA
Cuts at: 493 3855 493
Size: 3362 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6064
SphI G_CATG'C
Cuts at: 1132 3422 1132
Size: 2290 3774
SspI AAT'ATT
Cuts at: 5 52 3988 5
Size: 47 3936 2081
Fragments arranged by size:
3936 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6064
StyI C'CwwG_G
Cuts at: 513 1065 2004 2322 2328 2511 2682 2889 3503
Size: 552 939 318 6 183 171 207 614
Cuts at: 3503 513
Size: 3074
Fragments arranged by size:
3074 939 614 552 318 207 183 171
6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2732 4091 4108 4261 4446 4891 325
Size: 1255 1152 1359 17 153 185 445 1498
Fragments arranged by size:
1498 1359 1255 1152 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4310 6022 96
Size: 274 80 33 51 1443 2333 1712 138
Fragments arranged by size:
2333 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2527 2559 6024 6056 2527
Size: 32 3465 32 2535
Fragments arranged by size:
3465 2535 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6064
XbaI T'CTAG_A
Cuts at: 3400 3400
Size: 6064
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2306 2553 2737 2306
Size: 247 184 5633
Fragments arranged by size:
5633 247 184
XhoI C'TCGA_G
Cuts at: 1943 2255 1943
Size: 312 5752
XmaI C'CCGG_G
Cuts at: 1870 3389 1870
Size: 1519 4545
XmnI GAAnn'nnTTC
Cuts at: 3236 4193 3236
Size: 957 5107
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3872 276
Size: 53 83 186 936 2338 2468
Fragments arranged by size:
2468 2338 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspXI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstXI DraIII EcoICRI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI