Restriction map for
pFN21AB8831
(Circular) (Six-base) MAPSORT of: pFN21AB8831.seq Check: 7927 from: 1 to: 6040
pFN21AB8831 6040 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2306 3850 278
Size: 53 83 186 936 770 1544 2468
Fragments arranged by size:
2468 1544 936 770 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3383 1937
Size: 1446 4594
Acc65I G'GTAC_C
Cuts at: 3361 3361
Size: 6040
AclI AA'CG_TT
Cuts at: 1563 3064 4167 4540 1563
Size: 1501 1103 373 3063
Fragments arranged by size:
3063 1501 1103 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3048 4103 5487 5755 1632
Size: 1416 1055 1384 268 1917
Fragments arranged by size:
1917 1416 1384 1055 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5843
AflIII A'CryG_T
Cuts at: 1203 4939 5916 1203
Size: 3736 977 1327
Fragments arranged by size:
3736 1327 977
AgeI A'CCGG_T
Cuts at: 4871 4871
Size: 6040
AhdI GACnn_n'nnGTC
Cuts at: 4769 4769
Size: 6040
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6040
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6040
AlwNI CAG_nnn'CTG
Cuts at: 3783 5355 3783
Size: 1572 4468
ApaLI G'TGCA_C
Cuts at: 4097 5253 4097
Size: 1156 4884
ApoI r'AATT_y
Cuts at: 1493 2074 3034 3349 3575 3727 5836 1493
Size: 581 960 315 226 152 2109 1697
Fragments arranged by size:
2109 1697 960 581 315 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4594 160
Size: 872 3562 1606
Fragments arranged by size:
3562 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6040
AvaI C'yCGr_G
Cuts at: 1870 1943 3365 1870
Size: 73 1422 4545
Fragments arranged by size:
4545 1422 73
BamHI G'GATC_C
Cuts at: 3370 3737 3370
Size: 367 5673
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3361 4816 618
Size: 325 217 627 1574 1455 1842
Fragments arranged by size:
1842 1574 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2019 2145 3359 1391
Size: 628 126 1214 4072
Fragments arranged by size:
4072 1214 628 126
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2153 2228 2885 3323 928
Size: 971 254 75 657 438 3645
Fragments arranged by size:
3645 971 657 438 254 75
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4231 4265 1903
Size: 34 2294 34 3678
Fragments arranged by size:
3678 2294 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3932 5142 3932
Size: 1210 4830
BclI T'GATC_A
Cuts at: 1548 2766 1548
Size: 1218 4822
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4651 136
Size: 107 122 71 631 605 2979 1525
Fragments arranged by size:
2979 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3089 6035 3089
Size: 2946 3094
BlpI GC'TnA_GC
Cuts at: 2579 3457 2579
Size: 878 5162
Bme1580I G_kGCm'C
Cuts at: 1790 4101 5257 1790
Size: 2311 1156 2573
Fragments arranged by size:
2573 2311 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6040
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2081 3771 4729
Size: 416 53 83 399 24 664 1690 958
Cuts at: 4729 442
Size: 1753
Fragments arranged by size:
1753 1690 958 664 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6040
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2820 2944 2820
Size: 124 5916
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2886 2914 4700 1239
Size: 1647 28 1786 2579
Fragments arranged by size:
2579 1786 1647 28
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2901 4165 5030 5328 2901
Size: 1264 865 298 3613
Fragments arranged by size:
3613 1264 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4703 882
Size: 617 3204 2219
Fragments arranged by size:
3204 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3831 493
Size: 711 2627 2702
Fragments arranged by size:
2702 2627 711
BsaBI GATnn'nnATC
Cuts at: 6034 6034
Size: 6040
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2303 3847 4229 4864
Size: 53 83 186 936 770 1544 382 635
Cuts at: 4864 275
Size: 1451
Fragments arranged by size:
1544 1451 936 770 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4473 4871 5145 5292 5703 4473
Size: 398 274 147 411 4810
Fragments arranged by size:
4810 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5815
Fragments arranged by size:
5815 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6040
BseYI C'CCAG_C
Cuts at: 5243 5739 5243
Size: 496 5544
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5467
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3490 4251 5279 1540
Size: 275 186 1489 761 1028 2301
Fragments arranged by size:
2301 1489 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2019 4101 4186 5257 2019
Size: 2082 85 1071 2802
Fragments arranged by size:
2802 2082 1071 85
BsmI GAATG_Cn'
Cuts at: 3551 3644 3551
Size: 93 5947
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2019 2145 3359 4101 4186 5257 1391
Size: 399 229 126 1214 742 85 1071 2174
Fragments arranged by size:
2174 1214 1071 742 399 229 126 85
BspEI T'CCGG_A
Cuts at: 5703 5703
Size: 6040
BspHI T'CATG_A
Cuts at: 2202 3046 3929 5659 2202
Size: 844 883 1730 2583
Fragments arranged by size:
2583 1730 883 844
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2577 2905 3395 844
Size: 1733 328 490 3489
Fragments arranged by size:
3489 1733 490 328
BsrBI CCG'CTC
Cuts at: 1394 1823 3927 1394
Size: 429 2104 3507
Fragments arranged by size:
3507 2104 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2573 4535 4709 65
Size: 1183 1325 1962 174 1396
Fragments arranged by size:
1962 1396 1325 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4684 4871 1700
Size: 110 2874 187 2869
Fragments arranged by size:
2874 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6040
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6040
BssSI C'ACGA_G
Cuts at: 4100 5112 4100
Size: 1012 5028
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6040
BstBI TT'CG_AA
Cuts at: 4844 4844
Size: 6040
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6040
BstYI r'GATC_y
Cuts at: 1972 3089 3370 3737 4122 4139 5591 5677 5689
Size: 1117 281 367 385 17 1452 86 12
Cuts at: 5689 6035 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1117 385 367 346 281 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2805 2805
Size: 6040
BtgI C'CryG_G
Cuts at: 513 1065 2004 2467 513
Size: 552 939 463 4086
Fragments arranged by size:
4086 939 552 463
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5301
Fragments arranged by size:
5301 683 56
BtsI GCAGTG_nn'
Cuts at: 2116 3556 4350 4370 2116
Size: 1440 794 20 3786
Fragments arranged by size:
3786 1440 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6040
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2779 2814 2995 3030 529
Size: 35 2215 35 181 35 3539
Fragments arranged by size:
3539 2215 181 35 35 35
DraI TTT'AAA
Cuts at: 3344 3344
Size: 6040
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5047 809
Size: 507 3731 1802
Fragments arranged by size:
3731 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2609 3487 4376
Size: 54 1425 50 275 18 779 878 889
Cuts at: 4376 6005 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 878 779 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3487 1537
Size: 275 1675 4090
Fragments arranged by size:
4090 1675 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2784 2980 3970 1353
Size: 1431 196 990 3423
Fragments arranged by size:
3423 1431 990 196
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4627 5001 5147 387
Size: 1064 138 3038 374 146 1280
Fragments arranged by size:
3038 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2017 2017
Size: 6040
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2886 2914 3048 4103 4700 5487 5755
Size: 393 1254 28 134 1055 597 787 268
Cuts at: 5755 1239
Size: 1524
Fragments arranged by size:
1524 1254 1055 787 597 393 268 134
28
EcoO109I rG'GnC_Cy
Cuts at: 3001 3001
Size: 6040
EcoRI G'AATT_C
Cuts at: 2074 3349 3727 2074
Size: 1275 378 4387
Fragments arranged by size:
4387 1275 378
EcoRV GAT'ATC
Cuts at: 2266 2266
Size: 6040
FspI TGC'GCA
Cuts at: 4546 4546
Size: 6040
HaeII r_GCGC'y
Cuts at: 1678 5187 1678
Size: 3509 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2422 2454 3299 3331 4681
Size: 32 135 32 850 32 845 32 1350
Cuts at: 4681 4713 4755 4787 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1350 850 845 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3384 3630 669
Size: 988 281 1446 246 3079
Fragments arranged by size:
3079 1446 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6040
HpaI GTT'AAC
Cuts at: 3630 3630
Size: 6040
KpnI G_GTAC'C
Cuts at: 3365 3365
Size: 6040
MfeI C'AATT_G
Cuts at: 2406 3639 2406
Size: 1233 4807
MluI A'CGCG_T
Cuts at: 5916 5916
Size: 6040
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2757 3125 5154 5338 1305
Size: 1452 368 2029 184 2007
Fragments arranged by size:
2029 2007 1452 368 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2611 10
Size: 54 1425 343 779 3439
Fragments arranged by size:
3439 1425 779 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2700 3998 4357 4516 518
Size: 648 1534 1298 359 159 2042
Fragments arranged by size:
2042 1534 1298 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3456 4133 5281 5526 1606
Size: 1850 677 1148 245 2120
Fragments arranged by size:
2120 1850 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2467 513
Size: 552 939 463 4086
Fragments arranged by size:
4086 939 552 463
NdeI CA'TA_TG
Cuts at: 387 2427 3336 387
Size: 2040 909 3091
Fragments arranged by size:
3091 2040 909
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6040
NotI GC'GGCC_GC
Cuts at: 3487 3487
Size: 6040
NspI r_CATG'y
Cuts at: 1132 1229 2889 3398 4943 1132
Size: 97 1660 509 1545 2229
Fragments arranged by size:
2229 1660 1545 509 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6040
PciI A'CATG_T
Cuts at: 4939 4939
Size: 6040
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6040
PmeI GTTT'AAAC
Cuts at: 3344 3344
Size: 6040
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4109 4141 5622 5654 4109
Size: 32 1481 32 4495
Fragments arranged by size:
4495 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3001 3001
Size: 6040
PshAI GACnn'nnGTC
Cuts at: 1613 2306 1613
Size: 693 5347
PsiI TTA'TAA
Cuts at: 2031 3610 2031
Size: 1579 4461
PstI C_TGCA'G
Cuts at: 830 2711 3392 830
Size: 1881 681 3478
Fragments arranged by size:
3478 1881 681
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6040
SacI G_AGCT'C
Cuts at: 2019 2019
Size: 6040
SalI G'TCGA_C
Cuts at: 1936 3382 1936
Size: 1446 4594
SanDI GG'GwC_CC
Cuts at: 3001 3001
Size: 6040
SbfI CC_TGCA'GG
Cuts at: 3392 3392
Size: 6040
ScaI AGT'ACT
Cuts at: 4288 6000 4288
Size: 1712 4328
SfcI C'TryA_G
Cuts at: 826 1045 2707 3106 3388 4523 5204 5395 826
Size: 219 1662 399 282 1135 681 191 1471
Fragments arranged by size:
1662 1471 1135 681 399 282 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6040
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6040
SmaI CCC'GGG
Cuts at: 1872 3367 1872
Size: 1495 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2916 4144 5045 5307 820
Size: 197 926 973 1228 901 262 1553
Fragments arranged by size:
1553 1228 973 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3831 493
Size: 3338 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6040
SphI G_CATG'C
Cuts at: 1132 3398 1132
Size: 2266 3774
SspI AAT'ATT
Cuts at: 5 52 3964 5
Size: 47 3912 2081
Fragments arranged by size:
3912 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6040
StyI C'CwwG_G
Cuts at: 513 1065 2004 2467 3479 513
Size: 552 939 463 1012 3074
Fragments arranged by size:
3074 1012 939 552 463
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4067 4084 4237 4422 4867 325
Size: 1255 2487 17 153 185 445 1498
Fragments arranged by size:
2487 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4286 5998 96
Size: 274 80 33 51 1443 2309 1712 138
Fragments arranged by size:
2309 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6000 6032 6000
Size: 32 6008
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6040
XbaI T'CTAG_A
Cuts at: 3376 3376
Size: 6040
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6040
XmaI C'CCGG_G
Cuts at: 1870 3365 1870
Size: 1495 4545
XmnI GAAnn'nnTTC
Cuts at: 4169 4169
Size: 6040
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2304 3848 276
Size: 53 83 186 936 770 1544 2468
Fragments arranged by size:
2468 1544 936 770 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SacI SalI SanDI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
PvuII RsrII SacII SapI SexAI SfiI SfoI SrfI
SwaI XcmI