Restriction map for
pFN21AB8825
(Circular) (Six-base) MAPSORT of: pFN21AB8825.seq Check: 3589 from: 1 to: 5950
pFN21AB8825 5950 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2516 2666 2516
Size: 150 5800
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3760 278
Size: 53 83 186 936 2224 2468
Fragments arranged by size:
2468 2224 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3033 3293 1937
Size: 1096 260 4594
Fragments arranged by size:
4594 1096 260
Acc65I G'GTAC_C
Cuts at: 3271 3271
Size: 5950
AclI AA'CG_TT
Cuts at: 1563 4077 4450 1563
Size: 2514 373 3063
Fragments arranged by size:
3063 2514 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2197 2269 4013 5397 5665 1632
Size: 565 72 1744 1384 268 1917
Fragments arranged by size:
1917 1744 1384 565 268 72
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5753
AflIII A'CryG_T
Cuts at: 1203 4849 5826 1203
Size: 3646 977 1327
Fragments arranged by size:
3646 1327 977
AgeI A'CCGG_T
Cuts at: 4781 4781
Size: 5950
AhdI GACnn_n'nnGTC
Cuts at: 4679 4679
Size: 5950
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5950
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2804 1184
Size: 1620 4330
AlwNI CAG_nnn'CTG
Cuts at: 3693 5265 3693
Size: 1572 4378
ApaI G_GGCC'C
Cuts at: 2637 2637
Size: 5950
ApaLI G'TGCA_C
Cuts at: 4007 5163 4007
Size: 1156 4794
ApoI r'AATT_y
Cuts at: 1493 3259 3485 3637 5746 1493
Size: 1766 226 152 2109 1697
Fragments arranged by size:
2109 1766 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4504 160
Size: 872 3472 1606
Fragments arranged by size:
3472 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5950
AvaI C'yCGr_G
Cuts at: 1870 1943 3241 3275 1870
Size: 73 1298 34 4545
Fragments arranged by size:
4545 1298 73 34
BamHI G'GATC_C
Cuts at: 3280 3647 3280
Size: 367 5583
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2528 2892 3271 4726 618
Size: 325 217 627 741 364 379 1455 1842
Fragments arranged by size:
1842 1455 741 627 379 364 325 217
BanII G_rGCy'C
Cuts at: 1391 2563 2637 2679 3177 3269 1391
Size: 1172 74 42 498 92 4072
Fragments arranged by size:
4072 1172 498 92 74 42
BbeI G_GCGC'C
Cuts at: 2896 2896
Size: 5950
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2038 3186 928
Size: 971 139 1148 3692
Fragments arranged by size:
3692 1148 971 139
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4141 4175 1903
Size: 34 2204 34 3678
Fragments arranged by size:
3678 2204 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3842 5052 3842
Size: 1210 4740
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5950
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4561 136
Size: 107 122 71 631 605 2889 1525
Fragments arranged by size:
2889 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5945 5945
Size: 5950
BlpI GC'TnA_GC
Cuts at: 2381 3367 2381
Size: 986 4964
Bme1580I G_kGCm'C
Cuts at: 1790 2533 2637 4011 5167 1790
Size: 743 104 1374 1156 2573
Fragments arranged by size:
2573 1374 1156 743 104
BmgBI CAC'GTC
Cuts at: 1318 3037 1318
Size: 1719 4231
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3681 4639 442
Size: 416 53 83 399 24 2264 958 1753
Fragments arranged by size:
2264 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5950
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2266 2266
Size: 5950
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2225 3161 4610 1239
Size: 986 936 1449 2579
Fragments arranged by size:
2579 1449 986 936
Bpu10I CC'TnA_GC
Cuts at: 2399 2399
Size: 5950
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4075 4940 5238 4075
Size: 865 298 4787
Fragments arranged by size:
4787 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3215 4613 882
Size: 617 1716 1398 2219
Fragments arranged by size:
2219 1716 1398 617
BsaAI yAC'GTr
Cuts at: 493 1204 3741 493
Size: 711 2537 2702
Fragments arranged by size:
2702 2537 711
BsaBI GATnn'nnATC
Cuts at: 5944 5944
Size: 5950
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2184 2893 3757 4139
Size: 53 83 186 936 651 709 864 382
Cuts at: 4139 4774 275
Size: 635 1451
Fragments arranged by size:
1451 936 864 709 651 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4383 4781 5055 5202 5613 4383
Size: 398 274 147 411 4720
Fragments arranged by size:
4720 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3116 3146 1178
Size: 30 165 30 1713 30 3982
Fragments arranged by size:
3982 1713 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2070 2321 1186
Size: 884 251 4815
Fragments arranged by size:
4815 884 251
BseYI C'CCAG_C
Cuts at: 2341 2350 2616 5153 5649 2341
Size: 9 266 2537 496 2642
Fragments arranged by size:
2642 2537 496 266 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5377
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2302 3400 4161 5189 1540
Size: 275 186 301 1098 761 1028 2301
Fragments arranged by size:
2301 1098 1028 761 301 275 186
BsiHKAI G_wGCw'C
Cuts at: 4011 4096 5167 4011
Size: 85 1071 4794
Fragments arranged by size:
4794 1071 85
BsmI GAATG_Cn'
Cuts at: 3461 3554 3461
Size: 93 5857
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2533 2563 2637 2679 3177 3269 4011
Size: 399 743 30 74 42 498 92 742
Cuts at: 4011 4096 5167 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 743 742 498 399 92 85
74 42 30
BspEI T'CCGG_A
Cuts at: 5613 5613
Size: 5950
BspHI T'CATG_A
Cuts at: 3839 5569 3839
Size: 1730 4220
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2516 2666 3305 844
Size: 1672 150 639 3489
Fragments arranged by size:
3489 1672 639 150
BsrBI CCG'CTC
Cuts at: 1394 1823 2467 3127 3837 1394
Size: 429 644 660 710 3507
Fragments arranged by size:
3507 710 660 644 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4445 4619 65
Size: 1183 3197 174 1396
Fragments arranged by size:
3197 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2385 4594 4781 1700
Size: 110 575 2209 187 2869
Fragments arranged by size:
2869 2209 575 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5950
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5950
BssSI C'ACGA_G
Cuts at: 2251 4010 5022 2251
Size: 1759 1012 3179
Fragments arranged by size:
3179 1759 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5950
BstBI TT'CG_AA
Cuts at: 4754 4754
Size: 5950
BstEII G'GTnAC_C
Cuts at: 1184 3049 1184
Size: 1865 4085
BstXI CCAn_nnnn'nTGG
Cuts at: 2911 2911
Size: 5950
BstYI r'GATC_y
Cuts at: 1972 3280 3647 4032 4049 5501 5587 5599 5945
Size: 1308 367 385 17 1452 86 12 346
Cuts at: 5945 1972
Size: 1977
Fragments arranged by size:
1977 1452 1308 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3055 3055
Size: 5950
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4459
Fragments arranged by size:
4459 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5211
Fragments arranged by size:
5211 683 56
BtsI GCAGTG_nn'
Cuts at: 2459 2481 2749 3073 3466 4260 4280 2459
Size: 22 268 324 393 794 20 4129
Fragments arranged by size:
4129 794 393 324 268 22 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5950
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5915
DraI TTT'AAA
Cuts at: 3254 3254
Size: 5950
DraIII CAC_nnn'GTG
Cuts at: 2173 2965 2173
Size: 792 5158
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2486 4957 809
Size: 507 1170 2471 1802
Fragments arranged by size:
2471 1802 1170 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2862 3016 3039
Size: 54 1425 50 275 18 1032 154 23
Cuts at: 3039 3397 4286 5915 8
Size: 358 889 1629 43
Fragments arranged by size:
1629 1425 1032 889 358 275 154 54
50 43 23 18
EagI C'GGCC_G
Cuts at: 1537 1812 3397 1537
Size: 275 1585 4090
Fragments arranged by size:
4090 1585 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2224 2540 3880 1353
Size: 871 316 1340 3423
Fragments arranged by size:
3423 1340 871 316
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2404 4537 4911 5057 387
Size: 1064 138 815 2133 374 146 1280
Fragments arranged by size:
2133 1280 1064 815 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2197 2225 2269 3161 4013 4610 5397
Size: 393 565 28 44 892 852 597 787
Cuts at: 5397 5665 1239
Size: 268 1524
Fragments arranged by size:
1524 892 852 787 597 565 393 268
44 28
EcoO109I rG'GnC_Cy
Cuts at: 2633 2634 2633
Size: 1 5949
EcoRI G'AATT_C
Cuts at: 3259 3637 3259
Size: 378 5572
FspI TGC'GCA
Cuts at: 4456 4456
Size: 5950
HaeII r_GCGC'y
Cuts at: 1678 2737 2896 3000 5097 1678
Size: 1059 159 104 2097 2531
Fragments arranged by size:
2531 2097 1059 159 104
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2941 2973 4591 4623 4665
Size: 32 135 32 1369 32 1618 32 42
Cuts at: 4665 4697 1373
Size: 32 2626
Fragments arranged by size:
2626 1618 1369 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3294 3540 669
Size: 988 281 1356 246 3079
Fragments arranged by size:
3079 1356 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5950
HpaI GTT'AAC
Cuts at: 3540 3540
Size: 5950
KasI G'GCGC_C
Cuts at: 2892 2892
Size: 5950
KpnI G_GTAC'C
Cuts at: 3275 3275
Size: 5950
MfeI C'AATT_G
Cuts at: 3549 3549
Size: 5950
MluI A'CGCG_T
Cuts at: 5826 5826
Size: 5950
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5064 5248 1305
Size: 3759 184 2007
Fragments arranged by size:
3759 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3041 10
Size: 54 1425 343 1209 2919
Fragments arranged by size:
2919 1425 1209 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2984 3908 4267 4426 518
Size: 648 1818 924 359 159 2042
Fragments arranged by size:
2042 1818 924 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2803 2890 3091 3366 4043 5191 5436 1606
Size: 1197 87 201 275 677 1148 245 2120
Fragments arranged by size:
2120 1197 1148 677 275 245 201 87
NaeI GCC'GGC
Cuts at: 2387 2387
Size: 5950
NarI GG'CG_CC
Cuts at: 2893 2893
Size: 5950
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4459
Fragments arranged by size:
4459 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5950
NgoMIV G'CCGG_C
Cuts at: 2385 2385
Size: 5950
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5950
NotI GC'GGCC_GC
Cuts at: 3397 3397
Size: 5950
NspI r_CATG'y
Cuts at: 1132 1229 3073 3308 4853 1132
Size: 97 1844 235 1545 2229
Fragments arranged by size:
2229 1844 1545 235 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5950
PciI A'CATG_T
Cuts at: 4849 4849
Size: 5950
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2860 1108
Size: 1752 4198
PfoI T'CCnGG_A
Cuts at: 2202 3120 2202
Size: 918 5032
PmeI GTTT'AAAC
Cuts at: 3254 3254
Size: 5950
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2201 2233 4019 4051 5532 5564 2201
Size: 32 1786 32 1481 32 2587
Fragments arranged by size:
2587 1786 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5950
PsiI TTA'TAA
Cuts at: 3520 3520
Size: 5950
PspOMI G'GGCC_C
Cuts at: 2633 2633
Size: 5950
PstI C_TGCA'G
Cuts at: 830 2802 3302 830
Size: 1972 500 3478
Fragments arranged by size:
3478 1972 500
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5950
PvuII CAG'CTG
Cuts at: 2803 2803
Size: 5950
SalI G'TCGA_C
Cuts at: 1936 3292 1936
Size: 1356 4594
SapI GCTCTTCn'nnn_
Cuts at: 2224 2224
Size: 5950
SbfI CC_TGCA'GG
Cuts at: 3302 3302
Size: 5950
ScaI AGT'ACT
Cuts at: 4198 5910 4198
Size: 1712 4238
SexAI A'CCwGG_T
Cuts at: 2961 2961
Size: 5950
SfcI C'TryA_G
Cuts at: 826 1045 2323 2433 2798 3298 4433 5114 5305
Size: 219 1278 110 365 500 1135 681 191
Cuts at: 5305 826
Size: 1471
Fragments arranged by size:
1471 1278 1135 681 500 365 219 191
110
SfoI GGC'GCC
Cuts at: 2894 2894
Size: 5950
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5950
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5950
SmaI CCC'GGG
Cuts at: 1872 3277 1872
Size: 1405 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4054 4955 5217 820
Size: 197 926 2111 901 262 1553
Fragments arranged by size:
2111 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3741 493
Size: 3248 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5950
SphI G_CATG'C
Cuts at: 1132 3308 1132
Size: 2176 3774
SspI AAT'ATT
Cuts at: 5 52 3874 5
Size: 47 3822 2081
Fragments arranged by size:
3822 2081 47
StuI AGG'CCT
Cuts at: 1517 2118 3060 3233 1517
Size: 601 942 173 4234
Fragments arranged by size:
4234 942 601 173
StyI C'CwwG_G
Cuts at: 513 1065 2004 3389 513
Size: 552 939 1385 3074
Fragments arranged by size:
3074 1385 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2630 2762 3037 3977 3994 4147 4332
Size: 1255 1050 132 275 940 17 153 185
Cuts at: 4332 4777 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1050 940 445 275 185 153
132 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4196 5908 96
Size: 274 80 33 51 1443 2219 1712 138
Fragments arranged by size:
2219 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3116 3148 5910 5942 3116
Size: 32 2762 32 3124
Fragments arranged by size:
3124 2762 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5950
XbaI T'CTAG_A
Cuts at: 3286 3286
Size: 5950
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2498 2911 2498
Size: 413 5537
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5950
XmaI C'CCGG_G
Cuts at: 1870 3275 1870
Size: 1405 4545
XmnI GAAnn'nnTTC
Cuts at: 4079 4079
Size: 5950
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3758 276
Size: 53 83 186 936 2224 2468
Fragments arranged by size:
2468 2224 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI
BsmBI BstZ17I EcoICRI EcoNI EcoRV FalI FseI FspAI
NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII
SacI SacII SanDI SfiI SrfI SwaI