Restriction map for
pFN21AB8823
(Circular) (Six-base) MAPSORT of: pFN21AB8823.seq Check: 4712 from: 1 to: 5917
pFN21AB8823 5917 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2901 2901
Size: 5917
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3727 278
Size: 53 83 186 936 2191 2468
Fragments arranged by size:
2468 2191 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2451 3260 1937
Size: 514 809 4594
Fragments arranged by size:
4594 809 514
Acc65I G'GTAC_C
Cuts at: 3238 3238
Size: 5917
AclI AA'CG_TT
Cuts at: 1563 4044 4417 1563
Size: 2481 373 3063
Fragments arranged by size:
3063 2481 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3980 5364 5632 1632
Size: 2348 1384 268 1917
Fragments arranged by size:
2348 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5720
AflIII A'CryG_T
Cuts at: 1203 2412 4816 5793 1203
Size: 1209 2404 977 1327
Fragments arranged by size:
2404 1327 1209 977
AgeI A'CCGG_T
Cuts at: 4748 4748
Size: 5917
AhdI GACnn_n'nnGTC
Cuts at: 4646 4646
Size: 5917
AleI CACnn'nnGTG
Cuts at: 1166 2054 1166
Size: 888 5029
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5917
AlwNI CAG_nnn'CTG
Cuts at: 2643 3660 5232 2643
Size: 1017 1572 3328
Fragments arranged by size:
3328 1572 1017
ApaI G_GGCC'C
Cuts at: 2597 2650 2597
Size: 53 5864
ApaLI G'TGCA_C
Cuts at: 3974 5130 3974
Size: 1156 4761
ApoI r'AATT_y
Cuts at: 1493 3226 3452 3604 5713 1493
Size: 1733 226 152 2109 1697
Fragments arranged by size:
2109 1733 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4471 160
Size: 872 3439 1606
Fragments arranged by size:
3439 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5917
AvaI C'yCGr_G
Cuts at: 1870 1943 3242 1870
Size: 73 1299 4545
Fragments arranged by size:
4545 1299 73
BamHI G'GATC_C
Cuts at: 3247 3614 3247
Size: 367 5550
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2620 2766 3238 4693 618
Size: 325 217 627 833 146 472 1455 1842
Fragments arranged by size:
1842 1455 833 627 472 325 217 146
BanII G_rGCy'C
Cuts at: 1391 2145 2597 2604 2650 3236 1391
Size: 754 452 7 46 586 4072
Fragments arranged by size:
4072 754 586 452 46 7
BbeI G_GCGC'C
Cuts at: 2770 2770
Size: 5917
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2704 928
Size: 971 805 4141
Fragments arranged by size:
4141 971 805
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4108 4142 1903
Size: 34 2171 34 3678
Fragments arranged by size:
3678 2171 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3809 5019 3809
Size: 1210 4707
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5917
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2795 4528 136
Size: 107 122 71 631 605 1123 1733 1525
Fragments arranged by size:
1733 1525 1123 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5912 5912
Size: 5917
BlpI GC'TnA_GC
Cuts at: 2144 3334 2144
Size: 1190 4727
Bme1580I G_kGCm'C
Cuts at: 1790 2597 2650 3047 3978 5134 1790
Size: 807 53 397 931 1156 2573
Fragments arranged by size:
2573 1156 931 807 397 53
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5917
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3648 4606 442
Size: 416 53 83 399 24 2231 958 1753
Fragments arranged by size:
2231 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5917
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2357 4577 1239
Size: 1118 2220 2579
Fragments arranged by size:
2579 2220 1118
Bpu10I CC'TnA_GC
Cuts at: 2248 2598 3135 3146 2248
Size: 350 537 11 5019
Fragments arranged by size:
5019 537 350 11
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4042 4907 5205 4042
Size: 865 298 4754
Fragments arranged by size:
4754 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4580 882
Size: 617 3081 2219
Fragments arranged by size:
3081 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2137 2794 3708 493
Size: 711 933 657 914 2702
Fragments arranged by size:
2702 933 914 711 657
BsaBI GATnn'nnATC
Cuts at: 5911 5911
Size: 5917
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2767 3724 4106 4741
Size: 53 83 186 936 1234 957 382 635
Cuts at: 4741 275
Size: 1451
Fragments arranged by size:
1451 1234 957 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4350 4748 5022 5169 5580 4350
Size: 398 274 147 411 4687
Fragments arranged by size:
4687 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2912 2942 1178
Size: 30 165 30 1509 30 4153
Fragments arranged by size:
4153 1509 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3046 1186
Size: 1860 4057
BseYI C'CCAG_C
Cuts at: 2018 2022 3075 3212 5120 5616 2018
Size: 4 1053 137 1908 496 2319
Fragments arranged by size:
2319 1908 1053 496 137 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2547 2921 1163
Size: 573 811 374 4159
Fragments arranged by size:
4159 811 573 374
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2455 3367 4128 5156 1540
Size: 275 186 454 912 761 1028 2301
Fragments arranged by size:
2301 1028 912 761 454 275 186
BsiHKAI G_wGCw'C
Cuts at: 2145 2156 3978 4063 5134 2145
Size: 11 1822 85 1071 2928
Fragments arranged by size:
2928 1822 1071 85 11
BsmI GAATG_Cn'
Cuts at: 3007 3079 3428 3521 3007
Size: 72 349 93 5403
Fragments arranged by size:
5403 349 93 72
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2145 2156 2597 2604 2650 3047 3236
Size: 399 355 11 441 7 46 397 189
Cuts at: 3236 3978 4063 5134 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 441 399 397 355 189
85 46 11 7
BspEI T'CCGG_A
Cuts at: 5580 5580
Size: 5917
BspHI T'CATG_A
Cuts at: 3806 5536 3806
Size: 1730 4187
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2901 3272 844
Size: 2057 371 3489
Fragments arranged by size:
3489 2057 371
BsrBI CCG'CTC
Cuts at: 1394 1823 3804 1394
Size: 429 1981 3507
Fragments arranged by size:
3507 1981 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4412 4586 65
Size: 1183 3164 174 1396
Fragments arranged by size:
3164 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2733 2763 2991 4561 4748 1700
Size: 110 923 30 228 1570 187 2869
Fragments arranged by size:
2869 1570 923 228 187 110 30
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5917
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5917
BssSI C'ACGA_G
Cuts at: 3977 4989 3977
Size: 1012 4905
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2509 794
Size: 1715 4202
BstBI TT'CG_AA
Cuts at: 4721 4721
Size: 5917
BstEII G'GTnAC_C
Cuts at: 1184 2047 1184
Size: 863 5054
BstXI CCAn_nnnn'nTGG
Cuts at: 2164 2197 2677 2164
Size: 33 480 5404
Fragments arranged by size:
5404 480 33
BstYI r'GATC_y
Cuts at: 1972 2079 3247 3614 3999 4016 5468 5554 5566
Size: 107 1168 367 385 17 1452 86 12
Cuts at: 5566 5912 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1168 385 367 346 107 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2565 3024 2565
Size: 459 5458
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5365
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2278 487
Size: 683 56 1052 4126
Fragments arranged by size:
4126 1052 683 56
BtsI GCAGTG_nn'
Cuts at: 2061 2770 2849 2875 3433 4227 4247 2061
Size: 709 79 26 558 794 20 3731
Fragments arranged by size:
3731 794 709 558 79 26 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5917
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5882
DraI TTT'AAA
Cuts at: 3221 3221
Size: 5917
DraIII CAC_nnn'GTG
Cuts at: 2509 2509
Size: 5917
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2459 4924 809
Size: 507 1143 2465 1802
Fragments arranged by size:
2465 1802 1143 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2187 2289 2547
Size: 54 1425 50 275 18 357 102 258
Cuts at: 2547 2796 3364 4253 5882 8
Size: 249 568 889 1629 43
Fragments arranged by size:
1629 1425 889 568 357 275 258 249
102 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3364 1537
Size: 275 1552 4090
Fragments arranged by size:
4090 1552 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2114 2618 3064 3847 1353
Size: 761 504 446 783 3423
Fragments arranged by size:
3423 783 761 504 446
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4504 4878 5024 387
Size: 1064 138 2915 374 146 1280
Fragments arranged by size:
2915 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2143 2143
Size: 5917
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2357 3980 4577 5364 5632 1239
Size: 393 725 1623 597 787 268 1524
Fragments arranged by size:
1623 1524 787 725 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3023 3023
Size: 5917
EcoO109I rG'GnC_Cy
Cuts at: 2027 2539 2593 2594 2647 2859 2027
Size: 512 54 1 53 212 5085
Fragments arranged by size:
5085 512 212 54 53 1
EcoRI G'AATT_C
Cuts at: 3226 3604 3226
Size: 378 5539
FspI TGC'GCA
Cuts at: 4423 4423
Size: 5917
HaeII r_GCGC'y
Cuts at: 1678 2478 2770 5064 1678
Size: 800 292 2294 2531
Fragments arranged by size:
2531 2294 800 292
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2912 2944 4558 4590 4632
Size: 32 135 32 1340 32 1614 32 42
Cuts at: 4632 4664 1373
Size: 32 2626
Fragments arranged by size:
2626 1614 1340 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2236 2410 2452 3261 3507 669
Size: 988 281 298 174 42 809 246 3079
Fragments arranged by size:
3079 988 809 298 281 246 174 42
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5917
HpaI GTT'AAC
Cuts at: 3507 3507
Size: 5917
KasI G'GCGC_C
Cuts at: 2766 2766
Size: 5917
KpnI G_GTAC'C
Cuts at: 3242 3242
Size: 5917
MfeI C'AATT_G
Cuts at: 3516 3516
Size: 5917
MluI A'CGCG_T
Cuts at: 5793 5793
Size: 5917
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5031 5215 1305
Size: 3726 184 2007
Fragments arranged by size:
3726 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2189 2291 10
Size: 54 1425 343 357 102 3636
Fragments arranged by size:
3636 1425 357 343 102 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2054 2195 3875 4234 4393 518
Size: 648 843 45 141 1680 359 159 2042
Fragments arranged by size:
2042 1680 843 648 359 159 141 45
MspA1I CmG'CkG
Cuts at: 1606 2022 2560 2584 3333 4010 5158 5403 1606
Size: 416 538 24 749 677 1148 245 2120
Fragments arranged by size:
2120 1148 749 677 538 416 245 24
NaeI GCC'GGC
Cuts at: 2735 2765 2993 2735
Size: 30 228 5659
Fragments arranged by size:
5659 228 30
NarI GG'CG_CC
Cuts at: 2767 2767
Size: 5917
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5365
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5917
NgoMIV G'CCGG_C
Cuts at: 2733 2763 2991 2733
Size: 30 228 5659
Fragments arranged by size:
5659 228 30
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5917
NotI GC'GGCC_GC
Cuts at: 3364 3364
Size: 5917
NspI r_CATG'y
Cuts at: 1132 1229 2416 3051 3275 4820 1132
Size: 97 1187 635 224 1545 2229
Fragments arranged by size:
2229 1545 1187 635 224 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5917
PciI A'CATG_T
Cuts at: 2412 4816 2412
Size: 2404 3513
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2587 1108
Size: 1479 4438
PmeI GTTT'AAAC
Cuts at: 3221 3221
Size: 5917
PmlI CAC'GTG
Cuts at: 2137 2137
Size: 5917
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3986 4018 5499 5531 3986
Size: 32 1481 32 4372
Fragments arranged by size:
4372 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2539 2859 2539
Size: 320 5597
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5917
PsiI TTA'TAA
Cuts at: 3487 3487
Size: 5917
PspOMI G'GGCC_C
Cuts at: 2593 2646 2593
Size: 53 5864
PstI C_TGCA'G
Cuts at: 830 3121 3269 830
Size: 2291 148 3478
Fragments arranged by size:
3478 2291 148
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5917
PvuII CAG'CTG
Cuts at: 2022 2584 2022
Size: 562 5355
SacI G_AGCT'C
Cuts at: 2145 2145
Size: 5917
SalI G'TCGA_C
Cuts at: 1936 2450 3259 1936
Size: 514 809 4594
Fragments arranged by size:
4594 809 514
SanDI GG'GwC_CC
Cuts at: 2859 2859
Size: 5917
SbfI CC_TGCA'GG
Cuts at: 3269 3269
Size: 5917
ScaI AGT'ACT
Cuts at: 4165 5877 4165
Size: 1712 4205
SexAI A'CCwGG_T
Cuts at: 2883 2883
Size: 5917
SfcI C'TryA_G
Cuts at: 826 1045 3006 3117 3265 4400 5081 5272 826
Size: 219 1961 111 148 1135 681 191 1471
Fragments arranged by size:
1961 1471 1135 681 219 191 148 111
SfoI GGC'GCC
Cuts at: 2768 2768
Size: 5917
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5917
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5917
SmaI CCC'GGG
Cuts at: 1872 3244 1872
Size: 1372 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4021 4922 5184 820
Size: 197 926 2078 901 262 1553
Fragments arranged by size:
2078 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3708 493
Size: 3215 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5917
SphI G_CATG'C
Cuts at: 1132 3275 1132
Size: 2143 3774
SspI AAT'ATT
Cuts at: 5 52 3841 5
Size: 47 3789 2081
Fragments arranged by size:
3789 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5917
StyI C'CwwG_G
Cuts at: 513 1065 2071 3036 3096 3356 513
Size: 552 1006 965 60 260 3074
Fragments arranged by size:
3074 1006 965 552 260 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2108 3089 3226 3944 3961 4114 4299
Size: 1255 528 981 137 718 17 153 185
Cuts at: 4299 4744 325
Size: 445 1498
Fragments arranged by size:
1498 1255 981 718 528 445 185 153
137 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4163 5875 96
Size: 274 80 33 51 1443 2186 1712 138
Fragments arranged by size:
2186 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5877 5909 5877
Size: 32 5885
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5917
XbaI T'CTAG_A
Cuts at: 3253 3253
Size: 5917
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2299 2674 2299
Size: 375 5542
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5917
XmaI C'CCGG_G
Cuts at: 1870 3242 1870
Size: 1372 4545
XmnI GAAnn'nnTTC
Cuts at: 4046 4046
Size: 5917
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3725 276
Size: 53 83 186 936 2191 2468
Fragments arranged by size:
2468 2191 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BsmBI BstZ17I EcoRV FalI FseI FspAI NruI
NsiI PacI PfoI PspXI PsrI RsrII SacII SapI
SfiI SrfI SwaI