Restriction map for
pFN21AB8821
(Circular) (Six-base) MAPSORT of: pFN21AB8821.seq Check: 6614 from: 1 to: 5857
pFN21AB8821 5857 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2709 2709
Size: 5857
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3667 278
Size: 53 83 186 936 2131 2468
Fragments arranged by size:
2468 2131 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3200 1937
Size: 1263 4594
Acc65I G'GTAC_C
Cuts at: 3178 3178
Size: 5857
AclI AA'CG_TT
Cuts at: 1563 3984 4357 1563
Size: 2421 373 3063
Fragments arranged by size:
3063 2421 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3920 5304 5572 1632
Size: 2288 1384 268 1917
Fragments arranged by size:
2288 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5660
AflIII A'CryG_T
Cuts at: 1203 3012 4756 5733 1203
Size: 1809 1744 977 1327
Fragments arranged by size:
1809 1744 1327 977
AgeI A'CCGG_T
Cuts at: 2042 4688 2042
Size: 2646 3211
AhdI GACnn_n'nnGTC
Cuts at: 4586 4586
Size: 5857
AleI CACnn'nnGTG
Cuts at: 1166 2905 3101 1166
Size: 1739 196 3922
Fragments arranged by size:
3922 1739 196
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5857
AlwNI CAG_nnn'CTG
Cuts at: 2244 2458 2806 2902 3600 5172 2244
Size: 214 348 96 698 1572 2929
Fragments arranged by size:
2929 1572 698 348 214 96
ApaI G_GGCC'C
Cuts at: 2771 2771
Size: 5857
ApaLI G'TGCA_C
Cuts at: 3914 5070 3914
Size: 1156 4701
ApoI r'AATT_y
Cuts at: 1493 3166 3392 3544 5653 1493
Size: 1673 226 152 2109 1697
Fragments arranged by size:
2109 1697 1673 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4411 160
Size: 872 3379 1606
Fragments arranged by size:
3379 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5857
AvaI C'yCGr_G
Cuts at: 1870 1943 2276 2433 2746 3182 1870
Size: 73 333 157 313 436 4545
Fragments arranged by size:
4545 436 333 313 157 73
BamHI G'GATC_C
Cuts at: 3187 3554 3187
Size: 367 5490
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2437 2469 2542 2555 2750
Size: 325 217 627 650 32 73 13 195
Cuts at: 2750 2777 2941 3131 3178 4633 618
Size: 27 164 190 47 1455 1842
Fragments arranged by size:
1842 1455 650 627 325 217 195 190
164 73 47 32 27 13
BanII G_rGCy'C
Cuts at: 1391 2283 2307 2771 3176 1391
Size: 892 24 464 405 4072
Fragments arranged by size:
4072 892 464 405 24
BbeI G_GCGC'C
Cuts at: 2441 2473 2559 2754 2781 2441
Size: 32 86 195 27 5517
Fragments arranged by size:
5517 195 86 32 27
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2872 928
Size: 971 973 3913
Fragments arranged by size:
3913 973 971
BbvCI CC'TCA_GC
Cuts at: 2237 2237
Size: 5857
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4048 4082 1903
Size: 34 2111 34 3678
Fragments arranged by size:
3678 2111 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3749 4959 3749
Size: 1210 4647
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5857
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2600 2775 4468
Size: 107 122 71 631 605 928 175 1693
Cuts at: 4468 136
Size: 1525
Fragments arranged by size:
1693 1525 928 631 605 175 122 107
71
BglII A'GATC_T
Cuts at: 5852 5852
Size: 5857
BlpI GC'TnA_GC
Cuts at: 3274 3274
Size: 5857
Bme1580I G_kGCm'C
Cuts at: 1790 2141 2545 2771 2836 2946 3108 3134 3918
Size: 351 404 226 65 110 162 26 784
Cuts at: 3918 5074 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 784 404 351 226 162 110
65 26
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5857
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2326 3137 3163
Size: 416 53 83 399 24 909 811 26
Cuts at: 3163 3588 4546 442
Size: 425 958 1753
Fragments arranged by size:
1753 958 909 811 425 416 399 83
53 26 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5857
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4517 1239
Size: 3278 2579
Bpu10I CC'TnA_GC
Cuts at: 2237 2237
Size: 5857
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3982 4847 5145 3982
Size: 865 298 4694
Fragments arranged by size:
4694 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4520 882
Size: 617 3021 2219
Fragments arranged by size:
3021 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3015 3648 493
Size: 711 1811 633 2702
Fragments arranged by size:
2702 1811 711 633
BsaBI GATnn'nnATC
Cuts at: 5851 5851
Size: 5857
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2438 2470 2556 2751
Size: 53 83 186 936 905 32 86 195
Cuts at: 2751 2778 2842 3063 3664 4046 4681 275
Size: 27 64 221 601 382 635 1451
Fragments arranged by size:
1451 936 905 635 601 382 221 195
186 86 83 64 53 32 27
BsaWI w'CCGG_w
Cuts at: 2042 4290 4688 4962 5109 5520 2042
Size: 2248 398 274 147 411 2379
Fragments arranged by size:
2379 2248 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2003 2033 1178
Size: 30 165 30 600 30 5002
Fragments arranged by size:
5002 600 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2005 2008 2023 2090 2378 3041 1186
Size: 819 3 15 67 288 663 4002
Fragments arranged by size:
4002 819 663 288 67 15 3
BseYI C'CCAG_C
Cuts at: 2327 2362 2754 2799 5060 5556 2327
Size: 35 392 45 2261 496 2628
Fragments arranged by size:
2628 2261 496 392 45 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2615 2690 2804 2975 1163
Size: 573 879 75 114 171 4045
Fragments arranged by size:
4045 879 573 171 114 75
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3081 3307 4068 5096 1540
Size: 275 186 1080 226 761 1028 2301
Fragments arranged by size:
2301 1080 1028 761 275 226 186
BsiHKAI G_wGCw'C
Cuts at: 2277 2691 3918 4003 5074 2277
Size: 414 1227 85 1071 3060
Fragments arranged by size:
3060 1227 1071 414 85
BsmI GAATG_Cn'
Cuts at: 3368 3461 3368
Size: 93 5764
BsmBI CGTCTCn'nnnn_
Cuts at: 2849 2849
Size: 5857
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2141 2277 2283 2307 2545 2691 2771
Size: 399 351 136 6 24 238 146 80
Cuts at: 2771 2836 2946 3108 3134 3176 3918 4003 5074
Size: 65 110 162 26 42 742 85 1071
Cuts at: 5074 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 351 238 162 146
136 110 85 80 65 42 26 24
6
BspEI T'CCGG_A
Cuts at: 5520 5520
Size: 5857
BspHI T'CATG_A
Cuts at: 3746 5476 3746
Size: 1730 4127
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2430 2709 3212 844
Size: 1586 279 503 3489
Fragments arranged by size:
3489 1586 503 279
BsrBI CCG'CTC
Cuts at: 1394 1823 3039 3744 1394
Size: 429 1216 705 3507
Fragments arranged by size:
3507 1216 705 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4352 4526 65
Size: 1183 3104 174 1396
Fragments arranged by size:
3104 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2042 2091 2291 2938 4501 4688 1700
Size: 110 232 49 200 647 1563 187 2869
Fragments arranged by size:
2869 1563 647 232 200 187 110 49
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5857
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5857
BssSI C'ACGA_G
Cuts at: 2168 2402 2519 3917 4929 2168
Size: 234 117 1398 1012 3096
Fragments arranged by size:
3096 1398 1012 234 117
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2713 794
Size: 1919 3938
BstBI TT'CG_AA
Cuts at: 4661 4661
Size: 5857
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5857
BstXI CCAn_nnnn'nTGG
Cuts at: 2155 2907 2155
Size: 752 5105
BstYI r'GATC_y
Cuts at: 1972 3187 3554 3939 3956 5408 5494 5506 5852
Size: 1215 367 385 17 1452 86 12 346
Cuts at: 5852 1972
Size: 1977
Fragments arranged by size:
1977 1452 1215 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2546 2598 2825 3141 513
Size: 552 1481 52 227 316 3229
Fragments arranged by size:
3229 1481 552 316 227 52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5118
Fragments arranged by size:
5118 683 56
BtsI GCAGTG_nn'
Cuts at: 2395 2632 3117 3373 4167 4187 2395
Size: 237 485 256 794 20 4065
Fragments arranged by size:
4065 794 485 256 237 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5857
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5822
DraI TTT'AAA
Cuts at: 3161 3161
Size: 5857
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2240 4864 809
Size: 507 924 2624 1802
Fragments arranged by size:
2624 1802 924 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2127 2145 2592
Size: 54 1425 50 275 18 297 18 447
Cuts at: 2592 2889 2919 2961 3078 3304 4193 5822 8
Size: 297 30 42 117 226 889 1629 43
Fragments arranged by size:
1629 1425 889 447 297 297 275 226
117 54 50 43 42 30 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 3078 3304 1537
Size: 275 1266 226 4090
Fragments arranged by size:
4090 1266 275 226
EarI CTCTTCn'nnn_
Cuts at: 1353 2067 3787 1353
Size: 714 1720 3423
Fragments arranged by size:
3423 1720 714
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3023 4444 4818 4964 387
Size: 1064 138 1434 1421 374 146 1280
Fragments arranged by size:
1434 1421 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3920 4517 5304 5572 1239
Size: 393 2288 597 787 268 1524
Fragments arranged by size:
2288 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2768 2768
Size: 5857
EcoRI G'AATT_C
Cuts at: 3166 3544 3166
Size: 378 5479
FspI TGC'GCA
Cuts at: 4363 4363
Size: 5857
HaeII r_GCGC'y
Cuts at: 1678 2328 2441 2459 2473 2495 2507 2559 2588
Size: 650 113 18 14 22 12 52 29
Cuts at: 2588 2606 2754 2781 5004 1678
Size: 18 148 27 2223 2531
Fragments arranged by size:
2531 2223 650 148 113 52 29 27
22 18 18 14 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4498 4530 4572 4604 1373
Size: 32 135 32 2926 32 42 32 2626
Fragments arranged by size:
2926 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3201 3447 669
Size: 988 281 1263 246 3079
Fragments arranged by size:
3079 1263 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5857
HpaI GTT'AAC
Cuts at: 3447 3447
Size: 5857
KasI G'GCGC_C
Cuts at: 2437 2469 2555 2750 2777 2437
Size: 32 86 195 27 5517
Fragments arranged by size:
5517 195 86 32 27
KpnI G_GTAC'C
Cuts at: 3182 3182
Size: 5857
MfeI C'AATT_G
Cuts at: 3456 3456
Size: 5857
MluI A'CGCG_T
Cuts at: 5733 5733
Size: 5857
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4971 5155 1305
Size: 3666 184 2007
Fragments arranged by size:
3666 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2129 2147 2891 2921 10
Size: 54 1425 343 297 18 744 30 2946
Fragments arranged by size:
2946 1425 744 343 297 54 30 18
MslI CAynn'nnrTG
Cuts at: 518 1166 2102 2905 3101 3815 4174 4333 518
Size: 648 936 803 196 714 359 159 2042
Fragments arranged by size:
2042 936 803 714 648 359 196 159
MspA1I CmG'CkG
Cuts at: 1606 2548 2600 2827 2839 2850 2925 2967 3004
Size: 942 52 227 12 11 75 42 37
Cuts at: 3004 3083 3143 3273 3950 5098 5343 1606
Size: 79 60 130 677 1148 245 2120
Fragments arranged by size:
2120 1148 942 677 245 227 130 79
75 60 52 42 37 12 11
NaeI GCC'GGC
Cuts at: 2093 2093
Size: 5857
NarI GG'CG_CC
Cuts at: 2438 2470 2556 2751 2778 2438
Size: 32 86 195 27 5517
Fragments arranged by size:
5517 195 86 32 27
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5305
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5857
NgoMIV G'CCGG_C
Cuts at: 2091 2091
Size: 5857
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5857
NotI GC'GGCC_GC
Cuts at: 3078 3304 3078
Size: 226 5631
NspI r_CATG'y
Cuts at: 1132 1229 2488 3215 4760 1132
Size: 97 1259 727 1545 2229
Fragments arranged by size:
2229 1545 1259 727 97
PasI CC'CwG_GG
Cuts at: 1348 2512 2538 2946 1348
Size: 1164 26 408 4259
Fragments arranged by size:
4259 1164 408 26
PciI A'CATG_T
Cuts at: 4756 4756
Size: 5857
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5857
PmeI GTTT'AAAC
Cuts at: 3161 3161
Size: 5857
PmlI CAC'GTG
Cuts at: 3015 3015
Size: 5857
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3926 3958 5439 5471 3926
Size: 32 1481 32 4312
Fragments arranged by size:
4312 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5857
PsiI TTA'TAA
Cuts at: 3427 3427
Size: 5857
PspOMI G'GGCC_C
Cuts at: 2767 2767
Size: 5857
PstI C_TGCA'G
Cuts at: 830 2193 3209 830
Size: 1363 1016 3478
Fragments arranged by size:
3478 1363 1016
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5857
PvuII CAG'CTG
Cuts at: 2967 2967
Size: 5857
SacII CC_GC'GG
Cuts at: 2549 2601 2828 3144 2549
Size: 52 227 316 5262
Fragments arranged by size:
5262 316 227 52
SalI G'TCGA_C
Cuts at: 1936 3199 1936
Size: 1263 4594
SbfI CC_TGCA'GG
Cuts at: 3209 3209
Size: 5857
ScaI AGT'ACT
Cuts at: 4105 5817 4105
Size: 1712 4145
SfcI C'TryA_G
Cuts at: 826 1045 2189 3205 4340 5021 5212 826
Size: 219 1144 1016 1135 681 191 1471
Fragments arranged by size:
1471 1144 1135 1016 681 219 191
SfoI GGC'GCC
Cuts at: 2439 2471 2557 2752 2779 2439
Size: 32 86 195 27 5517
Fragments arranged by size:
5517 195 86 32 27
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5857
SgrAI Cr'CCGG_yG
Cuts at: 1700 2042 1700
Size: 342 5515
SmaI CCC'GGG
Cuts at: 1872 2435 2748 3184 1872
Size: 563 313 436 4545
Fragments arranged by size:
4545 563 436 313
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3961 4862 5124 820
Size: 197 926 2018 901 262 1553
Fragments arranged by size:
2018 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3648 493
Size: 3155 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5857
SphI G_CATG'C
Cuts at: 1132 2488 3215 1132
Size: 1356 727 3774
Fragments arranged by size:
3774 1356 727
SspI AAT'ATT
Cuts at: 5 52 3781 5
Size: 47 3729 2081
Fragments arranged by size:
3729 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5857
StyI C'CwwG_G
Cuts at: 513 1065 3296 513
Size: 552 2231 3074
Fragments arranged by size:
3074 2231 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2899 3884 3901 4054 4239 4684 325
Size: 1255 1319 985 17 153 185 445 1498
Fragments arranged by size:
1498 1319 1255 985 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4103 5815 96
Size: 274 80 33 51 1443 2126 1712 138
Fragments arranged by size:
2126 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2364 2396 2556 2588 5817 5849 2364
Size: 32 160 32 3229 32 2372
Fragments arranged by size:
3229 2372 160 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5857
XbaI T'CTAG_A
Cuts at: 3193 3193
Size: 5857
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2899 2899
Size: 5857
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5857
XmaI C'CCGG_G
Cuts at: 1870 2433 2746 3182 1870
Size: 563 313 436 4545
Fragments arranged by size:
4545 563 436 313
XmnI GAAnn'nnTTC
Cuts at: 3986 3986
Size: 5857
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3665 276
Size: 53 83 186 936 2131 2468
Fragments arranged by size:
2468 2131 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI
FspAI NruI NsiI PacI PfoI PpuMI PspXI PsrI
RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI