Restriction map for
pFN21AB8819
(Circular) (Six-base) MAPSORT of: pFN21AB8819.seq Check: 3598 from: 1 to: 5851
pFN21AB8819 5851 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3135 3135
Size: 5851
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3661 278
Size: 53 83 186 936 2125 2468
Fragments arranged by size:
2468 2125 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3194 1937
Size: 1257 4594
Acc65I G'GTAC_C
Cuts at: 3172 3172
Size: 5851
AclI AA'CG_TT
Cuts at: 1563 3978 4351 1563
Size: 2415 373 3063
Fragments arranged by size:
3063 2415 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2669 2978 3914 5298 5566 1632
Size: 1037 309 936 1384 268 1917
Fragments arranged by size:
1917 1384 1037 936 309 268
AflII C'TTAA_G
Cuts at: 820 1017 2302 820
Size: 197 1285 4369
Fragments arranged by size:
4369 1285 197
AflIII A'CryG_T
Cuts at: 1203 4750 5727 1203
Size: 3547 977 1327
Fragments arranged by size:
3547 1327 977
AgeI A'CCGG_T
Cuts at: 4682 4682
Size: 5851
AhdI GACnn_n'nnGTC
Cuts at: 4580 4580
Size: 5851
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5851
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5851
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2387 2419 2387
Size: 32 5819
AlwNI CAG_nnn'CTG
Cuts at: 3594 5166 3594
Size: 1572 4279
ApaI G_GGCC'C
Cuts at: 2368 2368
Size: 5851
ApaLI G'TGCA_C
Cuts at: 3908 5064 3908
Size: 1156 4695
ApoI r'AATT_y
Cuts at: 1493 2242 2546 2966 3160 3386 3538 5647 1493
Size: 749 304 420 194 226 152 2109 1697
Fragments arranged by size:
2109 1697 749 420 304 226 194 152
AseI AT'TA_AT
Cuts at: 160 1032 2256 4405 160
Size: 872 1224 2149 1606
Fragments arranged by size:
2149 1606 1224 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5851
AvaI C'yCGr_G
Cuts at: 1870 1943 3176 1870
Size: 73 1233 4545
Fragments arranged by size:
4545 1233 73
BamHI G'GATC_C
Cuts at: 3181 3548 3181
Size: 367 5484
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2908 3172 4627 618
Size: 325 217 627 1121 264 1455 1842
Fragments arranged by size:
1842 1455 1121 627 325 264 217
BanII G_rGCy'C
Cuts at: 1391 2368 3107 3170 1391
Size: 977 739 63 4072
Fragments arranged by size:
4072 977 739 63
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4880
BbvCI CC'TCA_GC
Cuts at: 2568 2876 2568
Size: 308 5543
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4042 4076 1903
Size: 34 2105 34 3678
Fragments arranged by size:
3678 2105 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3743 4953 3743
Size: 1210 4641
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5851
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2181 2765 4462
Size: 107 122 71 631 605 509 584 1697
Cuts at: 4462 136
Size: 1525
Fragments arranged by size:
1697 1525 631 605 584 509 122 107
71
BglII A'GATC_T
Cuts at: 5846 5846
Size: 5851
BlpI GC'TnA_GC
Cuts at: 3268 3268
Size: 5851
Bme1580I G_kGCm'C
Cuts at: 1790 2368 3912 5068 1790
Size: 578 1544 1156 2573
Fragments arranged by size:
2573 1544 1156 578
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5851
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3582 4540 442
Size: 416 53 83 399 24 2165 958 1753
Fragments arranged by size:
2165 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5851
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2528 2528
Size: 5851
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2334 4511 1239
Size: 1095 2177 2579
Fragments arranged by size:
2579 2177 1095
Bpu10I CC'TnA_GC
Cuts at: 2568 2876 2568
Size: 308 5543
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2036 3976 4841 5139 2036
Size: 1940 865 298 2748
Fragments arranged by size:
2748 1940 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2289 4514 882
Size: 617 790 2225 2219
Fragments arranged by size:
2225 2219 790 617
BsaAI yAC'GTr
Cuts at: 493 1204 2068 3642 493
Size: 711 864 1574 2702
Fragments arranged by size:
2702 1574 864 711
BsaBI GATnn'nnATC
Cuts at: 5845 5845
Size: 5851
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3658 4040 4675 275
Size: 53 83 186 936 2125 382 635 1451
Fragments arranged by size:
2125 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2161 4284 4682 4956 5103 5514 2161
Size: 2123 398 274 147 411 2498
Fragments arranged by size:
2498 2123 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5626
Fragments arranged by size:
5626 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5851
BseYI C'CCAG_C
Cuts at: 5054 5550 5054
Size: 496 5355
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5278
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3301 4062 5090 1540
Size: 275 186 1300 761 1028 2301
Fragments arranged by size:
2301 1300 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2827 3912 3997 5068 2827
Size: 1085 85 1071 3610
Fragments arranged by size:
3610 1085 1071 85
BsmI GAATG_Cn'
Cuts at: 3362 3455 3362
Size: 93 5758
BsmBI CGTCTCn'nnnn_
Cuts at: 2619 2619
Size: 5851
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2368 2827 3107 3170 3912 3997 5068
Size: 399 578 459 280 63 742 85 1071
Cuts at: 5068 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 578 459 399 280 85
63
BspEI T'CCGG_A
Cuts at: 5514 5514
Size: 5851
BspHI T'CATG_A
Cuts at: 3740 5470 3740
Size: 1730 4121
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3135 3206 844
Size: 2291 71 3489
Fragments arranged by size:
3489 2291 71
BsrBI CCG'CTC
Cuts at: 1394 1823 2013 2060 2560 3738 1394
Size: 429 190 47 500 1178 3507
Fragments arranged by size:
3507 1178 500 429 190 47
BsrDI GCAATG_nn'
Cuts at: 65 1248 4346 4520 65
Size: 1183 3098 174 1396
Fragments arranged by size:
3098 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4495 4682 1700
Size: 110 2685 187 2869
Fragments arranged by size:
2869 2685 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5851
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5851
BssSI C'ACGA_G
Cuts at: 2750 3911 4923 2750
Size: 1161 1012 3678
Fragments arranged by size:
3678 1161 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5851
BstBI TT'CG_AA
Cuts at: 4655 4655
Size: 5851
BstEII G'GTnAC_C
Cuts at: 1184 2164 1184
Size: 980 4871
BstXI CCAn_nnnn'nTGG
Cuts at: 3076 3076
Size: 5851
BstYI r'GATC_y
Cuts at: 1972 3181 3548 3933 3950 5402 5488 5500 5846
Size: 1209 367 385 17 1452 86 12 346
Cuts at: 5846 1972
Size: 1977
Fragments arranged by size:
1977 1452 1209 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2211 513
Size: 552 939 207 4153
Fragments arranged by size:
4153 939 552 207
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2436 487
Size: 683 56 1210 3902
Fragments arranged by size:
3902 1210 683 56
BtsI GCAGTG_nn'
Cuts at: 3081 3367 4161 4181 3081
Size: 286 794 20 4751
Fragments arranged by size:
4751 794 286 20
ClaI AT'CG_AT
Cuts at: 1554 2796 1554
Size: 1242 4609
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5816
DraI TTT'AAA
Cuts at: 3155 3155
Size: 5851
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2087 4858 809
Size: 507 771 2771 1802
Fragments arranged by size:
2771 1802 771 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2469 3091 3298
Size: 54 1425 50 275 18 639 622 207
Cuts at: 3298 4187 5816 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 639 622 275 207 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3298 1537
Size: 275 1486 4090
Fragments arranged by size:
4090 1486 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2550 3054 3781 1353
Size: 1197 504 727 3423
Fragments arranged by size:
3423 1197 727 504
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2261 2875 4438 4812 4958 387
Size: 1064 138 672 614 1563 374 146 1280
Fragments arranged by size:
1563 1280 1064 672 614 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2334 2669 2978 3914 4511 5298 5566
Size: 393 702 335 309 936 597 787 268
Cuts at: 5566 1239
Size: 1524
Fragments arranged by size:
1524 936 787 702 597 393 335 309
268
EcoNI CCTnn'n_nnAGG
Cuts at: 3131 3131
Size: 5851
EcoO109I rG'GnC_Cy
Cuts at: 2364 2365 2364
Size: 1 5850
EcoRI G'AATT_C
Cuts at: 3160 3538 3160
Size: 378 5473
FspI TGC'GCA
Cuts at: 4357 4357
Size: 5851
HaeII r_GCGC'y
Cuts at: 1678 4998 1678
Size: 3320 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2417 2449 4492 4524 4566
Size: 32 135 32 845 32 2043 32 42
Cuts at: 4566 4598 1373
Size: 32 2626
Fragments arranged by size:
2626 2043 845 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2197 3195 3441 669
Size: 988 281 259 998 246 3079
Fragments arranged by size:
3079 998 988 281 259 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5851
HpaI GTT'AAC
Cuts at: 2197 3441 2197
Size: 1244 4607
KpnI G_GTAC'C
Cuts at: 3176 3176
Size: 5851
MfeI C'AATT_G
Cuts at: 3450 3450
Size: 5851
MluI A'CGCG_T
Cuts at: 5727 5727
Size: 5851
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4965 5149 1305
Size: 3660 184 2007
Fragments arranged by size:
3660 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4029
Fragments arranged by size:
4029 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2499 3809 4168 4327 518
Size: 648 1333 1310 359 159 2042
Fragments arranged by size:
2042 1333 1310 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2213 2474 3267 3944 5092 5337 1606
Size: 607 261 793 677 1148 245 2120
Fragments arranged by size:
2120 1148 793 677 607 261 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4360
Fragments arranged by size:
4360 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5851
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5851
NotI GC'GGCC_GC
Cuts at: 3298 3298
Size: 5851
NspI r_CATG'y
Cuts at: 1132 1229 2104 3122 3209 4754 1132
Size: 97 875 1018 87 1545 2229
Fragments arranged by size:
2229 1545 1018 875 97 87
PasI CC'CwG_GG
Cuts at: 1348 2179 2903 3127 1348
Size: 831 724 224 4072
Fragments arranged by size:
4072 831 724 224
PciI A'CATG_T
Cuts at: 4750 4750
Size: 5851
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5851
PmeI GTTT'AAAC
Cuts at: 3155 3155
Size: 5851
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3920 3952 5433 5465 3920
Size: 32 1481 32 4306
Fragments arranged by size:
4306 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5851
PsiI TTA'TAA
Cuts at: 3421 3421
Size: 5851
PspOMI G'GGCC_C
Cuts at: 2364 2364
Size: 5851
PstI C_TGCA'G
Cuts at: 830 3203 830
Size: 2373 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5851
SacII CC_GC'GG
Cuts at: 2214 2214
Size: 5851
SalI G'TCGA_C
Cuts at: 1936 3193 1936
Size: 1257 4594
SapI GCTCTTCn'nnn_
Cuts at: 2550 2550
Size: 5851
SbfI CC_TGCA'GG
Cuts at: 3203 3203
Size: 5851
ScaI AGT'ACT
Cuts at: 4099 5811 4099
Size: 1712 4139
SfcI C'TryA_G
Cuts at: 826 1045 3199 4334 5015 5206 826
Size: 219 2154 1135 681 191 1471
Fragments arranged by size:
2154 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5851
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5851
SmaI CCC'GGG
Cuts at: 1872 3178 1872
Size: 1306 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2051 2302 3955 4856 5118 820
Size: 197 926 108 251 1653 901 262 1553
Fragments arranged by size:
1653 1553 926 901 262 251 197 108
SnaBI TAC'GTA
Cuts at: 493 3642 493
Size: 3149 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5851
SphI G_CATG'C
Cuts at: 1132 3209 1132
Size: 2077 3774
SspI AAT'ATT
Cuts at: 5 52 3775 5
Size: 47 3723 2081
Fragments arranged by size:
3723 2081 47
StuI AGG'CCT
Cuts at: 1517 2122 1517
Size: 605 5246
StyI C'CwwG_G
Cuts at: 513 1065 2004 3290 513
Size: 552 939 1286 3074
Fragments arranged by size:
3074 1286 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2060 3878 3895 4048 4233 4678 325
Size: 1255 480 1818 17 153 185 445 1498
Fragments arranged by size:
1818 1498 1255 480 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4097 5809 96
Size: 274 80 33 51 1443 2120 1712 138
Fragments arranged by size:
2120 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2207 2239 3021 3053 5811 5843 2207
Size: 32 782 32 2758 32 2215
Fragments arranged by size:
2758 2215 782 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5851
XbaI T'CTAG_A
Cuts at: 3187 3187
Size: 5851
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2531 3004 2531
Size: 473 5378
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5851
XmaI C'CCGG_G
Cuts at: 1870 3176 1870
Size: 1306 4545
XmnI GAAnn'nnTTC
Cuts at: 3980 3980
Size: 5851
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3659 276
Size: 53 83 186 936 2125 2468
Fragments arranged by size:
2468 2125 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI SacII SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I
Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspXI PsrI PvuII RsrII SacI SanDI SexAI
SfiI SfoI SrfI SwaI