Restriction map for
pFN21AB8818
(Circular) (Six-base) MAPSORT of: pFN21AB8818.seq Check: 1208 from: 1 to: 5848
pFN21AB8818 5848 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3658 278
Size: 53 83 186 936 2122 2468
Fragments arranged by size:
2468 2122 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3191 1937
Size: 1254 4594
Acc65I G'GTAC_C
Cuts at: 3169 3169
Size: 5848
AclI AA'CG_TT
Cuts at: 1563 3975 4348 1563
Size: 2412 373 3063
Fragments arranged by size:
3063 2412 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2022 3057 3911 5295 5563 1632
Size: 390 1035 854 1384 268 1917
Fragments arranged by size:
1917 1384 1035 854 390 268
AflII C'TTAA_G
Cuts at: 820 1017 2585 820
Size: 197 1568 4083
Fragments arranged by size:
4083 1568 197
AflIII A'CryG_T
Cuts at: 1203 2415 4747 5724 1203
Size: 1212 2332 977 1327
Fragments arranged by size:
2332 1327 1212 977
AgeI A'CCGG_T
Cuts at: 4679 4679
Size: 5848
AhdI GACnn_n'nnGTC
Cuts at: 4577 4577
Size: 5848
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5848
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2450 1184
Size: 1266 4582
AlwNI CAG_nnn'CTG
Cuts at: 2049 3591 5163 2049
Size: 1542 1572 2734
Fragments arranged by size:
2734 1572 1542
ApaLI G'TGCA_C
Cuts at: 3905 5061 3905
Size: 1156 4692
ApoI r'AATT_y
Cuts at: 1493 2263 2274 2765 3077 3157 3383 3535 5644
Size: 770 11 491 312 80 226 152 2109
Cuts at: 5644 1493
Size: 1697
Fragments arranged by size:
2109 1697 770 491 312 226 152 80
11
AseI AT'TA_AT
Cuts at: 160 1032 2472 4402 160
Size: 872 1440 1930 1606
Fragments arranged by size:
1930 1606 1440 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5848
AvaI C'yCGr_G
Cuts at: 1870 1943 3173 1870
Size: 73 1230 4545
Fragments arranged by size:
4545 1230 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3096 3129 3096
Size: 33 5815
BamHI G'GATC_C
Cuts at: 3178 3545 3178
Size: 367 5481
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3169 4624 618
Size: 325 217 627 1382 1455 1842
Fragments arranged by size:
1842 1455 1382 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3167 1391
Size: 1776 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4877
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4039 4073 1903
Size: 34 2102 34 3678
Fragments arranged by size:
3678 2102 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3083 3740 4950 3083
Size: 657 1210 3981
Fragments arranged by size:
3981 1210 657
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5848
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4459 136
Size: 107 122 71 631 605 2787 1525
Fragments arranged by size:
2787 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5843 5843
Size: 5848
BlpI GC'TnA_GC
Cuts at: 3265 3265
Size: 5848
Bme1580I G_kGCm'C
Cuts at: 1790 3909 5065 1790
Size: 2119 1156 2573
Fragments arranged by size:
2573 2119 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5848
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3579 4537 442
Size: 416 53 83 399 24 2162 958 1753
Fragments arranged by size:
2162 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5848
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4508 1239
Size: 3269 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3973 4838 5136 3973
Size: 865 298 4685
Fragments arranged by size:
4685 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4511 882
Size: 617 3012 2219
Fragments arranged by size:
3012 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3639 493
Size: 711 2435 2702
Fragments arranged by size:
2702 2435 711
BsaBI GATnn'nnATC
Cuts at: 5842 5842
Size: 5848
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3655 4037 4672 275
Size: 53 83 186 936 2122 382 635 1451
Fragments arranged by size:
2122 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4281 4679 4953 5100 5511 4281
Size: 398 274 147 411 4618
Fragments arranged by size:
4618 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5623
Fragments arranged by size:
5623 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2050 1186
Size: 864 4984
BseYI C'CCAG_C
Cuts at: 5051 5547 5051
Size: 496 5352
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5275
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3298 4059 5087 1540
Size: 275 186 1297 761 1028 2301
Fragments arranged by size:
2301 1297 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3909 3994 5065 3909
Size: 85 1071 4692
Fragments arranged by size:
4692 1071 85
BsmI GAATG_Cn'
Cuts at: 2076 3359 3452 2076
Size: 1283 93 4472
Fragments arranged by size:
4472 1283 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3167 3909 3994 5065 1391
Size: 399 1377 742 85 1071 2174
Fragments arranged by size:
2174 1377 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5511 5511
Size: 5848
BspHI T'CATG_A
Cuts at: 3737 5467 3737
Size: 1730 4118
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3203 844
Size: 2359 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3735 1394
Size: 429 1912 3507
Fragments arranged by size:
3507 1912 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2295 4343 4517 65
Size: 1183 1047 2048 174 1396
Fragments arranged by size:
2048 1396 1183 1047 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4492 4679 1700
Size: 110 2682 187 2869
Fragments arranged by size:
2869 2682 187 110
BsrGI T'GTAC_A
Cuts at: 96 2418 96
Size: 2322 3526
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5848
BssSI C'ACGA_G
Cuts at: 3908 4920 3908
Size: 1012 4836
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5848
BstBI TT'CG_AA
Cuts at: 4652 4652
Size: 5848
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5848
BstYI r'GATC_y
Cuts at: 1972 2971 3178 3545 3930 3947 5399 5485 5497
Size: 999 207 367 385 17 1452 86 12
Cuts at: 5497 5843 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 999 385 367 346 207 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2145 513
Size: 552 1080 4216
Fragments arranged by size:
4216 1080 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5109
Fragments arranged by size:
5109 683 56
BtsI GCAGTG_nn'
Cuts at: 3123 3364 4158 4178 3123
Size: 241 794 20 4793
Fragments arranged by size:
4793 794 241 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5848
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2342 2377 529
Size: 35 1778 35 4000
Fragments arranged by size:
4000 1778 35 35
DraI TTT'AAA
Cuts at: 3082 3152 3082
Size: 70 5778
DraIII CAC_nnn'GTG
Cuts at: 2372 2613 2372
Size: 241 5607
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4855 809
Size: 507 3539 1802
Fragments arranged by size:
3539 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3295 4184 5813
Size: 54 1425 50 275 18 1465 889 1629
Cuts at: 5813 8
Size: 43
Fragments arranged by size:
1629 1465 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3295 1537
Size: 275 1483 4090
Fragments arranged by size:
4090 1483 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3778 1353
Size: 2425 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4435 4809 4955 387
Size: 1064 138 2846 374 146 1280
Fragments arranged by size:
2846 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2022 3057 3911 4508 5295 5563 1239
Size: 393 390 1035 854 597 787 268 1524
Fragments arranged by size:
1524 1035 854 787 597 393 390 268
EcoRI G'AATT_C
Cuts at: 3157 3535 3157
Size: 378 5470
FspI TGC'GCA
Cuts at: 4354 4354
Size: 5848
HaeII r_GCGC'y
Cuts at: 1678 4995 1678
Size: 3317 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2533 2565 4489 4521 4563
Size: 32 135 32 961 32 1924 32 42
Cuts at: 4563 4595 1373
Size: 32 2626
Fragments arranged by size:
2626 1924 961 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2175 3192 3438 669
Size: 988 281 237 1017 246 3079
Fragments arranged by size:
3079 1017 988 281 246 237
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5848
HpaI GTT'AAC
Cuts at: 2175 3438 2175
Size: 1263 4585
KpnI G_GTAC'C
Cuts at: 3173 3173
Size: 5848
MfeI C'AATT_G
Cuts at: 3447 3447
Size: 5848
MluI A'CGCG_T
Cuts at: 5724 5724
Size: 5848
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4962 5146 1305
Size: 3657 184 2007
Fragments arranged by size:
3657 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4026
Fragments arranged by size:
4026 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2024 2672 3806 4165 4324 518
Size: 648 858 648 1134 359 159 2042
Fragments arranged by size:
2042 1134 858 648 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2049 3264 3941 5089 5334 1606
Size: 443 1215 677 1148 245 2120
Fragments arranged by size:
2120 1215 1148 677 443 245
NcoI C'CATG_G
Cuts at: 513 1065 2145 513
Size: 552 1080 4216
Fragments arranged by size:
4216 1080 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5848
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5848
NotI GC'GGCC_GC
Cuts at: 3295 3295
Size: 5848
NspI r_CATG'y
Cuts at: 1132 1229 2419 3206 4751 1132
Size: 97 1190 787 1545 2229
Fragments arranged by size:
2229 1545 1190 787 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5848
PciI A'CATG_T
Cuts at: 2415 4747 2415
Size: 2332 3516
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5848
PmeI GTTT'AAAC
Cuts at: 3152 3152
Size: 5848
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3917 3949 5430 5462 3917
Size: 32 1481 32 4303
Fragments arranged by size:
4303 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5848
PsiI TTA'TAA
Cuts at: 2081 3418 2081
Size: 1337 4511
PstI C_TGCA'G
Cuts at: 830 3200 830
Size: 2370 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5848
PvuII CAG'CTG
Cuts at: 2049 2049
Size: 5848
SalI G'TCGA_C
Cuts at: 1936 3190 1936
Size: 1254 4594
SbfI CC_TGCA'GG
Cuts at: 3200 3200
Size: 5848
ScaI AGT'ACT
Cuts at: 3145 4096 5808 3145
Size: 951 1712 3185
Fragments arranged by size:
3185 1712 951
SfcI C'TryA_G
Cuts at: 826 1045 2793 3196 4331 5012 5203 826
Size: 219 1748 403 1135 681 191 1471
Fragments arranged by size:
1748 1471 1135 681 403 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5848
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5848
SmaI CCC'GGG
Cuts at: 1872 3175 1872
Size: 1303 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2585 3952 4853 5115 820
Size: 197 926 642 1367 901 262 1553
Fragments arranged by size:
1553 1367 926 901 642 262 197
SnaBI TAC'GTA
Cuts at: 493 3639 493
Size: 3146 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5848
SphI G_CATG'C
Cuts at: 1132 3206 1132
Size: 2074 3774
SspI AAT'ATT
Cuts at: 5 52 3772 5
Size: 47 3720 2081
Fragments arranged by size:
3720 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5848
StyI C'CwwG_G
Cuts at: 513 1065 2145 3287 513
Size: 552 1080 1142 3074
Fragments arranged by size:
3074 1142 1080 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2466 3875 3892 4045 4230 4675 325
Size: 1255 886 1409 17 153 185 445 1498
Fragments arranged by size:
1498 1409 1255 886 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2418 2815 3143
Size: 274 80 33 51 1443 441 397 328
Cuts at: 3143 4094 5806 96
Size: 951 1712 138
Fragments arranged by size:
1712 1443 951 441 397 328 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2356 2388 5808 5840 2356
Size: 32 3420 32 2364
Fragments arranged by size:
3420 2364 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5848
XbaI T'CTAG_A
Cuts at: 3184 3184
Size: 5848
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5848
XmaI C'CCGG_G
Cuts at: 1870 3173 1870
Size: 1303 4545
XmnI GAAnn'nnTTC
Cuts at: 2916 3977 2916
Size: 1061 4787
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3656 276
Size: 53 83 186 936 2122 2468
Fragments arranged by size:
2468 2122 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI