Restriction map for
pFN21AB8812
(Circular) (Six-base) MAPSORT of: pFN21AB8812.seq Check: 2927 from: 1 to: 5797
pFN21AB8812 5797 bp
With 182 enzymes: SgfI *
February 27, 2009 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3607 278
Size: 53 83 186 936 2071 2468
Fragments arranged by size:
2468 2071 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3140 1937
Size: 1203 4594
Acc65I G'GTAC_C
Cuts at: 2299 3032 3118 2299
Size: 733 86 4978
Fragments arranged by size:
4978 733 86
AclI AA'CG_TT
Cuts at: 1563 3924 4297 1563
Size: 2361 373 3063
Fragments arranged by size:
3063 2361 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3860 5244 5512 1632
Size: 2228 1384 268 1917
Fragments arranged by size:
2228 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5600
AflIII A'CryG_T
Cuts at: 1203 2376 2834 4696 5673 1203
Size: 1173 458 1862 977 1327
Fragments arranged by size:
1862 1327 1173 977 458
AgeI A'CCGG_T
Cuts at: 4628 4628
Size: 5797
AhdI GACnn_n'nnGTC
Cuts at: 2101 4526 2101
Size: 2425 3372
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5797
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5797
AlwNI CAG_nnn'CTG
Cuts at: 2779 3540 5112 2779
Size: 761 1572 3464
Fragments arranged by size:
3464 1572 761
ApaI G_GGCC'C
Cuts at: 2036 2805 2036
Size: 769 5028
ApaLI G'TGCA_C
Cuts at: 3854 5010 3854
Size: 1156 4641
ApoI r'AATT_y
Cuts at: 1493 3106 3332 3484 5593 1493
Size: 1613 226 152 2109 1697
Fragments arranged by size:
2109 1697 1613 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4351 160
Size: 872 3319 1606
Fragments arranged by size:
3319 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5797
AvaI C'yCGr_G
Cuts at: 1870 1943 3122 1870
Size: 73 1179 4545
Fragments arranged by size:
4545 1179 73
BamHI G'GATC_C
Cuts at: 2134 3127 3494 2134
Size: 993 367 4437
Fragments arranged by size:
4437 993 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2019 2299 2318 2436 3032
Size: 325 217 627 232 280 19 118 596
Cuts at: 3032 3118 4573 618
Size: 86 1455 1842
Fragments arranged by size:
1842 1455 627 596 325 280 232 217
118 86 19
BanII G_rGCy'C
Cuts at: 1391 2036 2455 2514 2805 2814 3116 1391
Size: 645 419 59 291 9 302 4072
Fragments arranged by size:
4072 645 419 302 291 59 9
BbeI G_GCGC'C
Cuts at: 2440 2440
Size: 5797
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4826
BbvCI CC'TCA_GC
Cuts at: 2625 2625
Size: 5797
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2018 2021 2052 3988 4022 1903
Size: 34 50 31 3 31 1936 34 3678
Fragments arranged by size:
3678 1936 50 34 34 31 31 3
BciVI GTATCCnnnnn_n'
Cuts at: 3689 4899 3689
Size: 1210 4587
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5797
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2445 2717 4408
Size: 107 122 71 631 605 773 272 1691
Cuts at: 4408 136
Size: 1525
Fragments arranged by size:
1691 1525 773 631 605 272 122 107
71
BglII A'GATC_T
Cuts at: 5792 5792
Size: 5797
BlpI GC'TnA_GC
Cuts at: 3214 3214
Size: 5797
Bme1580I G_kGCm'C
Cuts at: 1790 2036 2259 2805 3858 5014 1790
Size: 246 223 546 1053 1156 2573
Fragments arranged by size:
2573 1156 1053 546 246 223
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5797
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2810 2820 3528
Size: 416 53 83 399 24 1393 10 708
Cuts at: 3528 4486 442
Size: 958 1753
Fragments arranged by size:
1753 1393 958 708 416 399 83 53
24 10
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5797
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2587 4457 1239
Size: 1348 1870 2579
Fragments arranged by size:
2579 1870 1348
Bpu10I CC'TnA_GC
Cuts at: 2625 2625
Size: 5797
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2513 3922 4787 5085 2513
Size: 1409 865 298 3225
Fragments arranged by size:
3225 1409 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2935 3006 3056 4460 882
Size: 617 1436 71 50 1404 2219
Fragments arranged by size:
2219 1436 1404 617 71 50
BsaAI yAC'GTr
Cuts at: 493 1204 3588 493
Size: 711 2384 2702
Fragments arranged by size:
2702 2384 711
BsaBI GATnn'nnATC
Cuts at: 5791 5791
Size: 5797
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2102 2437 3604 3986
Size: 53 83 186 936 569 335 1167 382
Cuts at: 3986 4621 275
Size: 635 1451
Fragments arranged by size:
1451 1167 936 635 569 382 335 186
83 53
BsaWI w'CCGG_w
Cuts at: 4230 4628 4902 5049 5460 4230
Size: 398 274 147 411 4567
Fragments arranged by size:
4567 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5572
Fragments arranged by size:
5572 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2897 3002 3005 1186
Size: 1711 105 3 3978
Fragments arranged by size:
3978 1711 105 3
BseYI C'CCAG_C
Cuts at: 2252 2264 2295 2361 2365 2874 2950 5000 5496
Size: 12 31 66 4 509 76 2050 496
Cuts at: 5496 2252
Size: 2553
Fragments arranged by size:
2553 2050 509 496 76 66 31 12
4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5224
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3247 4008 5036 1540
Size: 275 186 1246 761 1028 2301
Fragments arranged by size:
2301 1246 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2204 2397 2514 2764 2795 3858 3943 5014 2204
Size: 193 117 250 31 1063 85 1071 2987
Fragments arranged by size:
2987 1071 1063 250 193 117 85 31
BsmI GAATG_Cn'
Cuts at: 2040 3308 3401 2040
Size: 1268 93 4436
Fragments arranged by size:
4436 1268 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2109 2109
Size: 5797
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2036 2204 2259 2397 2455 2514 2764
Size: 399 246 168 55 138 58 59 250
Cuts at: 2764 2795 2805 2814 3116 3858 3943 5014 1391
Size: 31 10 9 302 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 302 250 246 168
138 85 59 58 55 31 10 9
BspEI T'CCGG_A
Cuts at: 5460 5460
Size: 5797
BspHI T'CATG_A
Cuts at: 3686 5416 3686
Size: 1730 4067
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2239 2496 3152 844
Size: 1395 257 656 3489
Fragments arranged by size:
3489 1395 656 257
BsrBI CCG'CTC
Cuts at: 1394 1823 2149 3684 1394
Size: 429 326 1535 3507
Fragments arranged by size:
3507 1535 429 326
BsrDI GCAATG_nn'
Cuts at: 65 1248 4292 4466 65
Size: 1183 3044 174 1396
Fragments arranged by size:
3044 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2016 4441 4628 1700
Size: 110 206 2425 187 2869
Fragments arranged by size:
2869 2425 206 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5797
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5797
BssSI C'ACGA_G
Cuts at: 2010 3857 4869 2010
Size: 1847 1012 2938
Fragments arranged by size:
2938 1847 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5797
BstBI TT'CG_AA
Cuts at: 4601 4601
Size: 5797
BstEII G'GTnAC_C
Cuts at: 1184 2578 1184
Size: 1394 4403
BstYI r'GATC_y
Cuts at: 1972 2134 2427 2524 3127 3494 3879 3896 5348
Size: 162 293 97 603 367 385 17 1452
Cuts at: 5348 5434 5446 5792 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 603 385 367 346 293 162
97 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2432 3028 2432
Size: 596 5201
BtgI C'CryG_G
Cuts at: 513 1065 2081 2187 2336 2715 513
Size: 552 1016 106 149 379 3595
Fragments arranged by size:
3595 1016 552 379 149 106
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5058
Fragments arranged by size:
5058 683 56
BtsI GCAGTG_nn'
Cuts at: 3313 4107 4127 3313
Size: 794 20 4983
Fragments arranged by size:
4983 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5797
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5762
DraI TTT'AAA
Cuts at: 3101 3101
Size: 5797
DraIII CAC_nnn'GTG
Cuts at: 2172 2284 2172
Size: 112 5685
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4804 809
Size: 507 3488 1802
Fragments arranged by size:
3488 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2014 2718 3244
Size: 54 1425 50 275 18 184 704 526
Cuts at: 3244 4133 5762 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 704 526 275 184 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3244 1537
Size: 275 1432 4090
Fragments arranged by size:
4090 1432 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2192 2519 2875 3727 1353
Size: 839 327 356 852 3423
Fragments arranged by size:
3423 852 839 356 327
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4384 4758 4904 387
Size: 1064 138 2795 374 146 1280
Fragments arranged by size:
2795 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2512 2512
Size: 5797
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2587 3860 4457 5244 5512 1239
Size: 393 955 1273 597 787 268 1524
Fragments arranged by size:
1524 1273 955 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2032 2033 2243 2485 2668 3055 2032
Size: 1 210 242 183 387 4774
Fragments arranged by size:
4774 387 242 210 183 1
EcoRI G'AATT_C
Cuts at: 3106 3484 3106
Size: 378 5419
FspI TGC'GCA
Cuts at: 4303 4303
Size: 5797
HaeII r_GCGC'y
Cuts at: 1678 2440 4944 1678
Size: 762 2504 2531
Fragments arranged by size:
2531 2504 762
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2087 2119 2363 2395 4438
Size: 32 135 32 515 32 244 32 2043
Cuts at: 4438 4470 4512 4544 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2043 515 244 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3141 3387 669
Size: 988 281 1203 246 3079
Fragments arranged by size:
3079 1203 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5797
HpaI GTT'AAC
Cuts at: 3387 3387
Size: 5797
KasI G'GCGC_C
Cuts at: 2436 2436
Size: 5797
KpnI G_GTAC'C
Cuts at: 2303 3036 3122 2303
Size: 733 86 4978
Fragments arranged by size:
4978 733 86
MfeI C'AATT_G
Cuts at: 3396 3396
Size: 5797
MluI A'CGCG_T
Cuts at: 5673 5673
Size: 5797
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2766 2895 4911 5095 1305
Size: 1461 129 2016 184 2007
Fragments arranged by size:
2016 2007 1461 184 129
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2720 10
Size: 54 1425 343 888 3087
Fragments arranged by size:
3087 1425 888 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2080 3755 4114 4273 518
Size: 648 914 1675 359 159 2042
Fragments arranged by size:
2042 1675 914 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2365 2611 2629 2779 3213 3890 5038 5283
Size: 759 246 18 150 434 677 1148 245
Cuts at: 5283 1606
Size: 2120
Fragments arranged by size:
2120 1148 759 677 434 246 245 150
18
NarI GG'CG_CC
Cuts at: 2437 2437
Size: 5797
NcoI C'CATG_G
Cuts at: 513 1065 2081 2187 2715 513
Size: 552 1016 106 528 3595
Fragments arranged by size:
3595 1016 552 528 106
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5797
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5797
NotI GC'GGCC_GC
Cuts at: 3244 3244
Size: 5797
NspI r_CATG'y
Cuts at: 1132 1229 2263 2380 2838 3155 4700 1132
Size: 97 1034 117 458 317 1545 2229
Fragments arranged by size:
2229 1545 1034 458 317 117 97
PasI CC'CwG_GG
Cuts at: 1348 2798 1348
Size: 1450 4347
PciI A'CATG_T
Cuts at: 2376 2834 4696 2376
Size: 458 1862 3477
Fragments arranged by size:
3477 1862 458
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2284 2575 1108
Size: 1176 291 4330
Fragments arranged by size:
4330 1176 291
PfoI T'CCnGG_A
Cuts at: 2959 2959
Size: 5797
PmeI GTTT'AAAC
Cuts at: 3101 3101
Size: 5797
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3866 3898 5379 5411 3866
Size: 32 1481 32 4252
Fragments arranged by size:
4252 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2243 2485 2243
Size: 242 5555
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5797
PsiI TTA'TAA
Cuts at: 3367 3367
Size: 5797
PspOMI G'GGCC_C
Cuts at: 2032 2801 2032
Size: 769 5028
PstI C_TGCA'G
Cuts at: 830 2236 3149 830
Size: 1406 913 3478
Fragments arranged by size:
3478 1406 913
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5797
PvuII CAG'CTG
Cuts at: 2365 2611 2629 2779 2365
Size: 246 18 150 5383
Fragments arranged by size:
5383 246 150 18
SacI G_AGCT'C
Cuts at: 2514 2514
Size: 5797
SalI G'TCGA_C
Cuts at: 1936 3139 1936
Size: 1203 4594
SanDI GG'GwC_CC
Cuts at: 2243 2243
Size: 5797
SapI GCTCTTCn'nnn_
Cuts at: 2192 2519 2192
Size: 327 5470
SbfI CC_TGCA'GG
Cuts at: 3149 3149
Size: 5797
ScaI AGT'ACT
Cuts at: 4045 5757 4045
Size: 1712 4085
SfcI C'TryA_G
Cuts at: 826 1045 2232 3145 4280 4961 5152 826
Size: 219 1187 913 1135 681 191 1471
Fragments arranged by size:
1471 1187 1135 913 681 219 191
SfoI GGC'GCC
Cuts at: 2438 2438
Size: 5797
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5797
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5797
SmaI CCC'GGG
Cuts at: 1872 3124 1872
Size: 1252 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2528 3901 4802 5064 820
Size: 197 926 585 1373 901 262 1553
Fragments arranged by size:
1553 1373 926 901 585 262 197
SnaBI TAC'GTA
Cuts at: 493 3588 493
Size: 3095 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5797
SphI G_CATG'C
Cuts at: 1132 3155 1132
Size: 2023 3774
SspI AAT'ATT
Cuts at: 5 52 3721 5
Size: 47 3669 2081
Fragments arranged by size:
3669 2081 47
StuI AGG'CCT
Cuts at: 1517 2459 1517
Size: 942 4855
StyI C'CwwG_G
Cuts at: 513 1065 2081 2187 2715 2784 2805 3236 513
Size: 552 1016 106 528 69 21 431 3074
Fragments arranged by size:
3074 1016 552 528 431 106 69 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3824 3841 3994 4179 4624 325
Size: 1255 2244 17 153 185 445 1498
Fragments arranged by size:
2244 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4043 5755 96
Size: 274 80 33 51 1443 2066 1712 138
Fragments arranged by size:
2066 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2156 2188 5757 5789 2156
Size: 32 3569 32 2164
Fragments arranged by size:
3569 2164 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5797
XbaI T'CTAG_A
Cuts at: 2753 3133 2753
Size: 380 5417
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5797
XmaI C'CCGG_G
Cuts at: 1870 3122 1870
Size: 1252 4545
XmnI GAAnn'nnTTC
Cuts at: 2538 3926 2538
Size: 1388 4409
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3605 276
Size: 53 83 186 936 2071 2468
Fragments arranged by size:
2468 2071 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BstXI BstZ17I EcoNI EcoRV FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI
RsrII SacII SexAI SfiI SrfI SwaI XcmI