Restriction map for
pFN21AB8810
(Circular) (Six-base) MAPSORT of: pFN21AB8810.seq Check: 5476 from: 1 to: 5788
pFN21AB8810 5788 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2345 2345
Size: 5788
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3598 278
Size: 53 83 186 936 2062 2468
Fragments arranged by size:
2468 2062 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3131 1937
Size: 1194 4594
Acc65I G'GTAC_C
Cuts at: 3109 3109
Size: 5788
AclI AA'CG_TT
Cuts at: 1563 3915 4288 1563
Size: 2352 373 3063
Fragments arranged by size:
3063 2352 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2659 3042 3851 5235 5503 1632
Size: 1027 383 809 1384 268 1917
Fragments arranged by size:
1917 1384 1027 809 383 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5591
AflIII A'CryG_T
Cuts at: 1203 4687 5664 1203
Size: 3484 977 1327
Fragments arranged by size:
3484 1327 977
AgeI A'CCGG_T
Cuts at: 4619 4619
Size: 5788
AhdI GACnn_n'nnGTC
Cuts at: 4517 4517
Size: 5788
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5788
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5788
AlwNI CAG_nnn'CTG
Cuts at: 3020 3531 5103 3020
Size: 511 1572 3705
Fragments arranged by size:
3705 1572 511
ApaLI G'TGCA_C
Cuts at: 2405 3845 5001 2405
Size: 1440 1156 3192
Fragments arranged by size:
3192 1440 1156
ApoI r'AATT_y
Cuts at: 1493 2218 3028 3097 3323 3475 5584 1493
Size: 725 810 69 226 152 2109 1697
Fragments arranged by size:
2109 1697 810 725 226 152 69
AseI AT'TA_AT
Cuts at: 160 1032 4342 160
Size: 872 3310 1606
Fragments arranged by size:
3310 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5788
AvaI C'yCGr_G
Cuts at: 1870 1943 3113 1870
Size: 73 1170 4545
Fragments arranged by size:
4545 1170 73
BamHI G'GATC_C
Cuts at: 3118 3485 3118
Size: 367 5421
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3109 4564 618
Size: 325 217 627 1322 1455 1842
Fragments arranged by size:
1842 1455 1322 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2142 3107 1391
Size: 751 965 4072
Fragments arranged by size:
4072 965 751
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2119 3066 928
Size: 971 220 947 3650
Fragments arranged by size:
3650 971 947 220
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3979 4013 1903
Size: 34 2042 34 3678
Fragments arranged by size:
3678 2042 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2565 3680 4890 2565
Size: 1115 1210 3463
Fragments arranged by size:
3463 1210 1115
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5788
BfrBI ATG'CAT
Cuts at: 2547 2547
Size: 5788
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4399 136
Size: 107 122 71 631 605 2727 1525
Fragments arranged by size:
2727 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2658 5783 2658
Size: 3125 2663
BlpI GC'TnA_GC
Cuts at: 3205 3205
Size: 5788
Bme1580I G_kGCm'C
Cuts at: 1790 2409 3849 5005 1790
Size: 619 1440 1156 2573
Fragments arranged by size:
2573 1440 1156 619
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5788
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3519 4477 442
Size: 416 53 83 399 24 2102 958 1753
Fragments arranged by size:
2102 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5788
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2314 4448 1239
Size: 1075 2134 2579
Fragments arranged by size:
2579 2134 1075
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3913 4778 5076 3913
Size: 865 298 4625
Fragments arranged by size:
4625 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4451 882
Size: 617 2952 2219
Fragments arranged by size:
2952 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3035 3579 493
Size: 711 1831 544 2702
Fragments arranged by size:
2702 1831 711 544
BsaBI GATnn'nnATC
Cuts at: 5782 5782
Size: 5788
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3595 3977 4612 275
Size: 53 83 186 936 2062 382 635 1451
Fragments arranged by size:
2062 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4221 4619 4893 5040 5451 4221
Size: 398 274 147 411 4558
Fragments arranged by size:
4558 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2484 2514 1178
Size: 30 165 30 1081 30 4452
Fragments arranged by size:
4452 1081 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2040 2492 1186
Size: 854 452 4482
Fragments arranged by size:
4482 854 452
BseYI C'CCAG_C
Cuts at: 4991 5487 4991
Size: 496 5292
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2858 1163
Size: 573 1122 4093
Fragments arranged by size:
4093 1122 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3238 3999 5027 1540
Size: 275 186 1237 761 1028 2301
Fragments arranged by size:
2301 1237 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2409 3849 3934 5005 2409
Size: 1440 85 1071 3192
Fragments arranged by size:
3192 1440 1071 85
BsmI GAATG_Cn'
Cuts at: 3299 3392 3299
Size: 93 5695
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2142 2409 3107 3849 3934 5005 1391
Size: 399 352 267 698 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 698 399 352 267 85
BspEI T'CCGG_A
Cuts at: 5451 5451
Size: 5788
BspHI T'CATG_A
Cuts at: 3677 5407 3677
Size: 1730 4058
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2345 2381 3143 844
Size: 1501 36 762 3489
Fragments arranged by size:
3489 1501 762 36
BsrBI CCG'CTC
Cuts at: 1394 1823 3675 1394
Size: 429 1852 3507
Fragments arranged by size:
3507 1852 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2286 2394 4283 4457 65
Size: 1183 1038 108 1889 174 1396
Fragments arranged by size:
1889 1396 1183 1038 174 108
BsrFI r'CCGG_y
Cuts at: 1700 1810 4432 4619 1700
Size: 110 2622 187 2869
Fragments arranged by size:
2869 2622 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5788
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5788
BssSI C'ACGA_G
Cuts at: 2141 3848 4860 2141
Size: 1707 1012 3069
Fragments arranged by size:
3069 1707 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5788
BstBI TT'CG_AA
Cuts at: 4592 4592
Size: 5788
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5788
BstYI r'GATC_y
Cuts at: 1972 2658 3118 3485 3870 3887 5339 5425 5437
Size: 686 460 367 385 17 1452 86 12
Cuts at: 5437 5783 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 686 460 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2052 2052
Size: 5788
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4297
Fragments arranged by size:
4297 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5049
Fragments arranged by size:
5049 683 56
BtsI GCAGTG_nn'
Cuts at: 3304 4098 4118 3304
Size: 794 20 4974
Fragments arranged by size:
4974 794 20
ClaI AT'CG_AT
Cuts at: 1554 2993 1554
Size: 1439 4349
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5753
DraI TTT'AAA
Cuts at: 3092 3092
Size: 5788
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4795 809
Size: 507 3479 1802
Fragments arranged by size:
3479 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2091 3235 4124
Size: 54 1425 50 275 18 261 1144 889
Cuts at: 4124 5753 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1144 889 275 261 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3235 1537
Size: 275 1423 4090
Fragments arranged by size:
4090 1423 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2586 2678 3718 1353
Size: 1233 92 1040 3423
Fragments arranged by size:
3423 1233 1040 92
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2025 4375 4749 4895 387
Size: 1064 138 436 2350 374 146 1280
Fragments arranged by size:
2350 1280 1064 436 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2314 2659 3042 3851 4448 5235 5503
Size: 393 682 345 383 809 597 787 268
Cuts at: 5503 1239
Size: 1524
Fragments arranged by size:
1524 809 787 682 597 393 383 345
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2314 2314
Size: 5788
EcoRI G'AATT_C
Cuts at: 3097 3475 3097
Size: 378 5410
FspI TGC'GCA
Cuts at: 4294 4294
Size: 5788
HaeII r_GCGC'y
Cuts at: 1678 4935 1678
Size: 3257 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4429 4461 4503 4535 1373
Size: 32 135 32 2857 32 42 32 2626
Fragments arranged by size:
2857 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3132 3378 669
Size: 988 281 1194 246 3079
Fragments arranged by size:
3079 1194 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2816 748
Size: 2068 3720
HpaI GTT'AAC
Cuts at: 3378 3378
Size: 5788
KpnI G_GTAC'C
Cuts at: 3113 3113
Size: 5788
MfeI C'AATT_G
Cuts at: 2902 3387 2902
Size: 485 5303
MluI A'CGCG_T
Cuts at: 5664 5664
Size: 5788
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4902 5086 1305
Size: 3597 184 2007
Fragments arranged by size:
3597 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3966
Fragments arranged by size:
3966 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2399 3746 4105 4264 518
Size: 648 1233 1347 359 159 2042
Fragments arranged by size:
2042 1347 1233 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3204 3881 5029 5274 1606
Size: 1598 677 1148 245 2120
Fragments arranged by size:
2120 1598 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4297
Fragments arranged by size:
4297 939 552
NdeI CA'TA_TG
Cuts at: 387 2350 2805 387
Size: 1963 455 3370
Fragments arranged by size:
3370 1963 455
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5788
NotI GC'GGCC_GC
Cuts at: 3235 3235
Size: 5788
NsiI A_TGCA'T
Cuts at: 2549 2549
Size: 5788
NspI r_CATG'y
Cuts at: 1132 1229 2447 2547 2607 2940 3146 4691 1132
Size: 97 1218 100 60 333 206 1545 2229
Fragments arranged by size:
2229 1545 1218 333 206 100 97 60
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5788
PciI A'CATG_T
Cuts at: 4687 4687
Size: 5788
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5788
PfoI T'CCnGG_A
Cuts at: 2835 2835
Size: 5788
PmeI GTTT'AAAC
Cuts at: 3092 3092
Size: 5788
PmlI CAC'GTG
Cuts at: 3035 3035
Size: 5788
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3857 3889 5370 5402 3857
Size: 32 1481 32 4243
Fragments arranged by size:
4243 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5788
PsiI TTA'TAA
Cuts at: 2633 3358 2633
Size: 725 5063
PstI C_TGCA'G
Cuts at: 830 3140 830
Size: 2310 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5788
SalI G'TCGA_C
Cuts at: 1936 3130 1936
Size: 1194 4594
SbfI CC_TGCA'GG
Cuts at: 3140 3140
Size: 5788
ScaI AGT'ACT
Cuts at: 4036 5748 4036
Size: 1712 4076
SfcI C'TryA_G
Cuts at: 826 1045 3136 4271 4952 5143 826
Size: 219 2091 1135 681 191 1471
Fragments arranged by size:
2091 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5788
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5788
SmaI CCC'GGG
Cuts at: 1872 3115 1872
Size: 1243 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3892 4793 5055 820
Size: 197 926 1949 901 262 1553
Fragments arranged by size:
1949 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3579 493
Size: 3086 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5788
SphI G_CATG'C
Cuts at: 1132 3146 1132
Size: 2014 3774
SspI AAT'ATT
Cuts at: 5 52 3712 5
Size: 47 3660 2081
Fragments arranged by size:
3660 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5788
StyI C'CwwG_G
Cuts at: 513 1065 2004 2160 3227 513
Size: 552 939 156 1067 3074
Fragments arranged by size:
3074 1067 939 552 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3815 3832 3985 4170 4615 325
Size: 1255 2235 17 153 185 445 1498
Fragments arranged by size:
2235 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2620 4034 5746
Size: 274 80 33 51 1443 643 1414 1712
Cuts at: 5746 96
Size: 138
Fragments arranged by size:
1712 1443 1414 643 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2484 2516 5748 5780 2484
Size: 32 3232 32 2492
Fragments arranged by size:
3232 2492 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5788
XbaI T'CTAG_A
Cuts at: 3124 3124
Size: 5788
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5788
XmaI C'CCGG_G
Cuts at: 1870 3113 1870
Size: 1243 4545
XmnI GAAnn'nnTTC
Cuts at: 3917 3917
Size: 5788
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3596 276
Size: 53 83 186 936 2062 2468
Fragments arranged by size:
2468 2062 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI