Restriction map for
pFN21AB8808
(Circular) (Six-base) MAPSORT of: pFN21AB8808.seq Check: 733 from: 1 to: 5770
pFN21AB8808 5770 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3580 278
Size: 53 83 186 936 2044 2468
Fragments arranged by size:
2468 2044 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2220 3057 3113 1937
Size: 283 837 56 4594
Fragments arranged by size:
4594 837 283 56
Acc65I G'GTAC_C
Cuts at: 2397 3091 2397
Size: 694 5076
AclI AA'CG_TT
Cuts at: 1563 3897 4270 1563
Size: 2334 373 3063
Fragments arranged by size:
3063 2334 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2553 2699 3833 5217 5485 1632
Size: 921 146 1134 1384 268 1917
Fragments arranged by size:
1917 1384 1134 921 268 146
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5573
AflIII A'CryG_T
Cuts at: 1203 4669 5646 1203
Size: 3466 977 1327
Fragments arranged by size:
3466 1327 977
AgeI A'CCGG_T
Cuts at: 4601 4601
Size: 5770
AhdI GACnn_n'nnGTC
Cuts at: 4499 4499
Size: 5770
AleI CACnn'nnGTG
Cuts at: 1166 2883 1166
Size: 1717 4053
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5770
AlwNI CAG_nnn'CTG
Cuts at: 3513 5085 3513
Size: 1572 4198
ApaLI G'TGCA_C
Cuts at: 3827 4983 3827
Size: 1156 4614
ApoI r'AATT_y
Cuts at: 1493 3079 3305 3457 5566 1493
Size: 1586 226 152 2109 1697
Fragments arranged by size:
2109 1697 1586 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4324 160
Size: 872 3292 1606
Fragments arranged by size:
3292 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5770
AvaI C'yCGr_G
Cuts at: 1870 1943 3095 1870
Size: 73 1152 4545
Fragments arranged by size:
4545 1152 73
AvrII C'CTAG_G
Cuts at: 2980 2996 2980
Size: 16 5754
BamHI G'GATC_C
Cuts at: 3100 3467 3100
Size: 367 5403
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2397 3091 4546 618
Size: 325 217 627 610 694 1455 1842
Fragments arranged by size:
1842 1455 694 627 610 325 217
BanII G_rGCy'C
Cuts at: 1391 3089 1391
Size: 1698 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2144 2251 2992 928
Size: 971 245 107 741 3706
Fragments arranged by size:
3706 971 741 245 107
BbvCI CC'TCA_GC
Cuts at: 2201 2201
Size: 5770
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3961 3995 1903
Size: 34 2024 34 3678
Fragments arranged by size:
3678 2024 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3662 4872 3662
Size: 1210 4560
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5770
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2492 4381 136
Size: 107 122 71 631 605 820 1889 1525
Fragments arranged by size:
1889 1525 820 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2589 5765 2589
Size: 3176 2594
BlpI GC'TnA_GC
Cuts at: 3187 3187
Size: 5770
Bme1580I G_kGCm'C
Cuts at: 1790 2456 3831 4987 1790
Size: 666 1375 1156 2573
Fragments arranged by size:
2573 1375 1156 666
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5770
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3501 4459 442
Size: 416 53 83 399 24 2084 958 1753
Fragments arranged by size:
2084 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5770
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3034 4430 1239
Size: 1795 1396 2579
Fragments arranged by size:
2579 1795 1396
Bpu10I CC'TnA_GC
Cuts at: 2201 2201
Size: 5770
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2053 3895 4760 5058 2053
Size: 1842 865 298 2765
Fragments arranged by size:
2765 1842 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4433 882
Size: 617 2934 2219
Fragments arranged by size:
2934 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3561 493
Size: 711 2357 2702
Fragments arranged by size:
2702 2357 711
BsaBI GATnn'nnATC
Cuts at: 5764 5764
Size: 5770
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3577 3959 4594 275
Size: 53 83 186 936 2044 382 635 1451
Fragments arranged by size:
2044 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4203 4601 4875 5022 5433 4203
Size: 398 274 147 411 4540
Fragments arranged by size:
4540 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5545
Fragments arranged by size:
5545 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2218 3028 1186
Size: 1032 810 3928
Fragments arranged by size:
3928 1032 810
BseYI C'CCAG_C
Cuts at: 4973 5469 4973
Size: 496 5274
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2654 1163
Size: 573 918 4279
Fragments arranged by size:
4279 918 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3220 3981 5009 1540
Size: 275 186 1219 761 1028 2301
Fragments arranged by size:
2301 1219 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2727 3831 3916 4987 2727
Size: 1104 85 1071 3510
Fragments arranged by size:
3510 1104 1071 85
BsmI GAATG_Cn'
Cuts at: 3281 3374 3281
Size: 93 5677
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2456 2727 3089 3831 3916 4987 1391
Size: 399 666 271 362 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 666 399 362 271 85
BspEI T'CCGG_A
Cuts at: 5433 5433
Size: 5770
BspHI T'CATG_A
Cuts at: 2382 3659 5389 2382
Size: 1277 1730 2763
Fragments arranged by size:
2763 1730 1277
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2256 3125 844
Size: 1412 869 3489
Fragments arranged by size:
3489 1412 869
BsrBI CCG'CTC
Cuts at: 1394 1823 3657 1394
Size: 429 1834 3507
Fragments arranged by size:
3507 1834 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2504 2736 4265 4439 65
Size: 1183 1256 232 1529 174 1396
Fragments arranged by size:
1529 1396 1256 1183 232 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4414 4601 1700
Size: 110 2604 187 2869
Fragments arranged by size:
2869 2604 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5770
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5770
BssSI C'ACGA_G
Cuts at: 3830 4842 3830
Size: 1012 4758
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2800 794
Size: 2006 3764
BstBI TT'CG_AA
Cuts at: 4574 4574
Size: 5770
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5770
BstXI CCAn_nnnn'nTGG
Cuts at: 2719 2719
Size: 5770
BstYI r'GATC_y
Cuts at: 1972 2589 3100 3467 3852 3869 5321 5407 5419
Size: 617 511 367 385 17 1452 86 12
Cuts at: 5419 5765 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 617 511 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 2221 2221
Size: 5770
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4279
Fragments arranged by size:
4279 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5031
Fragments arranged by size:
5031 683 56
BtsI GCAGTG_nn'
Cuts at: 2878 3286 4080 4100 2878
Size: 408 794 20 4548
Fragments arranged by size:
4548 794 408 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5770
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5735
DraI TTT'AAA
Cuts at: 3074 3074
Size: 5770
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4777 809
Size: 507 3461 1802
Fragments arranged by size:
3461 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3217 4106 5735
Size: 54 1425 50 275 18 1387 889 1629
Cuts at: 5735 8
Size: 43
Fragments arranged by size:
1629 1425 1387 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3217 1537
Size: 275 1405 4090
Fragments arranged by size:
4090 1405 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2675 3700 1353
Size: 1322 1025 3423
Fragments arranged by size:
3423 1322 1025
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4357 4731 4877 387
Size: 1064 138 2768 374 146 1280
Fragments arranged by size:
2768 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2553 2699 3034 3833 4430 5217 5485
Size: 393 921 146 335 799 597 787 268
Cuts at: 5485 1239
Size: 1524
Fragments arranged by size:
1524 921 799 787 597 393 335 268
146
EcoO109I rG'GnC_Cy
Cuts at: 2758 2758
Size: 5770
EcoRI G'AATT_C
Cuts at: 3079 3457 3079
Size: 378 5392
EcoRV GAT'ATC
Cuts at: 2062 2062
Size: 5770
FspI TGC'GCA
Cuts at: 4276 4276
Size: 5770
HaeII r_GCGC'y
Cuts at: 1678 4917 1678
Size: 3239 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2051 2083 2269 2301 4411
Size: 32 135 32 479 32 186 32 2110
Cuts at: 4411 4443 4485 4517 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2110 479 186 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3114 3360 669
Size: 988 281 1176 246 3079
Fragments arranged by size:
3079 1176 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5770
HpaI GTT'AAC
Cuts at: 3360 3360
Size: 5770
KpnI G_GTAC'C
Cuts at: 2401 3095 2401
Size: 694 5076
MfeI C'AATT_G
Cuts at: 3369 3369
Size: 5770
MluI A'CGCG_T
Cuts at: 5646 5646
Size: 5770
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4884 5068 1305
Size: 3579 184 2007
Fragments arranged by size:
3579 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3948
Fragments arranged by size:
3948 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2381 2387 2645 2883 3728 4087 4246
Size: 648 1215 6 258 238 845 359 159
Cuts at: 4246 518
Size: 2042
Fragments arranged by size:
2042 1215 845 648 359 258 238 159
6
MspA1I CmG'CkG
Cuts at: 1606 2677 2836 3186 3863 5011 5256 1606
Size: 1071 159 350 677 1148 245 2120
Fragments arranged by size:
2120 1148 1071 677 350 245 159
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4279
Fragments arranged by size:
4279 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5770
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5770
NotI GC'GGCC_GC
Cuts at: 3217 3217
Size: 5770
NspI r_CATG'y
Cuts at: 1132 1229 2368 3128 4673 1132
Size: 97 1139 760 1545 2229
Fragments arranged by size:
2229 1545 1139 760 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5770
PciI A'CATG_T
Cuts at: 4669 4669
Size: 5770
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5770
PmeI GTTT'AAAC
Cuts at: 3074 3074
Size: 5770
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3839 3871 5352 5384 3839
Size: 32 1481 32 4225
Fragments arranged by size:
4225 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2758 2758
Size: 5770
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5770
PsiI TTA'TAA
Cuts at: 3340 3340
Size: 5770
PstI C_TGCA'G
Cuts at: 830 3122 830
Size: 2292 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5770
PvuII CAG'CTG
Cuts at: 2677 2836 2677
Size: 159 5611
SalI G'TCGA_C
Cuts at: 1936 3112 1936
Size: 1176 4594
SanDI GG'GwC_CC
Cuts at: 2758 2758
Size: 5770
SbfI CC_TGCA'GG
Cuts at: 3122 3122
Size: 5770
ScaI AGT'ACT
Cuts at: 4018 5730 4018
Size: 1712 4058
SfcI C'TryA_G
Cuts at: 826 1045 2356 2546 3118 4253 4934 5125 826
Size: 219 1311 190 572 1135 681 191 1471
Fragments arranged by size:
1471 1311 1135 681 572 219 191 190
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5770
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5770
SmaI CCC'GGG
Cuts at: 1872 3097 1872
Size: 1225 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2068 3874 4775 5037 820
Size: 197 926 125 1806 901 262 1553
Fragments arranged by size:
1806 1553 926 901 262 197 125
SnaBI TAC'GTA
Cuts at: 493 3561 493
Size: 3068 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5770
SphI G_CATG'C
Cuts at: 1132 3128 1132
Size: 1996 3774
SspI AAT'ATT
Cuts at: 5 52 2946 3694 5
Size: 47 2894 748 2081
Fragments arranged by size:
2894 2081 748 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5770
StyI C'CwwG_G
Cuts at: 513 1065 2004 2706 2980 2996 3209 513
Size: 552 939 702 274 16 213 3074
Fragments arranged by size:
3074 939 702 552 274 213 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2057 2290 2864 3797 3814 3967 4152
Size: 1255 477 233 574 933 17 153 185
Cuts at: 4152 4597 325
Size: 445 1498
Fragments arranged by size:
1498 1255 933 574 477 445 233 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4016 5728 96
Size: 274 80 33 51 1443 2039 1712 138
Fragments arranged by size:
2039 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5730 5762 5730
Size: 32 5738
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5770
XbaI T'CTAG_A
Cuts at: 3106 3106
Size: 5770
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2303 2303
Size: 5770
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5770
XmaI C'CCGG_G
Cuts at: 1870 3095 1870
Size: 1225 4545
XmnI GAAnn'nnTTC
Cuts at: 2091 2591 2946 3899 2091
Size: 500 355 953 3962
Fragments arranged by size:
3962 953 500 355
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3578 276
Size: 53 83 186 936 2044 2468
Fragments arranged by size:
2468 2044 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BfrBI
BplI BsiWI BsmBI Bsu36I DraIII EcoICRI EcoNI FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SapI SexAI SfiI SfoI SrfI SwaI