Restriction map for
pFN21AB8807
(Circular) (Six-base) MAPSORT of: pFN21AB8807.seq Check: 2833 from: 1 to: 5770
pFN21AB8807 5770 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3580 278
Size: 53 83 186 936 2044 2468
Fragments arranged by size:
2468 2044 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3113 1937
Size: 1176 4594
Acc65I G'GTAC_C
Cuts at: 2039 2397 3091 2039
Size: 358 694 4718
Fragments arranged by size:
4718 694 358
AclI AA'CG_TT
Cuts at: 1563 3897 4270 1563
Size: 2334 373 3063
Fragments arranged by size:
3063 2334 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2547 3833 5217 5485 1632
Size: 915 1286 1384 268 1917
Fragments arranged by size:
1917 1384 1286 915 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5573
AflIII A'CryG_T
Cuts at: 1203 4669 5646 1203
Size: 3466 977 1327
Fragments arranged by size:
3466 1327 977
AgeI A'CCGG_T
Cuts at: 4601 4601
Size: 5770
AhdI GACnn_n'nnGTC
Cuts at: 4499 4499
Size: 5770
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5770
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5770
AlwNI CAG_nnn'CTG
Cuts at: 3513 5085 3513
Size: 1572 4198
ApaLI G'TGCA_C
Cuts at: 3827 4983 3827
Size: 1156 4614
ApoI r'AATT_y
Cuts at: 1493 2507 3079 3305 3457 5566 1493
Size: 1014 572 226 152 2109 1697
Fragments arranged by size:
2109 1697 1014 572 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4324 160
Size: 872 3292 1606
Fragments arranged by size:
3292 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5770
AvaI C'yCGr_G
Cuts at: 1870 1943 3095 1870
Size: 73 1152 4545
Fragments arranged by size:
4545 1152 73
BamHI G'GATC_C
Cuts at: 2274 3100 3467 2274
Size: 826 367 4577
Fragments arranged by size:
4577 826 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2039 2397 2688 3091 4546
Size: 325 217 627 252 358 291 403 1455
Cuts at: 4546 618
Size: 1842
Fragments arranged by size:
1842 1455 627 403 358 325 291 252
217
BanII G_rGCy'C
Cuts at: 1391 2437 3089 1391
Size: 1046 652 4072
Fragments arranged by size:
4072 1046 652
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4799
BbvCI CC'TCA_GC
Cuts at: 2684 2684
Size: 5770
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3961 3995 1903
Size: 34 2024 34 3678
Fragments arranged by size:
3678 2024 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2566 3662 4872 2566
Size: 1096 1210 3464
Fragments arranged by size:
3464 1210 1096
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5770
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2154 4381 136
Size: 107 122 71 631 605 482 2227 1525
Fragments arranged by size:
2227 1525 631 605 482 122 107 71
BglII A'GATC_T
Cuts at: 2364 5765 2364
Size: 3401 2369
BlpI GC'TnA_GC
Cuts at: 3187 3187
Size: 5770
Bme1580I G_kGCm'C
Cuts at: 1790 2118 2693 3831 4987 1790
Size: 328 575 1138 1156 2573
Fragments arranged by size:
2573 1156 1138 575 328
BmgBI CAC'GTC
Cuts at: 1318 2062 1318
Size: 744 5026
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2101 2308 2688
Size: 416 53 83 399 24 684 207 380
Cuts at: 2688 3501 4459 442
Size: 813 958 1753
Fragments arranged by size:
1753 958 813 684 416 399 380 207
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2495 1056
Size: 1439 4331
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2608 4430 1239
Size: 1369 1822 2579
Fragments arranged by size:
2579 1822 1369
Bpu10I CC'TnA_GC
Cuts at: 2684 2684
Size: 5770
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3895 4760 5058 3895
Size: 865 298 4607
Fragments arranged by size:
4607 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2024 4433 882
Size: 617 525 2409 2219
Fragments arranged by size:
2409 2219 617 525
BsaAI yAC'GTr
Cuts at: 493 1204 3561 493
Size: 711 2357 2702
Fragments arranged by size:
2702 2357 711
BsaBI GATnn'nnATC
Cuts at: 5764 5764
Size: 5770
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3577 3959 4594 275
Size: 53 83 186 936 2044 382 635 1451
Fragments arranged by size:
2044 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2928 4203 4601 4875 5022 5433 2928
Size: 1275 398 274 147 411 3265
Fragments arranged by size:
3265 1275 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5545
Fragments arranged by size:
5545 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2318 1186
Size: 1132 4638
BseYI C'CCAG_C
Cuts at: 4973 5469 4973
Size: 496 5274
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5197
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3220 3981 5009 1540
Size: 275 186 1219 761 1028 2301
Fragments arranged by size:
2301 1219 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2098 2560 3831 3916 4987 2098
Size: 462 1271 85 1071 2881
Fragments arranged by size:
2881 1271 1071 462 85
BsmI GAATG_Cn'
Cuts at: 3281 3374 3281
Size: 93 5677
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2098 2118 2437 2560 2693 3089 3831
Size: 399 308 20 319 123 133 396 742
Cuts at: 3831 3916 4987 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 396 319 308 133
123 85 20
BspEI T'CCGG_A
Cuts at: 2928 5433 2928
Size: 2505 3265
BspHI T'CATG_A
Cuts at: 3659 5389 3659
Size: 1730 4040
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2229 2253 2946 3125 844
Size: 1385 24 693 179 3489
Fragments arranged by size:
3489 1385 693 179 24
BsrBI CCG'CTC
Cuts at: 1394 1823 2827 3657 1394
Size: 429 1004 830 3507
Fragments arranged by size:
3507 1004 830 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2164 4265 4439 65
Size: 1183 916 2101 174 1396
Fragments arranged by size:
2101 1396 1183 916 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4414 4601 1700
Size: 110 2604 187 2869
Fragments arranged by size:
2869 2604 187 110
BsrGI T'GTAC_A
Cuts at: 96 2355 96
Size: 2259 3511
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5770
BssSI C'ACGA_G
Cuts at: 3830 4842 3830
Size: 1012 4758
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5770
BstBI TT'CG_AA
Cuts at: 4574 4574
Size: 5770
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5770
BstYI r'GATC_y
Cuts at: 1972 2274 2364 3100 3467 3852 3869 5321 5407
Size: 302 90 736 367 385 17 1452 86
Cuts at: 5407 5419 5765 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 736 385 367 346 302 90
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2311 513
Size: 552 1246 3972
Fragments arranged by size:
3972 1246 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5031
Fragments arranged by size:
5031 683 56
BtsI GCAGTG_nn'
Cuts at: 2493 2560 2881 3286 4080 4100 2493
Size: 67 321 405 794 20 4163
Fragments arranged by size:
4163 794 405 321 67 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5770
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5735
DraI TTT'AAA
Cuts at: 3074 3074
Size: 5770
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4777 809
Size: 507 3461 1802
Fragments arranged by size:
3461 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2232 2308 2403
Size: 54 1425 50 275 18 402 76 95
Cuts at: 2403 2649 3217 4106 5735 8
Size: 246 568 889 1629 43
Fragments arranged by size:
1629 1425 889 568 402 275 246 95
76 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3217 1537
Size: 275 1405 4090
Fragments arranged by size:
4090 1405 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2255 2505 2523 2535 2794 3700 1353
Size: 902 250 18 12 259 906 3423
Fragments arranged by size:
3423 906 902 259 250 18 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4357 4731 4877 387
Size: 1064 138 2768 374 146 1280
Fragments arranged by size:
2768 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2547 2608 3833 4430 5217 5485 1239
Size: 393 915 61 1225 597 787 268 1524
Fragments arranged by size:
1524 1225 915 787 597 393 268 61
EcoO109I rG'GnC_Cy
Cuts at: 2479 2479
Size: 5770
EcoRI G'AATT_C
Cuts at: 3079 3457 3079
Size: 378 5392
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2564 2564
Size: 5770
FspI TGC'GCA
Cuts at: 4276 4276
Size: 5770
HaeII r_GCGC'y
Cuts at: 1678 4917 1678
Size: 3239 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2821 2853 4411 4443 4485
Size: 32 135 32 1249 32 1558 32 42
Cuts at: 4485 4517 1373
Size: 32 2626
Fragments arranged by size:
2626 1558 1249 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3114 3360 669
Size: 988 281 1176 246 3079
Fragments arranged by size:
3079 1176 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5770
HpaI GTT'AAC
Cuts at: 3360 3360
Size: 5770
KpnI G_GTAC'C
Cuts at: 2043 2401 3095 2043
Size: 358 694 4718
Fragments arranged by size:
4718 694 358
MfeI C'AATT_G
Cuts at: 3369 3369
Size: 5770
MluI A'CGCG_T
Cuts at: 5646 5646
Size: 5770
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2896 4884 5068 1305
Size: 1591 1988 184 2007
Fragments arranged by size:
2007 1988 1591 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2234 2310 2405 2651 10
Size: 54 1425 343 402 76 95 246 3129
Fragments arranged by size:
3129 1425 402 343 246 95 76 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2741 2855 3728 4087 4246 518
Size: 648 1575 114 873 359 159 2042
Fragments arranged by size:
2042 1575 873 648 359 159 114
MspA1I CmG'CkG
Cuts at: 1606 2122 3186 3863 5011 5256 1606
Size: 516 1064 677 1148 245 2120
Fragments arranged by size:
2120 1148 1064 677 516 245
NcoI C'CATG_G
Cuts at: 513 1065 2311 513
Size: 552 1246 3972
Fragments arranged by size:
3972 1246 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5770
NheI G'CTAG_C
Cuts at: 1052 2491 1052
Size: 1439 4331
NotI GC'GGCC_GC
Cuts at: 3217 3217
Size: 5770
NspI r_CATG'y
Cuts at: 1132 1229 2320 3128 4673 1132
Size: 97 1091 808 1545 2229
Fragments arranged by size:
2229 1545 1091 808 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5770
PciI A'CATG_T
Cuts at: 4669 4669
Size: 5770
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5770
PmeI GTTT'AAAC
Cuts at: 3074 3074
Size: 5770
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3839 3871 5352 5384 3839
Size: 32 1481 32 4225
Fragments arranged by size:
4225 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5770
PsiI TTA'TAA
Cuts at: 3340 3340
Size: 5770
PstI C_TGCA'G
Cuts at: 830 3122 830
Size: 2292 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5770
SalI G'TCGA_C
Cuts at: 1936 3112 1936
Size: 1176 4594
SapI GCTCTTCn'nnn_
Cuts at: 2255 2505 2255
Size: 250 5520
SbfI CC_TGCA'GG
Cuts at: 3122 3122
Size: 5770
ScaI AGT'ACT
Cuts at: 4018 5730 4018
Size: 1712 4058
SexAI A'CCwGG_T
Cuts at: 2844 2844
Size: 5770
SfcI C'TryA_G
Cuts at: 826 1045 3118 4253 4934 5125 826
Size: 219 2073 1135 681 191 1471
Fragments arranged by size:
2073 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5770
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5770
SmaI CCC'GGG
Cuts at: 1872 3097 1872
Size: 1225 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3874 4775 5037 820
Size: 197 926 1931 901 262 1553
Fragments arranged by size:
1931 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3561 493
Size: 3068 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5770
SphI G_CATG'C
Cuts at: 1132 3128 1132
Size: 1996 3774
SspI AAT'ATT
Cuts at: 5 52 3694 5
Size: 47 3642 2081
Fragments arranged by size:
3642 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5770
StyI C'CwwG_G
Cuts at: 513 1065 2311 3209 513
Size: 552 1246 898 3074
Fragments arranged by size:
3074 1246 898 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3797 3814 3967 4152 4597 325
Size: 1255 2217 17 153 185 445 1498
Fragments arranged by size:
2217 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2124 2355 2706
Size: 274 80 33 51 1443 147 231 351
Cuts at: 2706 4016 5728 96
Size: 1310 1712 138
Fragments arranged by size:
1712 1443 1310 351 274 231 147 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5730 5762 5730
Size: 32 5738
Tth111I GACn'n_nGTC
Cuts at: 1316 2846 1316
Size: 1530 4240
XbaI T'CTAG_A
Cuts at: 3106 3106
Size: 5770
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2098 2098
Size: 5770
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5770
XmaI C'CCGG_G
Cuts at: 1870 3095 1870
Size: 1225 4545
XmnI GAAnn'nnTTC
Cuts at: 3899 3899
Size: 5770
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3578 276
Size: 53 83 186 936 2044 2468
Fragments arranged by size:
2468 2044 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SfiI
SfoI SrfI SwaI