Restriction map for
pFN21AB8806
(Circular) (Six-base) MAPSORT of: pFN21AB8806.seq Check: 4616 from: 1 to: 5770
pFN21AB8806 5770 bp
With 182 enzymes: SgfI *
October 20, 2009 10:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2558 2558
Size: 5770
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3580 278
Size: 53 83 186 936 2044 2468
Fragments arranged by size:
2468 2044 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3113 1937
Size: 1176 4594
Acc65I G'GTAC_C
Cuts at: 2397 3091 2397
Size: 694 5076
AclI AA'CG_TT
Cuts at: 1563 3897 4270 1563
Size: 2334 373 3063
Fragments arranged by size:
3063 2334 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3833 5217 5485 1632
Size: 2201 1384 268 1917
Fragments arranged by size:
2201 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5573
AflIII A'CryG_T
Cuts at: 1203 4669 5646 1203
Size: 3466 977 1327
Fragments arranged by size:
3466 1327 977
AgeI A'CCGG_T
Cuts at: 4601 4601
Size: 5770
AhdI GACnn_n'nnGTC
Cuts at: 4499 4499
Size: 5770
AleI CACnn'nnGTG
Cuts at: 1166 2886 2958 1166
Size: 1720 72 3978
Fragments arranged by size:
3978 1720 72
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2089 1184
Size: 905 4865
AlwNI CAG_nnn'CTG
Cuts at: 2605 3513 5085 2605
Size: 908 1572 3290
Fragments arranged by size:
3290 1572 908
ApaI G_GGCC'C
Cuts at: 2107 2482 2107
Size: 375 5395
ApaLI G'TGCA_C
Cuts at: 2969 3827 4983 2969
Size: 858 1156 3756
Fragments arranged by size:
3756 1156 858
ApoI r'AATT_y
Cuts at: 1493 2534 3079 3305 3457 5566 1493
Size: 1041 545 226 152 2109 1697
Fragments arranged by size:
2109 1697 1041 545 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4324 160
Size: 872 3292 1606
Fragments arranged by size:
3292 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5770
AvaI C'yCGr_G
Cuts at: 1870 1943 3095 1870
Size: 73 1152 4545
Fragments arranged by size:
4545 1152 73
BamHI G'GATC_C
Cuts at: 3100 3467 3100
Size: 367 5403
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2397 3091 4546 618
Size: 325 217 627 610 694 1455 1842
Fragments arranged by size:
1842 1455 694 627 610 325 217
BanII G_rGCy'C
Cuts at: 1391 2107 2482 3089 1391
Size: 716 375 607 4072
Fragments arranged by size:
4072 716 607 375
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4799
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3961 3995 1903
Size: 34 2024 34 3678
Fragments arranged by size:
3678 2024 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3662 4872 3662
Size: 1210 4560
BclI T'GATC_A
Cuts at: 1548 2649 2739 1548
Size: 1101 90 4579
Fragments arranged by size:
4579 1101 90
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2102 2432 2492
Size: 107 122 71 631 605 430 330 60
Cuts at: 2492 4381 136
Size: 1889 1525
Fragments arranged by size:
1889 1525 631 605 430 330 122 107
71 60
BglII A'GATC_T
Cuts at: 2364 5765 2364
Size: 3401 2369
BlpI GC'TnA_GC
Cuts at: 3187 3187
Size: 5770
Bme1580I G_kGCm'C
Cuts at: 1790 2107 2437 2482 2973 3831 4987 1790
Size: 317 330 45 491 858 1156 2573
Fragments arranged by size:
2573 1156 858 491 330 317 45
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5770
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2308 2891 3501
Size: 416 53 83 399 24 891 583 610
Cuts at: 3501 4459 442
Size: 958 1753
Fragments arranged by size:
1753 958 891 610 583 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5770
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3032 4430 1239
Size: 1793 1398 2579
Fragments arranged by size:
2579 1793 1398
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3895 4760 5058 3895
Size: 865 298 4607
Fragments arranged by size:
4607 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4433 882
Size: 617 2934 2219
Fragments arranged by size:
2934 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3561 493
Size: 711 2357 2702
Fragments arranged by size:
2702 2357 711
BsaBI GATnn'nnATC
Cuts at: 5764 5764
Size: 5770
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3577 3959 4594 275
Size: 53 83 186 936 2044 382 635 1451
Fragments arranged by size:
2044 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2928 4203 4601 4875 5022 5433 2928
Size: 1275 398 274 147 411 3265
Fragments arranged by size:
3265 1275 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5545
Fragments arranged by size:
5545 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5770
BseYI C'CCAG_C
Cuts at: 3062 4973 5469 3062
Size: 1911 496 3363
Fragments arranged by size:
3363 1911 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5197
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3220 3981 5009 1540
Size: 275 186 1219 761 1028 2301
Fragments arranged by size:
2301 1219 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2973 3831 3916 4987 2973
Size: 858 85 1071 3756
Fragments arranged by size:
3756 1071 858 85
BsmI GAATG_Cn'
Cuts at: 3281 3374 3281
Size: 93 5677
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2107 2437 2482 2973 3089 3831 3916
Size: 399 317 330 45 491 116 742 85
Cuts at: 3916 4987 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 491 399 330 317 116
85 45
BspEI T'CCGG_A
Cuts at: 2928 5433 2928
Size: 2505 3265
BspHI T'CATG_A
Cuts at: 2646 2736 3659 5389 2646
Size: 90 923 1730 3027
Fragments arranged by size:
3027 1730 923 90
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2253 2558 2946 3125 844
Size: 1409 305 388 179 3489
Fragments arranged by size:
3489 1409 388 305 179
BsrBI CCG'CTC
Cuts at: 1394 1823 3657 1394
Size: 429 1834 3507
Fragments arranged by size:
3507 1834 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4265 4439 65
Size: 1183 3017 174 1396
Fragments arranged by size:
3017 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4414 4601 1700
Size: 110 2604 187 2869
Fragments arranged by size:
2869 2604 187 110
BsrGI T'GTAC_A
Cuts at: 96 2355 96
Size: 2259 3511
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5770
BssSI C'ACGA_G
Cuts at: 3830 4842 3830
Size: 1012 4758
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5770
BstBI TT'CG_AA
Cuts at: 4574 4574
Size: 5770
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5770
BstYI r'GATC_y
Cuts at: 1972 2325 2364 3100 3467 3852 3869 5321 5407
Size: 353 39 736 367 385 17 1452 86
Cuts at: 5407 5419 5765 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 736 385 367 353 346 86
39 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3000 513
Size: 552 939 996 3283
Fragments arranged by size:
3283 996 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5031
Fragments arranged by size:
5031 683 56
BtsI GCAGTG_nn'
Cuts at: 2881 3286 4080 4100 2881
Size: 405 794 20 4551
Fragments arranged by size:
4551 794 405 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5770
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2032 2067 529
Size: 35 1468 35 4232
Fragments arranged by size:
4232 1468 35 35
DraI TTT'AAA
Cuts at: 3074 3074
Size: 5770
DraIII CAC_nnn'GTG
Cuts at: 2419 2419
Size: 5770
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4777 809
Size: 507 3461 1802
Fragments arranged by size:
3461 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2232 2403 2484
Size: 54 1425 50 275 18 402 171 81
Cuts at: 2484 3217 4106 5735 8
Size: 733 889 1629 43
Fragments arranged by size:
1629 1425 889 733 402 275 171 81
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3217 1537
Size: 275 1405 4090
Fragments arranged by size:
4090 1405 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2255 3700 1353
Size: 902 1445 3423
Fragments arranged by size:
3423 1445 902
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4357 4731 4877 387
Size: 1064 138 2768 374 146 1280
Fragments arranged by size:
2768 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3032 3833 4430 5217 5485 1239
Size: 393 1400 801 597 787 268 1524
Fragments arranged by size:
1524 1400 801 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2094 2103 2479 2094
Size: 9 376 5385
Fragments arranged by size:
5385 376 9
EcoRI G'AATT_C
Cuts at: 2534 3079 3457 2534
Size: 545 378 4847
Fragments arranged by size:
4847 545 378
FspI TGC'GCA
Cuts at: 4276 4276
Size: 5770
HaeII r_GCGC'y
Cuts at: 1678 4917 1678
Size: 3239 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2690 2722 4411 4443 4485
Size: 32 135 32 1118 32 1689 32 42
Cuts at: 4485 4517 1373
Size: 32 2626
Fragments arranged by size:
2626 1689 1118 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2893 3114 3360 669
Size: 988 281 955 221 246 3079
Fragments arranged by size:
3079 988 955 281 246 221
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5770
HpaI GTT'AAC
Cuts at: 3360 3360
Size: 5770
KpnI G_GTAC'C
Cuts at: 2401 3095 2401
Size: 694 5076
MfeI C'AATT_G
Cuts at: 3369 3369
Size: 5770
MluI A'CGCG_T
Cuts at: 5646 5646
Size: 5770
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2134 4884 5068 1305
Size: 829 2750 184 2007
Fragments arranged by size:
2750 2007 829 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2234 2405 2486 10
Size: 54 1425 343 402 171 81 3294
Fragments arranged by size:
3294 1425 402 343 171 81 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2165 2820 2886 2958 3728 4087 4246
Size: 648 999 655 66 72 770 359 159
Cuts at: 4246 518
Size: 2042
Fragments arranged by size:
2042 999 770 655 648 359 159 72
66
MspA1I CmG'CkG
Cuts at: 1606 3186 3863 5011 5256 1606
Size: 1580 677 1148 245 2120
Fragments arranged by size:
2120 1580 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4279
Fragments arranged by size:
4279 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5770
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5770
NotI GC'GGCC_GC
Cuts at: 3217 3217
Size: 5770
NspI r_CATG'y
Cuts at: 1132 1229 3128 4673 1132
Size: 97 1899 1545 2229
Fragments arranged by size:
2229 1899 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5770
PciI A'CATG_T
Cuts at: 4669 4669
Size: 5770
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5770
PmeI GTTT'AAAC
Cuts at: 3074 3074
Size: 5770
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3839 3871 5352 5384 3839
Size: 32 1481 32 4225
Fragments arranged by size:
4225 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5770
PsiI TTA'TAA
Cuts at: 2672 3340 2672
Size: 668 5102
PspOMI G'GGCC_C
Cuts at: 2103 2478 2103
Size: 375 5395
PstI C_TGCA'G
Cuts at: 830 2555 3122 830
Size: 1725 567 3478
Fragments arranged by size:
3478 1725 567
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5770
RsrII CG'GwC_CG
Cuts at: 2439 2439
Size: 5770
SalI G'TCGA_C
Cuts at: 1936 3112 1936
Size: 1176 4594
SapI GCTCTTCn'nnn_
Cuts at: 2255 2255
Size: 5770
SbfI CC_TGCA'GG
Cuts at: 3122 3122
Size: 5770
ScaI AGT'ACT
Cuts at: 4018 5730 4018
Size: 1712 4058
SexAI A'CCwGG_T
Cuts at: 2973 2973
Size: 5770
SfcI C'TryA_G
Cuts at: 826 1045 2551 3118 4253 4934 5125 826
Size: 219 1506 567 1135 681 191 1471
Fragments arranged by size:
1506 1471 1135 681 567 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2102 2102
Size: 5770
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5770
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5770
SmaI CCC'GGG
Cuts at: 1872 3097 1872
Size: 1225 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3874 4775 5037 820
Size: 197 926 1931 901 262 1553
Fragments arranged by size:
1931 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3561 493
Size: 3068 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5770
SphI G_CATG'C
Cuts at: 1132 3128 1132
Size: 1996 3774
SspI AAT'ATT
Cuts at: 5 52 3694 5
Size: 47 3642 2081
Fragments arranged by size:
3642 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5770
StyI C'CwwG_G
Cuts at: 513 1065 2004 2130 3209 513
Size: 552 939 126 1079 3074
Fragments arranged by size:
3074 1079 939 552 126
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2828 3797 3814 3967 4152 4597 325
Size: 1255 1248 969 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1248 969 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2124 2355 4016
Size: 274 80 33 51 1443 147 231 1661
Cuts at: 4016 5728 96
Size: 1712 138
Fragments arranged by size:
1712 1661 1443 274 231 147 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5730 5762 5730
Size: 32 5738
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5770
XbaI T'CTAG_A
Cuts at: 3106 3106
Size: 5770
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5770
XmaI C'CCGG_G
Cuts at: 1870 3095 1870
Size: 1225 4545
XmnI GAAnn'nnTTC
Cuts at: 3899 3899
Size: 5770
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3578 276
Size: 53 83 186 936 2044 2468
Fragments arranged by size:
2468 2044 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
RsrII SalI SapI SbfI ScaI SexAI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI
PsrI PvuII SacI SacII SanDI SfoI SrfI SwaI
XcmI