Restriction map for
pFN21AB8803
(Circular) (Six-base) MAPSORT of: pFN21AB8803.seq Check: 7827 from: 1 to: 5752
pFN21AB8803 5752 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3562 278
Size: 53 83 186 936 2026 2468
Fragments arranged by size:
2468 2026 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3095 1937
Size: 1158 4594
Acc65I G'GTAC_C
Cuts at: 3073 3073
Size: 5752
AclI AA'CG_TT
Cuts at: 1563 3879 4252 1563
Size: 2316 373 3063
Fragments arranged by size:
3063 2316 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2513 2703 3815 5199 5467 1632
Size: 881 190 1112 1384 268 1917
Fragments arranged by size:
1917 1384 1112 881 268 190
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5555
AflIII A'CryG_T
Cuts at: 1203 4651 5628 1203
Size: 3448 977 1327
Fragments arranged by size:
3448 1327 977
AgeI A'CCGG_T
Cuts at: 4583 4583
Size: 5752
AhdI GACnn_n'nnGTC
Cuts at: 2555 4481 2555
Size: 1926 3826
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5752
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5752
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2745 2777 2745
Size: 32 5720
AlwNI CAG_nnn'CTG
Cuts at: 3034 3495 5067 3034
Size: 461 1572 3719
Fragments arranged by size:
3719 1572 461
ApaLI G'TGCA_C
Cuts at: 3809 4965 3809
Size: 1156 4596
ApoI r'AATT_y
Cuts at: 1493 2178 3061 3287 3439 5548 1493
Size: 685 883 226 152 2109 1697
Fragments arranged by size:
2109 1697 883 685 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4306 160
Size: 872 3274 1606
Fragments arranged by size:
3274 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5752
AvaI C'yCGr_G
Cuts at: 1870 1943 2121 3077 1870
Size: 73 178 956 4545
Fragments arranged by size:
4545 956 178 73
BamHI G'GATC_C
Cuts at: 3082 3449 3082
Size: 367 5385
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3073 4528 618
Size: 325 217 627 1286 1455 1842
Fragments arranged by size:
1842 1455 1286 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2481 2713 3071 1391
Size: 1090 232 358 4072
Fragments arranged by size:
4072 1090 358 232
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4781
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3943 3977 1903
Size: 34 2006 34 3678
Fragments arranged by size:
3678 2006 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3644 4854 3644
Size: 1210 4542
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5752
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2777 4363 136
Size: 107 122 71 631 605 1105 1586 1525
Fragments arranged by size:
1586 1525 1105 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5747 5747
Size: 5752
BlpI GC'TnA_GC
Cuts at: 3169 3169
Size: 5752
Bme1580I G_kGCm'C
Cuts at: 1790 2544 2657 3813 4969 1790
Size: 754 113 1156 1156 2573
Fragments arranged by size:
2573 1156 1156 754 113
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5752
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2990 3483 4441
Size: 416 53 83 399 24 1573 493 958
Cuts at: 4441 442
Size: 1753
Fragments arranged by size:
1753 1573 958 493 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5752
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2627 2733 4412 1239
Size: 1388 106 1679 2579
Fragments arranged by size:
2579 1679 1388 106
Bpu10I CC'TnA_GC
Cuts at: 3027 3027
Size: 5752
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2165 2178 2568 2915 3877 4742 5040 2165
Size: 13 390 347 962 865 298 2877
Fragments arranged by size:
2877 962 865 390 347 298 13
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2118 2264 4415 882
Size: 617 619 146 2151 2219
Fragments arranged by size:
2219 2151 619 617 146
BsaAI yAC'GTr
Cuts at: 493 1204 3543 493
Size: 711 2339 2702
Fragments arranged by size:
2702 2339 711
BsaBI GATnn'nnATC
Cuts at: 2330 5746 2330
Size: 3416 2336
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2012 3559 3941 4576
Size: 53 83 186 936 479 1547 382 635
Cuts at: 4576 275
Size: 1451
Fragments arranged by size:
1547 1451 936 635 479 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4185 4583 4857 5004 5415 4185
Size: 398 274 147 411 4522
Fragments arranged by size:
4522 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2489 2519 1178
Size: 30 165 30 1086 30 4411
Fragments arranged by size:
4411 1086 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5752
BseYI C'CCAG_C
Cuts at: 2672 4955 5451 2672
Size: 2283 496 2973
Fragments arranged by size:
2973 2283 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5179
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3202 3963 4991 1540
Size: 275 186 1201 761 1028 2301
Fragments arranged by size:
2301 1201 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2481 3813 3898 4969 2481
Size: 1332 85 1071 3264
Fragments arranged by size:
3264 1332 1071 85
BsmI GAATG_Cn'
Cuts at: 3263 3356 3263
Size: 93 5659
BsmBI CGTCTCn'nnnn_
Cuts at: 2003 2003
Size: 5752
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2481 2544 2657 2713 3071 3813 3898
Size: 399 691 63 113 56 358 742 85
Cuts at: 3898 4969 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 691 399 358 113 85
63 56
BspEI T'CCGG_A
Cuts at: 5415 5415
Size: 5752
BspHI T'CATG_A
Cuts at: 3641 5371 3641
Size: 1730 4022
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3107 844
Size: 2263 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2845 3639 1394
Size: 429 1022 794 3507
Fragments arranged by size:
3507 1022 794 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4247 4421 65
Size: 1183 2999 174 1396
Fragments arranged by size:
2999 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4396 4583 1700
Size: 110 2586 187 2869
Fragments arranged by size:
2869 2586 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5752
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5752
BssSI C'ACGA_G
Cuts at: 3812 4824 3812
Size: 1012 4740
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5752
BstBI TT'CG_AA
Cuts at: 4556 4556
Size: 5752
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5752
BstXI CCAn_nnnn'nTGG
Cuts at: 2424 2424
Size: 5752
BstYI r'GATC_y
Cuts at: 1972 2164 2950 3082 3449 3834 3851 5303 5389
Size: 192 786 132 367 385 17 1452 86
Cuts at: 5389 5401 5747 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 786 385 367 346 192 132
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2335 2341 2453 513
Size: 552 939 331 6 112 3812
Fragments arranged by size:
3812 939 552 331 112 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5013
Fragments arranged by size:
5013 683 56
BtsI GCAGTG_nn'
Cuts at: 3268 4062 4082 3268
Size: 794 20 4938
Fragments arranged by size:
4938 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5752
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2045 2080 529
Size: 35 1481 35 4201
Fragments arranged by size:
4201 1481 35 35
DraI TTT'AAA
Cuts at: 2862 3056 2862
Size: 194 5558
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4759 809
Size: 507 3443 1802
Fragments arranged by size:
3443 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2338 2631 2854
Size: 54 1425 50 275 18 508 293 223
Cuts at: 2854 3199 4088 5717 8
Size: 345 889 1629 43
Fragments arranged by size:
1629 1425 889 508 345 293 275 223
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3199 1537
Size: 275 1387 4090
Fragments arranged by size:
4090 1387 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2718 3682 1353
Size: 1365 964 3423
Fragments arranged by size:
3423 1365 964
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4339 4713 4859 387
Size: 1064 138 2750 374 146 1280
Fragments arranged by size:
2750 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2479 2479
Size: 5752
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2513 2627 2703 2733 3815 4412 5199
Size: 393 881 114 76 30 1082 597 787
Cuts at: 5199 5467 1239
Size: 268 1524
Fragments arranged by size:
1524 1082 881 787 597 393 268 114
76 30
EcoNI CCTnn'n_nnAGG
Cuts at: 2933 2933
Size: 5752
EcoRI G'AATT_C
Cuts at: 3061 3439 3061
Size: 378 5374
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2543 2543
Size: 5752
FspI TGC'GCA
Cuts at: 3038 4258 3038
Size: 1220 4532
HaeII r_GCGC'y
Cuts at: 1678 4899 1678
Size: 3221 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2541 2573 4393 4425 4467
Size: 32 135 32 969 32 1820 32 42
Cuts at: 4467 4499 1373
Size: 32 2626
Fragments arranged by size:
2626 1820 969 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3096 3342 669
Size: 988 281 1158 246 3079
Fragments arranged by size:
3079 1158 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2280 748
Size: 1532 4220
HpaI GTT'AAC
Cuts at: 3342 3342
Size: 5752
KpnI G_GTAC'C
Cuts at: 3077 3077
Size: 5752
MfeI C'AATT_G
Cuts at: 2028 3351 2028
Size: 1323 4429
MluI A'CGCG_T
Cuts at: 5628 5628
Size: 5752
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2820 4866 5050 1305
Size: 1515 2046 184 2007
Fragments arranged by size:
2046 2007 1515 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2340 2633 2856 10
Size: 54 1425 343 508 293 223 2906
Fragments arranged by size:
2906 1425 508 343 293 223 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3710 4069 4228 518
Size: 648 2544 359 159 2042
Fragments arranged by size:
2544 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2533 3168 3845 4993 5238 1606
Size: 927 635 677 1148 245 2120
Fragments arranged by size:
2120 1148 927 677 635 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2341 2453 513
Size: 552 939 337 112 3812
Fragments arranged by size:
3812 939 552 337 112
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5752
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5752
NotI GC'GGCC_GC
Cuts at: 3199 3199
Size: 5752
NspI r_CATG'y
Cuts at: 1132 1229 3110 4655 1132
Size: 97 1881 1545 2229
Fragments arranged by size:
2229 1881 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5752
PciI A'CATG_T
Cuts at: 4651 4651
Size: 5752
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2347 1108
Size: 1239 4513
PmeI GTTT'AAAC
Cuts at: 3056 3056
Size: 5752
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3821 3853 5334 5366 3821
Size: 32 1481 32 4207
Fragments arranged by size:
4207 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5752
PsiI TTA'TAA
Cuts at: 3322 3322
Size: 5752
PstI C_TGCA'G
Cuts at: 830 3104 830
Size: 2274 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5752
PvuII CAG'CTG
Cuts at: 2533 2533
Size: 5752
SacI G_AGCT'C
Cuts at: 2481 2481
Size: 5752
SalI G'TCGA_C
Cuts at: 1936 3094 1936
Size: 1158 4594
SapI GCTCTTCn'nnn_
Cuts at: 2718 2718
Size: 5752
SbfI CC_TGCA'GG
Cuts at: 3104 3104
Size: 5752
ScaI AGT'ACT
Cuts at: 4000 5712 4000
Size: 1712 4040
SexAI A'CCwGG_T
Cuts at: 2740 2740
Size: 5752
SfcI C'TryA_G
Cuts at: 826 1045 3100 4235 4916 5107 826
Size: 219 2055 1135 681 191 1471
Fragments arranged by size:
2055 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5752
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5752
SmaI CCC'GGG
Cuts at: 1872 3079 1872
Size: 1207 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2144 2193 2583 2930 3856 4757
Size: 197 926 201 49 390 347 926 901
Cuts at: 4757 5019 820
Size: 262 1553
Fragments arranged by size:
1553 926 926 901 390 347 262 201
197 49
SnaBI TAC'GTA
Cuts at: 493 3543 493
Size: 3050 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5752
SphI G_CATG'C
Cuts at: 1132 3110 1132
Size: 1978 3774
SspI AAT'ATT
Cuts at: 5 52 3676 5
Size: 47 3624 2081
Fragments arranged by size:
3624 2081 47
StuI AGG'CCT
Cuts at: 1517 2867 1517
Size: 1350 4402
StyI C'CwwG_G
Cuts at: 513 1065 2004 2341 2453 2899 3191 513
Size: 552 939 337 112 446 292 3074
Fragments arranged by size:
3074 939 552 446 337 292 112
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3779 3796 3949 4134 4579 325
Size: 1255 2199 17 153 185 445 1498
Fragments arranged by size:
2199 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3998 5710 96
Size: 274 80 33 51 1443 2021 1712 138
Fragments arranged by size:
2021 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2489 2521 5712 5744 2489
Size: 32 3191 32 2497
Fragments arranged by size:
3191 2497 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5752
XbaI T'CTAG_A
Cuts at: 2182 3088 2182
Size: 906 4846
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2897 2897
Size: 5752
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5752
XmaI C'CCGG_G
Cuts at: 1870 3077 1870
Size: 1207 4545
XmnI GAAnn'nnTTC
Cuts at: 2664 3881 2664
Size: 1217 4535
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3560 276
Size: 53 83 186 936 2026 2468
Fragments arranged by size:
2468 2026 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoO109I EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacII SanDI SfiI SfoI SrfI SwaI