Restriction map for
pFN21AB8797
(Circular) (Six-base) MAPSORT of: pFN21AB8797.seq Check: 259 from: 1 to: 5704
pFN21AB8797 5704 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2262 2983 2262
Size: 721 4983
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3514 278
Size: 53 83 186 936 1978 2468
Fragments arranged by size:
2468 1978 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3047 1937
Size: 1110 4594
Acc65I G'GTAC_C
Cuts at: 3025 3025
Size: 5704
AclI AA'CG_TT
Cuts at: 1563 3831 4204 1563
Size: 2268 373 3063
Fragments arranged by size:
3063 2268 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3767 5151 5419 1632
Size: 2135 1384 268 1917
Fragments arranged by size:
2135 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5507
AflIII A'CryG_T
Cuts at: 1203 2193 2442 4603 5580 1203
Size: 990 249 2161 977 1327
Fragments arranged by size:
2161 1327 990 977 249
AgeI A'CCGG_T
Cuts at: 4535 4535
Size: 5704
AhdI GACnn_n'nnGTC
Cuts at: 4433 4433
Size: 5704
AleI CACnn'nnGTG
Cuts at: 1166 2576 1166
Size: 1410 4294
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2201 1184
Size: 1017 4687
AlwNI CAG_nnn'CTG
Cuts at: 2068 2561 2998 3447 5019 2068
Size: 493 437 449 1572 2753
Fragments arranged by size:
2753 1572 493 449 437
ApaLI G'TGCA_C
Cuts at: 2249 2397 2722 3761 4917 2249
Size: 148 325 1039 1156 3036
Fragments arranged by size:
3036 1156 1039 325 148
ApoI r'AATT_y
Cuts at: 1493 3013 3239 3391 5500 1493
Size: 1520 226 152 2109 1697
Fragments arranged by size:
2109 1697 1520 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4258 160
Size: 872 3226 1606
Fragments arranged by size:
3226 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5704
AvaI C'yCGr_G
Cuts at: 1870 1943 2266 3029 1870
Size: 73 323 763 4545
Fragments arranged by size:
4545 763 323 73
BamHI G'GATC_C
Cuts at: 3034 3401 3034
Size: 367 5337
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2596 3025 4480 618
Size: 325 217 627 809 429 1455 1842
Fragments arranged by size:
1842 1455 809 627 429 325 217
BanII G_rGCy'C
Cuts at: 1391 2273 2691 2739 3023 1391
Size: 882 418 48 284 4072
Fragments arranged by size:
4072 882 418 284 48
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4733
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3895 3929 1903
Size: 34 1958 34 3678
Fragments arranged by size:
3678 1958 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3596 4806 3596
Size: 1210 4494
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5704
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4315 136
Size: 107 122 71 631 605 2643 1525
Fragments arranged by size:
2643 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5699 5699
Size: 5704
BlpI GC'TnA_GC
Cuts at: 2231 2372 3121 2231
Size: 141 749 4814
Fragments arranged by size:
4814 749 141
Bme1580I G_kGCm'C
Cuts at: 1790 2253 2401 2726 2772 3765 4921 1790
Size: 463 148 325 46 993 1156 2573
Fragments arranged by size:
2573 1156 993 463 325 148 46
BmgBI CAC'GTC
Cuts at: 1318 2194 1318
Size: 876 4828
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3435 4393 442
Size: 416 53 83 399 24 2018 958 1753
Fragments arranged by size:
2018 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5704
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2705 2848 4364 1239
Size: 1466 143 1516 2579
Fragments arranged by size:
2579 1516 1466 143
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3829 4694 4992 3829
Size: 865 298 4541
Fragments arranged by size:
4541 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4367 882
Size: 617 2868 2219
Fragments arranged by size:
2868 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2311 2443 2593 3495 493
Size: 711 1107 132 150 902 2702
Fragments arranged by size:
2702 1107 902 711 150 132
BsaBI GATnn'nnATC
Cuts at: 5698 5698
Size: 5704
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2568 2744 3511 3893
Size: 53 83 186 936 1035 176 767 382
Cuts at: 3893 4528 275
Size: 635 1451
Fragments arranged by size:
1451 1035 936 767 635 382 186 176
83 53
BsaWI w'CCGG_w
Cuts at: 4137 4535 4809 4956 5367 4137
Size: 398 274 147 411 4474
Fragments arranged by size:
4474 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5479
Fragments arranged by size:
5479 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2060 2476 1186
Size: 874 416 4414
Fragments arranged by size:
4414 874 416
BseYI C'CCAG_C
Cuts at: 2074 2098 2764 2915 4907 5403 2074
Size: 24 666 151 1992 496 2375
Fragments arranged by size:
2375 1992 666 496 151 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2810 3008 1163
Size: 573 1074 198 3859
Fragments arranged by size:
3859 1074 573 198
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3154 3915 4943 1540
Size: 275 186 1153 761 1028 2301
Fragments arranged by size:
2301 1153 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2232 2253 2273 2401 2726 3765 3850 4921 2232
Size: 21 20 128 325 1039 85 1071 3015
Fragments arranged by size:
3015 1071 1039 325 128 85 21 20
BsmI GAATG_Cn'
Cuts at: 3215 3308 3215
Size: 93 5611
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2232 2253 2273 2401 2691 2726 2739
Size: 399 442 21 20 128 290 35 13
Cuts at: 2739 2772 3023 3765 3850 4921 1391
Size: 33 251 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 442 399 290 251 128
85 35 33 21 20 13
BspEI T'CCGG_A
Cuts at: 5367 5367
Size: 5704
BspHI T'CATG_A
Cuts at: 3593 5323 3593
Size: 1730 3974
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2076 2262 2983 3059 844
Size: 1232 186 721 76 3489
Fragments arranged by size:
3489 1232 721 186 76
BsrBI CCG'CTC
Cuts at: 1394 1823 3591 1394
Size: 429 1768 3507
Fragments arranged by size:
3507 1768 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4199 4373 65
Size: 1183 2951 174 1396
Fragments arranged by size:
2951 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2679 2958 4348 4535 1700
Size: 110 869 279 1390 187 2869
Fragments arranged by size:
2869 1390 869 279 187 110
BsrGI T'GTAC_A
Cuts at: 96 2427 96
Size: 2331 3373
BssHII G'CGCG_C
Cuts at: 1925 2466 2526 1925
Size: 541 60 5103
Fragments arranged by size:
5103 541 60
BssSI C'ACGA_G
Cuts at: 3764 4776 3764
Size: 1012 4692
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2561 2995 794
Size: 1767 434 3503
Fragments arranged by size:
3503 1767 434
BstBI TT'CG_AA
Cuts at: 4508 4508
Size: 5704
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5704
BstXI CCAn_nnnn'nTGG
Cuts at: 2854 2854
Size: 5704
BstYI r'GATC_y
Cuts at: 1972 2322 3034 3401 3786 3803 5255 5341 5353
Size: 350 712 367 385 17 1452 86 12
Cuts at: 5353 5699 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 712 385 367 350 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2405 2799 513
Size: 552 1340 394 3418
Fragments arranged by size:
3418 1340 552 394
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4965
Fragments arranged by size:
4965 683 56
BtsI GCAGTG_nn'
Cuts at: 2220 2315 2399 2962 3220 4014 4034 2220
Size: 95 84 563 258 794 20 3890
Fragments arranged by size:
3890 794 563 258 95 84 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5704
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5669
DraI TTT'AAA
Cuts at: 3008 3008
Size: 5704
DraIII CAC_nnn'GTG
Cuts at: 2721 2833 2721
Size: 112 5592
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4711 809
Size: 507 3395 1802
Fragments arranged by size:
3395 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2154 2349 2418
Size: 54 1425 50 275 18 324 195 69
Cuts at: 2418 2607 2712 3151 4040 5669 8
Size: 189 105 439 889 1629 43
Fragments arranged by size:
1629 1425 889 439 324 275 195 189
105 69 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3151 1537
Size: 275 1339 4090
Fragments arranged by size:
4090 1339 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2501 3634 1353
Size: 1148 1133 3423
Fragments arranged by size:
3423 1148 1133
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4291 4665 4811 387
Size: 1064 138 2702 374 146 1280
Fragments arranged by size:
2702 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2271 2271
Size: 5704
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2705 2848 3767 4364 5151 5419 1239
Size: 393 1073 143 919 597 787 268 1524
Fragments arranged by size:
1524 1073 919 787 597 393 268 143
EcoO109I rG'GnC_Cy
Cuts at: 2065 2065
Size: 5704
EcoRI G'AATT_C
Cuts at: 3013 3391 3013
Size: 378 5326
FspI TGC'GCA
Cuts at: 4210 4210
Size: 5704
HaeII r_GCGC'y
Cuts at: 1678 2238 2285 4851 1678
Size: 560 47 2566 2531
Fragments arranged by size:
2566 2531 560 47
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4345 4377 4419 4451 1373
Size: 32 135 32 2773 32 42 32 2626
Fragments arranged by size:
2773 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2206 3048 3294 669
Size: 988 281 268 842 246 3079
Fragments arranged by size:
3079 988 842 281 268 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5704
HpaI GTT'AAC
Cuts at: 3294 3294
Size: 5704
KpnI G_GTAC'C
Cuts at: 3029 3029
Size: 5704
MfeI C'AATT_G
Cuts at: 3303 3303
Size: 5704
MluI A'CGCG_T
Cuts at: 5580 5580
Size: 5704
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2950 4818 5002 1305
Size: 1645 1868 184 2007
Fragments arranged by size:
2007 1868 1645 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2156 2351 2420 2609 2714
Size: 54 1425 343 324 195 69 189 105
Cuts at: 2714 10
Size: 3000
Fragments arranged by size:
3000 1425 343 324 195 189 105 69
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2183 2213 2576 2984 3662 4021 4180
Size: 648 1017 30 363 408 678 359 159
Cuts at: 4180 518
Size: 2042
Fragments arranged by size:
2042 1017 678 648 408 363 359 159
30
MspA1I CmG'CkG
Cuts at: 1606 2019 2074 2407 3120 3797 4945 5190 1606
Size: 413 55 333 713 677 1148 245 2120
Fragments arranged by size:
2120 1148 713 677 413 333 245 55
NaeI GCC'GGC
Cuts at: 2681 2681
Size: 5704
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5152
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5704
NgoMIV G'CCGG_C
Cuts at: 2679 2679
Size: 5704
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5704
NotI GC'GGCC_GC
Cuts at: 3151 3151
Size: 5704
NruI TCG'CGA
Cuts at: 2788 2788
Size: 5704
NspI r_CATG'y
Cuts at: 1132 1229 3062 4607 1132
Size: 97 1833 1545 2229
Fragments arranged by size:
2229 1833 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5704
PciI A'CATG_T
Cuts at: 4603 4603
Size: 5704
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5704
PmeI GTTT'AAAC
Cuts at: 3008 3008
Size: 5704
PmlI CAC'GTG
Cuts at: 2311 2443 2311
Size: 132 5572
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3773 3805 5286 5318 3773
Size: 32 1481 32 4159
Fragments arranged by size:
4159 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2065 2065
Size: 5704
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5704
PsiI TTA'TAA
Cuts at: 3274 3274
Size: 5704
PstI C_TGCA'G
Cuts at: 830 2018 2073 2322 2510 3056 830
Size: 1188 55 249 188 546 3478
Fragments arranged by size:
3478 1188 546 249 188 55
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5704
PvuII CAG'CTG
Cuts at: 2019 2074 2019
Size: 55 5649
SacI G_AGCT'C
Cuts at: 2273 2273
Size: 5704
SacII CC_GC'GG
Cuts at: 2408 2408
Size: 5704
SalI G'TCGA_C
Cuts at: 1936 3046 1936
Size: 1110 4594
SapI GCTCTTCn'nnn_
Cuts at: 2501 2501
Size: 5704
SbfI CC_TGCA'GG
Cuts at: 3056 3056
Size: 5704
ScaI AGT'ACT
Cuts at: 3952 5664 3952
Size: 1712 3992
SfcI C'TryA_G
Cuts at: 826 1045 2014 2069 2318 2506 3052 4187 4868
Size: 219 969 55 249 188 546 1135 681
Cuts at: 4868 5059 826
Size: 191 1471
Fragments arranged by size:
1471 1135 969 681 546 249 219 191
188 55
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5704
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5704
SmaI CCC'GGG
Cuts at: 1872 3031 1872
Size: 1159 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3808 4709 4971 820
Size: 197 926 1865 901 262 1553
Fragments arranged by size:
1865 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3495 493
Size: 3002 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5704
SphI G_CATG'C
Cuts at: 1132 3062 1132
Size: 1930 3774
SspI AAT'ATT
Cuts at: 5 52 3628 5
Size: 47 3576 2081
Fragments arranged by size:
3576 2081 47
StuI AGG'CCT
Cuts at: 1517 2223 1517
Size: 706 4998
StyI C'CwwG_G
Cuts at: 513 1065 3143 513
Size: 552 2078 3074
Fragments arranged by size:
3074 2078 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3731 3748 3901 4086 4531 325
Size: 1255 2151 17 153 185 445 1498
Fragments arranged by size:
2151 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2196 2355 2427
Size: 274 80 33 51 1443 219 159 72
Cuts at: 2427 3950 5662 96
Size: 1523 1712 138
Fragments arranged by size:
1712 1523 1443 274 219 159 138 80
72 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2307 2339 5664 5696 2307
Size: 32 3325 32 2315
Fragments arranged by size:
3325 2315 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5704
XbaI T'CTAG_A
Cuts at: 3040 3040
Size: 5704
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5704
XmaI C'CCGG_G
Cuts at: 1870 3029 1870
Size: 1159 4545
XmnI GAAnn'nnTTC
Cuts at: 2840 3833 2840
Size: 993 4711
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3512 276
Size: 53 83 186 936 1978 2468
Fragments arranged by size:
2468 1978 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NruI NspI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI
EcoRV FalI FseI FspAI KasI NarI NsiI PacI
PfoI PspOMI PspXI PsrI RsrII SanDI SexAI SfiI
SfoI SrfI SwaI XcmI