Restriction map for
pFN21AB8796
(Circular) (Six-base) MAPSORT of: pFN21AB8796.seq Check: 5341 from: 1 to: 5704
pFN21AB8796 5704 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3514 278
Size: 53 83 186 936 1978 2468
Fragments arranged by size:
2468 1978 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2565 3047 1937
Size: 628 482 4594
Fragments arranged by size:
4594 628 482
Acc65I G'GTAC_C
Cuts at: 2550 3025 2550
Size: 475 5229
AclI AA'CG_TT
Cuts at: 1563 3831 4204 1563
Size: 2268 373 3063
Fragments arranged by size:
3063 2268 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2039 3767 5151 5419 1632
Size: 407 1728 1384 268 1917
Fragments arranged by size:
1917 1728 1384 407 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5507
AflIII A'CryG_T
Cuts at: 1203 2902 4603 5580 1203
Size: 1699 1701 977 1327
Fragments arranged by size:
1701 1699 1327 977
AgeI A'CCGG_T
Cuts at: 4535 4535
Size: 5704
AhdI GACnn_n'nnGTC
Cuts at: 4433 4433
Size: 5704
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5704
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5704
AlwNI CAG_nnn'CTG
Cuts at: 2752 3447 5019 2752
Size: 695 1572 3437
Fragments arranged by size:
3437 1572 695
ApaLI G'TGCA_C
Cuts at: 3761 4917 3761
Size: 1156 4548
ApoI r'AATT_y
Cuts at: 1493 3013 3239 3391 5500 1493
Size: 1520 226 152 2109 1697
Fragments arranged by size:
2109 1697 1520 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4258 160
Size: 872 3226 1606
Fragments arranged by size:
3226 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5704
AvaI C'yCGr_G
Cuts at: 1870 1943 3029 1870
Size: 73 1086 4545
Fragments arranged by size:
4545 1086 73
BamHI G'GATC_C
Cuts at: 3034 3401 3034
Size: 367 5337
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2550 2661 2938 2972 2990
Size: 325 217 627 763 111 277 34 18
Cuts at: 2990 3025 4480 618
Size: 35 1455 1842
Fragments arranged by size:
1842 1455 763 627 325 277 217 111
35 34 18
BanII G_rGCy'C
Cuts at: 1391 2405 3023 1391
Size: 1014 618 4072
Fragments arranged by size:
4072 1014 618
BbeI G_GCGC'C
Cuts at: 2665 2994 2665
Size: 329 5375
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2860 928
Size: 971 961 3772
Fragments arranged by size:
3772 971 961
BbvCI CC'TCA_GC
Cuts at: 2135 2711 2135
Size: 576 5128
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3895 3929 1903
Size: 34 1958 34 3678
Fragments arranged by size:
3678 1958 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3596 4806 3596
Size: 1210 4494
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5704
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2145 4315 136
Size: 107 122 71 631 605 473 2170 1525
Fragments arranged by size:
2170 1525 631 605 473 122 107 71
BglII A'GATC_T
Cuts at: 5699 5699
Size: 5704
BlpI GC'TnA_GC
Cuts at: 3121 3121
Size: 5704
Bme1580I G_kGCm'C
Cuts at: 1790 3765 4921 1790
Size: 1975 1156 2573
Fragments arranged by size:
2573 1975 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5704
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2290 3435 4393
Size: 416 53 83 399 24 873 1145 958
Cuts at: 4393 442
Size: 1753
Fragments arranged by size:
1753 1145 958 873 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5704
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2321 3010 2321
Size: 689 5015
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2210 4364 1239
Size: 971 2154 2579
Fragments arranged by size:
2579 2154 971
Bpu10I CC'TnA_GC
Cuts at: 2135 2711 2135
Size: 576 5128
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3829 4694 4992 3829
Size: 865 298 4541
Fragments arranged by size:
4541 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4367 882
Size: 617 2868 2219
Fragments arranged by size:
2868 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2547 2620 3495 493
Size: 711 1343 73 875 2702
Fragments arranged by size:
2702 1343 875 711 73
BsaBI GATnn'nnATC
Cuts at: 5698 5698
Size: 5704
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2662 2991 3511 3893
Size: 53 83 186 936 1129 329 520 382
Cuts at: 3893 4528 275
Size: 635 1451
Fragments arranged by size:
1451 1129 936 635 520 382 329 186
83 53
BsaWI w'CCGG_w
Cuts at: 2772 4137 4535 4809 4956 5367 2772
Size: 1365 398 274 147 411 3109
Fragments arranged by size:
3109 1365 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2543 2573 2892 2922 1178
Size: 30 165 30 1140 30 319 30 3960
Fragments arranged by size:
3960 1140 319 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2123 2306 1186
Size: 937 183 4584
Fragments arranged by size:
4584 937 183
BseYI C'CCAG_C
Cuts at: 2286 2293 2715 2942 2947 4907 5403 2286
Size: 7 422 227 5 1960 496 2587
Fragments arranged by size:
2587 1960 496 422 227 7 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5131
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3154 3915 4943 1540
Size: 275 186 1153 761 1028 2301
Fragments arranged by size:
2301 1153 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2033 2862 3765 3850 4921 2033
Size: 829 903 85 1071 2816
Fragments arranged by size:
2816 1071 903 829 85
BsmI GAATG_Cn'
Cuts at: 3215 3308 3215
Size: 93 5611
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2033 2405 2862 3023 3765 3850 4921
Size: 399 243 372 457 161 742 85 1071
Cuts at: 4921 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 457 399 372 243 161
85
BspEI T'CCGG_A
Cuts at: 2772 5367 2772
Size: 2595 3109
BspHI T'CATG_A
Cuts at: 2370 3593 5323 2370
Size: 1223 1730 2751
Fragments arranged by size:
2751 1730 1223
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3059 844
Size: 2215 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2646 3591 1394
Size: 429 823 945 3507
Fragments arranged by size:
3507 945 823 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4199 4373 65
Size: 1183 2951 174 1396
Fragments arranged by size:
2951 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2928 4348 4535 1700
Size: 110 1118 1420 187 2869
Fragments arranged by size:
2869 1420 1118 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5704
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5704
BssSI C'ACGA_G
Cuts at: 3764 4776 3764
Size: 1012 4692
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5704
BstBI TT'CG_AA
Cuts at: 4508 4508
Size: 5704
BstEII G'GTnAC_C
Cuts at: 1184 2449 1184
Size: 1265 4439
BstXI CCAn_nnnn'nTGG
Cuts at: 2280 2698 2969 2280
Size: 418 271 5015
Fragments arranged by size:
5015 418 271
BstYI r'GATC_y
Cuts at: 1972 2265 2625 3034 3401 3786 3803 5255 5341
Size: 293 360 409 367 385 17 1452 86
Cuts at: 5341 5353 5699 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 409 385 367 360 346 293
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2014 2630 2014
Size: 616 5088
BtgI C'CryG_G
Cuts at: 513 1065 2004 2454 513
Size: 552 939 450 3763
Fragments arranged by size:
3763 939 552 450
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4965
Fragments arranged by size:
4965 683 56
BtsI GCAGTG_nn'
Cuts at: 2839 3220 4014 4034 2839
Size: 381 794 20 4509
Fragments arranged by size:
4509 794 381 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5704
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5669
DraI TTT'AAA
Cuts at: 3008 3008
Size: 5704
DraIII CAC_nnn'GTG
Cuts at: 2512 2512
Size: 5704
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4711 809
Size: 507 3395 1802
Fragments arranged by size:
3395 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3151 4040 5669
Size: 54 1425 50 275 18 1321 889 1629
Cuts at: 5669 8
Size: 43
Fragments arranged by size:
1629 1425 1321 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3151 1537
Size: 275 1339 4090
Fragments arranged by size:
4090 1339 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2585 3634 1353
Size: 1232 1049 3423
Fragments arranged by size:
3423 1232 1049
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4291 4665 4811 387
Size: 1064 138 2702 374 146 1280
Fragments arranged by size:
2702 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2039 2210 3767 4364 5151 5419 1239
Size: 393 407 171 1557 597 787 268 1524
Fragments arranged by size:
1557 1524 787 597 407 393 268 171
EcoO109I rG'GnC_Cy
Cuts at: 2167 2634 2720 2167
Size: 467 86 5151
Fragments arranged by size:
5151 467 86
EcoRI G'AATT_C
Cuts at: 3013 3391 3013
Size: 378 5326
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2701 2701
Size: 5704
FspI TGC'GCA
Cuts at: 4210 4210
Size: 5704
HaeII r_GCGC'y
Cuts at: 1678 2665 2994 4851 1678
Size: 987 329 1857 2531
Fragments arranged by size:
2531 1857 987 329
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4345 4377 4419 4451 1373
Size: 32 135 32 2773 32 42 32 2626
Fragments arranged by size:
2773 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3048 3294 669
Size: 988 281 1110 246 3079
Fragments arranged by size:
3079 1110 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5704
HpaI GTT'AAC
Cuts at: 3294 3294
Size: 5704
KasI G'GCGC_C
Cuts at: 2661 2990 2661
Size: 329 5375
KpnI G_GTAC'C
Cuts at: 2554 3029 2554
Size: 475 5229
MfeI C'AATT_G
Cuts at: 3303 3303
Size: 5704
MluI A'CGCG_T
Cuts at: 5580 5580
Size: 5704
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4818 5002 1305
Size: 3513 184 2007
Fragments arranged by size:
3513 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3882
Fragments arranged by size:
3882 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2459 2696 3662 4021 4180 518
Size: 648 1293 237 966 359 159 2042
Fragments arranged by size:
2042 1293 966 648 359 237 159
MspA1I CmG'CkG
Cuts at: 1606 2436 2715 3120 3797 4945 5190 1606
Size: 830 279 405 677 1148 245 2120
Fragments arranged by size:
2120 1148 830 677 405 279 245
NaeI GCC'GGC
Cuts at: 2930 2930
Size: 5704
NarI GG'CG_CC
Cuts at: 2662 2991 2662
Size: 329 5375
NcoI C'CATG_G
Cuts at: 513 1065 2004 2454 513
Size: 552 939 450 3763
Fragments arranged by size:
3763 939 552 450
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5704
NgoMIV G'CCGG_C
Cuts at: 2928 2928
Size: 5704
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5704
NotI GC'GGCC_GC
Cuts at: 3151 3151
Size: 5704
NspI r_CATG'y
Cuts at: 1132 1229 2906 3062 4607 1132
Size: 97 1677 156 1545 2229
Fragments arranged by size:
2229 1677 1545 156 97
PasI CC'CwG_GG
Cuts at: 1348 2596 1348
Size: 1248 4456
PciI A'CATG_T
Cuts at: 2902 4603 2902
Size: 1701 4003
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5704
PmeI GTTT'AAAC
Cuts at: 3008 3008
Size: 5704
PmlI CAC'GTG
Cuts at: 2547 2547
Size: 5704
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3773 3805 5286 5318 3773
Size: 32 1481 32 4159
Fragments arranged by size:
4159 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2167 2634 2720 2167
Size: 467 86 5151
Fragments arranged by size:
5151 467 86
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5704
PsiI TTA'TAA
Cuts at: 3274 3274
Size: 5704
PstI C_TGCA'G
Cuts at: 830 2748 2937 3056 830
Size: 1918 189 119 3478
Fragments arranged by size:
3478 1918 189 119
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5704
PvuII CAG'CTG
Cuts at: 2715 2715
Size: 5704
SalI G'TCGA_C
Cuts at: 1936 3046 1936
Size: 1110 4594
SanDI GG'GwC_CC
Cuts at: 2720 2720
Size: 5704
SbfI CC_TGCA'GG
Cuts at: 3056 3056
Size: 5704
ScaI AGT'ACT
Cuts at: 3952 5664 3952
Size: 1712 3992
SexAI A'CCwGG_T
Cuts at: 2235 2235
Size: 5704
SfcI C'TryA_G
Cuts at: 826 1045 2344 2744 2933 3052 4187 4868 5059
Size: 219 1299 400 189 119 1135 681 191
Cuts at: 5059 826
Size: 1471
Fragments arranged by size:
1471 1299 1135 681 400 219 191 189
119
SfoI GGC'GCC
Cuts at: 2663 2992 2663
Size: 329 5375
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5704
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5704
SmaI CCC'GGG
Cuts at: 1872 3031 1872
Size: 1159 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3808 4709 4971 820
Size: 197 926 1865 901 262 1553
Fragments arranged by size:
1865 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3495 493
Size: 3002 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5704
SphI G_CATG'C
Cuts at: 1132 3062 1132
Size: 1930 3774
SspI AAT'ATT
Cuts at: 5 52 3628 5
Size: 47 3576 2081
Fragments arranged by size:
3576 2081 47
StuI AGG'CCT
Cuts at: 1517 2081 1517
Size: 564 5140
StyI C'CwwG_G
Cuts at: 513 1065 2004 2454 3143 513
Size: 552 939 450 689 3074
Fragments arranged by size:
3074 939 689 552 450
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2278 2956 3731 3748 3901 4086 4531
Size: 1255 698 678 775 17 153 185 445
Cuts at: 4531 325
Size: 1498
Fragments arranged by size:
1498 1255 775 698 678 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3950 5662 96
Size: 274 80 33 51 1443 1973 1712 138
Fragments arranged by size:
1973 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5664 5696 5664
Size: 32 5672
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5704
XbaI T'CTAG_A
Cuts at: 3040 3040
Size: 5704
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5704
XmaI C'CCGG_G
Cuts at: 1870 3029 1870
Size: 1159 4545
XmnI GAAnn'nnTTC
Cuts at: 3833 3833
Size: 5704
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3512 276
Size: 53 83 186 936 1978 2468
Fragments arranged by size:
2468 1978 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV FseI FspAI
NruI NsiI PacI PfoI PspOMI PspXI PsrI RsrII
SacI SacII SapI SfiI SrfI SwaI XcmI