Restriction map for
pFN21AB8795
(Circular) (Six-base) MAPSORT of: pFN21AB8795.seq Check: 9018 from: 1 to: 5698
pFN21AB8795 5698 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3508 278
Size: 53 83 186 936 1972 2468
Fragments arranged by size:
2468 1972 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3041 1937
Size: 1104 4594
Acc65I G'GTAC_C
Cuts at: 3019 3019
Size: 5698
AclI AA'CG_TT
Cuts at: 1563 3825 4198 1563
Size: 2262 373 3063
Fragments arranged by size:
3063 2262 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2333 2836 3761 5145 5413 1632
Size: 701 503 925 1384 268 1917
Fragments arranged by size:
1917 1384 925 701 503 268
AflII C'TTAA_G
Cuts at: 820 1017 2689 820
Size: 197 1672 3829
Fragments arranged by size:
3829 1672 197
AflIII A'CryG_T
Cuts at: 1203 2597 4597 5574 1203
Size: 1394 2000 977 1327
Fragments arranged by size:
2000 1394 1327 977
AgeI A'CCGG_T
Cuts at: 4529 4529
Size: 5698
AhdI GACnn_n'nnGTC
Cuts at: 4427 4427
Size: 5698
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5698
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5698
AlwNI CAG_nnn'CTG
Cuts at: 3441 5013 3441
Size: 1572 4126
ApaLI G'TGCA_C
Cuts at: 3755 4911 3755
Size: 1156 4542
ApoI r'AATT_y
Cuts at: 1493 2023 2634 3007 3233 3385 5494 1493
Size: 530 611 373 226 152 2109 1697
Fragments arranged by size:
2109 1697 611 530 373 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4252 160
Size: 872 3220 1606
Fragments arranged by size:
3220 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5698
AvaI C'yCGr_G
Cuts at: 1870 1943 3023 1870
Size: 73 1080 4545
Fragments arranged by size:
4545 1080 73
BamHI G'GATC_C
Cuts at: 3028 3395 3028
Size: 367 5331
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2373 3019 4474 618
Size: 325 217 627 586 646 1455 1842
Fragments arranged by size:
1842 1455 646 627 586 325 217
BanII G_rGCy'C
Cuts at: 1391 3017 1391
Size: 1626 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4727
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3889 3923 1903
Size: 34 1952 34 3678
Fragments arranged by size:
3678 1952 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3590 4800 3590
Size: 1210 4488
BclI T'GATC_A
Cuts at: 1548 2791 1548
Size: 1243 4455
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4309 136
Size: 107 122 71 631 605 2637 1525
Fragments arranged by size:
2637 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5693 5693
Size: 5698
BlpI GC'TnA_GC
Cuts at: 3115 3115
Size: 5698
Bme1580I G_kGCm'C
Cuts at: 1790 3759 4915 1790
Size: 1969 1156 2573
Fragments arranged by size:
2573 1969 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5698
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3429 4387 442
Size: 416 53 83 399 24 2012 958 1753
Fragments arranged by size:
2012 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5698
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2664 2832 4358 1239
Size: 1425 168 1526 2579
Fragments arranged by size:
2579 1526 1425 168
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2850 2902 3823 4688 4986 2850
Size: 52 921 865 298 3562
Fragments arranged by size:
3562 921 865 298 52
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4361 882
Size: 617 2862 2219
Fragments arranged by size:
2862 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3489 493
Size: 711 2285 2702
Fragments arranged by size:
2702 2285 711
BsaBI GATnn'nnATC
Cuts at: 5692 5692
Size: 5698
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3505 3887 4522 275
Size: 53 83 186 936 1972 382 635 1451
Fragments arranged by size:
1972 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2474 4131 4529 4803 4950 5361 2474
Size: 1657 398 274 147 411 2811
Fragments arranged by size:
2811 1657 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5473
Fragments arranged by size:
5473 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2023 2996 1186
Size: 837 973 3888
Fragments arranged by size:
3888 973 837
BseYI C'CCAG_C
Cuts at: 4901 5397 4901
Size: 496 5202
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5125
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3148 3909 4937 1540
Size: 275 186 1147 761 1028 2301
Fragments arranged by size:
2301 1147 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3759 3844 4915 3759
Size: 85 1071 4542
Fragments arranged by size:
4542 1071 85
BsmI GAATG_Cn'
Cuts at: 2556 3209 3302 2556
Size: 653 93 4952
Fragments arranged by size:
4952 653 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3017 3759 3844 4915 1391
Size: 399 1227 742 85 1071 2174
Fragments arranged by size:
2174 1227 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5361 5361
Size: 5698
BspHI T'CATG_A
Cuts at: 3587 5317 3587
Size: 1730 3968
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3053 844
Size: 2209 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3585 1394
Size: 429 1762 3507
Fragments arranged by size:
3507 1762 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4193 4367 65
Size: 1183 2945 174 1396
Fragments arranged by size:
2945 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4342 4529 1700
Size: 110 2532 187 2869
Fragments arranged by size:
2869 2532 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5698
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5698
BssSI C'ACGA_G
Cuts at: 3758 4770 3758
Size: 1012 4686
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5698
BstBI TT'CG_AA
Cuts at: 4502 4502
Size: 5698
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5698
BstYI r'GATC_y
Cuts at: 1972 3028 3395 3780 3797 5249 5335 5347 5693
Size: 1056 367 385 17 1452 86 12 346
Cuts at: 5693 1972
Size: 1977
Fragments arranged by size:
1977 1452 1056 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5146
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4959
Fragments arranged by size:
4959 683 56
BtsI GCAGTG_nn'
Cuts at: 2335 3214 4008 4028 2335
Size: 879 794 20 4005
Fragments arranged by size:
4005 879 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5698
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5663
DraI TTT'AAA
Cuts at: 3002 3002
Size: 5698
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4705 809
Size: 507 3389 1802
Fragments arranged by size:
3389 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3145 4034 5663
Size: 54 1425 50 275 18 1315 889 1629
Cuts at: 5663 8
Size: 43
Fragments arranged by size:
1629 1425 1315 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3145 1537
Size: 275 1333 4090
Fragments arranged by size:
4090 1333 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3628 1353
Size: 2275 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4285 4659 4805 387
Size: 1064 138 2696 374 146 1280
Fragments arranged by size:
2696 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2333 2664 2832 2836 3761 4358 5145
Size: 393 701 331 168 4 925 597 787
Cuts at: 5145 5413 1239
Size: 268 1524
Fragments arranged by size:
1524 925 787 701 597 393 331 268
168 4
EcoRI G'AATT_C
Cuts at: 2634 3007 3385 2634
Size: 373 378 4947
Fragments arranged by size:
4947 378 373
FspI TGC'GCA
Cuts at: 4204 4204
Size: 5698
HaeII r_GCGC'y
Cuts at: 1678 4845 1678
Size: 3167 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2066 2098 2707 2739 4339
Size: 32 135 32 494 32 609 32 1600
Cuts at: 4339 4371 4413 4445 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1600 609 494 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3042 3288 669
Size: 988 281 1104 246 3079
Fragments arranged by size:
3079 1104 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2259 748
Size: 1511 4187
HpaI GTT'AAC
Cuts at: 3288 3288
Size: 5698
KpnI G_GTAC'C
Cuts at: 3023 3023
Size: 5698
MfeI C'AATT_G
Cuts at: 3297 3297
Size: 5698
MluI A'CGCG_T
Cuts at: 5574 5574
Size: 5698
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2417 4812 4996 1305
Size: 1112 2395 184 2007
Fragments arranged by size:
2395 2007 1112 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3876
Fragments arranged by size:
3876 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3656 4015 4174 518
Size: 648 2490 359 159 2042
Fragments arranged by size:
2490 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3114 3791 4939 5184 1606
Size: 1508 677 1148 245 2120
Fragments arranged by size:
2120 1508 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5146
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5698
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5698
NotI GC'GGCC_GC
Cuts at: 3145 3145
Size: 5698
NspI r_CATG'y
Cuts at: 1132 1229 2328 2601 3056 4601 1132
Size: 97 1099 273 455 1545 2229
Fragments arranged by size:
2229 1545 1099 455 273 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5698
PciI A'CATG_T
Cuts at: 2597 4597 2597
Size: 2000 3698
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5698
PmeI GTTT'AAAC
Cuts at: 3002 3002
Size: 5698
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3767 3799 5280 5312 3767
Size: 32 1481 32 4153
Fragments arranged by size:
4153 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2097 1613
Size: 484 5214
PsiI TTA'TAA
Cuts at: 3268 3268
Size: 5698
PstI C_TGCA'G
Cuts at: 830 3050 830
Size: 2220 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5698
SalI G'TCGA_C
Cuts at: 1936 3040 1936
Size: 1104 4594
SbfI CC_TGCA'GG
Cuts at: 3050 3050
Size: 5698
ScaI AGT'ACT
Cuts at: 2058 3946 5658 2058
Size: 1888 1712 2098
Fragments arranged by size:
2098 1888 1712
SfcI C'TryA_G
Cuts at: 826 1045 3046 4181 4862 5053 826
Size: 219 2001 1135 681 191 1471
Fragments arranged by size:
2001 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5698
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5698
SmaI CCC'GGG
Cuts at: 1872 3025 1872
Size: 1153 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2689 2865 2881 3802 4703 4965
Size: 197 926 746 176 16 921 901 262
Cuts at: 4965 820
Size: 1553
Fragments arranged by size:
1553 926 921 901 746 262 197 176
16
SnaBI TAC'GTA
Cuts at: 493 3489 493
Size: 2996 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5698
SphI G_CATG'C
Cuts at: 1132 3056 1132
Size: 1924 3774
SspI AAT'ATT
Cuts at: 5 52 3622 5
Size: 47 3570 2081
Fragments arranged by size:
3570 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5698
StyI C'CwwG_G
Cuts at: 513 1065 3137 513
Size: 552 2072 3074
Fragments arranged by size:
3074 2072 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3725 3742 3895 4080 4525 325
Size: 1255 2145 17 153 185 445 1498
Fragments arranged by size:
2145 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2056 2159 2203
Size: 274 80 33 51 1443 79 103 44
Cuts at: 2203 3944 5656 96
Size: 1741 1712 138
Fragments arranged by size:
1741 1712 1443 274 138 103 80 79
51 44 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2584 2616 5658 5690 2584
Size: 32 3042 32 2592
Fragments arranged by size:
3042 2592 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5698
XbaI T'CTAG_A
Cuts at: 3034 3034
Size: 5698
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5698
XmaI C'CCGG_G
Cuts at: 1870 3023 1870
Size: 1153 4545
XmnI GAAnn'nnTTC
Cuts at: 2345 2849 3827 2345
Size: 504 978 4216
Fragments arranged by size:
4216 978 504
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3506 276
Size: 53 83 186 936 1972 2468
Fragments arranged by size:
2468 1972 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI