Restriction map for
pFN21AB8792
(Circular) (Six-base) MAPSORT of: pFN21AB8792.seq Check: 4429 from: 1 to: 5686
pFN21AB8792 5686 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3496 278
Size: 53 83 186 936 1960 2468
Fragments arranged by size:
2468 1960 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3029 1937
Size: 1092 4594
Acc65I G'GTAC_C
Cuts at: 3007 3007
Size: 5686
AclI AA'CG_TT
Cuts at: 1563 3813 4186 1563
Size: 2250 373 3063
Fragments arranged by size:
3063 2250 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3749 5133 5401 1632
Size: 2117 1384 268 1917
Fragments arranged by size:
2117 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5489
AflIII A'CryG_T
Cuts at: 1203 4585 5562 1203
Size: 3382 977 1327
Fragments arranged by size:
3382 1327 977
AgeI A'CCGG_T
Cuts at: 4517 4517
Size: 5686
AhdI GACnn_n'nnGTC
Cuts at: 4415 4415
Size: 5686
AleI CACnn'nnGTG
Cuts at: 1166 2172 2256 2507 2759 2843 1166
Size: 1006 84 251 252 84 4009
Fragments arranged by size:
4009 1006 252 251 84 84
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2031 1184
Size: 847 4839
AlwNI CAG_nnn'CTG
Cuts at: 2045 3429 5001 2045
Size: 1384 1572 2730
Fragments arranged by size:
2730 1572 1384
ApaLI G'TGCA_C
Cuts at: 2307 2642 3743 4899 2307
Size: 335 1101 1156 3094
Fragments arranged by size:
3094 1156 1101 335
ApoI r'AATT_y
Cuts at: 1493 2163 2331 2414 2498 2666 2750 2834 2995
Size: 670 168 83 84 168 84 84 161
Cuts at: 2995 3221 3373 5482 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 670 226 168 168 161 152
84 84 84 83
AseI AT'TA_AT
Cuts at: 160 1032 4240 160
Size: 872 3208 1606
Fragments arranged by size:
3208 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5686
AvaI C'yCGr_G
Cuts at: 1870 1943 3011 1870
Size: 73 1068 4545
Fragments arranged by size:
4545 1068 73
BamHI G'GATC_C
Cuts at: 3016 3383 3016
Size: 367 5319
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3007 4462 618
Size: 325 217 627 1220 1455 1842
Fragments arranged by size:
1842 1455 1220 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3005 1391
Size: 1614 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4715
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3877 3911 1903
Size: 34 50 34 1856 34 3678
Fragments arranged by size:
3678 1856 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3578 4788 3578
Size: 1210 4476
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5686
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4297 136
Size: 107 122 71 631 605 2625 1525
Fragments arranged by size:
2625 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5681 5681
Size: 5686
BlpI GC'TnA_GC
Cuts at: 3103 3103
Size: 5686
Bme1580I G_kGCm'C
Cuts at: 1790 2143 2311 2646 3747 4903 1790
Size: 353 168 335 1101 1156 2573
Fragments arranged by size:
2573 1156 1101 353 335 168
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5686
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3417 4375 442
Size: 416 53 83 399 24 2000 958 1753
Fragments arranged by size:
2000 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5686
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4346 1239
Size: 3107 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3811 4676 4974 3811
Size: 865 298 4523
Fragments arranged by size:
4523 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4349 882
Size: 617 2850 2219
Fragments arranged by size:
2850 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3477 493
Size: 711 2273 2702
Fragments arranged by size:
2702 2273 711
BsaBI GATnn'nnATC
Cuts at: 5680 5680
Size: 5686
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3493 3875 4510 275
Size: 53 83 186 936 1960 382 635 1451
Fragments arranged by size:
1960 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4119 4517 4791 4938 5349 4119
Size: 398 274 147 411 4456
Fragments arranged by size:
4456 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5461
Fragments arranged by size:
5461 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5686
BseYI C'CCAG_C
Cuts at: 4889 5385 4889
Size: 496 5190
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5113
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3136 3897 4925 1540
Size: 275 186 1135 761 1028 2301
Fragments arranged by size:
2301 1135 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2311 2646 3747 3832 4903 2311
Size: 335 1101 85 1071 3094
Fragments arranged by size:
3094 1101 1071 335 85
BsmI GAATG_Cn'
Cuts at: 2195 2545 3197 3290 2195
Size: 350 652 93 4591
Fragments arranged by size:
4591 652 350 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2143 2311 2646 3005 3747 3832 4903
Size: 399 353 168 335 359 742 85 1071
Cuts at: 4903 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 359 353 335 168
85
BspEI T'CCGG_A
Cuts at: 5349 5349
Size: 5686
BspHI T'CATG_A
Cuts at: 3575 5305 3575
Size: 1730 3956
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3041 844
Size: 2197 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3573 1394
Size: 429 1750 3507
Fragments arranged by size:
3507 1750 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2025 2372 4181 4355 65
Size: 1183 777 347 1809 174 1396
Fragments arranged by size:
1809 1396 1183 777 347 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4330 4517 1700
Size: 110 2520 187 2869
Fragments arranged by size:
2869 2520 187 110
BsrGI T'GTAC_A
Cuts at: 96 2443 96
Size: 2347 3339
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5686
BssSI C'ACGA_G
Cuts at: 3746 4758 3746
Size: 1012 4674
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2045 794
Size: 1251 4435
BstBI TT'CG_AA
Cuts at: 4490 4490
Size: 5686
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5686
BstYI r'GATC_y
Cuts at: 1972 3016 3383 3768 3785 5237 5323 5335 5681
Size: 1044 367 385 17 1452 86 12 346
Cuts at: 5681 1972
Size: 1977
Fragments arranged by size:
1977 1452 1044 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5134
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4947
Fragments arranged by size:
4947 683 56
BtsI GCAGTG_nn'
Cuts at: 2875 3202 3996 4016 2875
Size: 327 794 20 4545
Fragments arranged by size:
4545 794 327 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5686
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2182 2217 529
Size: 35 1618 35 3998
Fragments arranged by size:
3998 1618 35 35
DraI TTT'AAA
Cuts at: 2028 2990 2028
Size: 962 4724
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4693 809
Size: 507 3377 1802
Fragments arranged by size:
3377 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3133 4022 5651
Size: 54 1425 50 275 18 1303 889 1629
Cuts at: 5651 8
Size: 43
Fragments arranged by size:
1629 1425 1303 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3133 1537
Size: 275 1321 4090
Fragments arranged by size:
4090 1321 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3616 1353
Size: 2263 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2317 4273 4647 4793 387
Size: 1064 138 728 1956 374 146 1280
Fragments arranged by size:
1956 1280 1064 728 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3749 4346 5133 5401 1239
Size: 393 2117 597 787 268 1524
Fragments arranged by size:
2117 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2163 2331 2498 2666 2750 2834 2995 3373 2163
Size: 168 167 168 84 84 161 378 4476
Fragments arranged by size:
4476 378 168 168 167 161 84 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2982 2982
Size: 5686
FspI TGC'GCA
Cuts at: 4192 4192
Size: 5686
HaeII r_GCGC'y
Cuts at: 1678 4833 1678
Size: 3155 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2296 2328 4327 4359 4401
Size: 32 135 32 724 32 1999 32 42
Cuts at: 4401 4433 1373
Size: 32 2626
Fragments arranged by size:
2626 1999 724 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3030 3276 669
Size: 988 281 1092 246 3079
Fragments arranged by size:
3079 1092 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5686
HpaI GTT'AAC
Cuts at: 3276 3276
Size: 5686
KpnI G_GTAC'C
Cuts at: 3011 3011
Size: 5686
MfeI C'AATT_G
Cuts at: 2805 3285 2805
Size: 480 5206
MluI A'CGCG_T
Cuts at: 5562 5562
Size: 5686
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2351 2920 4800 4984 1305
Size: 1046 569 1880 184 2007
Fragments arranged by size:
2007 1880 1046 569 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3864
Fragments arranged by size:
3864 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2172 2188 2256 2340 2423 2507 2591
Size: 648 1006 16 68 84 83 84 84
Cuts at: 2591 2675 2691 2759 2843 2927 3644 4003 4162
Size: 84 16 68 84 84 717 359 159
Cuts at: 4162 518
Size: 2042
Fragments arranged by size:
2042 1006 717 648 359 159 84 84
84 84 84 84 83 68 68 16
16
MspA1I CmG'CkG
Cuts at: 1606 3102 3779 4927 5172 1606
Size: 1496 677 1148 245 2120
Fragments arranged by size:
2120 1496 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5134
NdeI CA'TA_TG
Cuts at: 387 2185 2688 387
Size: 1798 503 3385
Fragments arranged by size:
3385 1798 503
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5686
NotI GC'GGCC_GC
Cuts at: 3133 3133
Size: 5686
NspI r_CATG'y
Cuts at: 1132 1229 3044 4589 1132
Size: 97 1815 1545 2229
Fragments arranged by size:
2229 1815 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5686
PciI A'CATG_T
Cuts at: 4585 4585
Size: 5686
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5686
PmeI GTTT'AAAC
Cuts at: 2990 2990
Size: 5686
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3755 3787 5268 5300 3755
Size: 32 1481 32 4141
Fragments arranged by size:
4141 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5686
PsiI TTA'TAA
Cuts at: 2355 3256 2355
Size: 901 4785
PstI C_TGCA'G
Cuts at: 830 3038 830
Size: 2208 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5686
SalI G'TCGA_C
Cuts at: 1936 3028 1936
Size: 1092 4594
SbfI CC_TGCA'GG
Cuts at: 3038 3038
Size: 5686
ScaI AGT'ACT
Cuts at: 3934 5646 3934
Size: 1712 3974
SfcI C'TryA_G
Cuts at: 826 1045 3034 4169 4850 5041 826
Size: 219 1989 1135 681 191 1471
Fragments arranged by size:
1989 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5686
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5686
SmaI CCC'GGG
Cuts at: 1872 3013 1872
Size: 1141 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3790 4691 4953 820
Size: 197 926 1847 901 262 1553
Fragments arranged by size:
1847 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3477 493
Size: 2984 2702
SpeI A'CTAG_T
Cuts at: 152 2255 152
Size: 2103 3583
SphI G_CATG'C
Cuts at: 1132 3044 1132
Size: 1912 3774
SspI AAT'ATT
Cuts at: 5 52 3610 5
Size: 47 3558 2081
Fragments arranged by size:
3558 2081 47
StuI AGG'CCT
Cuts at: 1517 2378 2965 1517
Size: 861 587 4238
Fragments arranged by size:
4238 861 587
StyI C'CwwG_G
Cuts at: 513 1065 3125 513
Size: 552 2060 3074
Fragments arranged by size:
3074 2060 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3713 3730 3883 4068 4513 325
Size: 1255 2133 17 153 185 445 1498
Fragments arranged by size:
2133 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2443 3932 5644
Size: 274 80 33 51 1443 466 1489 1712
Cuts at: 5644 96
Size: 138
Fragments arranged by size:
1712 1489 1443 466 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5646 5678 5646
Size: 32 5654
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5686
XbaI T'CTAG_A
Cuts at: 3022 3022
Size: 5686
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5686
XmaI C'CCGG_G
Cuts at: 1870 3011 1870
Size: 1141 4545
XmnI GAAnn'nnTTC
Cuts at: 2713 2965 3815 2713
Size: 252 850 4584
Fragments arranged by size:
4584 850 252
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3494 276
Size: 53 83 186 936 1960 2468
Fragments arranged by size:
2468 1960 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI