Restriction map for
pFN21AB8791
(Circular) (Six-base) MAPSORT of: pFN21AB8791.seq Check: 4142 from: 1 to: 5662
pFN21AB8791 5662 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3472 278
Size: 53 83 186 936 1936 2468
Fragments arranged by size:
2468 1936 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3005 1937
Size: 1068 4594
Acc65I G'GTAC_C
Cuts at: 2983 2983
Size: 5662
AclI AA'CG_TT
Cuts at: 1563 3789 4162 1563
Size: 2226 373 3063
Fragments arranged by size:
3063 2226 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2122 3725 5109 5377 1632
Size: 490 1603 1384 268 1917
Fragments arranged by size:
1917 1603 1384 490 268
AflII C'TTAA_G
Cuts at: 820 1017 2150 820
Size: 197 1133 4332
Fragments arranged by size:
4332 1133 197
AflIII A'CryG_T
Cuts at: 1203 2502 2517 4561 5538 1203
Size: 1299 15 2044 977 1327
Fragments arranged by size:
2044 1327 1299 977 15
AgeI A'CCGG_T
Cuts at: 2358 4493 2358
Size: 2135 3527
AhdI GACnn_n'nnGTC
Cuts at: 4391 4391
Size: 5662
AleI CACnn'nnGTG
Cuts at: 1166 2291 1166
Size: 1125 4537
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5662
AlwNI CAG_nnn'CTG
Cuts at: 2755 3405 4977 2755
Size: 650 1572 3440
Fragments arranged by size:
3440 1572 650
ApaI G_GGCC'C
Cuts at: 2908 2908
Size: 5662
ApaLI G'TGCA_C
Cuts at: 2284 3719 4875 2284
Size: 1435 1156 3071
Fragments arranged by size:
3071 1435 1156
ApoI r'AATT_y
Cuts at: 1493 2885 2971 3197 3349 5458 1493
Size: 1392 86 226 152 2109 1697
Fragments arranged by size:
2109 1697 1392 226 152 86
AseI AT'TA_AT
Cuts at: 160 1032 4216 160
Size: 872 3184 1606
Fragments arranged by size:
3184 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5662
AvaI C'yCGr_G
Cuts at: 1870 1943 2955 2987 1870
Size: 73 1012 32 4545
Fragments arranged by size:
4545 1012 73 32
BamHI G'GATC_C
Cuts at: 2992 3359 2992
Size: 367 5295
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2983 4438 618
Size: 325 217 627 1196 1455 1842
Fragments arranged by size:
1842 1455 1196 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2908 2981 1391
Size: 1517 73 4072
Fragments arranged by size:
4072 1517 73
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2323 2539 2698 928
Size: 971 424 216 159 3892
Fragments arranged by size:
3892 971 424 216 159
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2156 2190 3853 3887 1903
Size: 34 219 34 1663 34 3678
Fragments arranged by size:
3678 1663 219 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3554 4764 3554
Size: 1210 4452
BclI T'GATC_A
Cuts at: 1548 2193 1548
Size: 645 5017
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2699 4273 136
Size: 107 122 71 631 605 1027 1574 1525
Fragments arranged by size:
1574 1525 1027 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2415 5657 2415
Size: 3242 2420
BlpI GC'TnA_GC
Cuts at: 3079 3079
Size: 5662
Bme1580I G_kGCm'C
Cuts at: 1790 2288 2908 3723 4879 1790
Size: 498 620 815 1156 2573
Fragments arranged by size:
2573 1156 815 620 498
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5662
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2665 2720 3393
Size: 416 53 83 399 24 1248 55 673
Cuts at: 3393 4351 442
Size: 958 1753
Fragments arranged by size:
1753 1248 958 673 416 399 83 55
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5662
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2554 2579 2593 4322 1239
Size: 1315 25 14 1729 2579
Fragments arranged by size:
2579 1729 1315 25 14
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3787 4652 4950 3787
Size: 865 298 4499
Fragments arranged by size:
4499 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2634 2719 4325 882
Size: 617 1135 85 1606 2219
Fragments arranged by size:
2219 1606 1135 617 85
BsaAI yAC'GTr
Cuts at: 493 1204 3453 493
Size: 711 2249 2702
Fragments arranged by size:
2702 2249 711
BsaBI GATnn'nnATC
Cuts at: 5656 5656
Size: 5662
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3469 3851 4486 275
Size: 53 83 186 936 1936 382 635 1451
Fragments arranged by size:
1936 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2358 2400 2456 4095 4493 4767 4914 5325 2358
Size: 42 56 1639 398 274 147 411 2695
Fragments arranged by size:
2695 1639 411 398 274 147 56 42
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5437
Fragments arranged by size:
5437 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2102 1186
Size: 916 4746
BseYI C'CCAG_C
Cuts at: 2629 4865 5361 2629
Size: 2236 496 2930
Fragments arranged by size:
2930 2236 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2416 2681 2803 1163
Size: 573 680 265 122 4022
Fragments arranged by size:
4022 680 573 265 122
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3112 3873 4901 1540
Size: 275 186 1111 761 1028 2301
Fragments arranged by size:
2301 1111 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2229 2288 3723 3808 4879 2229
Size: 59 1435 85 1071 3012
Fragments arranged by size:
3012 1435 1071 85 59
BsmI GAATG_Cn'
Cuts at: 3173 3266 3173
Size: 93 5569
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2229 2288 2908 2981 3723 3808 4879
Size: 399 439 59 620 73 742 85 1071
Cuts at: 4879 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 620 439 399 85 73
59
BspEI T'CCGG_A
Cuts at: 5325 5325
Size: 5662
BspHI T'CATG_A
Cuts at: 3551 5281 3551
Size: 1730 3932
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2127 2223 3017 844
Size: 1283 96 794 3489
Fragments arranged by size:
3489 1283 794 96
BsrBI CCG'CTC
Cuts at: 1394 1823 2383 3549 1394
Size: 429 560 1166 3507
Fragments arranged by size:
3507 1166 560 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4157 4331 65
Size: 1183 2909 174 1396
Fragments arranged by size:
2909 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2358 2910 4306 4493 1700
Size: 110 548 552 1396 187 2869
Fragments arranged by size:
2869 1396 552 548 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5662
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5662
BssSI C'ACGA_G
Cuts at: 3722 4734 3722
Size: 1012 4650
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5662
BstBI TT'CG_AA
Cuts at: 4466 4466
Size: 5662
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5662
BstYI r'GATC_y
Cuts at: 1972 2415 2424 2992 3359 3744 3761 5213 5299
Size: 443 9 568 367 385 17 1452 86
Cuts at: 5299 5311 5657 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 568 443 385 367 346 86
17 12 9
BtgI C'CryG_G
Cuts at: 513 1065 2131 2482 2583 513
Size: 552 1066 351 101 3592
Fragments arranged by size:
3592 1066 552 351 101
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2671 487
Size: 683 56 1445 3478
Fragments arranged by size:
3478 1445 683 56
BtsI GCAGTG_nn'
Cuts at: 3178 3972 3992 3178
Size: 794 20 4848
Fragments arranged by size:
4848 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5662
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5627
DraI TTT'AAA
Cuts at: 2966 2966
Size: 5662
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4669 809
Size: 507 3353 1802
Fragments arranged by size:
3353 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2124 2367 3109
Size: 54 1425 50 275 18 294 243 742
Cuts at: 3109 3998 5627 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 742 294 275 243 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3109 1537
Size: 275 1297 4090
Fragments arranged by size:
4090 1297 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3592 1353
Size: 2239 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4249 4623 4769 387
Size: 1064 138 2660 374 146 1280
Fragments arranged by size:
2660 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2122 2554 2579 2593 3725 4322 5109
Size: 393 490 432 25 14 1132 597 787
Cuts at: 5109 5377 1239
Size: 268 1524
Fragments arranged by size:
1524 1132 787 597 490 432 393 268
25 14
EcoNI CCTnn'n_nnAGG
Cuts at: 2255 2255
Size: 5662
EcoO109I rG'GnC_Cy
Cuts at: 2443 2904 2443
Size: 461 5201
EcoRI G'AATT_C
Cuts at: 2885 2971 3349 2885
Size: 86 378 5198
Fragments arranged by size:
5198 378 86
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2566 2566
Size: 5662
FspI TGC'GCA
Cuts at: 4168 4168
Size: 5662
HaeII r_GCGC'y
Cuts at: 1678 2541 4809 1678
Size: 863 2268 2531
Fragments arranged by size:
2531 2268 863
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4303 4335 4377 4409 1373
Size: 32 135 32 2731 32 42 32 2626
Fragments arranged by size:
2731 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2050 2842 3006 3252 669
Size: 988 281 112 792 164 246 3079
Fragments arranged by size:
3079 988 792 281 246 164 112
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5662
HpaI GTT'AAC
Cuts at: 3252 3252
Size: 5662
KpnI G_GTAC'C
Cuts at: 2987 2987
Size: 5662
MfeI C'AATT_G
Cuts at: 2816 3261 2816
Size: 445 5217
MluI A'CGCG_T
Cuts at: 5538 5538
Size: 5662
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4776 4960 1305
Size: 3471 184 2007
Fragments arranged by size:
3471 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2126 2369 10
Size: 54 1425 343 294 243 3303
Fragments arranged by size:
3303 1425 343 294 243 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2189 2291 2351 3620 3979 4138 518
Size: 648 1023 102 60 1269 359 159 2042
Fragments arranged by size:
2042 1269 1023 648 359 159 102 60
MspA1I CmG'CkG
Cuts at: 1606 2557 2929 3078 3755 4903 5148 1606
Size: 951 372 149 677 1148 245 2120
Fragments arranged by size:
2120 1148 951 677 372 245 149
NcoI C'CATG_G
Cuts at: 513 1065 2131 2482 2583 513
Size: 552 1066 351 101 3592
Fragments arranged by size:
3592 1066 552 351 101
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5662
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5662
NotI GC'GGCC_GC
Cuts at: 3109 3109
Size: 5662
NspI r_CATG'y
Cuts at: 1132 1229 2320 2506 2521 3020 4565 1132
Size: 97 1091 186 15 499 1545 2229
Fragments arranged by size:
2229 1545 1091 499 186 97 15
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5662
PciI A'CATG_T
Cuts at: 2502 2517 4561 2502
Size: 15 2044 3603
Fragments arranged by size:
3603 2044 15
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5662
PfoI T'CCnGG_A
Cuts at: 2609 2609
Size: 5662
PmeI GTTT'AAAC
Cuts at: 2966 2966
Size: 5662
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3731 3763 5244 5276 3731
Size: 32 1481 32 4117
Fragments arranged by size:
4117 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2443 2443
Size: 5662
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5662
PsiI TTA'TAA
Cuts at: 3232 3232
Size: 5662
PspOMI G'GGCC_C
Cuts at: 2904 2904
Size: 5662
PstI C_TGCA'G
Cuts at: 830 2751 2940 3014 830
Size: 1921 189 74 3478
Fragments arranged by size:
3478 1921 189 74
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5662
PvuII CAG'CTG
Cuts at: 2929 2929
Size: 5662
SalI G'TCGA_C
Cuts at: 1936 3004 1936
Size: 1068 4594
SbfI CC_TGCA'GG
Cuts at: 2940 3014 2940
Size: 74 5588
ScaI AGT'ACT
Cuts at: 3910 5622 3910
Size: 1712 3950
SfcI C'TryA_G
Cuts at: 826 1045 2069 2473 2680 2747 2936 3010 4145
Size: 219 1024 404 207 67 189 74 1135
Cuts at: 4145 4826 5017 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1024 681 404 219 207 191
189 74 67
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5662
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5662
SmaI CCC'GGG
Cuts at: 1872 2957 2989 1872
Size: 1085 32 4545
Fragments arranged by size:
4545 1085 32
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2150 3766 4667 4929 820
Size: 197 926 207 1616 901 262 1553
Fragments arranged by size:
1616 1553 926 901 262 207 197
SnaBI TAC'GTA
Cuts at: 493 3453 493
Size: 2960 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5662
SphI G_CATG'C
Cuts at: 1132 3020 1132
Size: 1888 3774
SspI AAT'ATT
Cuts at: 5 52 3586 5
Size: 47 3534 2081
Fragments arranged by size:
3534 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5662
StyI C'CwwG_G
Cuts at: 513 1065 2131 2482 2541 2583 3101 513
Size: 552 1066 351 59 42 518 3074
Fragments arranged by size:
3074 1066 552 518 351 59 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2647 2663 3689 3706 3859 4044 4489
Size: 1255 1067 16 1026 17 153 185 445
Cuts at: 4489 325
Size: 1498
Fragments arranged by size:
1498 1255 1067 1026 445 185 153 17
16
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3908 5620 96
Size: 274 80 33 51 1443 1931 1712 138
Fragments arranged by size:
1931 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2387 2419 2900 2932 5622 5654 2387
Size: 32 481 32 2690 32 2395
Fragments arranged by size:
2690 2395 481 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2045 1316
Size: 729 4933
XbaI T'CTAG_A
Cuts at: 2998 2998
Size: 5662
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2098 2443 2590 2926 2098
Size: 345 147 336 4834
Fragments arranged by size:
4834 345 336 147
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5662
XmaI C'CCGG_G
Cuts at: 1870 2955 2987 1870
Size: 1085 32 4545
Fragments arranged by size:
4545 1085 32
XmnI GAAnn'nnTTC
Cuts at: 3791 3791
Size: 5662
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3470 276
Size: 53 83 186 936 1936 2468
Fragments arranged by size:
2468 1936 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI