Restriction map for
pFN21AB8785
(Circular) (Six-base) MAPSORT of: pFN21AB8785.seq Check: 8887 from: 1 to: 5584
pFN21AB8785 5584 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2279 2279
Size: 5584
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3394 278
Size: 53 83 186 936 1858 2468
Fragments arranged by size:
2468 1858 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2927 1937
Size: 990 4594
Acc65I G'GTAC_C
Cuts at: 2905 2905
Size: 5584
AclI AA'CG_TT
Cuts at: 1563 2016 3711 4084 1563
Size: 453 1695 373 3063
Fragments arranged by size:
3063 1695 453 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2528 3647 5031 5299 1632
Size: 896 1119 1384 268 1917
Fragments arranged by size:
1917 1384 1119 896 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5387
AflIII A'CryG_T
Cuts at: 1203 2389 4483 5460 1203
Size: 1186 2094 977 1327
Fragments arranged by size:
2094 1327 1186 977
AgeI A'CCGG_T
Cuts at: 4415 4415
Size: 5584
AhdI GACnn_n'nnGTC
Cuts at: 4313 4313
Size: 5584
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5584
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5584
AlwNI CAG_nnn'CTG
Cuts at: 3327 4899 3327
Size: 1572 4012
ApaLI G'TGCA_C
Cuts at: 3641 4797 3641
Size: 1156 4428
ApoI r'AATT_y
Cuts at: 1493 2893 3119 3271 5380 1493
Size: 1400 226 152 2109 1697
Fragments arranged by size:
2109 1697 1400 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4138 160
Size: 872 3106 1606
Fragments arranged by size:
3106 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5584
AvaI C'yCGr_G
Cuts at: 1870 1943 2193 2909 1870
Size: 73 250 716 4545
Fragments arranged by size:
4545 716 250 73
BamHI G'GATC_C
Cuts at: 2032 2914 3281 2032
Size: 882 367 4335
Fragments arranged by size:
4335 882 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2905 4360 618
Size: 325 217 627 1118 1455 1842
Fragments arranged by size:
1842 1455 1118 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2903 1391
Size: 1512 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2745 928
Size: 971 846 3767
Fragments arranged by size:
3767 971 846
BbvCI CC'TCA_GC
Cuts at: 2748 2835 2748
Size: 87 5497
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3775 3809 1903
Size: 34 1838 34 3678
Fragments arranged by size:
3678 1838 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3476 4686 3476
Size: 1210 4374
BclI T'GATC_A
Cuts at: 1548 2811 1548
Size: 1263 4321
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4195 136
Size: 107 122 71 631 605 2523 1525
Fragments arranged by size:
2523 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5579 5579
Size: 5584
BlpI GC'TnA_GC
Cuts at: 3001 3001
Size: 5584
Bme1580I G_kGCm'C
Cuts at: 1790 2430 3645 4801 1790
Size: 640 1215 1156 2573
Fragments arranged by size:
2573 1215 1156 640
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5584
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3315 4273 442
Size: 416 53 83 399 24 1898 958 1753
Fragments arranged by size:
1898 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2235 1056
Size: 1179 4405
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2825 4244 1239
Size: 1586 1419 2579
Fragments arranged by size:
2579 1586 1419
Bpu10I CC'TnA_GC
Cuts at: 2748 2835 2748
Size: 87 5497
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3709 4574 4872 3709
Size: 865 298 4421
Fragments arranged by size:
4421 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4247 882
Size: 617 2748 2219
Fragments arranged by size:
2748 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2390 3375 493
Size: 711 1186 985 2702
Fragments arranged by size:
2702 1186 985 711
BsaBI GATnn'nnATC
Cuts at: 5578 5578
Size: 5584
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3391 3773 4408 275
Size: 53 83 186 936 1858 382 635 1451
Fragments arranged by size:
1858 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4017 4415 4689 4836 5247 4017
Size: 398 274 147 411 4354
Fragments arranged by size:
4354 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5359
Fragments arranged by size:
5359 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2314 2388 2391 2394 2684 2765 1186
Size: 1128 74 3 3 290 81 4005
Fragments arranged by size:
4005 1128 290 81 74 3 3
BseYI C'CCAG_C
Cuts at: 2383 4787 5283 2383
Size: 2404 496 2684
Fragments arranged by size:
2684 2404 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2754 1163
Size: 573 1018 3993
Fragments arranged by size:
3993 1018 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3034 3795 4823 1540
Size: 275 186 1033 761 1028 2301
Fragments arranged by size:
2301 1033 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3645 3730 4801 3645
Size: 85 1071 4428
Fragments arranged by size:
4428 1071 85
BsmI GAATG_Cn'
Cuts at: 3095 3188 3095
Size: 93 5491
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2430 2903 3645 3730 4801 1391
Size: 399 640 473 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 640 473 399 85
BspEI T'CCGG_A
Cuts at: 5247 5247
Size: 5584
BspHI T'CATG_A
Cuts at: 3473 5203 3473
Size: 1730 3854
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2279 2939 844
Size: 1435 660 3489
Fragments arranged by size:
3489 1435 660
BsrBI CCG'CTC
Cuts at: 1394 1823 3471 1394
Size: 429 1648 3507
Fragments arranged by size:
3507 1648 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2409 4079 4253 65
Size: 1183 1161 1670 174 1396
Fragments arranged by size:
1670 1396 1183 1161 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4228 4415 1700
Size: 110 2418 187 2869
Fragments arranged by size:
2869 2418 187 110
BsrGI T'GTAC_A
Cuts at: 96 2373 96
Size: 2277 3307
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5584
BssSI C'ACGA_G
Cuts at: 3644 4656 3644
Size: 1012 4572
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5584
BstBI TT'CG_AA
Cuts at: 4388 4388
Size: 5584
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5584
BstXI CCAn_nnnn'nTGG
Cuts at: 2029 2416 2029
Size: 387 5197
BstYI r'GATC_y
Cuts at: 1972 2032 2507 2914 3281 3666 3683 5135 5221
Size: 60 475 407 367 385 17 1452 86
Cuts at: 5221 5233 5579 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 475 407 385 367 346 86
60 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4093
Fragments arranged by size:
4093 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4845
Fragments arranged by size:
4845 683 56
BtsI GCAGTG_nn'
Cuts at: 3100 3894 3914 3100
Size: 794 20 4770
Fragments arranged by size:
4770 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5584
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5549
DraI TTT'AAA
Cuts at: 2888 2888
Size: 5584
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4591 809
Size: 507 3275 1802
Fragments arranged by size:
3275 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2869 3031
Size: 54 1425 50 275 18 177 862 162
Cuts at: 3031 3920 5549 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 862 275 177 162 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3031 1537
Size: 275 1219 4090
Fragments arranged by size:
4090 1219 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2661 3514 1353
Size: 1308 853 3423
Fragments arranged by size:
3423 1308 853
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4171 4545 4691 387
Size: 1064 138 2582 374 146 1280
Fragments arranged by size:
2582 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2528 2825 3647 4244 5031 5299 1239
Size: 393 896 297 822 597 787 268 1524
Fragments arranged by size:
1524 896 822 787 597 393 297 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2464 2464
Size: 5584
EcoO109I rG'GnC_Cy
Cuts at: 2758 2758
Size: 5584
EcoRI G'AATT_C
Cuts at: 2893 3271 2893
Size: 378 5206
FspI TGC'GCA
Cuts at: 4090 4090
Size: 5584
HaeII r_GCGC'y
Cuts at: 1678 4731 1678
Size: 3053 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2020 2024 2052 2056 4225
Size: 32 135 32 448 4 28 4 2169
Cuts at: 4225 4257 4299 4331 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2169 448 135 42 32 32 32
32 28 4 4
HincII GTy'rAC
Cuts at: 669 1657 1938 2441 2565 2928 3174 669
Size: 988 281 503 124 363 246 3079
Fragments arranged by size:
3079 988 503 363 281 246 124
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5584
HpaI GTT'AAC
Cuts at: 3174 3174
Size: 5584
KpnI G_GTAC'C
Cuts at: 2909 2909
Size: 5584
MfeI C'AATT_G
Cuts at: 3183 3183
Size: 5584
MluI A'CGCG_T
Cuts at: 5460 5460
Size: 5584
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4698 4882 1305
Size: 3393 184 2007
Fragments arranged by size:
3393 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2871 10
Size: 54 1425 343 177 862 2723
Fragments arranged by size:
2723 1425 862 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3542 3901 4060 518
Size: 648 2376 359 159 2042
Fragments arranged by size:
2376 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2309 2803 3000 3677 4825 5070 1606
Size: 703 494 197 677 1148 245 2120
Fragments arranged by size:
2120 1148 703 677 494 245 197
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4093
Fragments arranged by size:
4093 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5584
NheI G'CTAG_C
Cuts at: 1052 2231 1052
Size: 1179 4405
NotI GC'GGCC_GC
Cuts at: 3031 3031
Size: 5584
NspI r_CATG'y
Cuts at: 1132 1229 2942 4487 1132
Size: 97 1713 1545 2229
Fragments arranged by size:
2229 1713 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5584
PciI A'CATG_T
Cuts at: 4483 4483
Size: 5584
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5584
PfoI T'CCnGG_A
Cuts at: 2595 2595
Size: 5584
PmeI GTTT'AAAC
Cuts at: 2888 2888
Size: 5584
PmlI CAC'GTG
Cuts at: 2390 2390
Size: 5584
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3653 3685 5166 5198 3653
Size: 32 1481 32 4039
Fragments arranged by size:
4039 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2758 2758
Size: 5584
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5584
PsiI TTA'TAA
Cuts at: 3154 3154
Size: 5584
PstI C_TGCA'G
Cuts at: 830 2936 830
Size: 2106 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5584
PvuII CAG'CTG
Cuts at: 2803 2803
Size: 5584
SalI G'TCGA_C
Cuts at: 1936 2926 1936
Size: 990 4594
SanDI GG'GwC_CC
Cuts at: 2758 2758
Size: 5584
SbfI CC_TGCA'GG
Cuts at: 2936 2936
Size: 5584
ScaI AGT'ACT
Cuts at: 3832 5544 3832
Size: 1712 3872
SfcI C'TryA_G
Cuts at: 826 1045 2202 2421 2932 4067 4748 4939 826
Size: 219 1157 219 511 1135 681 191 1471
Fragments arranged by size:
1471 1157 1135 681 511 219 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5584
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5584
SmaI CCC'GGG
Cuts at: 1872 2195 2911 1872
Size: 323 716 4545
Fragments arranged by size:
4545 716 323
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3688 4589 4851 820
Size: 197 926 1745 901 262 1553
Fragments arranged by size:
1745 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3375 493
Size: 2882 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5584
SphI G_CATG'C
Cuts at: 1132 2942 1132
Size: 1810 3774
SrfI GCCC'GGGC
Cuts at: 2195 2195
Size: 5584
SspI AAT'ATT
Cuts at: 5 52 3508 5
Size: 47 3456 2081
Fragments arranged by size:
3456 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5584
StyI C'CwwG_G
Cuts at: 513 1065 2004 3023 513
Size: 552 939 1019 3074
Fragments arranged by size:
3074 1019 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3611 3628 3781 3966 4411 325
Size: 1255 2031 17 153 185 445 1498
Fragments arranged by size:
2031 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2373 3830 5542
Size: 274 80 33 51 1443 396 1457 1712
Cuts at: 5542 96
Size: 138
Fragments arranged by size:
1712 1457 1443 396 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5544 5576 5544
Size: 32 5552
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5584
XbaI T'CTAG_A
Cuts at: 2920 2920
Size: 5584
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5584
XmaI C'CCGG_G
Cuts at: 1870 2193 2909 1870
Size: 323 716 4545
Fragments arranged by size:
4545 716 323
XmnI GAAnn'nnTTC
Cuts at: 3713 3713
Size: 5584
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3392 276
Size: 53 83 186 936 1858 2468
Fragments arranged by size:
2468 1858 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PspOMI PspXI PsrI RsrII SacI SacII
SapI SexAI SfiI SfoI SwaI XcmI