Restriction map for
pFN21AB8783
(Circular) (Six-base) MAPSORT of: pFN21AB8783.seq Check: 3326 from: 1 to: 5536
pFN21AB8783 5536 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2398 2398
Size: 5536
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3346 278
Size: 53 83 186 936 1810 2468
Fragments arranged by size:
2468 1810 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2283 2879 1937
Size: 346 596 4594
Fragments arranged by size:
4594 596 346
Acc65I G'GTAC_C
Cuts at: 2857 2857
Size: 5536
AclI AA'CG_TT
Cuts at: 1563 3663 4036 1563
Size: 2100 373 3063
Fragments arranged by size:
3063 2100 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2219 3599 4983 5251 1632
Size: 587 1380 1384 268 1917
Fragments arranged by size:
1917 1384 1380 587 268
AflII C'TTAA_G
Cuts at: 820 1017 2656 820
Size: 197 1639 3700
Fragments arranged by size:
3700 1639 197
AflIII A'CryG_T
Cuts at: 1203 4435 5412 1203
Size: 3232 977 1327
Fragments arranged by size:
3232 1327 977
AgeI A'CCGG_T
Cuts at: 4367 4367
Size: 5536
AhdI GACnn_n'nnGTC
Cuts at: 4265 4265
Size: 5536
AleI CACnn'nnGTG
Cuts at: 1166 2603 2618 1166
Size: 1437 15 4084
Fragments arranged by size:
4084 1437 15
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2130 2636 1184
Size: 946 506 4084
Fragments arranged by size:
4084 946 506
AlwNI CAG_nnn'CTG
Cuts at: 2763 3279 4851 2763
Size: 516 1572 3448
Fragments arranged by size:
3448 1572 516
ApaLI G'TGCA_C
Cuts at: 2262 3593 4749 2262
Size: 1331 1156 3049
Fragments arranged by size:
3049 1331 1156
ApoI r'AATT_y
Cuts at: 1493 2829 2845 3071 3223 5332 1493
Size: 1336 16 226 152 2109 1697
Fragments arranged by size:
2109 1697 1336 226 152 16
AseI AT'TA_AT
Cuts at: 160 1032 4090 160
Size: 872 3058 1606
Fragments arranged by size:
3058 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5536
AvaI C'yCGr_G
Cuts at: 1870 1943 2014 2141 2552 2861 1870
Size: 73 71 127 411 309 4545
Fragments arranged by size:
4545 411 309 127 73 71
BamHI G'GATC_C
Cuts at: 2866 3233 2866
Size: 367 5169
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2410 2857 4312 618
Size: 325 217 627 623 447 1455 1842
Fragments arranged by size:
1842 1455 627 623 447 325 217
BanII G_rGCy'C
Cuts at: 1391 2798 2855 1391
Size: 1407 57 4072
Fragments arranged by size:
4072 1407 57
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4565
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3727 3761 1903
Size: 34 1790 34 3678
Fragments arranged by size:
3678 1790 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2203 3428 4638 2203
Size: 1225 1210 3101
Fragments arranged by size:
3101 1225 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5536
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4147 136
Size: 107 122 71 631 605 2475 1525
Fragments arranged by size:
2475 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5531 5531
Size: 5536
BlpI GC'TnA_GC
Cuts at: 2953 2953
Size: 5536
Bme1580I G_kGCm'C
Cuts at: 1790 2266 2415 3597 4753 1790
Size: 476 149 1182 1156 2573
Fragments arranged by size:
2573 1182 1156 476 149
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5536
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2643 3267 4225
Size: 416 53 83 399 24 1226 624 958
Cuts at: 4225 442
Size: 1753
Fragments arranged by size:
1753 1226 958 624 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5536
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2117 4196 1239
Size: 878 2079 2579
Fragments arranged by size:
2579 2079 878
Bpu10I CC'TnA_GC
Cuts at: 2688 2688
Size: 5536
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2033 2654 3661 4526 4824 2033
Size: 621 1007 865 298 2745
Fragments arranged by size:
2745 1007 865 621 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4199 882
Size: 617 2700 2219
Fragments arranged by size:
2700 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3327 493
Size: 711 2123 2702
Fragments arranged by size:
2702 2123 711
BsaBI GATnn'nnATC
Cuts at: 5530 5530
Size: 5536
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3343 3725 4360 275
Size: 53 83 186 936 1810 382 635 1451
Fragments arranged by size:
1810 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3969 4367 4641 4788 5199 3969
Size: 398 274 147 411 4306
Fragments arranged by size:
4306 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5311
Fragments arranged by size:
5311 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5536
BseYI C'CCAG_C
Cuts at: 4739 5235 4739
Size: 496 5040
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2136 2596 2794 1163
Size: 573 400 460 198 3905
Fragments arranged by size:
3905 573 460 400 198
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2986 3747 4775 1540
Size: 275 186 985 761 1028 2301
Fragments arranged by size:
2301 1028 985 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2266 3597 3682 4753 2266
Size: 1331 85 1071 3049
Fragments arranged by size:
3049 1331 1071 85
BsmI GAATG_Cn'
Cuts at: 2396 2704 3047 3140 2396
Size: 308 343 93 4792
Fragments arranged by size:
4792 343 308 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2266 2415 2798 2855 3597 3682 4753
Size: 399 476 149 383 57 742 85 1071
Cuts at: 4753 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 476 399 383 149 85
57
BspEI T'CCGG_A
Cuts at: 5199 5199
Size: 5536
BspHI T'CATG_A
Cuts at: 3425 5155 3425
Size: 1730 3806
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2398 2891 844
Size: 1554 493 3489
Fragments arranged by size:
3489 1554 493
BsrBI CCG'CTC
Cuts at: 1394 1823 2247 2644 3423 1394
Size: 429 424 397 779 3507
Fragments arranged by size:
3507 779 429 424 397
BsrDI GCAATG_nn'
Cuts at: 65 1248 2289 4031 4205 65
Size: 1183 1041 1742 174 1396
Fragments arranged by size:
1742 1396 1183 1041 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4180 4367 1700
Size: 110 2370 187 2869
Fragments arranged by size:
2869 2370 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5536
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5536
BssSI C'ACGA_G
Cuts at: 2256 3596 4608 2256
Size: 1340 1012 3184
Fragments arranged by size:
3184 1340 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2763 794
Size: 1969 3567
BstBI TT'CG_AA
Cuts at: 4340 4340
Size: 5536
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5536
BstXI CCAn_nnnn'nTGG
Cuts at: 2176 2176
Size: 5536
BstYI r'GATC_y
Cuts at: 1972 2091 2866 3233 3618 3635 5087 5173 5185
Size: 119 775 367 385 17 1452 86 12
Cuts at: 5185 5531 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 775 385 367 346 119 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2682 513
Size: 552 1617 3367
Fragments arranged by size:
3367 1617 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4797
Fragments arranged by size:
4797 683 56
BtsI GCAGTG_nn'
Cuts at: 3052 3846 3866 3052
Size: 794 20 4722
Fragments arranged by size:
4722 794 20
ClaI AT'CG_AT
Cuts at: 1554 2323 1554
Size: 769 4767
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5501
DraI TTT'AAA
Cuts at: 2840 2840
Size: 5536
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4543 809
Size: 507 3227 1802
Fragments arranged by size:
3227 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2182 2514 2983
Size: 54 1425 50 275 18 352 332 469
Cuts at: 2983 3872 5501 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 469 352 332 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2983 1537
Size: 275 1171 4090
Fragments arranged by size:
4090 1171 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3466 1353
Size: 2113 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4123 4497 4643 387
Size: 1064 138 2534 374 146 1280
Fragments arranged by size:
2534 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2117 2219 3599 4196 4983 5251 1239
Size: 393 485 102 1380 597 787 268 1524
Fragments arranged by size:
1524 1380 787 597 485 393 268 102
EcoO109I rG'GnC_Cy
Cuts at: 2692 2692
Size: 5536
EcoRI G'AATT_C
Cuts at: 2845 3223 2845
Size: 378 5158
FspI TGC'GCA
Cuts at: 4042 4042
Size: 5536
HaeII r_GCGC'y
Cuts at: 1678 2013 2446 4683 1678
Size: 335 433 2237 2531
Fragments arranged by size:
2531 2237 433 335
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4177 4209 4251 4283 1373
Size: 32 135 32 2605 32 42 32 2626
Fragments arranged by size:
2626 2605 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2880 3126 669
Size: 988 281 942 246 3079
Fragments arranged by size:
3079 988 942 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5536
HpaI GTT'AAC
Cuts at: 3126 3126
Size: 5536
KpnI G_GTAC'C
Cuts at: 2861 2861
Size: 5536
MfeI C'AATT_G
Cuts at: 3135 3135
Size: 5536
MluI A'CGCG_T
Cuts at: 5412 5412
Size: 5536
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4650 4834 1305
Size: 3345 184 2007
Fragments arranged by size:
3345 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2516 10
Size: 54 1425 343 684 3030
Fragments arranged by size:
3030 1425 684 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2603 2618 3494 3853 4012 518
Size: 648 1437 15 876 359 159 2042
Fragments arranged by size:
2042 1437 876 648 359 159 15
MspA1I CmG'CkG
Cuts at: 1606 2120 2188 2301 2823 2952 3629 4777 5022
Size: 514 68 113 522 129 677 1148 245
Cuts at: 5022 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 522 514 245 129 113
68
NcoI C'CATG_G
Cuts at: 513 1065 2682 513
Size: 552 1617 3367
Fragments arranged by size:
3367 1617 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5536
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5536
NotI GC'GGCC_GC
Cuts at: 2983 2983
Size: 5536
NspI r_CATG'y
Cuts at: 1132 1229 2351 2894 4439 1132
Size: 97 1122 543 1545 2229
Fragments arranged by size:
2229 1545 1122 543 97
PasI CC'CwG_GG
Cuts at: 1348 2415 1348
Size: 1067 4469
PciI A'CATG_T
Cuts at: 4435 4435
Size: 5536
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5536
PfoI T'CCnGG_A
Cuts at: 2094 2094
Size: 5536
PmeI GTTT'AAAC
Cuts at: 2840 2840
Size: 5536
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3605 3637 5118 5150 3605
Size: 32 1481 32 3991
Fragments arranged by size:
3991 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5536
PsiI TTA'TAA
Cuts at: 3106 3106
Size: 5536
PstI C_TGCA'G
Cuts at: 830 2109 2888 830
Size: 1279 779 3478
Fragments arranged by size:
3478 1279 779
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5536
PvuII CAG'CTG
Cuts at: 2120 2823 2120
Size: 703 4833
SalI G'TCGA_C
Cuts at: 1936 2878 1936
Size: 942 4594
SbfI CC_TGCA'GG
Cuts at: 2109 2888 2109
Size: 779 4757
ScaI AGT'ACT
Cuts at: 3784 5496 3784
Size: 1712 3824
SfcI C'TryA_G
Cuts at: 826 1045 2105 2884 4019 4700 4891 826
Size: 219 1060 779 1135 681 191 1471
Fragments arranged by size:
1471 1135 1060 779 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5536
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5536
SmaI CCC'GGG
Cuts at: 1872 2016 2863 1872
Size: 144 847 4545
Fragments arranged by size:
4545 847 144
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2048 2656 2669 3640 4541 4803
Size: 197 926 105 608 13 971 901 262
Cuts at: 4803 820
Size: 1553
Fragments arranged by size:
1553 971 926 901 608 262 197 105
13
SnaBI TAC'GTA
Cuts at: 493 3327 493
Size: 2834 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5536
SphI G_CATG'C
Cuts at: 1132 2894 1132
Size: 1762 3774
SspI AAT'ATT
Cuts at: 5 52 3460 5
Size: 47 3408 2081
Fragments arranged by size:
3408 2081 47
StuI AGG'CCT
Cuts at: 1517 2033 2432 2718 1517
Size: 516 399 286 4335
Fragments arranged by size:
4335 516 399 286
StyI C'CwwG_G
Cuts at: 513 1065 2682 2975 513
Size: 552 1617 293 3074
Fragments arranged by size:
3074 1617 552 293
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2438 2815 3563 3580 3733 3918 4363
Size: 1255 858 377 748 17 153 185 445
Cuts at: 4363 325
Size: 1498
Fragments arranged by size:
1498 1255 858 748 445 377 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3782 5494 96
Size: 274 80 33 51 1443 1805 1712 138
Fragments arranged by size:
1805 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2657 2689 5496 5528 2657
Size: 32 2807 32 2665
Fragments arranged by size:
2807 2665 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5536
XbaI T'CTAG_A
Cuts at: 2872 2872
Size: 5536
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2173 2173
Size: 5536
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5536
XmaI C'CCGG_G
Cuts at: 1870 2014 2861 1870
Size: 144 847 4545
Fragments arranged by size:
4545 847 144
XmnI GAAnn'nnTTC
Cuts at: 3665 3665
Size: 5536
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3344 276
Size: 53 83 186 936 1810 2468
Fragments arranged by size:
2468 1810 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI