Restriction map for
pFN21AB8782
(Circular) (Six-base) MAPSORT of: pFN21AB8782.seq Check: 1977 from: 1 to: 5524
pFN21AB8782 5524 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3334 278
Size: 53 83 186 936 1798 2468
Fragments arranged by size:
2468 1798 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2867 1937
Size: 930 4594
Acc65I G'GTAC_C
Cuts at: 2111 2845 2111
Size: 734 4790
AclI AA'CG_TT
Cuts at: 1563 3651 4024 1563
Size: 2088 373 3063
Fragments arranged by size:
3063 2088 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3587 4971 5239 1632
Size: 1955 1384 268 1917
Fragments arranged by size:
1955 1917 1384 268
AfeI AGC'GCT
Cuts at: 2720 2720
Size: 5524
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5327
AflIII A'CryG_T
Cuts at: 1203 2535 4423 5400 1203
Size: 1332 1888 977 1327
Fragments arranged by size:
1888 1332 1327 977
AgeI A'CCGG_T
Cuts at: 4355 4355
Size: 5524
AhdI GACnn_n'nnGTC
Cuts at: 4253 4253
Size: 5524
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5524
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5524
AlwNI CAG_nnn'CTG
Cuts at: 3267 4839 3267
Size: 1572 3952
ApaLI G'TGCA_C
Cuts at: 2222 2399 3581 4737 2222
Size: 177 1182 1156 3009
Fragments arranged by size:
3009 1182 1156 177
ApoI r'AATT_y
Cuts at: 1493 2833 3059 3211 5320 1493
Size: 1340 226 152 2109 1697
Fragments arranged by size:
2109 1697 1340 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4078 160
Size: 872 3046 1606
Fragments arranged by size:
3046 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5524
AvaI C'yCGr_G
Cuts at: 1870 1943 2543 2705 2849 1870
Size: 73 600 162 144 4545
Fragments arranged by size:
4545 600 162 144 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2095 2128 2095
Size: 33 5491
BamHI G'GATC_C
Cuts at: 2854 3221 2854
Size: 367 5157
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2111 2366 2670 2845 4300
Size: 325 217 627 324 255 304 175 1455
Cuts at: 4300 618
Size: 1842
Fragments arranged by size:
1842 1455 627 325 324 304 255 217
175
BanII G_rGCy'C
Cuts at: 1391 2438 2843 1391
Size: 1047 405 4072
Fragments arranged by size:
4072 1047 405
BbeI G_GCGC'C
Cuts at: 2370 2674 2370
Size: 304 5220
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2221 928
Size: 971 322 4231
Fragments arranged by size:
4231 971 322
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2333 2367 2584 2618 2620 2654 3715
Size: 34 396 34 217 34 2 34 1061
Cuts at: 3715 3749 1903
Size: 34 3678
Fragments arranged by size:
3678 1061 396 217 34 34 34 34
34 2
BciVI GTATCCnnnnn_n'
Cuts at: 3416 4626 3416
Size: 1210 4314
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5524
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4135 136
Size: 107 122 71 631 605 2463 1525
Fragments arranged by size:
2463 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5519 5519
Size: 5524
BlpI GC'TnA_GC
Cuts at: 2432 2524 2941 2432
Size: 92 417 5015
Fragments arranged by size:
5015 417 92
Bme1580I G_kGCm'C
Cuts at: 1790 2226 2403 3585 4741 1790
Size: 436 177 1182 1156 2573
Fragments arranged by size:
2573 1182 1156 436 177
BmgBI CAC'GTC
Cuts at: 1318 2227 1318
Size: 909 4615
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3255 4213 442
Size: 416 53 83 399 24 1838 958 1753
Fragments arranged by size:
1838 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5524
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4184 1239
Size: 2945 2579
Bpu10I CC'TnA_GC
Cuts at: 2504 2504
Size: 5524
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2396 3649 4514 4812 2396
Size: 1253 865 298 3108
Fragments arranged by size:
3108 1253 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4187 882
Size: 617 2688 2219
Fragments arranged by size:
2688 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3315 493
Size: 711 2111 2702
Fragments arranged by size:
2702 2111 711
BsaBI GATnn'nnATC
Cuts at: 5518 5518
Size: 5524
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2367 2601 2671 3331
Size: 53 83 186 936 834 234 70 660
Cuts at: 3331 3713 4348 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 834 660 635 382 234 186
83 70 53
BsaWI w'CCGG_w
Cuts at: 3957 4355 4629 4776 5187 3957
Size: 398 274 147 411 4294
Fragments arranged by size:
4294 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5299
Fragments arranged by size:
5299 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5524
BseYI C'CCAG_C
Cuts at: 2044 4727 5223 2044
Size: 2683 496 2345
Fragments arranged by size:
2683 2345 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2327 2366 1163
Size: 573 591 39 4321
Fragments arranged by size:
4321 591 573 39
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2283 2479 2974 3735 4763 1540
Size: 275 186 282 196 495 761 1028 2301
Fragments arranged by size:
2301 1028 761 495 282 275 196 186
BsiHKAI G_wGCw'C
Cuts at: 2226 2403 2510 2553 2610 2691 3585 3670 4741
Size: 177 107 43 57 81 894 85 1071
Cuts at: 4741 2226
Size: 3009
Fragments arranged by size:
3009 1071 894 177 107 85 81 57
43
BsmI GAATG_Cn'
Cuts at: 3035 3128 3035
Size: 93 5431
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2226 2403 2438 2510 2553 2610 2691
Size: 399 436 177 35 72 43 57 81
Cuts at: 2691 2843 3585 3670 4741 1391
Size: 152 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 436 399 177 152 85
81 72 57 43 35
BspEI T'CCGG_A
Cuts at: 5187 5187
Size: 5524
BspHI T'CATG_A
Cuts at: 3413 5143 3413
Size: 1730 3794
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2301 2388 2879 844
Size: 1457 87 491 3489
Fragments arranged by size:
3489 1457 491 87
BsrBI CCG'CTC
Cuts at: 1394 1823 3411 1394
Size: 429 1588 3507
Fragments arranged by size:
3507 1588 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4019 4193 65
Size: 1183 2771 174 1396
Fragments arranged by size:
2771 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2136 2363 2657 4168 4355 1700
Size: 110 326 227 294 1511 187 2869
Fragments arranged by size:
2869 1511 326 294 227 187 110
BsrGI T'GTAC_A
Cuts at: 96 2262 96
Size: 2166 3358
BssHII G'CGCG_C
Cuts at: 1925 2339 2789 2806 1925
Size: 414 450 17 4643
Fragments arranged by size:
4643 450 414 17
BssSI C'ACGA_G
Cuts at: 3584 4596 3584
Size: 1012 4512
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2221 2305 794
Size: 1427 84 4013
Fragments arranged by size:
4013 1427 84
BstBI TT'CG_AA
Cuts at: 4328 4328
Size: 5524
BstEII G'GTnAC_C
Cuts at: 1184 2620 1184
Size: 1436 4088
BstXI CCAn_nnnn'nTGG
Cuts at: 2128 2489 2128
Size: 361 5163
BstYI r'GATC_y
Cuts at: 1972 2854 3221 3606 3623 5075 5161 5173 5519
Size: 882 367 385 17 1452 86 12 346
Cuts at: 5519 1972
Size: 1977
Fragments arranged by size:
1977 1452 882 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2115 2488 2775 513
Size: 552 1050 373 287 3262
Fragments arranged by size:
3262 1050 552 373 287
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2638 487
Size: 683 56 1412 3373
Fragments arranged by size:
3373 1412 683 56
BtsI GCAGTG_nn'
Cuts at: 3040 3834 3854 3040
Size: 794 20 4710
Fragments arranged by size:
4710 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5524
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5489
DraI TTT'AAA
Cuts at: 2828 2828
Size: 5524
DraIII CAC_nnn'GTG
Cuts at: 2221 2398 2628 2221
Size: 177 230 5117
Fragments arranged by size:
5117 230 177
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4531 809
Size: 507 3215 1802
Fragments arranged by size:
3215 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2088 2118 2280
Size: 54 1425 50 275 18 258 30 162
Cuts at: 2280 2476 2971 3860 5489 8
Size: 196 495 889 1629 43
Fragments arranged by size:
1629 1425 889 495 275 258 196 162
54 50 43 30 18
EagI C'GGCC_G
Cuts at: 1537 1812 2280 2476 2971 1537
Size: 275 468 196 495 4090
Fragments arranged by size:
4090 495 468 275 196
EarI CTCTTCn'nnn_
Cuts at: 1353 3454 1353
Size: 2101 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2752 4111 4485 4631 387
Size: 1064 138 1163 1359 374 146 1280
Fragments arranged by size:
1359 1280 1163 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3587 4184 4971 5239 1239
Size: 393 1955 597 787 268 1524
Fragments arranged by size:
1955 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2728 2769 2728
Size: 41 5483
EcoRI G'AATT_C
Cuts at: 2833 3211 2833
Size: 378 5146
FspI TGC'GCA
Cuts at: 2355 4030 2355
Size: 1675 3849
HaeII r_GCGC'y
Cuts at: 1678 2150 2370 2449 2674 2722 4671 1678
Size: 472 220 79 225 48 1949 2531
Fragments arranged by size:
2531 1949 472 225 220 79 48
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4165 4197 4239 4271 1373
Size: 32 135 32 2593 32 42 32 2626
Fragments arranged by size:
2626 2593 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2164 2868 3114 669
Size: 988 281 226 704 246 3079
Fragments arranged by size:
3079 988 704 281 246 226
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5524
HpaI GTT'AAC
Cuts at: 3114 3114
Size: 5524
KasI G'GCGC_C
Cuts at: 2366 2670 2366
Size: 304 5220
KpnI G_GTAC'C
Cuts at: 2115 2849 2115
Size: 734 4790
MfeI C'AATT_G
Cuts at: 2055 3123 2055
Size: 1068 4456
MluI A'CGCG_T
Cuts at: 2535 5400 2535
Size: 2865 2659
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4638 4822 1305
Size: 3333 184 2007
Fragments arranged by size:
3333 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2090 2120 10
Size: 54 1425 343 258 30 3414
Fragments arranged by size:
3414 1425 343 258 54 30
MslI CAynn'nnrTG
Cuts at: 518 1166 2126 2243 2270 3482 3841 4000 518
Size: 648 960 117 27 1212 359 159 2042
Fragments arranged by size:
2042 1212 960 648 359 159 117 27
MspA1I CmG'CkG
Cuts at: 1606 2777 2940 3617 4765 5010 1606
Size: 1171 163 677 1148 245 2120
Fragments arranged by size:
2120 1171 1148 677 245 163
NaeI GCC'GGC
Cuts at: 2365 2659 2365
Size: 294 5230
NarI GG'CG_CC
Cuts at: 2367 2671 2367
Size: 304 5220
NcoI C'CATG_G
Cuts at: 513 1065 2488 513
Size: 552 1423 3549
Fragments arranged by size:
3549 1423 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5524
NgoMIV G'CCGG_C
Cuts at: 2363 2657 2363
Size: 294 5230
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5524
NotI GC'GGCC_GC
Cuts at: 2280 2971 2280
Size: 691 4833
NspI r_CATG'y
Cuts at: 1132 1229 2635 2882 4427 1132
Size: 97 1406 247 1545 2229
Fragments arranged by size:
2229 1545 1406 247 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5524
PciI A'CATG_T
Cuts at: 4423 4423
Size: 5524
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5524
PmeI GTTT'AAAC
Cuts at: 2828 2828
Size: 5524
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3593 3625 5106 5138 3593
Size: 32 1481 32 3979
Fragments arranged by size:
3979 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5524
PsiI TTA'TAA
Cuts at: 2040 3094 2040
Size: 1054 4470
PspXI vC'TCGA_Gb
Cuts at: 2705 2705
Size: 5524
PstI C_TGCA'G
Cuts at: 830 2751 2876 830
Size: 1921 125 3478
Fragments arranged by size:
3478 1921 125
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5524
SacII CC_GC'GG
Cuts at: 2778 2778
Size: 5524
SalI G'TCGA_C
Cuts at: 1936 2866 1936
Size: 930 4594
SbfI CC_TGCA'GG
Cuts at: 2876 2876
Size: 5524
ScaI AGT'ACT
Cuts at: 3772 5484 3772
Size: 1712 3812
SexAI A'CCwGG_T
Cuts at: 2394 2394
Size: 5524
SfcI C'TryA_G
Cuts at: 826 1045 2747 2872 4007 4688 4879 826
Size: 219 1702 125 1135 681 191 1471
Fragments arranged by size:
1702 1471 1135 681 219 191 125
SfoI GGC'GCC
Cuts at: 2368 2672 2368
Size: 304 5220
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5524
SgrAI Cr'CCGG_yG
Cuts at: 1700 2363 1700
Size: 663 4861
SmaI CCC'GGG
Cuts at: 1872 2851 1872
Size: 979 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2411 2705 3628 4529 4791 820
Size: 197 926 468 294 923 901 262 1553
Fragments arranged by size:
1553 926 923 901 468 294 262 197
SnaBI TAC'GTA
Cuts at: 493 3315 493
Size: 2822 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5524
SphI G_CATG'C
Cuts at: 1132 2635 2882 1132
Size: 1503 247 3774
Fragments arranged by size:
3774 1503 247
SspI AAT'ATT
Cuts at: 5 52 3448 5
Size: 47 3396 2081
Fragments arranged by size:
3396 2081 47
StuI AGG'CCT
Cuts at: 1517 2703 1517
Size: 1186 4338
StyI C'CwwG_G
Cuts at: 513 1065 2076 2488 2963 513
Size: 552 1011 412 475 3074
Fragments arranged by size:
3074 1011 552 475 412
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3551 3568 3721 3906 4351 325
Size: 1255 1971 17 153 185 445 1498
Fragments arranged by size:
1971 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2103 2262 3770
Size: 274 80 33 51 1443 126 159 1508
Cuts at: 3770 5482 96
Size: 1712 138
Fragments arranged by size:
1712 1508 1443 274 159 138 126 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5484 5516 5484
Size: 32 5492
Tth111I GACn'n_nGTC
Cuts at: 1316 2159 1316
Size: 843 4681
XbaI T'CTAG_A
Cuts at: 2860 2860
Size: 5524
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2083 2128 2083
Size: 45 5479
XhoI C'TCGA_G
Cuts at: 1943 2705 1943
Size: 762 4762
XmaI C'CCGG_G
Cuts at: 1870 2849 1870
Size: 979 4545
XmnI GAAnn'nnTTC
Cuts at: 3653 3653
Size: 5524
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3332 276
Size: 53 83 186 936 1798 2468
Fragments arranged by size:
2468 1798 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspXI PstI PvuI SacII
SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BbvCI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI
FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PsrI PvuII RsrII SacI SanDI SapI SfiI
SrfI SwaI